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The map label for this gene is pykF [H]

Identifier: 148242498

GI number: 148242498

Start: 1243875

End: 1245659

Strand: Direct

Name: pykF [H]

Synonym: SynRCC307_1399

Alternate gene names: 148242498

Gene position: 1243875-1245659 (Clockwise)

Preceding gene: 148242494

Following gene: 148242499

Centisome position: 55.91

GC content: 62.58

Gene sequence:

>1785_bases
ATGCCCCTGCCCGATCATCAGCGACGCACCAAAATCGTCGCCACAATCGGGCCGGCCACAGAATCACCAGACAGCATTCG
GCAGCTGATTCTGGCCGGTGCCACAACCTTCCGACTGAACTTCTCCCACGGAGATCACGAGGAGCATGCCCAGCGCGCCG
CCACGATCAGACAGGTGGCCCATGAACTGGGAGTTCACGTTGGAATCCTGCAAGACCTGCAAGGGCCCAAGATCCGCCTC
GGCCGTTTTGCCGACGGTCCAATCACCGTGGCCAAGGGGGATTCCTTCGCCCTAACGGCGCGCGATGTGCCCTGCACCCA
AAGCATCGCCACAGTCACCTACGACAAGCTGGCCAGCGAAGTGGAGCCCGGCAGCCGCATCCTTCTTGATGACGGCAAGG
TGGAGATGAAGGTCGACTGGATCGACCAGGCCGAACAGACCCTGCACTGCAAGATCACCGTTGGCGGCAAGCTCTCCAAC
AACAAAGGCGTCAACTTCCCCGACTGCCTGCTCTCGGTCCGCGCGCTGACGGCCAAAGACCGTAATGACCTGGCCTTTGG
TTTGCAGCTCGGCGTCGACTGGGTGGCGCTCAGCTTTGTGCGCAACCCCTCGGATATGCAGGAGATCAAGGAGCTAATCA
GCTCCTTGGGCCATGCCACACCCGTGGTAGCCAAGATCGAAAAATTTGAGGCGATCGATCAGATCGACGCCATCCTGCCG
CTCTGCGACGGCGTCATGGTGGCCCGTGGCGACCTGGGCGTGGAGATGGCGGCCGAGGAAGTGCCCCTGCTGCAGAAGGA
GCTGATCCGCAAGGCCAACTCCATGGGCATCCCGATCATCACGGCCACCCAGATGCTCGACAGCATGGTGGCCAATGCCC
GGCCCACCCGCGCTGAGGTGAGCGATGTGGCGAACGCGATCCTCGATGGCACCGATGCGGTGATGCTCTCCAACGAAACC
GCCGTTGGCGACTACGCCGTTGAGGCCGTGGAAACCATGGACCGGATCGCCAGGCGCATTGAAAAGGATTACCCAAACCG
CCAGCCCGACACCCAGCTGGCCTGCACGATCCCCAACGCCATCAGCATGGCGGTGAGCACGATCGCCACCCAACTCGATG
CGGCCGCGATCTTGACGCTCACCAAAAGTGGCGCCACGGGGCGCACCGTCAGCAAGTTCCGCCCCTCAACGCCAATCCTG
GCGATCACCAGCGACGTCAAGGTGGCCCGCCAGCTGCAACTGGTTTGGGGTGTGAATCCCCTGGTGATTGCCACCCAGGC
CTCCACCAGCCGCACCTTCAGCCTTGCCATGGGTGTTGCCCAAGAACAGAAGTTGCTGCAAGACGGCGATCTGGTGATCC
AAACCGCTGGAACTCTGGCCGGCGTCAGTGGATCCACCGATCTGGTCAAGGTGGGCATCGTTTCAGCCGTGCTTGGACGG
GGCACAGGTTTTGGCAGCCAACCGGTGAGCGGCCAGGTGTGCATTGCCAATTCACCCCAGGAAGCCGCCCGCATCGAAGC
CGGCGAGATCTTGGTGGTGCCGCACACCGATGCGAACTACCTCGAAGCCATTCGCCGCTGCGGGGGGGTGATCGCTGAAG
AAGATGGCAGCCAAAGCCATGCCGCTGTGATCGGCCAGCGTCTCGGGATCCCAGTCATCACAGGGGTGGCCAATGCCACC
CGCGACGTGCGCCAAGGAGAGGTGGTTACCCTCGATGTCCGCTACGGGATGGTGCACCGCGGCACCCACGTCCATGACCA
CAGCAGCGCCGACACGATCGGTTGA

Upstream 100 bases:

>100_bases
GATCGGGGTACAGGGCTGTGTTGCGCGCTTGCCGCTGATAATTGTTGAGATCCATTGGAACGTTCGGGTGGTAGCTTCCG
CAACGCTTCAGGCCCGGTCC

Downstream 100 bases:

