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The map label for this gene is metF [H]

Identifier: 148259239

GI number: 148259239

Start: 253851

End: 254783

Strand: Reverse

Name: metF [H]

Synonym: Acry_0219

Alternate gene names: 148259239

Gene position: 254783-253851 (Counterclockwise)

Preceding gene: 148259248

Following gene: 148259238

Centisome position: 7.52

GC content: 69.45

Gene sequence:

>933_bases
ATGAGTCACACCCCGCCCCGCGCCGTCCTGGAACGCTGGCTGTCCGGTCCGCGCGTTGATCCGCTGCCAACGCCTGCGCT
GCGCGCCCCGGCCCCGGCGATCTCGTTCGAATTCTTTCCGCCCAAGACCGAAACACTGGAAGCCCAGCTCTGGGCCTGCA
TTCGCCGGCTCGAACCGCTGGCGCCGCGCTTCGTCTCGATCACCTACGGCGCCGGCGGCTCGACCCAGGAGCGCACCCAC
GCCACCGTCACCCGCCTGGTGCGGGAGACCACCCTTGTTCCGGCAGCGCACCTGACCTGCGTCGGCGCCTCGCGCGGCGA
GGTCGATGATGTCGCCCGCCGCTACTGGGATGCCGGCGTGCGCCACATCGTCGCCCTGCGCGGCGACCCGCCGGGCGGCA
CCGCCTATGCGCCGCATCCCGAGGGCTACGCCTATGCGGCCGATCTCGTCGCCGGGCTGCGCCGCGTCGCCGATTTCGAG
ATCTCGGTCGCCGCCTATCCCGAAACCCACACCCAGGCACCAAGCCCGCAGGCCGATCTCGACAATCTGAAGCGCAAGCT
CGATGCCGGGGCGACGCGGGCGATCAGCCAGGTCTTCTTCGAGACCGGCCATTTCCTCGACTTTCTCGACCGCGCGCTGG
CCGCCGGCATCACCGCGCCGATCATCCCCGGCATCATGCCGGTCTCCAATTTCGGGCAGGCGAAGCGGATCAGCGAGATG
GCCGGCACCCATGTCCCCGCCTGGATGGGCGAGATGTTCGACGGACTCGACGACGATGCCGAAACCCGCAAGATGGTCGC
CGCGAGCATCGCCGCCGAGCAGGTCCGCACGCTGCAGGCCAACGGCATCGACGAGTTCCATTTCTACACCCTCAACCGCG
CCGACCTCACCTACGCCATCGCGCGCATCCTGGGCGTGAAGCCGGCCAGGTGA

Upstream 100 bases:

>100_bases
GTAGCGCAGCATCCGGAGCGATCAACAGCACGGCCCACCGGAGGCTGCCGGCCTTGCCACCGGCGGCGCTTGCTGCCACG
AGACGCCCGAGGAGTTTCCG

Downstream 100 bases:

>100_bases
GCGACTATCTCGACAGGCTGAACCCCGCCCAGCGCGAAGCCGTCGAGGCGGTGGACGGGCCGGTGCTGGTGCTTGCCGGT
GCCGGCACCGGCAAGACCCG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSHTPPRAVLERWLSGPRVDPLPTPALRAPAPAISFEFFPPKTETLEAQLWACIRRLEPLAPRFVSITYGAGGSTQERTH
ATVTRLVRETTLVPAAHLTCVGASRGEVDDVARRYWDAGVRHIVALRGDPPGGTAYAPHPEGYAYAADLVAGLRRVADFE
ISVAAYPETHTQAPSPQADLDNLKRKLDAGATRAISQVFFETGHFLDFLDRALAAGITAPIIPGIMPVSNFGQAKRISEM
AGTHVPAWMGEMFDGLDDDAETRKMVAASIAAEQVRTLQANGIDEFHFYTLNRADLTYAIARILGVKPAR

Sequences:

>Translated_310_residues
MSHTPPRAVLERWLSGPRVDPLPTPALRAPAPAISFEFFPPKTETLEAQLWACIRRLEPLAPRFVSITYGAGGSTQERTH
ATVTRLVRETTLVPAAHLTCVGASRGEVDDVARRYWDAGVRHIVALRGDPPGGTAYAPHPEGYAYAADLVAGLRRVADFE
ISVAAYPETHTQAPSPQADLDNLKRKLDAGATRAISQVFFETGHFLDFLDRALAAGITAPIIPGIMPVSNFGQAKRISEM
AGTHVPAWMGEMFDGLDDDAETRKMVAASIAAEQVRTLQANGIDEFHFYTLNRADLTYAIARILGVKPAR
>Mature_309_residues
SHTPPRAVLERWLSGPRVDPLPTPALRAPAPAISFEFFPPKTETLEAQLWACIRRLEPLAPRFVSITYGAGGSTQERTHA
TVTRLVRETTLVPAAHLTCVGASRGEVDDVARRYWDAGVRHIVALRGDPPGGTAYAPHPEGYAYAADLVAGLRRVADFEI
SVAAYPETHTQAPSPQADLDNLKRKLDAGATRAISQVFFETGHFLDFLDRALAAGITAPIIPGIMPVSNFGQAKRISEMA
GTHVPAWMGEMFDGLDDDAETRKMVAASIAAEQVRTLQANGIDEFHFYTLNRADLTYAIARILGVKPAR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=280, Percent_Identity=31.7857142857143, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1790377, Length=275, Percent_Identity=56, Blast_Score=323, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI25147441, Length=281, Percent_Identity=31.3167259786477, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25147436, Length=281, Percent_Identity=31.3167259786477, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325234, Length=297, Percent_Identity=34.3434343434343, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321313, Length=287, Percent_Identity=29.9651567944251, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24662658, Length=272, Percent_Identity=25.3676470588235, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33691; Mature: 33559

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTPPRAVLERWLSGPRVDPLPTPALRAPAPAISFEFFPPKTETLEAQLWACIRRLEPL
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCC
APRFVSITYGAGGSTQERTHATVTRLVRETTLVPAAHLTCVGASRGEVDDVARRYWDAGV
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCHHHHHHHHHHHHH
RHIVALRGDPPGGTAYAPHPEGYAYAADLVAGLRRVADFEISVAAYPETHTQAPSPQADL
HEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHH
DNLKRKLDAGATRAISQVFFETGHFLDFLDRALAAGITAPIIPGIMPVSNFGQAKRISEM
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHH
AGTHVPAWMGEMFDGLDDDAETRKMVAASIAAEQVRTLQANGIDEFHFYTLNRADLTYAI
HCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHH
ARILGVKPAR
HHHHCCCCCC
>Mature Secondary Structure 
SHTPPRAVLERWLSGPRVDPLPTPALRAPAPAISFEFFPPKTETLEAQLWACIRRLEPL
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCC
APRFVSITYGAGGSTQERTHATVTRLVRETTLVPAAHLTCVGASRGEVDDVARRYWDAGV
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCHHHHHHHHHHHHH
RHIVALRGDPPGGTAYAPHPEGYAYAADLVAGLRRVADFEISVAAYPETHTQAPSPQADL
HEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHH
DNLKRKLDAGATRAISQVFFETGHFLDFLDRALAAGITAPIIPGIMPVSNFGQAKRISEM
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHH
AGTHVPAWMGEMFDGLDDDAETRKMVAASIAAEQVRTLQANGIDEFHFYTLNRADLTYAI
HCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHH
ARILGVKPAR
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]