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The map label for this gene is macA [H]

Identifier: 148259326

GI number: 148259326

Start: 355316

End: 356557

Strand: Reverse

Name: macA [H]

Synonym: Acry_0307

Alternate gene names: 148259326

Gene position: 356557-355316 (Counterclockwise)

Preceding gene: 148259327

Following gene: 148259325

Centisome position: 10.52

GC content: 72.06

Gene sequence:

>1242_bases
ATGACGGTCTCACCGAACCCGCCAGCGAACACGCAGCGCCCACCGCCTTCCGCGCCGCCGGAACCGGCAGCCGCGCCGCG
CCGCCGGCGCGTCCGGCGCGGCGCCGTGCTGGCGGCGCTGGCGGTGGGGGTGGCGGCGGCGATCGCGATCGGCTGGCGGA
CGGTGGGGGCGCCGCCGCCGGTTCACTACGTGACGGCGAAGGTCGGGCGCGGCGACATTACGCGGGCGGTGACGGCGACG
GGGACGGTCAACCCGATCCTGACGGTGATCGTCGGCTCGGCGGTGTCCGGCATCATCCAGGACATCAGCTGCGACTACAA
CACGATCGTGAAGCTCGGGCAGATCTGCGCCCGGATCGACCCGCGGCCCTACCAGGCGGTGGTGGACCAGGACCGGGCGG
CGGTGGCCGTGGACCAGGCGCAGCTGCGCAAGGACGAGGCGAGCCTCGACTATGCGCGCGGCAATTACCGCCGCCTCGCC
TCGCTGGTGCCGCAGCGCTACGCCTCGGAGAACGCCGCCGACAGCGCGAAGGCGACGTATCGGCAACTGGAGGCGCAGAC
CGCGCTCGACCGCGCGGTGATCCGGCAGGACGAGGCGAAGCTCGCCGCCGCGCAGGCCAATCTCGGCTATACCGACATCG
TCTCGCCGGTGAACGGGATCGTCGTCTCGCGCAATGTCACGATCGGGCAGACCGTCGCCGCCTCGTTCCAGACGCCGACG
CTGTTCCTGATCGCCACCGACCTGACGCGGATGCAGGTGGATACCAATGTCAGCGAGGGGGATATCGCGGATATCCGCAA
GGGCGAGGCGGCGCGGTTCACCGTTCTCGCCTTTCCGGGCGAGACGTTCGACGGCACGGTGACGCAGGTGCGCCAGTCGC
CGCAGACGGTGCAGAACGTGGTGACCTACGACGTCGTCATCGAGGTGGACAACCGCGACCTGAAGCTGAAGCCGGGCATG
ACCGCGTCGGTGCGGATCATCACCGCGGCGCGGGCCGGCGTGCTGCGCGTGCCGGACCAGGCGCTGCGCTTCACGCCGCG
CCTGGCGAAGGCGGCGCCGGCGGGGGGCGGGGCGCGCGTGTGGGTGCTGCGGCAGGGCAAGCCGGCCGCCGTTCCGGTGG
TCACGGGGCTCGATGACGACACCAATACGGAGATCGTGAGCGGCGGACTGCGCGACGGCGACGAGGTGATCACCGGCGAG
CGGCGGGCGCGGGCGGCGACGCCCTTCGTGCCGCGGTTCTGA

Upstream 100 bases:

>100_bases
ATGCCGGGGTCAGGGAGCGTTGATCTGCGTCATCGCCGCCTGCCCCGCGCGGTCCTAGGTTCGAGCCGATCCGGACGGGC
ACGCGCCCGAAGGAAGCACC

Downstream 100 bases:

>100_bases
CGGGGCGCGGGCGATGCCGGGGGCGGTGCCAGGGGCGGTCATCGAGCTGCGCGGCGTTTCGCGGACCTACCGGAGCGGCG
ATGTCGAGGTGCAGGCGCTG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTVSPNPPANTQRPPPSAPPEPAAAPRRRRVRRGAVLAALAVGVAAAIAIGWRTVGAPPPVHYVTAKVGRGDITRAVTAT
GTVNPILTVIVGSAVSGIIQDISCDYNTIVKLGQICARIDPRPYQAVVDQDRAAVAVDQAQLRKDEASLDYARGNYRRLA
SLVPQRYASENAADSAKATYRQLEAQTALDRAVIRQDEAKLAAAQANLGYTDIVSPVNGIVVSRNVTIGQTVAASFQTPT
LFLIATDLTRMQVDTNVSEGDIADIRKGEAARFTVLAFPGETFDGTVTQVRQSPQTVQNVVTYDVVIEVDNRDLKLKPGM
TASVRIITAARAGVLRVPDQALRFTPRLAKAAPAGGGARVWVLRQGKPAAVPVVTGLDDDTNTEIVSGGLRDGDEVITGE
RRARAATPFVPRF

Sequences:

