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The map label for this gene is purB [H]

Identifier: 148242998

GI number: 148242998

Start: 1652329

End: 1653624

Strand: Direct

Name: purB [H]

Synonym: SynRCC307_1899

Alternate gene names: 148242998

Gene position: 1652329-1653624 (Clockwise)

Preceding gene: 148242997

Following gene: 148243004

Centisome position: 74.26

GC content: 63.43

Gene sequence:

>1296_bases
TTGATCGAGCGCTACACCCTGCCGGAGATGGGCGGGATCTGGACGGAAGACGCCAAGTTCCAAAGCTGGCTGGACGTTGA
AATCGCTGTCTGCGAAGCCCAGTCCGAGCTGGGCCGCGTGCCTGCTGAAGCACTCGCCACGATCAAGAACAAAGCAGCCT
TCAGCCGCGAGCGGATCCTCGAGATCGAGGCCGAGGTGCGCCACGACGTGATCGCCTTCCTCACCAACGTGAACGAACAC
GTGGGTGATGCCGGGCGCTACATCCATGTGGGCATGACCAGCTCCGATGTGCTCGACACCGGCCTGGCGCTGCAACTCAA
GGCCTCGGTGAAGCTGCTGCGCAGCGAGCTCGACAAGCTCGCAGAAGCCATCCGCGTGCAAGCACGGGCCCACAAAAACA
CCGTGATGATCGGGCGCTCCCATGCCATCCACGGCGAGCCGATCACCTTTGGCTTCAAGCTGGCCGGCTGGCTCGCGGAG
ACCGAGCGCAACATCAAACGCCTCAAGCGTCTGCAGAAGGCCGTCAGCGTGGGCCAGATCAGTGGCGCCATGGGCAACTA
CGCCAACACCGAACCGCAGATCGAAGAACGGGCCTGTGAGCTGCTGGGGCTTCGGCCCGATCCAGCCAGCACCCAGGTGA
TCAGCCGCGACCGCCACGCCGATTACGTGCAGACCCTGGCCCTGGTGGGCACGGCCCTAGAGCGCTTCTCCACAGAGATT
CGCAACCTGCAGCGCAGCGATGTGCTCGAGGTGGAAGAGGCCTTTGCCAAGGGGCAAAAGGGCAGCTCAGCCATGCCCCA
CAAACGCAACCCGATCCGCAGCGAACGCATCAGCGGCTTGGCGCGGGTGCTGCGCAGCTACACCGTGGCAGCTCTGGAAA
ACGTGGCGCTGTGGCACGAGCGCGACATCAGCCACAGCTCGGTGGAGCGCATGATGCTTCCTGATGTCAGCGTCACCCTG
CATTTCATGCTGCGGGAGATGACGAAGGTCGTGAGCGGTCTGCAGGTCTACCCCGACACCATGGAGCGCAACATGAACCG
CTACGGCGGTGTGGTGTTCAGCCAAACGGTGTTGCTGGCCTTAGTGGACAGCGGCATGAGCCGCGAAGACGCCTACCGGC
TGGTGCAGGGCCATGCCCATTCCGCCTGGAATGTCGAAGGCGGCAACTTCCGCAAGGCGCTCGAGAACGATCCCGCAGTC
ACCGATCGCATCAGCGCTGAGCGCCTGGCCGATTGCTTCAGCACCGAGCGGCATCAAGCCCAGCTCGACACCATCTACGC
CCGCTTGGCAATCTGA

Upstream 100 bases:

>100_bases
GCTGGGTGCGTCAGCAGGCCTGGGGCCCAGCGATCCAATGGGAAGTGGCCCGCCGCAGTTTGAGCCCCGGGTAATTTGGT
CGATTGGCCGAGCAAAACCC

Downstream 100 bases:

>100_bases
GCGATCGGCGTTGGCGCGCCAGTTCGCCCGGTTGAGGGAGTCATCCACGATCTGCGCCGGACTTTGGCGCGCGGGGTGGT
CAACAACCCGAGGCTCTTCG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPEMGGIWTEDAKFQSWLDVEIAVCEAQSELGRVPAEALATIKNKAAFSRERILEIEAEVRHDVIAFLTNVNEH
VGDAGRYIHVGMTSSDVLDTGLALQLKASVKLLRSELDKLAEAIRVQARAHKNTVMIGRSHAIHGEPITFGFKLAGWLAE
TERNIKRLKRLQKAVSVGQISGAMGNYANTEPQIEERACELLGLRPDPASTQVISRDRHADYVQTLALVGTALERFSTEI
RNLQRSDVLEVEEAFAKGQKGSSAMPHKRNPIRSERISGLARVLRSYTVAALENVALWHERDISHSSVERMMLPDVSVTL
HFMLREMTKVVSGLQVYPDTMERNMNRYGGVVFSQTVLLALVDSGMSREDAYRLVQGHAHSAWNVEGGNFRKALENDPAV
TDRISAERLADCFSTERHQAQLDTIYARLAI

Sequences:

>Translated_431_residues
MIERYTLPEMGGIWTEDAKFQSWLDVEIAVCEAQSELGRVPAEALATIKNKAAFSRERILEIEAEVRHDVIAFLTNVNEH
VGDAGRYIHVGMTSSDVLDTGLALQLKASVKLLRSELDKLAEAIRVQARAHKNTVMIGRSHAIHGEPITFGFKLAGWLAE
TERNIKRLKRLQKAVSVGQISGAMGNYANTEPQIEERACELLGLRPDPASTQVISRDRHADYVQTLALVGTALERFSTEI
RNLQRSDVLEVEEAFAKGQKGSSAMPHKRNPIRSERISGLARVLRSYTVAALENVALWHERDISHSSVERMMLPDVSVTL
HFMLREMTKVVSGLQVYPDTMERNMNRYGGVVFSQTVLLALVDSGMSREDAYRLVQGHAHSAWNVEGGNFRKALENDPAV
TDRISAERLADCFSTERHQAQLDTIYARLAI
>Mature_431_residues
MIERYTLPEMGGIWTEDAKFQSWLDVEIAVCEAQSELGRVPAEALATIKNKAAFSRERILEIEAEVRHDVIAFLTNVNEH
VGDAGRYIHVGMTSSDVLDTGLALQLKASVKLLRSELDKLAEAIRVQARAHKNTVMIGRSHAIHGEPITFGFKLAGWLAE
TERNIKRLKRLQKAVSVGQISGAMGNYANTEPQIEERACELLGLRPDPASTQVISRDRHADYVQTLALVGTALERFSTEI
RNLQRSDVLEVEEAFAKGQKGSSAMPHKRNPIRSERISGLARVLRSYTVAALENVALWHERDISHSSVERMMLPDVSVTL
HFMLREMTKVVSGLQVYPDTMERNMNRYGGVVFSQTVLLALVDSGMSREDAYRLVQGHAHSAWNVEGGNFRKALENDPAV
TDRISAERLADCFSTERHQAQLDTIYARLAI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=403, Percent_Identity=27.5434243176179, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI183227688, Length=380, Percent_Identity=27.3684210526316, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1787376, Length=358, Percent_Identity=27.6536312849162, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790398, Length=323, Percent_Identity=26.3157894736842, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=391, Percent_Identity=26.8542199488491, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564234, Length=351, Percent_Identity=28.7749287749288, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323391, Length=414, Percent_Identity=27.7777777777778, Blast_Score=153, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24647570, Length=386, Percent_Identity=26.9430051813472, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48126; Mature: 48126

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMGGIWTEDAKFQSWLDVEIAVCEAQSELGRVPAEALATIKNKAAFSRERIL
CCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EIEAEVRHDVIAFLTNVNEHVGDAGRYIHVGMTSSDVLDTGLALQLKASVKLLRSELDKL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCEEHHHHHHHHHHHHHHH
AEAIRVQARAHKNTVMIGRSHAIHGEPITFGFKLAGWLAETERNIKRLKRLQKAVSVGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGNYANTEPQIEERACELLGLRPDPASTQVISRDRHADYVQTLALVGTALERFSTEI
HHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RNLQRSDVLEVEEAFAKGQKGSSAMPHKRNPIRSERISGLARVLRSYTVAALENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERMMLPDVSVTLHFMLREMTKVVSGLQVYPDTMERNMNRYGGVVFSQTVLLA
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
LVDSGMSREDAYRLVQGHAHSAWNVEGGNFRKALENDPAVTDRISAERLADCFSTERHQA
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
QLDTIYARLAI
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPEMGGIWTEDAKFQSWLDVEIAVCEAQSELGRVPAEALATIKNKAAFSRERIL
CCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EIEAEVRHDVIAFLTNVNEHVGDAGRYIHVGMTSSDVLDTGLALQLKASVKLLRSELDKL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCEEHHHHHHHHHHHHHHH
AEAIRVQARAHKNTVMIGRSHAIHGEPITFGFKLAGWLAETERNIKRLKRLQKAVSVGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGNYANTEPQIEERACELLGLRPDPASTQVISRDRHADYVQTLALVGTALERFSTEI
HHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RNLQRSDVLEVEEAFAKGQKGSSAMPHKRNPIRSERISGLARVLRSYTVAALENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERMMLPDVSVTLHFMLREMTKVVSGLQVYPDTMERNMNRYGGVVFSQTVLLA
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
LVDSGMSREDAYRLVQGHAHSAWNVEGGNFRKALENDPAVTDRISAERLADCFSTERHQA
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
QLDTIYARLAI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]