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The map label for this gene is dfrA [H]

Identifier: 148259141

GI number: 148259141

Start: 133425

End: 134510

Strand: Reverse

Name: dfrA [H]

Synonym: Acry_0121

Alternate gene names: 148259141

Gene position: 134510-133425 (Counterclockwise)

Preceding gene: 148259145

Following gene: 148259140

Centisome position: 3.97

GC content: 66.21

Gene sequence:

>1086_bases
ATGGCAGGCATGAGCAGCGATTCTTCTTCTCCGCTCGAGACGGTTGCGCGCCCCATCGCCGACGACCGGCCGGTTCTTGT
CACCGGCGCGACCGGCTTCGTCGGCGCCGCCGTGGCCCGAGCGCTGGTCCGGCACGGTTTCAGGCTGCGGCTGATGCACC
GCGCTTCGAGCGACATGCGCAATCTCGCGCAACTGCCCGGCGAGCGGGTGGTCGGTGACCTCACCGATCCAAACTCGTTG
GCGCAAGCGGTCGAGGGCTGCGCGCACATCTTTCACGTGGCCGCCGATTATCGTCTCTACGTGCCCGATCCGACGGCGAT
GCGGGCGGTGAATATCGACGGCACCATTGCCCTGTTCCGCGCGGCACAGGCGGACGGGGCGTGCCGGATGGTCTATACCA
GCAGCGTCGCCGCTCTCGGCCTGACTCACGATGGCAGCCCGGCCGACGAGGCGACGCCGCATGCGGCGGAGGATCATGTC
GGCCCCTACAAGCGGTCGAAATACGAGGCCGAGTTGGCGGTGAAGAAGCTTGTCGCCGAGGGGTTGGATATCGTCATCGT
CAATCCGGCCGCCCCGGTTGGACCAGGCGACATCAAGCCGACGCCGACCGGCCGCATGGTGCTGGACGCCGCTCGCGGCC
ATATGCCAGCCTATGTCGAGACCGGCATGAACATCGTGCATGTGGATGACGTCGCCGAGGGTCATGTGCTTGCTCTGACG
CGGGGACGGCGCGGCGAGAGCTATATTCTCGGTGGCGAGAATCTCATGCTCTCCGAAATCGGGCGAATGATCACACGGCT
GGCCGGCAAGCCGCCGCCACGGGTAAAATTGCCGATCGGCCCGCTGATGCCGGTTGCGGCGCTCATGGAAGCCTGGGCGC
GGATCTCGGGCCATGAGCCGCTGATGACGCGCGACATGCTGACCATGGCGCGAAAGCGGATGTTCTATTCTTCGGAGAAG
GCCGTTCGGGAACTCGGCTATCATGCCCGCCCGGCAGAGGAAGCGATGCGCGATGCGCTCGCCGATTTCTGCCGCCGCGG
CCTACTGCCGAGCCTCGCCTTCGAGCCGGATGGAGCGACCGCATGA

Upstream 100 bases:

>100_bases
CCCTTAGCAGATCGCGCGGGTCCGGGGTATTGCCATCGCCGGGGGGCCTGCACTTTGCCTCGGCGCCGGCAATCAAGTAT
GCCCTCGGCAGGATTTTTCC

Downstream 100 bases:

>100_bases
CCCTGCTTGCCCTGCTCGCGCTGCTGGCTTGGATCTACCTATATCTGCTGCACGGCCAGTTCTGGCAGAGCGGCCCGGAA
CTCGCCCCGGCGCGGCCGGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MAGMSSDSSSPLETVARPIADDRPVLVTGATGFVGAAVARALVRHGFRLRLMHRASSDMRNLAQLPGERVVGDLTDPNSL
AQAVEGCAHIFHVAADYRLYVPDPTAMRAVNIDGTIALFRAAQADGACRMVYTSSVAALGLTHDGSPADEATPHAAEDHV
GPYKRSKYEAELAVKKLVAEGLDIVIVNPAAPVGPGDIKPTPTGRMVLDAARGHMPAYVETGMNIVHVDDVAEGHVLALT
RGRRGESYILGGENLMLSEIGRMITRLAGKPPPRVKLPIGPLMPVAALMEAWARISGHEPLMTRDMLTMARKRMFYSSEK
AVRELGYHARPAEEAMRDALADFCRRGLLPSLAFEPDGATA

