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The map label for this gene is dsbA [C]

Identifier: 148259417

GI number: 148259417

Start: 457322

End: 457951

Strand: Reverse

Name: dsbA [C]

Synonym: Acry_0400

Alternate gene names: 148259417

Gene position: 457951-457322 (Counterclockwise)

Preceding gene: 148259424

Following gene: 148259416

Centisome position: 13.51

GC content: 60.63

Gene sequence:

>630_bases
ATGGACCGTCGTTCGCTACTAGGAATGGTAGGTGGGCTTGCGACCGCAGGCTCTCTGGGGATCGCCTTTCCGGCGGCCGC
CGCGGAAACGAAAACCGATTCCCCCTATTCGATCCGCAGCCTCGGCAATCCGAATGCCCCCGTAACCGTCTATGAATATT
TTTCCCTCAACTGTCCGCATTGCGCCGAATTCGCCACGCACGCGCTGCCCAAGGTCATCGAAAGCTACGTCAAGCCAGGC
AAGGTCTATTACGTCTTCAAGGACTTCCCGCTGAACGAGGACGCACTCTGGGCCGCGCAGATCGCCCGCGCGCTGCCGGC
GAAGGCATATTATCCGTTCATCTCGGAACTCTTCCGCACGCAGGATGAATGGGCCTACGCGCCGGGGCTGAAAACGCCCA
AGGATTACCAGAACGCCCTGTTCCGCTACGCCGCCCTCGCCGGCATGGACCGCACGACGTTCGACGCCGCCATAGCCAAC
AAGAAGCTGCGCGCCTTCGTCCTCAACGAACTGAACGACGCCGAGAAGACCTACAAGGTCAATTCGACCCCGACCTTCAT
CATCAATGGCCGCAAGCGGGAGGGAGCGGTTTCGTTCGACACCTTCTCCTCCTGGCTGAAGGCCGCGTAG

Upstream 100 bases:

>100_bases
TCCTGGGGCGATCGGCTATGCATCGGACCAGCACAGGCGCCGTTTCCGCTCGACGTGGCGGAACGCTTCCGCTACACAAC
ATATAGAGGTAGATATCGTC

Downstream 100 bases:

>100_bases
GTCCGGCTTGCCCGCGCGTTTCGCCCGCCTGCGGATCAGCGGGTTCAAGAGTTTCGCCGATCCGACCACGATCGACATCC
TGCCAGGGCTGACCGGCATC

Product: protein-disulfide isomerase-like protein

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MDRRSLLGMVGGLATAGSLGIAFPAAAAETKTDSPYSIRSLGNPNAPVTVYEYFSLNCPHCAEFATHALPKVIESYVKPG
KVYYVFKDFPLNEDALWAAQIARALPAKAYYPFISELFRTQDEWAYAPGLKTPKDYQNALFRYAALAGMDRTTFDAAIAN
KKLRAFVLNELNDAEKTYKVNSTPTFIINGRKREGAVSFDTFSSWLKAA

Sequences:

>Translated_209_residues
MDRRSLLGMVGGLATAGSLGIAFPAAAAETKTDSPYSIRSLGNPNAPVTVYEYFSLNCPHCAEFATHALPKVIESYVKPG
KVYYVFKDFPLNEDALWAAQIARALPAKAYYPFISELFRTQDEWAYAPGLKTPKDYQNALFRYAALAGMDRTTFDAAIAN
KKLRAFVLNELNDAEKTYKVNSTPTFIINGRKREGAVSFDTFSSWLKAA
>Mature_209_residues
MDRRSLLGMVGGLATAGSLGIAFPAAAAETKTDSPYSIRSLGNPNAPVTVYEYFSLNCPHCAEFATHALPKVIESYVKPG
KVYYVFKDFPLNEDALWAAQIARALPAKAYYPFISELFRTQDEWAYAPGLKTPKDYQNALFRYAALAGMDRTTFDAAIAN
KKLRAFVLNELNDAEKTYKVNSTPTFIINGRKREGAVSFDTFSSWLKAA

Specific function: May be required for disulfide bond formation in some proteins [H]

COG id: COG1651

COG function: function code O; Protein-disulfide isomerase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23103; Mature: 23103

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRSLLGMVGGLATAGSLGIAFPAAAAETKTDSPYSIRSLGNPNAPVTVYEYFSLNCPH
CCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCH
CAEFATHALPKVIESYVKPGKVYYVFKDFPLNEDALWAAQIARALPAKAYYPFISELFRT
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHC
QDEWAYAPGLKTPKDYQNALFRYAALAGMDRTTFDAAIANKKLRAFVLNELNDAEKTYKV
CCCCEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEC
NSTPTFIINGRKREGAVSFDTFSSWLKAA
CCCCEEEEECCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MDRRSLLGMVGGLATAGSLGIAFPAAAAETKTDSPYSIRSLGNPNAPVTVYEYFSLNCPH
CCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCH
CAEFATHALPKVIESYVKPGKVYYVFKDFPLNEDALWAAQIARALPAKAYYPFISELFRT
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHC
QDEWAYAPGLKTPKDYQNALFRYAALAGMDRTTFDAAIANKKLRAFVLNELNDAEKTYKV
CCCCEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEC
NSTPTFIINGRKREGAVSFDTFSSWLKAA
CCCCEEEEECCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]