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The map label for this gene is pncA [H]

Identifier: 148259106

GI number: 148259106

Start: 93545

End: 94156

Strand: Reverse

Name: pncA [H]

Synonym: Acry_0085

Alternate gene names: 148259106

Gene position: 94156-93545 (Counterclockwise)

Preceding gene: 148259109

Following gene: 148259105

Centisome position: 2.78

GC content: 65.52

Gene sequence:

>612_bases
ATGGACAGCCGCACCGCGCTTCTCGTCATCGATGTCCAGACAGATTTTTGCGCCGGAGGTGCCCTTGCCGTACCGAATGG
CGACGTGGTGGTGCCGGTGATCAACGCTCTTGCGCGGCACTACCAGACGGTCGTCATCACGCAGGACTGGCACCCGGCCG
ACCATGTCTCCTTCGCCAGCCAGCATCCGGGACGGTCTCCGTTCGAAACCATACCCCTCGTCTACGGAACGCAGGTGCTC
TGGCCGGATCACTGCGTGATGGACAGCCCCGGCGCAGCGCTGCACCGCGACCTCCATATTCCCCATGCCGCAGTGATCCA
GCGCAAGGGATTGAATCGCCTGATCGATTCCTATTCCGCCTTCCTGGAAGCCGATCGGACAACCCGCACGGGTCTCGACG
GCTGGCTCGCCGCCCGCGGCATCGAGCGGGTCGATCTCTGCGGCCTCGCGACTGACTATTGTGTCGCCTGGTCGGCACAG
GATGCGAGGCGCTTCGGTCTGGAAGCACGTGTGATCGAGCCGGCCTGCCGGGGCATCGATCTGGATGGATCGCTGGCGGC
AGCGTGGCAGGCGATGACCGCGGCAGGCGTGGCGCGGATCGCCGCGGTCTGA

Upstream 100 bases:

>100_bases
CCGCGTCGCAGGCAACAACTGTGATTATTACCGATAACGGACATCTTCGGCAACCGTCCGCCCCCTATCTCGCCGGTCCG
ATGCGGACTATCTTCACGAT

Downstream 100 bases:

>100_bases
TACCAGCGGCTTCATGACCGGCGACGGCGAGCAGCAGCGGACTAGGCAGAGGGCCTCGCGCAGGCGTGCCACTCTCATTG
CCGGTTTGCTGGGTCTTGCC

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MDSRTALLVIDVQTDFCAGGALAVPNGDVVVPVINALARHYQTVVITQDWHPADHVSFASQHPGRSPFETIPLVYGTQVL
WPDHCVMDSPGAALHRDLHIPHAAVIQRKGLNRLIDSYSAFLEADRTTRTGLDGWLAARGIERVDLCGLATDYCVAWSAQ
DARRFGLEARVIEPACRGIDLDGSLAAAWQAMTAAGVARIAAV

Sequences:

>Translated_203_residues
MDSRTALLVIDVQTDFCAGGALAVPNGDVVVPVINALARHYQTVVITQDWHPADHVSFASQHPGRSPFETIPLVYGTQVL
WPDHCVMDSPGAALHRDLHIPHAAVIQRKGLNRLIDSYSAFLEADRTTRTGLDGWLAARGIERVDLCGLATDYCVAWSAQ
DARRFGLEARVIEPACRGIDLDGSLAAAWQAMTAAGVARIAAV
>Mature_203_residues
MDSRTALLVIDVQTDFCAGGALAVPNGDVVVPVINALARHYQTVVITQDWHPADHVSFASQHPGRSPFETIPLVYGTQVL
WPDHCVMDSPGAALHRDLHIPHAAVIQRKGLNRLIDSYSAFLEADRTTRTGLDGWLAARGIERVDLCGLATDYCVAWSAQ
DARRFGLEARVIEPACRGIDLDGSLAAAWQAMTAAGVARIAAV

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=196, Percent_Identity=47.9591836734694, Blast_Score=169, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI193206735, Length=223, Percent_Identity=31.8385650224215, Blast_Score=103, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17543130, Length=223, Percent_Identity=31.8385650224215, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71999339, Length=221, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321401, Length=193, Percent_Identity=31.6062176165803, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648247, Length=217, Percent_Identity=34.5622119815668, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 21876; Mature: 21876

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSRTALLVIDVQTDFCAGGALAVPNGDVVVPVINALARHYQTVVITQDWHPADHVSFAS
CCCCEEEEEEECCCCCCCCCEEECCCCCEEHHHHHHHHHHHEEEEEEECCCCCHHCCHHH
QHPGRSPFETIPLVYGTQVLWPDHCVMDSPGAALHRDLHIPHAAVIQRKGLNRLIDSYSA
CCCCCCCCCCCCHHHCCEEECCCCCEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FLEADRTTRTGLDGWLAARGIERVDLCGLATDYCVAWSAQDARRFGLEARVIEPACRGID
HHHCCCCCCCCCHHHHHHCCCCHHHHHCCCHHHHEEECCCHHHHHCCEEEEECHHHCCCC
LDGSLAAAWQAMTAAGVARIAAV
CCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSRTALLVIDVQTDFCAGGALAVPNGDVVVPVINALARHYQTVVITQDWHPADHVSFAS
CCCCEEEEEEECCCCCCCCCEEECCCCCEEHHHHHHHHHHHEEEEEEECCCCCHHCCHHH
QHPGRSPFETIPLVYGTQVLWPDHCVMDSPGAALHRDLHIPHAAVIQRKGLNRLIDSYSA
CCCCCCCCCCCCHHHCCEEECCCCCEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FLEADRTTRTGLDGWLAARGIERVDLCGLATDYCVAWSAQDARRFGLEARVIEPACRGID
HHHCCCCCCCCCHHHHHHCCCCHHHHHCCCHHHHEEECCCHHHHHCCEEEEECHHHCCCC
LDGSLAAAWQAMTAAGVARIAAV
CCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]