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The map label for this gene is levD [H]

Identifier: 148259462

GI number: 148259462

Start: 496105

End: 496527

Strand: Reverse

Name: levD [H]

Synonym: Acry_0445

Alternate gene names: 148259462

Gene position: 496527-496105 (Counterclockwise)

Preceding gene: 148259463

Following gene: 148259461

Centisome position: 14.65

GC content: 60.05

Gene sequence:

>423_bases
ATGATCGGTATGGTGCTCGTAAGCCATGGCAAGCTTGGCGTCGCTCTCCGCGAGGCGCTGGAGCATGTCGTCGGTCCGCA
GAGAAACATCGCGACCGTCTCCGTCGAGGCGGATGCCGATATTGAAACCCAGCGCAAGGAAATTGCCGATCGCGTTGCCG
ATGTCGATACAGGTGACGGCGTGGTGGTTCTGACCGACATGTTCGGAGGAACGCCGTGCAACCTGTCGATGAGCATGCTG
GAGCGCGACGACGTGGAGGTAATCGCCGGGGTCAACCTGCCGATGCTGGTCAAGCTGGCAAAGGTCCGCGGCTCACATAG
CCTGGCAGATGCCGTTCATTGCGCGGAAGAAGCCGGTCGAAAATACATTGCCTGTGGATCAGAGATCCTCCACCCCGTCA
CACCGAAGCGTGCGTGCGGATGA

Upstream 100 bases:

>100_bases
TCCTGATTGAGCATCGCAGCCTCGGCTCCAGATCCGTAGGCGGTGCGTGGAAGGCGATGCCGGGAGCCCATCAAGGTGCT
CCGATGCCCGGAGTCCAGAA

Downstream 100 bases:

>100_bases
GCGCCGATCATTCGCCCGTTCGCTTGGCCCAGACGGTCACCATCTGCAATGCCCGCGGCCTTCATGCGCGCGCCGCAGCC
AAATTGGTGACTTTGGCCGA

Product: PTS system fructose subfamily IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Fru; PTS system fructose-specific EIIA component; p16 [H]

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MIGMVLVSHGKLGVALREALEHVVGPQRNIATVSVEADADIETQRKEIADRVADVDTGDGVVVLTDMFGGTPCNLSMSML
ERDDVEVIAGVNLPMLVKLAKVRGSHSLADAVHCAEEAGRKYIACGSEILHPVTPKRACG

Sequences:

>Translated_140_residues
MIGMVLVSHGKLGVALREALEHVVGPQRNIATVSVEADADIETQRKEIADRVADVDTGDGVVVLTDMFGGTPCNLSMSML
ERDDVEVIAGVNLPMLVKLAKVRGSHSLADAVHCAEEAGRKYIACGSEILHPVTPKRACG
>Mature_140_residues
MIGMVLVSHGKLGVALREALEHVVGPQRNIATVSVEADADIETQRKEIADRVADVDTGDGVVVLTDMFGGTPCNLSMSML
ERDDVEVIAGVNLPMLVKLAKVRGSHSLADAVHCAEEAGRKYIACGSEILHPVTPKRACG

Specific function: LevD and levE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator [H]

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=122, Percent_Identity=33.6065573770492, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789 [H]

Pfam domain/function: PF03610 EIIA-man [H]

EC number: 2.7.1.69

Molecular weight: Translated: 14816; Mature: 14816

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGMVLVSHGKLGVALREALEHVVGPQRNIATVSVEADADIETQRKEIADRVADVDTGDG
CCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCC
VVVLTDMFGGTPCNLSMSMLERDDVEVIAGVNLPMLVKLAKVRGSHSLADAVHCAEEAGR
EEEEEECCCCCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCC
KYIACGSEILHPVTPKRACG
EEEECCHHHHCCCCCCCCCC
>Mature Secondary Structure
MIGMVLVSHGKLGVALREALEHVVGPQRNIATVSVEADADIETQRKEIADRVADVDTGDG
CCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCC
VVVLTDMFGGTPCNLSMSMLERDDVEVIAGVNLPMLVKLAKVRGSHSLADAVHCAEEAGR
EEEEEECCCCCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCC
KYIACGSEILHPVTPKRACG
EEEECCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377 [H]