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The map label for this gene is wcaJ [H]

Identifier: 148259094

GI number: 148259094

Start: 79236

End: 80720

Strand: Reverse

Name: wcaJ [H]

Synonym: Acry_0073

Alternate gene names: 148259094

Gene position: 80720-79236 (Counterclockwise)

Preceding gene: 148259095

Following gene: 148259093

Centisome position: 2.38

GC content: 68.48

Gene sequence:

>1485_bases
ATGTCTCAGACGATTCTAGAGCCTTTGGCCGAACCAGTCCGCCGTATGGCGGCAATCCCGGTCAAGCGGGCGCACCGATT
CCGTCCGCACCTCGGCCCGAGTAGCGGAATCGCATTTCTCCTGGTCACGGATGCCGCGACCGTTCTCATTGGCCCGCGTC
TTGCCACCCGAGTGTTGGCGCCTGCCCCCACCATGGCCGCCGGGCCGTTCAACGAACTCGTCTTTGGTGGCCTGACATTG
CTCGCCCTGTTCGCTGCCGGTCTTTACGCAGTCGGCCCCGCGCGCGCGCCGCTCGTCCATATCGGAGCCATGCTGCGCGC
CCTCGTGGCGGCGGACGTGCTTGCCCTGCTGCGGATCGCACTGTTGCGCCCTGTGCCAGCCATATTGCCGCTCGACAGAT
CAATTGCATGGGCCGTCGGTGCCTTCACGTTCACTGCCGTGCTGATGCTCGGCGAGCGCGCAATGGTTGCCGCGTTCTGG
CGCCGCCACGGAATCGGCATCCCGCGAGGAGCATCCGCCCTCGTTGCCGCAACTCCGTGCGACCGTATGCTCTGCACTCG
TCTCGACCGCATGACCGACCACCGGCTTGGCTATGTCTTCTGCTGGGGGCTCGCCCCGACATCAGGCCTGCGCACCATCG
CCGATGCGGCGACCCTGGAAGCCATGATCCGCTCTGGCCAGATCGGAGATCTCGTCGTCTTCGTCCAGCGCGGCGATGGG
CCCGAAGCCCGCAGCAAAATCGATGCACTGCTTGCCCGCCTTGCCGATCAGCCGGTGCGGATCCGCCTCGCCTTCGACGC
GGTGGCCGAGATCGGCGCGTGCGACCTCGCGACCGGCCGGAGCCTGCGTCTCGTCACGGTGCTCGACCGGCCGATCCGTC
CGTTCGGCGCCGTGGCAAAGCGGAGCATGGACGTGGGCGGCGCCGCCTTGCTGATCATCCTGCTCGGCCCGCTTCTGGCC
CTGATCGCCCTCGCCCTACGGATGACCGGACCCGTCCTGTTCCGCCAGCGCCGCATCGGGCTCGACGGCGCGCCGTTCAC
GGTCTTCAAGTTCCGCACGATGCGCCCGCAGCCACCCAGACAAGCCGCAGGCCCGGTGTCTCAGGCCCAGCCTGGCGATC
GGCGGATCACCCGGCTCGGCGCCATTCTCCGCCGCCTTTCGCTCGACGAATTACCCCAACTATTCAACGTGCTGCGCGGC
GAGATGTCGCTGGTCGGCCCCCGCCCGCACGCGCCGCACACCACCGCCGGCGACTTCACCTTCGAACAGGCGATCGCCTT
CTACGGCGTGCGCCACCGCGTGAAGCCGGGGTTGACCGGCCTCGCCCAGGTGCGCGGCCTGCGCGGCCCGACCGACCGGC
GCGACCTCGTGGCGGCCCGAGTCGCCGCCGATCTCGACTATATCGAGCGCTGGTCCCCCTGGCTCGACCTCGCCATCCTG
TTGCGCACGATCCCCGCTGTGCTGATCGGCCGCAATGCCATCTGA

Upstream 100 bases:

>100_bases
GCTTCCTCATCAATTTTCTGCTTTCCCGCCGCCTCGTTTTCGGTTAACCAAAAATCAACCTTGGATTGCGGTCAAGCAAC
TCTAGGTGGAGGCGAACCGG

Downstream 100 bases:

>100_bases
GCCGCGCCTGCTGGCCGGGACGCCGCCCCCTGGCCACGCCTATGATGCCGACATTGTCATCCTGAGCCATCACCGCCCGC
GTGAAACCATCGCCGCGATC

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSQTILEPLAEPVRRMAAIPVKRAHRFRPHLGPSSGIAFLLVTDAATVLIGPRLATRVLAPAPTMAAGPFNELVFGGLTL
LALFAAGLYAVGPARAPLVHIGAMLRALVAADVLALLRIALLRPVPAILPLDRSIAWAVGAFTFTAVLMLGERAMVAAFW
RRHGIGIPRGASALVAATPCDRMLCTRLDRMTDHRLGYVFCWGLAPTSGLRTIADAATLEAMIRSGQIGDLVVFVQRGDG
PEARSKIDALLARLADQPVRIRLAFDAVAEIGACDLATGRSLRLVTVLDRPIRPFGAVAKRSMDVGGAALLIILLGPLLA
LIALALRMTGPVLFRQRRIGLDGAPFTVFKFRTMRPQPPRQAAGPVSQAQPGDRRITRLGAILRRLSLDELPQLFNVLRG
EMSLVGPRPHAPHTTAGDFTFEQAIAFYGVRHRVKPGLTGLAQVRGLRGPTDRRDLVAARVAADLDYIERWSPWLDLAIL
LRTIPAVLIGRNAI

Sequences:

>Translated_494_residues
MSQTILEPLAEPVRRMAAIPVKRAHRFRPHLGPSSGIAFLLVTDAATVLIGPRLATRVLAPAPTMAAGPFNELVFGGLTL
LALFAAGLYAVGPARAPLVHIGAMLRALVAADVLALLRIALLRPVPAILPLDRSIAWAVGAFTFTAVLMLGERAMVAAFW
RRHGIGIPRGASALVAATPCDRMLCTRLDRMTDHRLGYVFCWGLAPTSGLRTIADAATLEAMIRSGQIGDLVVFVQRGDG
PEARSKIDALLARLADQPVRIRLAFDAVAEIGACDLATGRSLRLVTVLDRPIRPFGAVAKRSMDVGGAALLIILLGPLLA
LIALALRMTGPVLFRQRRIGLDGAPFTVFKFRTMRPQPPRQAAGPVSQAQPGDRRITRLGAILRRLSLDELPQLFNVLRG
EMSLVGPRPHAPHTTAGDFTFEQAIAFYGVRHRVKPGLTGLAQVRGLRGPTDRRDLVAARVAADLDYIERWSPWLDLAIL
LRTIPAVLIGRNAI
>Mature_493_residues
SQTILEPLAEPVRRMAAIPVKRAHRFRPHLGPSSGIAFLLVTDAATVLIGPRLATRVLAPAPTMAAGPFNELVFGGLTLL
ALFAAGLYAVGPARAPLVHIGAMLRALVAADVLALLRIALLRPVPAILPLDRSIAWAVGAFTFTAVLMLGERAMVAAFWR
RHGIGIPRGASALVAATPCDRMLCTRLDRMTDHRLGYVFCWGLAPTSGLRTIADAATLEAMIRSGQIGDLVVFVQRGDGP
EARSKIDALLARLADQPVRIRLAFDAVAEIGACDLATGRSLRLVTVLDRPIRPFGAVAKRSMDVGGAALLIILLGPLLAL
IALALRMTGPVLFRQRRIGLDGAPFTVFKFRTMRPQPPRQAAGPVSQAQPGDRRITRLGAILRRLSLDELPQLFNVLRGE
MSLVGPRPHAPHTTAGDFTFEQAIAFYGVRHRVKPGLTGLAQVRGLRGPTDRRDLVAARVAADLDYIERWSPWLDLAILL
RTIPAVLIGRNAI

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=284, Percent_Identity=38.0281690140845, Blast_Score=192, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53193; Mature: 53062

Theoretical pI: Translated: 11.95; Mature: 11.95

Prosite motif: PS00888 CNMP_BINDING_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTILEPLAEPVRRMAAIPVKRAHRFRPHLGPSSGIAFLLVTDAATVLIGPRLATRVLA
CCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEECCHHHHCCHHHHHHHHC
PAPTMAAGPFNELVFGGLTLLALFAAGLYAVGPARAPLVHIGAMLRALVAADVLALLRIA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLRPVPAILPLDRSIAWAVGAFTFTAVLMLGERAMVAAFWRRHGIGIPRGASALVAATPC
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCH
DRMLCTRLDRMTDHRLGYVFCWGLAPTSGLRTIADAATLEAMIRSGQIGDLVVFVQRGDG
HHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
PEARSKIDALLARLADQPVRIRLAFDAVAEIGACDLATGRSLRLVTVLDRPIRPFGAVAK
HHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
RSMDVGGAALLIILLGPLLALIALALRMTGPVLFRQRRIGLDGAPFTVFKFRTMRPQPPR
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEEEEECCCCCCCH
QAAGPVSQAQPGDRRITRLGAILRRLSLDELPQLFNVLRGEMSLVGPRPHAPHTTAGDFT
HHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCHH
FEQAIAFYGVRHRVKPGLTGLAQVRGLRGPTDRRDLVAARVAADLDYIERWSPWLDLAIL
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LRTIPAVLIGRNAI
HHHHHHHHHCCCCC
>Mature Secondary Structure 
SQTILEPLAEPVRRMAAIPVKRAHRFRPHLGPSSGIAFLLVTDAATVLIGPRLATRVLA
CHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEECCHHHHCCHHHHHHHHC
PAPTMAAGPFNELVFGGLTLLALFAAGLYAVGPARAPLVHIGAMLRALVAADVLALLRIA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLRPVPAILPLDRSIAWAVGAFTFTAVLMLGERAMVAAFWRRHGIGIPRGASALVAATPC
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCH
DRMLCTRLDRMTDHRLGYVFCWGLAPTSGLRTIADAATLEAMIRSGQIGDLVVFVQRGDG
HHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
PEARSKIDALLARLADQPVRIRLAFDAVAEIGACDLATGRSLRLVTVLDRPIRPFGAVAK
HHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
RSMDVGGAALLIILLGPLLALIALALRMTGPVLFRQRRIGLDGAPFTVFKFRTMRPQPPR
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEEEEECCCCCCCH
QAAGPVSQAQPGDRRITRLGAILRRLSLDELPQLFNVLRGEMSLVGPRPHAPHTTAGDFT
HHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCHH
FEQAIAFYGVRHRVKPGLTGLAQVRGLRGPTDRRDLVAARVAADLDYIERWSPWLDLAIL
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LRTIPAVLIGRNAI
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]