| Definition | Synechococcus sp. RCC307, complete genome. |
|---|---|
| Accession | NC_009482 |
| Length | 2,224,914 |
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The map label for this gene is murF [H]
Identifier: 148242245
GI number: 148242245
Start: 1011995
End: 1013389
Strand: Direct
Name: murF [H]
Synonym: SynRCC307_1146
Alternate gene names: 148242245
Gene position: 1011995-1013389 (Clockwise)
Preceding gene: 148242244
Following gene: 148242246
Centisome position: 45.48
GC content: 61.72
Gene sequence:
>1395_bases ATGAGCCCCTCGCTTTGCCTGCAGGATCTGGCCCGCTTGTTGGATGCTCCGGTGCAGCGGCGCAGCGCCCCTGCTGATCC AACGACCTTGGCATGGACGTTTGAACACATCAGCACCGATAGCCGCGCGCTGCAGCCGGGTGATTTGTTTATCCCGTTGC GGGGCGAACGTTTTGATGGCCACGATTTTCTTGATGCGGCCGTCAGTGTTGGCGTTGCCGGTGCCCTGATCGCCCGCGAT TGGGCTGGTTACGTGCCTGAGCAGCTGGCCACTGTGGTGGTCGATGACACCCTCAGCGCTTATCAGCAGATCGCCTTGGC CTGGCGCCGACAGCTCCATTGCCCCGTGGTGGCCGTGACCGGTTCAGCCGGTAAAACCACAACCCGTGAGCTAATCAAGG CTTGCTTGTCTCCATTAGGAGCCATTGAGAGCAGTGTTGGCAACGAAAACAACGACATCGGTGTTCCCCGCACGATGTTG CGCAGCAATAGCAGTCATGCCGGATTGGTGCTGGAGATGGGGATGCGCGGCCTGGGCGAAATTCATCGCCTCAGCGTTTG CGCGGAACCCGATGTGGCGGTGATCACCAACATCGGCACCGCCCACATCGGGCGCCTGGGGAGTCGGGCTGCCATTGCCC AGGCCAAATGTGAGATCACCGATCGCCTTAAGCCCTCGGGTTTGCTCGTGATTCCCGCTGGTGATCCACTGCTGGAAGCC GAGGTCAGCCGTGTCTGGAGCGGGCGTGTACAACGGGTGGCGCTCAGTGATGACCCCCTGGAGCCAGGCACACCAGCCCC CGATTGGGTCGCTCAACTCCAGGGGCAGAGCATCGAATTGGCCAGCCAAGGGTTGAAGCTCATCAACCCATTGGAGGGGC GCCACAACGCCCGTAATTTCCTGCTGGCTCTCGCTGCTGCTCAGGAGTTGGGCGTCACGGCAGAACAGCTGCGTGATCTC TCGGTGTCGCTGCCTGGTGGCCGCGCGCGGCGCTTGAGGATCAATGGGGTCAACATCCTCGATGAGACCTACAACGCTTC ACCAGAAGCGATGTTGGCCAGCCTGGAGCTGCTGAAGCAGCAGCCCGGCGGCCATCGTTATGCCGTGGTGGGCACGATGC TGGAGCTGGGCGATCAAAGTTTGTCCCTGCATCGCCAAGTGGGGGAAAAAGCGACGGCCCTGGGCCTCGATGGGCTGGTG GTGGTGGCCTCTGGGCCCGAAGCCAGCGCTCTGATGCAGGGTGCTGCTGGGCTCAGCAAGTTGGTCTGTGTCGCAAGCCC GGAAGCGGCCGCTGAACCCTTGAAGCAGTTGTTGCGCCCAGGTGATCATGTGCTGCTGAAAGCCAGCCGAGGGATTGCCT TGGAGCGGTTGATACCTCTGCTTGAGACAGGCTGA
Upstream 100 bases:
>100_bases TCGGTTACGACAACCTGCAGCTCGTTGATGCCACTGGCAAGCTTGTGGGGCGTTCAGCCCAGGTTGGCGAGGGCATGATT CTTTGGAACCTGGCCAACCC
Downstream 100 bases:
>100_bases TGGACGCGTTGTTTGGTCCATTCATTCGATCACACATGAGGTGGTGATTGAACTCAACCCAATTACACTGATTGGAGCTA ATCAGCAAGTGTGATCAAGC
Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alani ne ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 464; Mature: 463
Protein sequence:
>464_residues MSPSLCLQDLARLLDAPVQRRSAPADPTTLAWTFEHISTDSRALQPGDLFIPLRGERFDGHDFLDAAVSVGVAGALIARD WAGYVPEQLATVVVDDTLSAYQQIALAWRRQLHCPVVAVTGSAGKTTTRELIKACLSPLGAIESSVGNENNDIGVPRTML RSNSSHAGLVLEMGMRGLGEIHRLSVCAEPDVAVITNIGTAHIGRLGSRAAIAQAKCEITDRLKPSGLLVIPAGDPLLEA EVSRVWSGRVQRVALSDDPLEPGTPAPDWVAQLQGQSIELASQGLKLINPLEGRHNARNFLLALAAAQELGVTAEQLRDL SVSLPGGRARRLRINGVNILDETYNASPEAMLASLELLKQQPGGHRYAVVGTMLELGDQSLSLHRQVGEKATALGLDGLV VVASGPEASALMQGAAGLSKLVCVASPEAAAEPLKQLLRPGDHVLLKASRGIALERLIPLLETG
Sequences:
>Translated_464_residues MSPSLCLQDLARLLDAPVQRRSAPADPTTLAWTFEHISTDSRALQPGDLFIPLRGERFDGHDFLDAAVSVGVAGALIARD WAGYVPEQLATVVVDDTLSAYQQIALAWRRQLHCPVVAVTGSAGKTTTRELIKACLSPLGAIESSVGNENNDIGVPRTML RSNSSHAGLVLEMGMRGLGEIHRLSVCAEPDVAVITNIGTAHIGRLGSRAAIAQAKCEITDRLKPSGLLVIPAGDPLLEA EVSRVWSGRVQRVALSDDPLEPGTPAPDWVAQLQGQSIELASQGLKLINPLEGRHNARNFLLALAAAQELGVTAEQLRDL SVSLPGGRARRLRINGVNILDETYNASPEAMLASLELLKQQPGGHRYAVVGTMLELGDQSLSLHRQVGEKATALGLDGLV VVASGPEASALMQGAAGLSKLVCVASPEAAAEPLKQLLRPGDHVLLKASRGIALERLIPLLETG >Mature_463_residues SPSLCLQDLARLLDAPVQRRSAPADPTTLAWTFEHISTDSRALQPGDLFIPLRGERFDGHDFLDAAVSVGVAGALIARDW AGYVPEQLATVVVDDTLSAYQQIALAWRRQLHCPVVAVTGSAGKTTTRELIKACLSPLGAIESSVGNENNDIGVPRTMLR SNSSHAGLVLEMGMRGLGEIHRLSVCAEPDVAVITNIGTAHIGRLGSRAAIAQAKCEITDRLKPSGLLVIPAGDPLLEAE VSRVWSGRVQRVALSDDPLEPGTPAPDWVAQLQGQSIELASQGLKLINPLEGRHNARNFLLALAAAQELGVTAEQLRDLS VSLPGGRARRLRINGVNILDETYNASPEAMLASLELLKQQPGGHRYAVVGTMLELGDQSLSLHRQVGEKATALGLDGLVV VASGPEASALMQGAAGLSKLVCVASPEAAAEPLKQLLRPGDHVLLKASRGIALERLIPLLETG
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=442, Percent_Identity=33.2579185520362, Blast_Score=179, Evalue=3e-46,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 49070; Mature: 48939
Theoretical pI: Translated: 6.02; Mature: 6.02
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 1.3 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSPSLCLQDLARLLDAPVQRRSAPADPTTLAWTFEHISTDSRALQPGDLFIPLRGERFDG CCHHHHHHHHHHHHHCHHHHCCCCCCCCEEEEEEHHHCCCCCCCCCCCEEEEECCCCCCC HDFLDAAVSVGVAGALIARDWAGYVPEQLATVVVDDTLSAYQQIALAWRRQLHCPVVAVT HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE GSAGKTTTRELIKACLSPLGAIESSVGNENNDIGVPRTMLRSNSSHAGLVLEMGMRGLGE CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCEEEECCCHHHCH IHRLSVCAEPDVAVITNIGTAHIGRLGSRAAIAQAKCEITDRLKPSGLLVIPAGDPLLEA HHHEEECCCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH EVSRVWSGRVQRVALSDDPLEPGTPAPDWVAQLQGQSIELASQGLKLINPLEGRHNARNF HHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHCCHHHHCCCCCCCHHHHH LLALAAAQELGVTAEQLRDLSVSLPGGRARRLRINGVNILDETYNASPEAMLASLELLKQ HHHHHHHHHHCCCHHHHHCCEEECCCCCEEEEEECCCEEEHHCCCCCHHHHHHHHHHHHC QPGGHRYAVVGTMLELGDQSLSLHRQVGEKATALGLDGLVVVASGPEASALMQGAAGLSK CCCCCEEEHHHHHHHHCCHHHHHHHHHCCHHHHCCCCCEEEEECCCCHHHHHHHHCCHHH LVCVASPEAAAEPLKQLLRPGDHVLLKASRGIALERLIPLLETG EEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCC >Mature Secondary Structure SPSLCLQDLARLLDAPVQRRSAPADPTTLAWTFEHISTDSRALQPGDLFIPLRGERFDG CHHHHHHHHHHHHHCHHHHCCCCCCCCEEEEEEHHHCCCCCCCCCCCEEEEECCCCCCC HDFLDAAVSVGVAGALIARDWAGYVPEQLATVVVDDTLSAYQQIALAWRRQLHCPVVAVT HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE GSAGKTTTRELIKACLSPLGAIESSVGNENNDIGVPRTMLRSNSSHAGLVLEMGMRGLGE CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCEEEECCCHHHCH IHRLSVCAEPDVAVITNIGTAHIGRLGSRAAIAQAKCEITDRLKPSGLLVIPAGDPLLEA HHHEEECCCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH EVSRVWSGRVQRVALSDDPLEPGTPAPDWVAQLQGQSIELASQGLKLINPLEGRHNARNF HHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHCCHHHHCCCCCCCHHHHH LLALAAAQELGVTAEQLRDLSVSLPGGRARRLRINGVNILDETYNASPEAMLASLELLKQ HHHHHHHHHHCCCHHHHHCCEEECCCCCEEEEEECCCEEEHHCCCCCHHHHHHHHHHHHC QPGGHRYAVVGTMLELGDQSLSLHRQVGEKATALGLDGLVVVASGPEASALMQGAAGLSK CCCCCEEEHHHHHHHHCCHHHHHHHHHCCHHHHCCCCCEEEEECCCCHHHHHHHHCCHHH LVCVASPEAAAEPLKQLLRPGDHVLLKASRGIALERLIPLLETG EEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7894708; 8905231 [H]