>Translated_413_residues
MTVSPNPPANTQRPPPSAPPEPAAAPRRRRVRRGAVLAALAVGVAAAIAIGWRTVGAPPPVHYVTAKVGRGDITRAVTAT
GTVNPILTVIVGSAVSGIIQDISCDYNTIVKLGQICARIDPRPYQAVVDQDRAAVAVDQAQLRKDEASLDYARGNYRRLA
SLVPQRYASENAADSAKATYRQLEAQTALDRAVIRQDEAKLAAAQANLGYTDIVSPVNGIVVSRNVTIGQTVAASFQTPT
LFLIATDLTRMQVDTNVSEGDIADIRKGEAARFTVLAFPGETFDGTVTQVRQSPQTVQNVVTYDVVIEVDNRDLKLKPGM
TASVRIITAARAGVLRVPDQALRFTPRLAKAAPAGGGARVWVLRQGKPAAVPVVTGLDDDTNTEIVSGGLRDGDEVITGE
RRARAATPFVPRF
>Mature_412_residues
TVSPNPPANTQRPPPSAPPEPAAAPRRRRVRRGAVLAALAVGVAAAIAIGWRTVGAPPPVHYVTAKVGRGDITRAVTATG
TVNPILTVIVGSAVSGIIQDISCDYNTIVKLGQICARIDPRPYQAVVDQDRAAVAVDQAQLRKDEASLDYARGNYRRLAS
LVPQRYASENAADSAKATYRQLEAQTALDRAVIRQDEAKLAAAQANLGYTDIVSPVNGIVVSRNVTIGQTVAASFQTPTL
FLIATDLTRMQVDTNVSEGDIADIRKGEAARFTVLAFPGETFDGTVTQVRQSPQTVQNVVTYDVVIEVDNRDLKLKPGMT
ASVRIITAARAGVLRVPDQALRFTPRLAKAAPAGGGARVWVLRQGKPAAVPVVTGLDDDTNTEIVSGGLRDGDEVITGER
RARAATPFVPRF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=381, Percent_Identity=33.5958005249344, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI87082036, Length=330, Percent_Identity=25.4545454545455, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790519, Length=241, Percent_Identity=28.6307053941909, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43862; Mature: 43731

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSPNPPANTQRPPPSAPPEPAAAPRRRRVRRGAVLAALAVGVAAAIAIGWRTVGAPPP
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCC
VHYVTAKVGRGDITRAVTATGTVNPILTVIVGSAVSGIIQDISCDYNTIVKLGQICARID
CEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
PRPYQAVVDQDRAAVAVDQAQLRKDEASLDYARGNYRRLASLVPQRYASENAADSAKATY
CCHHHHHHCCCCCEEEHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHH
RQLEAQTALDRAVIRQDEAKLAAAQANLGYTDIVSPVNGIVVSRNVTIGQTVAASFQTPT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCEECHHHHHCCCCCE
LFLIATDLTRMQVDTNVSEGDIADIRKGEAARFTVLAFPGETFDGTVTQVRQSPQTVQNV
EEEEEECCEEEEECCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCHHHHHHCCHHHHHHH
VTYDVVIEVDNRDLKLKPGMTASVRIITAARAGVLRVPDQALRFTPRLAKAAPAGGGARV
EEEEEEEEECCCCEEECCCCCEEEEEEEECCCCEEECCHHHHHCCCHHHHCCCCCCCEEE
WVLRQGKPAAVPVVTGLDDDTNTEIVSGGLRDGDEVITGERRARAATPFVPRF
EEEECCCCCEEEEEECCCCCCCCCEEECCCCCCCHHCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
TVSPNPPANTQRPPPSAPPEPAAAPRRRRVRRGAVLAALAVGVAAAIAIGWRTVGAPPP
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCC
VHYVTAKVGRGDITRAVTATGTVNPILTVIVGSAVSGIIQDISCDYNTIVKLGQICARID
CEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
PRPYQAVVDQDRAAVAVDQAQLRKDEASLDYARGNYRRLASLVPQRYASENAADSAKATY
CCHHHHHHCCCCCEEEHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHH
RQLEAQTALDRAVIRQDEAKLAAAQANLGYTDIVSPVNGIVVSRNVTIGQTVAASFQTPT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCEECHHHHHCCCCCE
LFLIATDLTRMQVDTNVSEGDIADIRKGEAARFTVLAFPGETFDGTVTQVRQSPQTVQNV
EEEEEECCEEEEECCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCHHHHHHCCHHHHHHH
VTYDVVIEVDNRDLKLKPGMTASVRIITAARAGVLRVPDQALRFTPRLAKAAPAGGGARV
EEEEEEEEECCCCEEECCCCCEEEEEEEECCCCEEECCHHHHHCCCHHHHCCCCCCCEEE
WVLRQGKPAAVPVVTGLDDDTNTEIVSGGLRDGDEVITGERRARAATPFVPRF
EEEECCCCCEEEEEECCCCCCCCCEEECCCCCCCHHCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]