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The map label for this gene is aroA

Identifier: 148259052

GI number: 148259052

Start: 28947

End: 30284

Strand: Reverse

Name: aroA

Synonym: Acry_0031

Alternate gene names: 148259052

Gene position: 30284-28947 (Counterclockwise)

Preceding gene: 148259054

Following gene: 148259051

Centisome position: 0.89

GC content: 71.97

Gene sequence:

>1338_bases
ATGGAACACGCCGCAACGCTGCCCCAGACCTCACGCCGCCCTGCCACGCCGCTCACCGGCACGATCACCGTTCCGGGCGA
CAAGTCGATTTCGCATCGCGCGCTGATGTTCGCCGGCCTCGCGGTCGGTGAAACCCGGATTTCCGGCCTGCTGGAAGGCG
AGGACGTGCTGCGGACCGCCGCCGCGATGCGCGCGCTGGGCGCGACGGTGGAGCGTACCGGCCCGGGTGCCTGGCGCGCC
TCCGGCCCCGGCATCGGCGGGTTGCAGAGCCCGGACGACGTGCTCGACATGGGCAATTCCGGCACCGCCGCGCGGCTGCT
CTGCGGCATTCTCGCGAGCCACGACATTTTCGCCGTGATGACCGGCGATGCCTCGCTCCGCCGCCGGCCGATGCGCCGGG
TGATCGATCCGCTGGGCGCGACAGGCGCCGTCTTCGCCGCGCGAGAGGGCGGGCGCCTGCCGCTTTCGGTGCGCGGTGCC
GCCGAGGCGATGCCGCTCTCCTATCGGCTGCCGGTCGCCTCCGCGCAGGTGAAGTCGGCGCTGCTGCTCGCCGGGCTGAA
CGCCCGCGGCATCACCGAGATCGAGGAACCGGAGCCGACCCGCGACCATTCCGAGAACATGCTCCGCCATTTCGGCGCCG
AGGTGGAGGTGGCCGCGCATGGCACGGGCAAGCTGATCCGCCTGCGGGGACAGCCGGAGCTGCGCGGCGCGGATGTCGTC
GTGCCGGGCGATCCGTCGTCGGCCGCCTTCCCGATCGTCGCCGCGCTGATCGTGCCGGGCTCGCGCGTCACGATCGGGGG
CGTCGGGCTGAACCCGCTGCGGACCGGGCTGTTCCTCACCCTGCGCGAGATGGGAGCAGCGATCGAGATCGTAAATGCCC
GCGAGGCCGGCGGCGAGCCCGTGGGCGATCTCATCGTCACCGCCTCGGATCTGCGCGCCGTCGATGTGCCGGCGGAACGC
GCCCCCTCGATGATCGACGAATACCCGATTCTCGCCGTCGCCTGCGCGATGGCGCGCGGCAGCTCGCGGCTGCGTGGCCT
CGCGGAGCTGCGGGTGAAGGAAAGCGACCGGCTGGCCGCGACGCTCGCCATGATCACCGCCAATGGCGTCAAGGCGCAGG
TGGACGGCGACGACCTGATCATCGAGGGCGGCGGCGCGCGCGGCGGCGCCACGGTGGCGACGCATATGGATCACCGCCTG
GCAATGAGCGCGCTCGTCATGGGCCTCGCGACCGAGACCCCGGTGACCATCGACGATGCGCGCTTTATCGATACGAGCTT
CCCCGGCTTCCTGCCGCTGATGCGCGGAATCGGCGCGGCGATCGAGGCGGCGGCGTGA

Upstream 100 bases:

>100_bases
AGGGCGGGCGCCATGCCACCCATCGCCCTGCTTGTCAAACCCTGACGGCCGCCGACAATGATGGCTGGCAAAATCGTCCA
CTCGTGGTAAGCGCCGCGCC

Downstream 100 bases:

>100_bases
CGCGGCCGATCGTCATCGCGATCGACGGGCCGGCCGCCTCCGGCAAGGGCACGCTCGCGCGCCGCCTCGCCGCCGCCTTC
AACCTGCCCTATCTCGATAC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MEHAATLPQTSRRPATPLTGTITVPGDKSISHRALMFAGLAVGETRISGLLEGEDVLRTAAAMRALGATVERTGPGAWRA
SGPGIGGLQSPDDVLDMGNSGTAARLLCGILASHDIFAVMTGDASLRRRPMRRVIDPLGATGAVFAAREGGRLPLSVRGA
AEAMPLSYRLPVASAQVKSALLLAGLNARGITEIEEPEPTRDHSENMLRHFGAEVEVAAHGTGKLIRLRGQPELRGADVV
VPGDPSSAAFPIVAALIVPGSRVTIGGVGLNPLRTGLFLTLREMGAAIEIVNAREAGGEPVGDLIVTASDLRAVDVPAER
APSMIDEYPILAVACAMARGSSRLRGLAELRVKESDRLAATLAMITANGVKAQVDGDDLIIEGGGARGGATVATHMDHRL
AMSALVMGLATETPVTIDDARFIDTSFPGFLPLMRGIGAAIEAAA

Sequences:

>Translated_445_residues
MEHAATLPQTSRRPATPLTGTITVPGDKSISHRALMFAGLAVGETRISGLLEGEDVLRTAAAMRALGATVERTGPGAWRA
SGPGIGGLQSPDDVLDMGNSGTAARLLCGILASHDIFAVMTGDASLRRRPMRRVIDPLGATGAVFAAREGGRLPLSVRGA
AEAMPLSYRLPVASAQVKSALLLAGLNARGITEIEEPEPTRDHSENMLRHFGAEVEVAAHGTGKLIRLRGQPELRGADVV
VPGDPSSAAFPIVAALIVPGSRVTIGGVGLNPLRTGLFLTLREMGAAIEIVNAREAGGEPVGDLIVTASDLRAVDVPAER
APSMIDEYPILAVACAMARGSSRLRGLAELRVKESDRLAATLAMITANGVKAQVDGDDLIIEGGGARGGATVATHMDHRL
AMSALVMGLATETPVTIDDARFIDTSFPGFLPLMRGIGAAIEAAA
>Mature_445_residues
MEHAATLPQTSRRPATPLTGTITVPGDKSISHRALMFAGLAVGETRISGLLEGEDVLRTAAAMRALGATVERTGPGAWRA
SGPGIGGLQSPDDVLDMGNSGTAARLLCGILASHDIFAVMTGDASLRRRPMRRVIDPLGATGAVFAAREGGRLPLSVRGA
AEAMPLSYRLPVASAQVKSALLLAGLNARGITEIEEPEPTRDHSENMLRHFGAEVEVAAHGTGKLIRLRGQPELRGADVV
VPGDPSSAAFPIVAALIVPGSRVTIGGVGLNPLRTGLFLTLREMGAAIEIVNAREAGGEPVGDLIVTASDLRAVDVPAER
APSMIDEYPILAVACAMARGSSRLRGLAELRVKESDRLAATLAMITANGVKAQVDGDDLIIEGGGARGGATVATHMDHRL
AMSALVMGLATETPVTIDDARFIDTSFPGFLPLMRGIGAAIEAAA

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=446, Percent_Identity=30.2690582959641, Blast_Score=129, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320332, Length=472, Percent_Identity=27.1186440677966, Blast_Score=113, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_ACICJ (A5FUH8)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001233179.1
- ProteinModelPortal:   A5FUH8
- SMR:   A5FUH8
- STRING:   A5FUH8
- GeneID:   5161081
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_0031
- eggNOG:   COG0128
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BioCyc:   ACRY349163:ACRY_0031-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 45983; Mature: 45983

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHAATLPQTSRRPATPLTGTITVPGDKSISHRALMFAGLAVGETRISGLLEGEDVLRTA
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHHHCCCHHHHHHHH
AAMRALGATVERTGPGAWRASGPGIGGLQSPDDVLDMGNSGTAARLLCGILASHDIFAVM
HHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCEEEEE
TGDASLRRRPMRRVIDPLGATGAVFAAREGGRLPLSVRGAAEAMPLSYRLPVASAQVKSA
ECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCEEECCCCHHHHHHH
LLLAGLNARGITEIEEPEPTRDHSENMLRHFGAEVEVAAHGTGKLIRLRGQPELRGADVV
HHEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCEEE
VPGDPSSAAFPIVAALIVPGSRVTIGGVGLNPLRTGLFLTLREMGAAIEIVNAREAGGEP
ECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCC
VGDLIVTASDLRAVDVPAERAPSMIDEYPILAVACAMARGSSRLRGLAELRVKESDRLAA
CCCEEEEECCCEEECCCHHHCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHH
TLAMITANGVKAQVDGDDLIIEGGGARGGATVATHMDHRLAMSALVMGLATETPVTIDDA
EEEEEECCCEEEEECCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEECCC
RFIDTSFPGFLPLMRGIGAAIEAAA
EEECCCCCHHHHHHHHCCHHHHCCC
>Mature Secondary Structure
MEHAATLPQTSRRPATPLTGTITVPGDKSISHRALMFAGLAVGETRISGLLEGEDVLRTA
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHHHCCCHHHHHHHH
AAMRALGATVERTGPGAWRASGPGIGGLQSPDDVLDMGNSGTAARLLCGILASHDIFAVM
HHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCEEEEE
TGDASLRRRPMRRVIDPLGATGAVFAAREGGRLPLSVRGAAEAMPLSYRLPVASAQVKSA
ECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCEEECCCCHHHHHHH
LLLAGLNARGITEIEEPEPTRDHSENMLRHFGAEVEVAAHGTGKLIRLRGQPELRGADVV
HHEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCEEE
VPGDPSSAAFPIVAALIVPGSRVTIGGVGLNPLRTGLFLTLREMGAAIEIVNAREAGGEP
ECCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCC
VGDLIVTASDLRAVDVPAERAPSMIDEYPILAVACAMARGSSRLRGLAELRVKESDRLAA
CCCEEEEECCCEEECCCHHHCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHH
TLAMITANGVKAQVDGDDLIIEGGGARGGATVATHMDHRLAMSALVMGLATETPVTIDDA
EEEEEECCCEEEEECCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEECCC
RFIDTSFPGFLPLMRGIGAAIEAAA
EEECCCCCHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA