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The map label for this gene is ispF [H]

Identifier: 148242829

GI number: 148242829

Start: 1515375

End: 1515860

Strand: Direct

Name: ispF [H]

Synonym: SynRCC307_1730

Alternate gene names: 148242829

Gene position: 1515375-1515860 (Clockwise)

Preceding gene: 148242828

Following gene: 148242830

Centisome position: 68.11

GC content: 63.99

Gene sequence:

>486_bases
ATGAACATCCGCGTTGGCAACGGCTACGACATCCACCGGCTGGTGCCGGGCCGCCCACTGATCCTGGGCGGCCAAACCCT
CGAGCACCCAGGTGGCCTGGGCCTCGATGGCCACAGCGATGCTGATGTGCTGGTGCACGCGATCATGGACGCCTTGCTCG
GCGCCCTCTCGCTAGGCGATATCGGCAAATACTTCCCGCCCACCGATCCCAAATGGAAAGGGGCCGACAGCCTGCTTCTG
CTGGAGCAAGTGATGGGCCTGGTGCGTGAGCGCGGCTGGAGCGTGGTGAATGTGGATTCGGTGGTGATCGCCGAGCGGCC
CAAGCTCAAGCCCCACATCGAGGCGATGCGCGCAGCGATTGCAGAGCGCATGGGCCTGGATCCTGAGCAGGTGGGCGTGA
AGGCCACCACCAACGAAAAGCTGGGAGCTGAAGGCAGGGAAGAAGGCATTTCCTGCCAGGCCGTGGCCCTGCTCTTCAAG
CCATGA

Upstream 100 bases:

>100_bases
ACAATCTCGAACCGGGGCAGCTGCTGGCCCCAGGCGCTGTGGTGGTGGTGATGGGGCGCGAGGGCACCAGGCTGCAGGTG
CTGGCTTCTAAGGTCGAGCC

Downstream 100 bases:

>100_bases
GCTGGCTGCCCGCGCTGCTGCTGAGCCTGCTGCTTTGGGCCGCCCCAGCCTGGGCGGTGCGCCCCCTCGATCCAGCCGCT
GGTGTGGTGGAGGTGCTGCA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MNIRVGNGYDIHRLVPGRPLILGGQTLEHPGGLGLDGHSDADVLVHAIMDALLGALSLGDIGKYFPPTDPKWKGADSLLL
LEQVMGLVRERGWSVVNVDSVVIAERPKLKPHIEAMRAAIAERMGLDPEQVGVKATTNEKLGAEGREEGISCQAVALLFK
P

Sequences:

>Translated_161_residues
MNIRVGNGYDIHRLVPGRPLILGGQTLEHPGGLGLDGHSDADVLVHAIMDALLGALSLGDIGKYFPPTDPKWKGADSLLL
LEQVMGLVRERGWSVVNVDSVVIAERPKLKPHIEAMRAAIAERMGLDPEQVGVKATTNEKLGAEGREEGISCQAVALLFK
P
>Mature_161_residues
MNIRVGNGYDIHRLVPGRPLILGGQTLEHPGGLGLDGHSDADVLVHAIMDALLGALSLGDIGKYFPPTDPKWKGADSLLL
LEQVMGLVRERGWSVVNVDSVVIAERPKLKPHIEAMRAAIAERMGLDPEQVGVKATTNEKLGAEGREEGISCQAVALLFK
P

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=158, Percent_Identity=50.6329113924051, Blast_Score=146, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17244; Mature: 17244

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRVGNGYDIHRLVPGRPLILGGQTLEHPGGLGLDGHSDADVLVHAIMDALLGALSLGD
CEECCCCCCCEEEECCCCEEEECCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
IGKYFPPTDPKWKGADSLLLLEQVMGLVRERGWSVVNVDSVVIAERPKLKPHIEAMRAAI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCHHHHHHHHHH
AERMGLDPEQVGVKATTNEKLGAEGREEGISCQAVALLFKP
HHHHCCCHHHCCCCCCCCCCCCCCCHHCCCCEEEEEEEECC
>Mature Secondary Structure
MNIRVGNGYDIHRLVPGRPLILGGQTLEHPGGLGLDGHSDADVLVHAIMDALLGALSLGD
CEECCCCCCCEEEECCCCEEEECCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
IGKYFPPTDPKWKGADSLLLLEQVMGLVRERGWSVVNVDSVVIAERPKLKPHIEAMRAAI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCHHHHHHHHHH
AERMGLDPEQVGVKATTNEKLGAEGREEGISCQAVALLFKP
HHHHCCCHHHCCCCCCCCCCCCCCCHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA