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The map label for this gene is rffG [H]

Identifier: 148259628

GI number: 148259628

Start: 688200

End: 689270

Strand: Reverse

Name: rffG [H]

Synonym: Acry_0614

Alternate gene names: 148259628

Gene position: 689270-688200 (Counterclockwise)

Preceding gene: 148259629

Following gene: 148259627

Centisome position: 20.34

GC content: 70.49

Gene sequence:

>1071_bases
ATGCCTGATCCAACCTTGCGATTTCTGATCACCGGGGGCTGCGGCTTCATTGGTTCTGCGGTTGTCCGTCGGCTTGTGGC
GAGCACGCCGCACGAGGTCGTCACCGTCGACAAGATGACCTACGCCGCCTCGGAGGACGCGCTGGGCGCTGCGCTGGGGC
ATCCGCGCCATCGCCTGATCCGCGCCGACATCACCGATGCAGCGGCGATGCGCGCCGCCTTCGAGGCGCACCGGCCCGAC
ATCGTCATGCATCTCGCCGCCGAATCCCATGTCGACCGCTCGATCGACGGGCCGCGCGATTTCATCGACACCAACATCAC
CGGCACCTTCGTCCTGCTCGAGGCCGCCCGCGCCTGGTTCGCCACCCTGGACGAGCCCGCCCGCCGCCGCTTCCGCTTCC
ACCACATCTCCACCGACGAGGTGTTCGGCGCCCTCGAACAAGGCGATCCCCCCTTCACCGAAACCACCCCCTACGACCCC
CGCAGCCCCTACGCCGCCAGCAAGGCCGCCTCGGACCATCTCGTCCGCGCCTGGCGGCACACCTACGGCCTGCCCGCCAT
CGTCTCCAACACCACCAACAATTACGGCCCCTGGCAGTTCCCCGAGAAACTCATCCCGCTGGTGCTCATCAACGCCCTCG
AGGGCCGCCCCCTGCCCGTCTATGGCGACGGCTCCAACCTGCGCGACTGGCTCTTCGTCGAGGATCACGCCGAGGCGCTC
CTCGCCATCGCCCTCACCGGCACGCCCGGCGAGACCTACGCCATCGGCGCCCGCCAGCCCCGCTCCAATCTCGACGTCGT
CCGCGCCATCTGCCGCCTGCTCGATGCCCGCCGCCCCGATCCCGCCGGCCCGCGCGAGCGGCTGATCACCTTCGTCGCCG
ACCGCCCCGGCCACGATTTCCGCTACGAGATCGACCCCGCCCGCAGCGAGGCCGCCCTCGCCTGGCGCGCCGCCCACGAT
TTCGAGGCCGGACTCGCCCGCACCGTCGACTGGTATCTCGCCAACGAGCCCTGGTGGCGCGCCATCCGCGCCCGCCGCTA
TGCCGGCCAGCGCCTCGGTACCGCCGCATGA

Upstream 100 bases:

>100_bases
GGCCGCATCCGTCCCCGTCGCATCACGACACGATTCCGACGTTCCGCATTTGCAAACATCTTGCGCGAAGCAGATTTCCT
GATCCGCTCTTTTTGAGATT

Downstream 100 bases:

>100_bases
TCCGCAAGGGCATCATCCTCGCCGGCGGCTCCGGCACCCGCCTGCACCCCTCCACCCTCGCCGCCTCGAAGCAGCTCCTC
CCCGTCTACGACAAGCCGAT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MPDPTLRFLITGGCGFIGSAVVRRLVASTPHEVVTVDKMTYAASEDALGAALGHPRHRLIRADITDAAAMRAAFEAHRPD
IVMHLAAESHVDRSIDGPRDFIDTNITGTFVLLEAARAWFATLDEPARRRFRFHHISTDEVFGALEQGDPPFTETTPYDP
RSPYAASKAASDHLVRAWRHTYGLPAIVSNTTNNYGPWQFPEKLIPLVLINALEGRPLPVYGDGSNLRDWLFVEDHAEAL
LAIALTGTPGETYAIGARQPRSNLDVVRAICRLLDARRPDPAGPRERLITFVADRPGHDFRYEIDPARSEAALAWRAAHD
FEAGLARTVDWYLANEPWWRAIRARRYAGQRLGTAA

Sequences:

