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The map label for this gene is spoT [H]

Identifier: 148243334

GI number: 148243334

Start: 1941800

End: 1944121

Strand: Direct

Name: spoT [H]

Synonym: SynRCC307_2235

Alternate gene names: 148243334

Gene position: 1941800-1944121 (Clockwise)

Preceding gene: 148243331

Following gene: 148243335

Centisome position: 87.28

GC content: 62.32

Gene sequence:

>2322_bases
ATGCTGGACGCCGCCGAACGGTTTGCCACCTCAACAGCGTCAACGCTGCCTGGTCGAGCCGAGTGCGATGCGCCCCAACT
CAGGCAAGAGATCGCTGGGCCCGATGACTACGCCATTGATCTGCCCGCATGGCTCAGGCAGTGCATCGAGCATGTGCCCC
CTGGCCAGGGCGAAACCTGCCCCACCGACCCAGAGGGTCTGCTGGCATCGGCCTTTGATTTCGCCTATCAACTCCACCGC
CAGCAGGTGCGAGCCACCGGTGAGCCCTACATCATCCATCCGGTGGCCGTGGCGGATTTGCTGCGCGACATCGGCGCCAG
CGCTGCGGTGATCGCGGCTGGGTTCCTCCACGACGTGGTGGAAGACACCGATGTGAGCCTGGAAGAAATCGAAGGGCACT
TCGGAGCCGAAGTGCGGCAACTGGTGGAGGGCGTCACCAAGCTGGGCGGCATCCACTTCACCAACCGAACCGAGGCCCAA
GCTGAAAACCTGCGCAAGATGTTTTTGGCCATGGCCAGCGACATCCGCGTGGTGTTGGTGAAGCTGGCCGACCGGCTGCA
CAACATGCGCACCCTGGGTGCACTGCGCCCTGAGAAGCAGCAGCGCATCGCCACCGAAACCCGCGACATCTACGCGCCCC
TGGCCAACCGCCTTGGCATTGGCCGGTTCAAGTGGGAGCTGGAGGATCTGGCCTTCAAGGTGCTGGAACCCGATGACTTC
CGTTCCATCTCCCAGCAGGTGGCCAGCAAACGCAGCGACCGGGAAGAACGCTTAGGCGTCACCGTTCAGCTGCTGCGCGA
TCGGCTGGAGAACTCAGGCGTGCATGAGTGCGAGGTGGTGGGGCGACCCAAACACCTCTATGGCATCTGGAGCAAGATGC
AGCGCCAGGAGAAGGAGTTCCACGAAATCTTTGATGTGGCGGCGGTGCGGATCGTGGCTCCATCGGTGGAAACCTGCTAC
CGCGCCCTCGCCGTGGTGCATGACACCTTCCGGCCCATCCCAGGTCGCTTTAAGGACTACATCGGCCTGCCCAAACCCAA
CGGCTACCAATCGCTGCACACCGCTGTGATTGGTCGGCATCGGCCGATCGAAGTGCAGATCCGCACCGGCGAGATGCATG
AAGTGGCCGAGTACGGCATTGCCGCCCACTGGAAATACAAAGAGGGTGGCTCCCCCGCTGATGGGGATGAAATGGCCCGC
TTCAACTGGCTACGGCAGCTGGTTGATTGGCAAAAAGAAGAAGGGGGACAAGACCACAACGACTACTTGCGCTCGATCAA
AGAAGACCTCTTTGACGACGAGGTGTTTGTGTTCACCCCCAATGGCGATGTGATCGGCCTGCGCAAAGGGTCCACTGCCG
TGGACTTTGCCTACCGCATTCACAGCGAGGTGGGGAACCACTGCCAGGGCGTACGCATCAACGATCGGCTCTGCCCGCTT
GCCACCCTCCTACAGAACGGCGACTTCGTTCAGATCGTGACGGCCAAAACCGCTCACCCCAGCCTGGACTGGCTGAATTT
CGTCGCCACACCAACGGCGCGCGAGCGCATCCGCCAGTGGTACAAGCGCAGTCACCGCGACGACAACATCACACGCGGCC
GCGAAATGCTGGAGCGGGAGCTGGGCCGTGATGGCTTCGATGCCCTGCTCAGCAGCGACCCAATCCTGCGCGTCGCCCGC
CGCTGCAACCTCACCGGTGTCGATGACCTGCTGGCCTCCCTGGGTTTTGGCGGCGTCACGGTGCATCAAGTCACCAATCG
TCTGCGCGAGGAGATGCGGCTGGAGGAGGAATCCAGCAACGAGGCCCCCAGCAATGAAGAGCTGGCCCGCAATCTCAGCG
ACCAAGCCAACACCAGCACCGCCGATCCCAGCGGCAGCCCGATTCTTGGCGTTGAAGGGCTGGAATACCGGTTGGGGGGC
TGTTGCAGTCCCTTGCCAGGCGAGCCGATCGTTGGCGCCGTTGCCCTTGGTAATCACGGCATCACCATTCACCGGCAGGA
TTGCGCCAACGTGGCGCAAACACCGGTGGAACGGCGCTTGCCCGTGCGCTGGAACCCCGTGGTCAGAGAACACCGCCGGC
GTTATCCGGTGCTGCTTCGCATCGAGGTGCTCGATCGCGTCGGCATCCTCAAAGACATCCTCACCCGGCTCTCCGATAGC
AGCATCAACGTCAGTGATGCGCGGGTTCGCACCGCCTATGGCAAACCAGCCCGCATCGATCTAAGGATCGAATTGAGCAG
CGCTGATCAACTGCAAGCCACCATGAACCAAATCCGCTCGATGGCGGATGTGCTGGACCTGGCCCGCTGCGGTAGCGCCT
GA

Upstream 100 bases:

>100_bases
TCCTCTAGCTGCAGCACAGGGGCTAACTCCATGGCGAGCTGCTGACCACGGTTGGGATTCTGCTCGCCGCTGAATACCAT
GACCTCAGTCTGACGGGGCC

Downstream 100 bases:

>100_bases
GATGGCCTGGCCATGGCTGCTGCTGCTCGGGGCAGCGCTGGGACTGATCTCCCTGCCCTATCAAGCCCTCTCCAGCCTTG
GGCTTGCCCTGCAGCAACAC

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 773; Mature: 773

Protein sequence:

>773_residues
MLDAAERFATSTASTLPGRAECDAPQLRQEIAGPDDYAIDLPAWLRQCIEHVPPGQGETCPTDPEGLLASAFDFAYQLHR
QQVRATGEPYIIHPVAVADLLRDIGASAAVIAAGFLHDVVEDTDVSLEEIEGHFGAEVRQLVEGVTKLGGIHFTNRTEAQ
AENLRKMFLAMASDIRVVLVKLADRLHNMRTLGALRPEKQQRIATETRDIYAPLANRLGIGRFKWELEDLAFKVLEPDDF
RSISQQVASKRSDREERLGVTVQLLRDRLENSGVHECEVVGRPKHLYGIWSKMQRQEKEFHEIFDVAAVRIVAPSVETCY
RALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGRHRPIEVQIRTGEMHEVAEYGIAAHWKYKEGGSPADGDEMAR
FNWLRQLVDWQKEEGGQDHNDYLRSIKEDLFDDEVFVFTPNGDVIGLRKGSTAVDFAYRIHSEVGNHCQGVRINDRLCPL
ATLLQNGDFVQIVTAKTAHPSLDWLNFVATPTARERIRQWYKRSHRDDNITRGREMLERELGRDGFDALLSSDPILRVAR
RCNLTGVDDLLASLGFGGVTVHQVTNRLREEMRLEEESSNEAPSNEELARNLSDQANTSTADPSGSPILGVEGLEYRLGG
CCSPLPGEPIVGAVALGNHGITIHRQDCANVAQTPVERRLPVRWNPVVREHRRRYPVLLRIEVLDRVGILKDILTRLSDS
SINVSDARVRTAYGKPARIDLRIELSSADQLQATMNQIRSMADVLDLARCGSA

Sequences:

>Translated_773_residues
MLDAAERFATSTASTLPGRAECDAPQLRQEIAGPDDYAIDLPAWLRQCIEHVPPGQGETCPTDPEGLLASAFDFAYQLHR
QQVRATGEPYIIHPVAVADLLRDIGASAAVIAAGFLHDVVEDTDVSLEEIEGHFGAEVRQLVEGVTKLGGIHFTNRTEAQ
AENLRKMFLAMASDIRVVLVKLADRLHNMRTLGALRPEKQQRIATETRDIYAPLANRLGIGRFKWELEDLAFKVLEPDDF
RSISQQVASKRSDREERLGVTVQLLRDRLENSGVHECEVVGRPKHLYGIWSKMQRQEKEFHEIFDVAAVRIVAPSVETCY
RALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGRHRPIEVQIRTGEMHEVAEYGIAAHWKYKEGGSPADGDEMAR
FNWLRQLVDWQKEEGGQDHNDYLRSIKEDLFDDEVFVFTPNGDVIGLRKGSTAVDFAYRIHSEVGNHCQGVRINDRLCPL
ATLLQNGDFVQIVTAKTAHPSLDWLNFVATPTARERIRQWYKRSHRDDNITRGREMLERELGRDGFDALLSSDPILRVAR
RCNLTGVDDLLASLGFGGVTVHQVTNRLREEMRLEEESSNEAPSNEELARNLSDQANTSTADPSGSPILGVEGLEYRLGG
CCSPLPGEPIVGAVALGNHGITIHRQDCANVAQTPVERRLPVRWNPVVREHRRRYPVLLRIEVLDRVGILKDILTRLSDS
SINVSDARVRTAYGKPARIDLRIELSSADQLQATMNQIRSMADVLDLARCGSA
>Mature_773_residues
MLDAAERFATSTASTLPGRAECDAPQLRQEIAGPDDYAIDLPAWLRQCIEHVPPGQGETCPTDPEGLLASAFDFAYQLHR
QQVRATGEPYIIHPVAVADLLRDIGASAAVIAAGFLHDVVEDTDVSLEEIEGHFGAEVRQLVEGVTKLGGIHFTNRTEAQ
AENLRKMFLAMASDIRVVLVKLADRLHNMRTLGALRPEKQQRIATETRDIYAPLANRLGIGRFKWELEDLAFKVLEPDDF
RSISQQVASKRSDREERLGVTVQLLRDRLENSGVHECEVVGRPKHLYGIWSKMQRQEKEFHEIFDVAAVRIVAPSVETCY
RALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGRHRPIEVQIRTGEMHEVAEYGIAAHWKYKEGGSPADGDEMAR
FNWLRQLVDWQKEEGGQDHNDYLRSIKEDLFDDEVFVFTPNGDVIGLRKGSTAVDFAYRIHSEVGNHCQGVRINDRLCPL
ATLLQNGDFVQIVTAKTAHPSLDWLNFVATPTARERIRQWYKRSHRDDNITRGREMLERELGRDGFDALLSSDPILRVAR
RCNLTGVDDLLASLGFGGVTVHQVTNRLREEMRLEEESSNEAPSNEELARNLSDQANTSTADPSGSPILGVEGLEYRLGG
CCSPLPGEPIVGAVALGNHGITIHRQDCANVAQTPVERRLPVRWNPVVREHRRRYPVLLRIEVLDRVGILKDILTRLSDS
SINVSDARVRTAYGKPARIDLRIELSSADQLQATMNQIRSMADVLDLARCGSA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=135, Percent_Identity=45.1851851851852, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1790082, Length=712, Percent_Identity=38.9044943820225, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1789147, Length=737, Percent_Identity=36.6350067842605, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI25143118, Length=129, Percent_Identity=38.7596899224806, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24650996, Length=140, Percent_Identity=36.4285714285714, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 86515; Mature: 86515

