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The map label for this gene is tuf [H]

Identifier: 148243269

GI number: 148243269

Start: 1879850

End: 1881049

Strand: Direct

Name: tuf [H]

Synonym: SynRCC307_2170

Alternate gene names: 148243269

Gene position: 1879850-1881049 (Clockwise)

Preceding gene: 148243268

Following gene: 148243270

Centisome position: 84.49

GC content: 61.0

Gene sequence:

>1200_bases
ATGGCCCGCGAGAAGTTTGAGAGGAACAAACCTCACGTCAACATCGGCACCATCGGCCACGTTGACCACGGCAAAACAAC
CCTGACCGCCGCGATCACCAACGTGCTCGCGAAGAAGGGTCAGGCACAAGTTCAGAACTATGCCGATATCGACGGCGCTC
CCGAAGAGCGTGAGCGCGGCATCACCATCAACACCGCTCACGTTGAGTACGAGACCGACACTCGTCACTACGCCCACGTG
GACTGCCCCGGCCACGCGGACTACGTGAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCCATTCTGGTTTGCGC
CGCCACTGACGGCCCCATGGCCCAAACCAAGGAGCACATCCTTCTGGCCAAGCAGGTGGGCGTGCCCGCCCTGGTGGTGG
CCCTCAACAAGTGCGACATGGTCGACGACGAAGAGCTCATCGAGCTGGTGGAAATGGAAATCCGCGAGCTGCTCTCCAGC
TACGACTTCCCCGGTGATGACATCCCTGTCGTCCAGGTCTCTGGCCTCAAGGCCATCGAAGGCGAAGCCGAGTGGGAAGC
CAAGATCGATGAGCTGATGGCGGCGGTTGACGCCAACATCCCCGAGCCCGTGCGCGAGGTTGACAAGCCCTTCCTGATGG
CTGTGGAGGACGTGTTCTCCATCACCGGTCGCGGCACCGTGGCCACCGGCCGTATCGAGCGCGGCATCGTCAAAGTCGGC
GAAGAAATCGAAATCGTCGGCATCAAAGACACCCGTAAGTCCACCGTCACCGGCGTGGAAATGTTCCGCAAGCTGCTCGA
CGAGGGCATGGCTGGCGACAACGTTGGTCTGCTGCTGCGCGGCATCCAGAAGGAAGACATCGAGCGCGGCATGGTGCTGG
TGAAGCCCGGCTCCATCACTCCTCACACCAAGTTCGAGGGTGAGGTGTATGTGCTCAAGAAGGAAGAAGGCGGCCGCCAC
ACCCCCTTCTTCGCCGGTTATCGCCCGCAGTTCTACATCCGCACAACTGATGTGACCGGCCAAATCACTGCTTTCACCGA
CCGCGACGGCGGCAACGTGGAAATGGTGATGCCCGGTGACAACATCAAGATGACCGGTGAGCTGATCTGCCCTGTCGCCA
TCGAGCAAGGCATGCGCTTCGCTATCCGCGAAGGCGGCCGCACCATCGGTGCCGGCGTTGTGACCAAGATCATCGAGTGA

Upstream 100 bases:

>100_bases
GGAGGTTCCTCGCAACGTGGCCGAAGCCATTATTTCCAAGAATCAGGGAAATTCCTGATCCTTCCCCCCTCTTCATTTAC
CCCCCGATTCTTCATTAACA

Downstream 100 bases:

>100_bases
TCAAACTGGGTTGGGTCCTCAGCCTCTCCAGGCTGGGGGCTCAACCTCTGTTTCATTGCCGCCACTAGAGCGGCTGCACC
TCGACAACCCATCCCCCTCA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQVQNYADIDGAPEERERGITINTAHVEYETDTRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEELIELVEMEIRELLSS
YDFPGDDIPVVQVSGLKAIEGEAEWEAKIDELMAAVDANIPEPVREVDKPFLMAVEDVFSITGRGTVATGRIERGIVKVG
EEIEIVGIKDTRKSTVTGVEMFRKLLDEGMAGDNVGLLLRGIQKEDIERGMVLVKPGSITPHTKFEGEVYVLKKEEGGRH
TPFFAGYRPQFYIRTTDVTGQITAFTDRDGGNVEMVMPGDNIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVTKIIE

Sequences:

>Translated_399_residues
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQVQNYADIDGAPEERERGITINTAHVEYETDTRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEELIELVEMEIRELLSS
YDFPGDDIPVVQVSGLKAIEGEAEWEAKIDELMAAVDANIPEPVREVDKPFLMAVEDVFSITGRGTVATGRIERGIVKVG
EEIEIVGIKDTRKSTVTGVEMFRKLLDEGMAGDNVGLLLRGIQKEDIERGMVLVKPGSITPHTKFEGEVYVLKKEEGGRH
TPFFAGYRPQFYIRTTDVTGQITAFTDRDGGNVEMVMPGDNIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVTKIIE
>Mature_398_residues
AREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQVQNYADIDGAPEERERGITINTAHVEYETDTRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHILLAKQVGVPALVVALNKCDMVDDEELIELVEMEIRELLSSY
DFPGDDIPVVQVSGLKAIEGEAEWEAKIDELMAAVDANIPEPVREVDKPFLMAVEDVFSITGRGTVATGRIERGIVKVGE
EIEIVGIKDTRKSTVTGVEMFRKLLDEGMAGDNVGLLLRGIQKEDIERGMVLVKPGSITPHTKFEGEVYVLKKEEGGRHT
PFFAGYRPQFYIRTTDVTGQITAFTDRDGGNVEMVMPGDNIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVTKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=399, Percent_Identity=54.6365914786967, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=30.8390022675737, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=30.8390022675737, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=29.7539149888143, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=28.8590604026846, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI54607086, Length=370, Percent_Identity=28.9189189189189, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI194018522, Length=435, Percent_Identity=27.5862068965517, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194018520, Length=435, Percent_Identity=27.5862068965517, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194097354, Length=435, Percent_Identity=27.5862068965517, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI46094014, Length=435, Percent_Identity=29.1954022988506, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=28.0898876404494, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI157426893, Length=274, Percent_Identity=25.5474452554745, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI53729339, Length=237, Percent_Identity=32.9113924050633, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI53729337, Length=237, Percent_Identity=32.9113924050633, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1790412, Length=398, Percent_Identity=74.3718592964824, Blast_Score=586, Evalue=1e-169,
Organism=Escherichia coli, GI1789737, Length=398, Percent_Identity=74.3718592964824, Blast_Score=586, Evalue=1e-168,
Organism=Escherichia coli, GI2367247, Length=335, Percent_Identity=31.6417910447761, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789108, Length=331, Percent_Identity=29.3051359516616, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=31.1203319502075, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788922, Length=330, Percent_Identity=24.2424242424242, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=401, Percent_Identity=51.3715710723192, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=44.6153846153846, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=449, Percent_Identity=30.5122494432071, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=449, Percent_Identity=30.5122494432071, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=29.1571753986333, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=29.1954022988506, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=27.4336283185841, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=28.8018433179723, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=30.1470588235294, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17509919, Length=269, Percent_Identity=27.8810408921933, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=40.1360544217687, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=28.2051282051282, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=177, Percent_Identity=33.8983050847458, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=62.72040302267, Blast_Score=499, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=30.3370786516854, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=30.3370786516854, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=456, Percent_Identity=25.219298245614, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320377, Length=446, Percent_Identity=26.6816143497758, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=280, Percent_Identity=27.5, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=29.2887029288703, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=57.9746835443038, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=57.9746835443038, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI19921738, Length=391, Percent_Identity=46.0358056265985, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI45553807, Length=452, Percent_Identity=30.3097345132743, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45553816, Length=452, Percent_Identity=30.3097345132743, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24651721, Length=452, Percent_Identity=30.3097345132743, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17864154, Length=452, Percent_Identity=30.3097345132743, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=30.9576837416481, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=30.9576837416481, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=29.7539149888143, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.3630363036304, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17137380, Length=451, Percent_Identity=27.4944567627494, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24647048, Length=266, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24647046, Length=266, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28572034, Length=175, Percent_Identity=36, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78706572, Length=258, Percent_Identity=28.2945736434109, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43584; Mature: 43453

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQVQNYADIDGAPEERERG
CCCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHCC
ITINTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHE
LLAKQVGVPALVVALNKCDMVDDEELIELVEMEIRELLSSYDFPGDDIPVVQVSGLKAIE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEC
GEAEWEAKIDELMAAVDANIPEPVREVDKPFLMAVEDVFSITGRGTVATGRIERGIVKVG
CCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHCC
EEIEIVGIKDTRKSTVTGVEMFRKLLDEGMAGDNVGLLLRGIQKEDIERGMVLVKPGSIT
CEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHEEECCCHHHHHCCEEEEECCCCC
PHTKFEGEVYVLKKEEGGRHTPFFAGYRPQFYIRTTDVTGQITAFTDRDGGNVEMVMPGD
CCCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCEEEEEEECCCCCCEEEEECCC
NIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVTKIIE
CEEEECEEECHHHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AREKFERNKPHVNIGTIGHVDHGKTTLTAAITNVLAKKGQAQVQNYADIDGAPEERERG
CCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHCC
ITINTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGAILVCAATDGPMAQTKEHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHE
LLAKQVGVPALVVALNKCDMVDDEELIELVEMEIRELLSSYDFPGDDIPVVQVSGLKAIE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEC
GEAEWEAKIDELMAAVDANIPEPVREVDKPFLMAVEDVFSITGRGTVATGRIERGIVKVG
CCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHCC
EEIEIVGIKDTRKSTVTGVEMFRKLLDEGMAGDNVGLLLRGIQKEDIERGMVLVKPGSIT
CEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHEEECCCHHHHHCCEEEEECCCCC
PHTKFEGEVYVLKKEEGGRHTPFFAGYRPQFYIRTTDVTGQITAFTDRDGGNVEMVMPGD
CCCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCEEEEEEECCCCCCEEEEECCC
NIKMTGELICPVAIEQGMRFAIREGGRTIGAGVVTKIIE
CEEEECEEECHHHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA