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The map label for this gene is rlmB [H]

Identifier: 148259103

GI number: 148259103

Start: 88570

End: 89400

Strand: Reverse

Name: rlmB [H]

Synonym: Acry_0082

Alternate gene names: 148259103

Gene position: 89400-88570 (Counterclockwise)

Preceding gene: 148259105

Following gene: 148259095

Centisome position: 2.64

GC content: 67.15

Gene sequence:

>831_bases
ATGGACAAGCGCGACTCTCACGGAAAGGCCGGCAACGGCGGCCGCCACAAACTTCCTCGCGGCCCGCATCCTCACAGGCG
GCCACAAGGCGATGCGCCGCGTGACCGGCGACCTGCATCATCCGCCGGTGGGCTTTGGCTCTATGGATTGCATTCCGTCG
AGGCGGCGCTTGCGAATCCCGCCCGCAGGCTGCGCCGCCTCGTTGTTACCGAGGAAGGCGAAGCCGCACTCGCCGGGCGC
CTGCCGCATCCATGGCCGATTCAACCCGAGCGGATCGAGCGCGATCGACTCGACCAGATGCTTGGCCGCGGTATCGTGCA
TCAGGGCGTTGCCCTGCAGGCCGATCCCCTGGTGCCGCCATCGCTGAACGACGCACTCAAACGTCCGGGCCCGGTTCTCG
TGCTCGATCAGATCACCGACCCGCGCAATGTCGGGGCGATCCTGCGCTCAGCCCAGGCCTTCGGCGCGGCAATGGTCATT
GCGCAGGAACGCAATGCCCCAGAGGAGGGCGGTGCACTCGCCAAGGCAGCTTCCGGCGCGCTGGAACGGATACCGCTGCT
ACGCACAGTGAACATCGCTCGGGCGCTGATCGCGCTAAAGGCGGCGGGTCTGTGGGTCATGGGGCTCGATGCGGCAGGAT
TGTCGCATCTGTCGCGCGACGCGATCGGCAACCGTCGTGCCGCCCTGGTGCTCGGGGCCGAGGGCGAAGGGCTGCGCCGC
CTGACCCGCGAAACCTGCGACGAACTTGTCTCGCTGCCGATGCCGGGCGGAATGGAAAGCCTTAATGTGTCAGCAGCCGC
GGCCGTGGCGCTCTACGAAATCATCCGGTAG

Upstream 100 bases:

>100_bases
CAGTCCGCCCCCTTTAGCCACTCGGGCACCTCTCCGCCGCCCGTAATCTTCGATCGCGCCGGCGTTGTCAACATCGGTGG
TCGCAGGCTAGAGAGCCGCC

Downstream 100 bases:

>100_bases
GCGAAATCCTCGGCACCCGCTGCGCTTTCCGCTACAGCGAGTTCCTTCATCATCGCATGATGCGGCGAGAGGCCGCAGGC
AGGGTCCGTTGCCGCGGCAT

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MDKRDSHGKAGNGGRHKLPRGPHPHRRPQGDAPRDRRPASSAGGLWLYGLHSVEAALANPARRLRRLVVTEEGEAALAGR
LPHPWPIQPERIERDRLDQMLGRGIVHQGVALQADPLVPPSLNDALKRPGPVLVLDQITDPRNVGAILRSAQAFGAAMVI
AQERNAPEEGGALAKAASGALERIPLLRTVNIARALIALKAAGLWVMGLDAAGLSHLSRDAIGNRRAALVLGAEGEGLRR
LTRETCDELVSLPMPGGMESLNVSAAAAVALYEIIR

Sequences:

>Translated_276_residues
MDKRDSHGKAGNGGRHKLPRGPHPHRRPQGDAPRDRRPASSAGGLWLYGLHSVEAALANPARRLRRLVVTEEGEAALAGR
LPHPWPIQPERIERDRLDQMLGRGIVHQGVALQADPLVPPSLNDALKRPGPVLVLDQITDPRNVGAILRSAQAFGAAMVI
AQERNAPEEGGALAKAASGALERIPLLRTVNIARALIALKAAGLWVMGLDAAGLSHLSRDAIGNRRAALVLGAEGEGLRR
LTRETCDELVSLPMPGGMESLNVSAAAAVALYEIIR
>Mature_276_residues
MDKRDSHGKAGNGGRHKLPRGPHPHRRPQGDAPRDRRPASSAGGLWLYGLHSVEAALANPARRLRRLVVTEEGEAALAGR
LPHPWPIQPERIERDRLDQMLGRGIVHQGVALQADPLVPPSLNDALKRPGPVLVLDQITDPRNVGAILRSAQAFGAAMVI
AQERNAPEEGGALAKAASGALERIPLLRTVNIARALIALKAAGLWVMGLDAAGLSHLSRDAIGNRRAALVLGAEGEGLRR
LTRETCDELVSLPMPGGMESLNVSAAAAVALYEIIR

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=255, Percent_Identity=32.5490196078431, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1790623, Length=239, Percent_Identity=38.0753138075314, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1790083, Length=158, Percent_Identity=30.379746835443, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=252, Percent_Identity=28.968253968254, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29396; Mature: 29396

Theoretical pI: Translated: 10.93; Mature: 10.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKRDSHGKAGNGGRHKLPRGPHPHRRPQGDAPRDRRPASSAGGLWLYGLHSVEAALANP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHH
ARRLRRLVVTEEGEAALAGRLPHPWPIQPERIERDRLDQMLGRGIVHQGVALQADPLVPP
HHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
SLNDALKRPGPVLVLDQITDPRNVGAILRSAQAFGAAMVIAQERNAPEEGGALAKAASGA
CCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHCCHHHHHHHHH
LERIPLLRTVNIARALIALKAAGLWVMGLDAAGLSHLSRDAIGNRRAALVLGAEGEGLRR
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
LTRETCDELVSLPMPGGMESLNVSAAAAVALYEIIR
HHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHC
>Mature Secondary Structure
MDKRDSHGKAGNGGRHKLPRGPHPHRRPQGDAPRDRRPASSAGGLWLYGLHSVEAALANP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHH
ARRLRRLVVTEEGEAALAGRLPHPWPIQPERIERDRLDQMLGRGIVHQGVALQADPLVPP
HHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
SLNDALKRPGPVLVLDQITDPRNVGAILRSAQAFGAAMVIAQERNAPEEGGALAKAASGA
CCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHCCHHHHHHHHH
LERIPLLRTVNIARALIALKAAGLWVMGLDAAGLSHLSRDAIGNRRAALVLGAEGEGLRR
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
LTRETCDELVSLPMPGGMESLNVSAAAAVALYEIIR
HHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]