>Translated_356_residues
MPDPTLRFLITGGCGFIGSAVVRRLVASTPHEVVTVDKMTYAASEDALGAALGHPRHRLIRADITDAAAMRAAFEAHRPD
IVMHLAAESHVDRSIDGPRDFIDTNITGTFVLLEAARAWFATLDEPARRRFRFHHISTDEVFGALEQGDPPFTETTPYDP
RSPYAASKAASDHLVRAWRHTYGLPAIVSNTTNNYGPWQFPEKLIPLVLINALEGRPLPVYGDGSNLRDWLFVEDHAEAL
LAIALTGTPGETYAIGARQPRSNLDVVRAICRLLDARRPDPAGPRERLITFVADRPGHDFRYEIDPARSEAALAWRAAHD
FEAGLARTVDWYLANEPWWRAIRARRYAGQRLGTAA
>Mature_355_residues
PDPTLRFLITGGCGFIGSAVVRRLVASTPHEVVTVDKMTYAASEDALGAALGHPRHRLIRADITDAAAMRAAFEAHRPDI
VMHLAAESHVDRSIDGPRDFIDTNITGTFVLLEAARAWFATLDEPARRRFRFHHISTDEVFGALEQGDPPFTETTPYDPR
SPYAASKAASDHLVRAWRHTYGLPAIVSNTTNNYGPWQFPEKLIPLVLINALEGRPLPVYGDGSNLRDWLFVEDHAEALL
AIALTGTPGETYAIGARQPRSNLDVVRAICRLLDARRPDPAGPRERLITFVADRPGHDFRYEIDPARSEAALAWRAAHDF
EAGLARTVDWYLANEPWWRAIRARRYAGQRLGTAA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=340, Percent_Identity=33.2352941176471, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=24.1691842900302, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=56.5714285714286, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1788353, Length=350, Percent_Identity=55.4285714285714, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1786974, Length=367, Percent_Identity=26.4305177111717, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788366, Length=250, Percent_Identity=28.8, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=340, Percent_Identity=35.2941176470588, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI115532424, Length=359, Percent_Identity=30.9192200557103, Blast_Score=157, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17539532, Length=348, Percent_Identity=24.4252873563218, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=276, Percent_Identity=27.536231884058, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI133901788, Length=225, Percent_Identity=28.8888888888889, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17539422, Length=225, Percent_Identity=28.8888888888889, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=225, Percent_Identity=28.8888888888889, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI133901790, Length=225, Percent_Identity=28.8888888888889, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17539424, Length=225, Percent_Identity=28.8888888888889, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19923002, Length=368, Percent_Identity=28.804347826087, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356223, Length=332, Percent_Identity=25.6024096385542, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24158427, Length=224, Percent_Identity=25, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39542; Mature: 39411

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDPTLRFLITGGCGFIGSAVVRRLVASTPHEVVTVDKMTYAASEDALGAALGHPRHRLI
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHHHHCCCHHHEE
RADITDAAAMRAAFEAHRPDIVMHLAAESHVDRSIDGPRDFIDTNITGTFVLLEAARAWF
ECCCHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHH
ATLDEPARRRFRFHHISTDEVFGALEQGDPPFTETTPYDPRSPYAASKAASDHLVRAWRH
HHCCHHHHHCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TYGLPAIVSNTTNNYGPWQFPEKLIPLVLINALEGRPLPVYGDGSNLRDWLFVEDHAEAL
HCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEHHCCEE
LAIALTGTPGETYAIGARQPRSNLDVVRAICRLLDARRPDPAGPRERLITFVADRPGHDF
EEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEEEEECCCCCCC
RYEIDPARSEAALAWRAAHDFEAGLARTVDWYLANEPWWRAIRARRYAGQRLGTAA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PDPTLRFLITGGCGFIGSAVVRRLVASTPHEVVTVDKMTYAASEDALGAALGHPRHRLI
CCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHHHHCCCHHHEE
RADITDAAAMRAAFEAHRPDIVMHLAAESHVDRSIDGPRDFIDTNITGTFVLLEAARAWF
ECCCHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHH
ATLDEPARRRFRFHHISTDEVFGALEQGDPPFTETTPYDPRSPYAASKAASDHLVRAWRH
HHCCHHHHHCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TYGLPAIVSNTTNNYGPWQFPEKLIPLVLINALEGRPLPVYGDGSNLRDWLFVEDHAEAL
HCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEHHCCEE
LAIALTGTPGETYAIGARQPRSNLDVVRAICRLLDARRPDPAGPRERLITFVADRPGHDF
EEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEEEEECCCCCCC
RYEIDPARSEAALAWRAAHDFEAGLARTVDWYLANEPWWRAIRARRYAGQRLGTAA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]