Sequences:

>Translated_361_residues
MAGMSSDSSSPLETVARPIADDRPVLVTGATGFVGAAVARALVRHGFRLRLMHRASSDMRNLAQLPGERVVGDLTDPNSL
AQAVEGCAHIFHVAADYRLYVPDPTAMRAVNIDGTIALFRAAQADGACRMVYTSSVAALGLTHDGSPADEATPHAAEDHV
GPYKRSKYEAELAVKKLVAEGLDIVIVNPAAPVGPGDIKPTPTGRMVLDAARGHMPAYVETGMNIVHVDDVAEGHVLALT
RGRRGESYILGGENLMLSEIGRMITRLAGKPPPRVKLPIGPLMPVAALMEAWARISGHEPLMTRDMLTMARKRMFYSSEK
AVRELGYHARPAEEAMRDALADFCRRGLLPSLAFEPDGATA
>Mature_360_residues
AGMSSDSSSPLETVARPIADDRPVLVTGATGFVGAAVARALVRHGFRLRLMHRASSDMRNLAQLPGERVVGDLTDPNSLA
QAVEGCAHIFHVAADYRLYVPDPTAMRAVNIDGTIALFRAAQADGACRMVYTSSVAALGLTHDGSPADEATPHAAEDHVG
PYKRSKYEAELAVKKLVAEGLDIVIVNPAAPVGPGDIKPTPTGRMVLDAARGHMPAYVETGMNIVHVDDVAEGHVLALTR
GRRGESYILGGENLMLSEIGRMITRLAGKPPPRVKLPIGPLMPVAALMEAWARISGHEPLMTRDMLTMARKRMFYSSEKA
VRELGYHARPAEEAMRDALADFCRRGLLPSLAFEPDGATA

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI116268111, Length=371, Percent_Identity=25.3369272237197, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI239745448, Length=352, Percent_Identity=25.5681818181818, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI310132178, Length=352, Percent_Identity=25.5681818181818, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI310113012, Length=352, Percent_Identity=25.5681818181818, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71987463, Length=342, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 38626; Mature: 38495

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGMSSDSSSPLETVARPIADDRPVLVTGATGFVGAAVARALVRHGFRLRLMHRASSDMR
CCCCCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCCHHEEHHHHHHHHHH
NLAQLPGERVVGDLTDPNSLAQAVEGCAHIFHVAADYRLYVPDPTAMRAVNIDGTIALFR
HHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCCCEEEEE
AAQADGACRMVYTSSVAALGLTHDGSPADEATPHAAEDHVGPYKRSKYEAELAVKKLVAE
HHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHC
GLDIVIVNPAAPVGPGDIKPTPTGRMVLDAARGHMPAYVETGMNIVHVDDVAEGHVLALT
CCCEEEECCCCCCCCCCCCCCCCCCEEEEHHCCCCCHHHHCCCEEEEECCCCCCCEEEEE
RGRRGESYILGGENLMLSEIGRMITRLAGKPPPRVKLPIGPLMPVAALMEAWARISGHEP
CCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCC
LMTRDMLTMARKRMFYSSEKAVRELGYHARPAEEAMRDALADFCRRGLLPSLAFEPDGAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
A
C
>Mature Secondary Structure 
AGMSSDSSSPLETVARPIADDRPVLVTGATGFVGAAVARALVRHGFRLRLMHRASSDMR
CCCCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCCHHEEHHHHHHHHHH
NLAQLPGERVVGDLTDPNSLAQAVEGCAHIFHVAADYRLYVPDPTAMRAVNIDGTIALFR
HHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCCCEEEEE
AAQADGACRMVYTSSVAALGLTHDGSPADEATPHAAEDHVGPYKRSKYEAELAVKKLVAE
HHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHC
GLDIVIVNPAAPVGPGDIKPTPTGRMVLDAARGHMPAYVETGMNIVHVDDVAEGHVLALT
CCCEEEECCCCCCCCCCCCCCCCCCEEEEHHCCCCCHHHHCCCEEEEECCCCCCCEEEEE
RGRRGESYILGGENLMLSEIGRMITRLAGKPPPRVKLPIGPLMPVAALMEAWARISGHEP
CCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCC
LMTRDMLTMARKRMFYSSEKAVRELGYHARPAEEAMRDALADFCRRGLLPSLAFEPDGAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]