>100_bases
ATCCGCCGCGGTGGTCGTTGCCCGACTACCGCTCCGTGAAACCGTCGGAATGGCCCTGGGCGCGCTTGGGGCCAACCGCT
TGCGCTCTCTGCTGACGATG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MPLPDHQRRTKIVATIGPATESPDSIRQLILAGATTFRLNFSHGDHEEHAQRAATIRQVAHELGVHVGILQDLQGPKIRL
GRFADGPITVAKGDSFALTARDVPCTQSIATVTYDKLASEVEPGSRILLDDGKVEMKVDWIDQAEQTLHCKITVGGKLSN
NKGVNFPDCLLSVRALTAKDRNDLAFGLQLGVDWVALSFVRNPSDMQEIKELISSLGHATPVVAKIEKFEAIDQIDAILP
LCDGVMVARGDLGVEMAAEEVPLLQKELIRKANSMGIPIITATQMLDSMVANARPTRAEVSDVANAILDGTDAVMLSNET
AVGDYAVEAVETMDRIARRIEKDYPNRQPDTQLACTIPNAISMAVSTIATQLDAAAILTLTKSGATGRTVSKFRPSTPIL
AITSDVKVARQLQLVWGVNPLVIATQASTSRTFSLAMGVAQEQKLLQDGDLVIQTAGTLAGVSGSTDLVKVGIVSAVLGR
GTGFGSQPVSGQVCIANSPQEAARIEAGEILVVPHTDANYLEAIRRCGGVIAEEDGSQSHAAVIGQRLGIPVITGVANAT
RDVRQGEVVTLDVRYGMVHRGTHVHDHSSADTIG

Sequences:

>Translated_594_residues
MPLPDHQRRTKIVATIGPATESPDSIRQLILAGATTFRLNFSHGDHEEHAQRAATIRQVAHELGVHVGILQDLQGPKIRL
GRFADGPITVAKGDSFALTARDVPCTQSIATVTYDKLASEVEPGSRILLDDGKVEMKVDWIDQAEQTLHCKITVGGKLSN
NKGVNFPDCLLSVRALTAKDRNDLAFGLQLGVDWVALSFVRNPSDMQEIKELISSLGHATPVVAKIEKFEAIDQIDAILP
LCDGVMVARGDLGVEMAAEEVPLLQKELIRKANSMGIPIITATQMLDSMVANARPTRAEVSDVANAILDGTDAVMLSNET
AVGDYAVEAVETMDRIARRIEKDYPNRQPDTQLACTIPNAISMAVSTIATQLDAAAILTLTKSGATGRTVSKFRPSTPIL
AITSDVKVARQLQLVWGVNPLVIATQASTSRTFSLAMGVAQEQKLLQDGDLVIQTAGTLAGVSGSTDLVKVGIVSAVLGR
GTGFGSQPVSGQVCIANSPQEAARIEAGEILVVPHTDANYLEAIRRCGGVIAEEDGSQSHAAVIGQRLGIPVITGVANAT
RDVRQGEVVTLDVRYGMVHRGTHVHDHSSADTIG
>Mature_593_residues
PLPDHQRRTKIVATIGPATESPDSIRQLILAGATTFRLNFSHGDHEEHAQRAATIRQVAHELGVHVGILQDLQGPKIRLG
RFADGPITVAKGDSFALTARDVPCTQSIATVTYDKLASEVEPGSRILLDDGKVEMKVDWIDQAEQTLHCKITVGGKLSNN
KGVNFPDCLLSVRALTAKDRNDLAFGLQLGVDWVALSFVRNPSDMQEIKELISSLGHATPVVAKIEKFEAIDQIDAILPL
CDGVMVARGDLGVEMAAEEVPLLQKELIRKANSMGIPIITATQMLDSMVANARPTRAEVSDVANAILDGTDAVMLSNETA
VGDYAVEAVETMDRIARRIEKDYPNRQPDTQLACTIPNAISMAVSTIATQLDAAAILTLTKSGATGRTVSKFRPSTPILA
ITSDVKVARQLQLVWGVNPLVIATQASTSRTFSLAMGVAQEQKLLQDGDLVIQTAGTLAGVSGSTDLVKVGIVSAVLGRG
TGFGSQPVSGQVCIANSPQEAARIEAGEILVVPHTDANYLEAIRRCGGVIAEEDGSQSHAAVIGQRLGIPVITGVANATR
DVRQGEVVTLDVRYGMVHRGTHVHDHSSADTIG

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=492, Percent_Identity=36.1788617886179, Blast_Score=302, Evalue=7e-82,
Organism=Homo sapiens, GI33286420, Length=492, Percent_Identity=36.1788617886179, Blast_Score=302, Evalue=7e-82,
Organism=Homo sapiens, GI33286418, Length=492, Percent_Identity=36.1788617886179, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=37.3983739837398, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=37.3983739837398, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI310128732, Length=293, Percent_Identity=35.4948805460751, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI310128730, Length=293, Percent_Identity=35.4948805460751, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI310128736, Length=241, Percent_Identity=31.5352697095436, Blast_Score=133, Evalue=6e-31,
Organism=Homo sapiens, GI310128734, Length=241, Percent_Identity=31.5352697095436, Blast_Score=133, Evalue=6e-31,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=30.5555555555556, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=40.8898305084746, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1788160, Length=474, Percent_Identity=40.9282700421941, Blast_Score=319, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17544584, Length=431, Percent_Identity=40.6032482598608, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=38.4458077709611, Blast_Score=287, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=38.4458077709611, Blast_Score=287, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=38.4458077709611, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=38.4458077709611, Blast_Score=286, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6319279, Length=488, Percent_Identity=38.5245901639344, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=37.1663244353183, Blast_Score=279, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=36.5111561866126, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=36.5111561866126, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24648966, Length=424, Percent_Identity=36.7924528301887, Blast_Score=256, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24581235, Length=488, Percent_Identity=31.7622950819672, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24646914, Length=314, Percent_Identity=36.3057324840764, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 63179; Mature: 63048

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPDHQRRTKIVATIGPATESPDSIRQLILAGATTFRLNFSHGDHEEHAQRAATIRQVA
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHH
HELGVHVGILQDLQGPKIRLGRFADGPITVAKGDSFALTARDVPCTQSIATVTYDKLASE
HHHCCEEEHHHHCCCCCEEECEECCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHH
VEPGSRILLDDGKVEMKVDWIDQAEQTLHCKITVGGKLSNNKGVNFPDCLLSVRALTAKD
CCCCCEEEEECCCEEEEEEECCCCCCEEEEEEEECCEECCCCCCCCHHHHHHHHHHHCCC
RNDLAFGLQLGVDWVALSFVRNPSDMQEIKELISSLGHATPVVAKIEKFEAIDQIDAILP
CCCEEEEEHHCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LCDGVMVARGDLGVEMAAEEVPLLQKELIRKANSMGIPIITATQMLDSMVANARPTRAEV
HHCCEEEECCCCCCHHHHHHCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGDYAVEAVETMDRIARRIEKDYPNRQPDTQLACTIPNA
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHH
ISMAVSTIATQLDAAAILTLTKSGATGRTVSKFRPSTPILAITSDVKVARQLQLVWGVNP
HHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHCCCCCCEEEEECCHHHHHHHHHHCCCCC
LVIATQASTSRTFSLAMGVAQEQKLLQDGDLVIQTAGTLAGVSGSTDLVKVGIVSAVLGR
EEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHC
GTGFGSQPVSGQVCIANSPQEAARIEAGEILVVPHTDANYLEAIRRCGGVIAEEDGSQSH
CCCCCCCCCCCEEEECCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHCCEEECCCCCCHH
AAVIGQRLGIPVITGVANATRDVRQGEVVTLDVRYGMVHRGTHVHDHSSADTIG
HHHHHHHCCCCCEECCHHHHHHHCCCCEEEEEEEECEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLPDHQRRTKIVATIGPATESPDSIRQLILAGATTFRLNFSHGDHEEHAQRAATIRQVA
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHH
HELGVHVGILQDLQGPKIRLGRFADGPITVAKGDSFALTARDVPCTQSIATVTYDKLASE
HHHCCEEEHHHHCCCCCEEECEECCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHH
VEPGSRILLDDGKVEMKVDWIDQAEQTLHCKITVGGKLSNNKGVNFPDCLLSVRALTAKD
CCCCCEEEEECCCEEEEEEECCCCCCEEEEEEEECCEECCCCCCCCHHHHHHHHHHHCCC
RNDLAFGLQLGVDWVALSFVRNPSDMQEIKELISSLGHATPVVAKIEKFEAIDQIDAILP
CCCEEEEEHHCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LCDGVMVARGDLGVEMAAEEVPLLQKELIRKANSMGIPIITATQMLDSMVANARPTRAEV
HHCCEEEECCCCCCHHHHHHCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGDYAVEAVETMDRIARRIEKDYPNRQPDTQLACTIPNA
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHH
ISMAVSTIATQLDAAAILTLTKSGATGRTVSKFRPSTPILAITSDVKVARQLQLVWGVNP
HHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHCCCCCCEEEEECCHHHHHHHHHHCCCCC
LVIATQASTSRTFSLAMGVAQEQKLLQDGDLVIQTAGTLAGVSGSTDLVKVGIVSAVLGR
EEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHC
GTGFGSQPVSGQVCIANSPQEAARIEAGEILVVPHTDANYLEAIRRCGGVIAEEDGSQSH
CCCCCCCCCCCEEEECCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHCCEEECCCCCCHH
AAVIGQRLGIPVITGVANATRDVRQGEVVTLDVRYGMVHRGTHVHDHSSADTIG
HHHHHHHCCCCCEECCHHHHHHHCCCCEEEEEEEECEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]