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAAERFATSTASTLPGRAECDAPQLRQEIAGPDDYAIDLPAWLRQCIEHVPPGQGETC
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEECHHHHHHHHHHCCCCCCCCC
PTDPEGLLASAFDFAYQLHRQQVRATGEPYIIHPVAVADLLRDIGASAAVIAAGFLHDVV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EDTDVSLEEIEGHFGAEVRQLVEGVTKLGGIHFTNRTEAQAENLRKMFLAMASDIRVVLV
HCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
KLADRLHNMRTLGALRPEKQQRIATETRDIYAPLANRLGIGRFKWELEDLAFKVLEPDDF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHCCCHHH
RSISQQVASKRSDREERLGVTVQLLRDRLENSGVHECEVVGRPKHLYGIWSKMQRQEKEF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
HEIFDVAAVRIVAPSVETCYRALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGRH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCC
RPIEVQIRTGEMHEVAEYGIAAHWKYKEGGSPADGDEMARFNWLRQLVDWQKEEGGQDHN
CCEEEEEECCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
DYLRSIKEDLFDDEVFVFTPNGDVIGLRKGSTAVDFAYRIHSEVGNHCQGVRINDRLCPL
HHHHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEECCCHHHH
ATLLQNGDFVQIVTAKTAHPSLDWLNFVATPTARERIRQWYKRSHRDDNITRGREMLERE
HHHHCCCCEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LGRDGFDALLSSDPILRVARRCNLTGVDDLLASLGFGGVTVHQVTNRLREEMRLEEESSN
HCCCHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
EAPSNEELARNLSDQANTSTADPSGSPILGVEGLEYRLGGCCSPLPGEPIVGAVALGNHG
CCCCHHHHHHHHHHHCCCCCCCCCCCCEECCCCCHHHCCCCCCCCCCCCCEEEEEECCCC
ITIHRQDCANVAQTPVERRLPVRWNPVVREHRRRYPVLLRIEVLDRVGILKDILTRLSDS
EEEECHHHHHHHHCCHHHCCCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCC
SINVSDARVRTAYGKPARIDLRIELSSADQLQATMNQIRSMADVLDLARCGSA
CCCCCHHHEEECCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDAAERFATSTASTLPGRAECDAPQLRQEIAGPDDYAIDLPAWLRQCIEHVPPGQGETC
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEECHHHHHHHHHHCCCCCCCCC
PTDPEGLLASAFDFAYQLHRQQVRATGEPYIIHPVAVADLLRDIGASAAVIAAGFLHDVV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EDTDVSLEEIEGHFGAEVRQLVEGVTKLGGIHFTNRTEAQAENLRKMFLAMASDIRVVLV
HCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
KLADRLHNMRTLGALRPEKQQRIATETRDIYAPLANRLGIGRFKWELEDLAFKVLEPDDF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHCCCHHH
RSISQQVASKRSDREERLGVTVQLLRDRLENSGVHECEVVGRPKHLYGIWSKMQRQEKEF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
HEIFDVAAVRIVAPSVETCYRALAVVHDTFRPIPGRFKDYIGLPKPNGYQSLHTAVIGRH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCC
RPIEVQIRTGEMHEVAEYGIAAHWKYKEGGSPADGDEMARFNWLRQLVDWQKEEGGQDHN
CCEEEEEECCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
DYLRSIKEDLFDDEVFVFTPNGDVIGLRKGSTAVDFAYRIHSEVGNHCQGVRINDRLCPL
HHHHHHHHHHCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEECCCHHHH
ATLLQNGDFVQIVTAKTAHPSLDWLNFVATPTARERIRQWYKRSHRDDNITRGREMLERE
HHHHCCCCEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LGRDGFDALLSSDPILRVARRCNLTGVDDLLASLGFGGVTVHQVTNRLREEMRLEEESSN
HCCCHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
EAPSNEELARNLSDQANTSTADPSGSPILGVEGLEYRLGGCCSPLPGEPIVGAVALGNHG
CCCCHHHHHHHHHHHCCCCCCCCCCCCEECCCCCHHHCCCCCCCCCCCCCEEEEEECCCC
ITIHRQDCANVAQTPVERRLPVRWNPVVREHRRRYPVLLRIEVLDRVGILKDILTRLSDS
EEEECHHHHHHHHCCHHHCCCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCC
SINVSDARVRTAYGKPARIDLRIELSSADQLQATMNQIRSMADVLDLARCGSA
CCCCCHHHEEECCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]