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The map label for this gene is purA

Identifier: 148241978

GI number: 148241978

Start: 797267

End: 798580

Strand: Direct

Name: purA

Synonym: SynRCC307_0879

Alternate gene names: 148241978

Gene position: 797267-798580 (Clockwise)

Preceding gene: 148241977

Following gene: 148241979

Centisome position: 35.83

GC content: 62.02

Gene sequence:

>1314_bases
TTGGCCAACGTTGTCGTCATCGGTGCTCAATGGGGTGACGAGGGCAAGGGAAAGATCACCGATCTGCTCAGCCGCTCTGC
CGACGTCGTCGTGCGTTATCAGGGTGGTGTCAACGCCGGGCACACCATCGTTGTCGATGGTCAAGTGCTCAAGCTGCACC
TGATCCCCTCTGGAATCCTTTACCCCGACACCACCTGCCTGATCGGATCCGGCACGGTGGTCGATCCCAAGGTTTTGCTG
GGCGAGCTGGCGATGCTCGCCAGCAACGACATTGACGCCAGCGGCTTGAAGGTGGCCTCCACCGCCCATGTGACGCTCCC
GTATCACCGCCTGCTCGATCAGGCGATGGAGCAACAACGCGGCAACCAGCGCATTGGCACCACTGGCCGTGGCATCGGCC
CCACCTATTCCGATAAGGCTGAGCGCAGTGGTCTGCGCATGATCGATCTGCTCGATGAGCAGCTGCTGCGCGAGCGACTG
CAGGGGCCGATCGCTGCGAAAAACCTGCAGCTGCAGAAGCTGTATGGCCTTGAGCCCCTCGATGCCGAGGAGGTGATTGC
CGAATACGCCGACTACGGCCGCCGCCTCTCCAGCCATGTGGTGGATTGCACCCGCGCCATCCATGACGCCGCGCGGGCAC
GCAAAAACATTCTGTTTGAAGGGGCCCAAGGCACCCTGCTGGATCTCGATCACGGCACCTATCCCTATGTGACGTCCTCC
AACCCTGTGGCTGGTGGTGCCTGCATTGGTGCTGGGGTTGGCCCAACCCTGATCGACCGGGTGATCGGAGTTGCCAAGGC
CTACACCACCCGCGTCGGTGAGGGGCCCTTTCCCACTGAGTTGGAGGGCAGCCTCTCGGATCACCTTTGCGATCGCGGCG
GCGAGTACGGCACCACCACGGGCCGCCGGCGCCGCTGTGGCTGGTTTGACGGGGTGATCGGCCGCTATGCGGTGGAGGTC
AACGGCTTGGATTGCCTGGCCATCACCAAGCTGGATGTGCTCGATGAGCTCGATGAAATCCAGGTCTGTGTGGCCTATGA
ACTCGATGGTCAGCGGGTGAATCATTTCCCCAGCAGCGCCTCGGAGTTCGCCCGTTGCCAGCCAGTGTTTGAAACCCTGC
CTGGCTGGCAAACCTCCACCGCGGATTGCCGCAGCCTTGATGACTTGCCGGAGAAGGCGATGTCGTACTTGCGCTTTTTG
GCAGATCTGATGGAGGTGCCCATCGCGATCGTGTCCCTGGGTGCCAGCCGCGATCAAACCATCGTGGTGGAGGATCCCAT
CCACGGACCCAAACGAGCACTGCTCAGCGCATGA

Upstream 100 bases:

>100_bases
GAGCTCAGTGTCGTCCGCGGCAGCTGATTTTTTGACGCGGGGGTGGTTGATCTGAAAAAATCGCGGATTGTTCGGATTGC
GGCCCTGAGTCGCCCTTGCT

Downstream 100 bases:

>100_bases
CCACCACCCCTTCAGCTCTCGATGTCGTTGGCATCGGCAACGCCATCGTTGATGTGCTCTCTGAGAGTGACGACGGTTTT
CTCGATCGCCAGGGCCTGAC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDGQVLKLHLIPSGILYPDTTCLIGSGTVVDPKVLL
GELAMLASNDIDASGLKVASTAHVTLPYHRLLDQAMEQQRGNQRIGTTGRGIGPTYSDKAERSGLRMIDLLDEQLLRERL
QGPIAAKNLQLQKLYGLEPLDAEEVIAEYADYGRRLSSHVVDCTRAIHDAARARKNILFEGAQGTLLDLDHGTYPYVTSS
NPVAGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELEGSLSDHLCDRGGEYGTTTGRRRRCGWFDGVIGRYAVEV
NGLDCLAITKLDVLDELDEIQVCVAYELDGQRVNHFPSSASEFARCQPVFETLPGWQTSTADCRSLDDLPEKAMSYLRFL
ADLMEVPIAIVSLGASRDQTIVVEDPIHGPKRALLSA

Sequences:

>Translated_437_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDGQVLKLHLIPSGILYPDTTCLIGSGTVVDPKVLL
GELAMLASNDIDASGLKVASTAHVTLPYHRLLDQAMEQQRGNQRIGTTGRGIGPTYSDKAERSGLRMIDLLDEQLLRERL
QGPIAAKNLQLQKLYGLEPLDAEEVIAEYADYGRRLSSHVVDCTRAIHDAARARKNILFEGAQGTLLDLDHGTYPYVTSS
NPVAGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELEGSLSDHLCDRGGEYGTTTGRRRRCGWFDGVIGRYAVEV
NGLDCLAITKLDVLDELDEIQVCVAYELDGQRVNHFPSSASEFARCQPVFETLPGWQTSTADCRSLDDLPEKAMSYLRFL
ADLMEVPIAIVSLGASRDQTIVVEDPIHGPKRALLSA
>Mature_436_residues
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDGQVLKLHLIPSGILYPDTTCLIGSGTVVDPKVLLG
ELAMLASNDIDASGLKVASTAHVTLPYHRLLDQAMEQQRGNQRIGTTGRGIGPTYSDKAERSGLRMIDLLDEQLLRERLQ
GPIAAKNLQLQKLYGLEPLDAEEVIAEYADYGRRLSSHVVDCTRAIHDAARARKNILFEGAQGTLLDLDHGTYPYVTSSN
PVAGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELEGSLSDHLCDRGGEYGTTTGRRRRCGWFDGVIGRYAVEVN
GLDCLAITKLDVLDELDEIQVCVAYELDGQRVNHFPSSASEFARCQPVFETLPGWQTSTADCRSLDDLPEKAMSYLRFLA
DLMEVPIAIVSLGASRDQTIVVEDPIHGPKRALLSA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=43.6619718309859, Blast_Score=349, Evalue=3e-96,
Organism=Homo sapiens, GI22748717, Length=431, Percent_Identity=44.0835266821346, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI40316944, Length=402, Percent_Identity=42.2885572139304, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=48.5981308411215, Blast_Score=407, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=41.8224299065421, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=41.8224299065421, Blast_Score=329, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=42.5925925925926, Blast_Score=344, Evalue=2e-95,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=43.3962264150943, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_SYNR3 (A5GSC3)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001227135.1
- ProteinModelPortal:   A5GSC3
- SMR:   A5GSC3
- STRING:   A5GSC3
- GeneID:   5158779
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_0879
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47108; Mature: 46977

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDGQVLKLHLIPSGIL
CCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEECCEEEEEEECCCCCC
YPDTTCLIGSGTVVDPKVLLGELAMLASNDIDASGLKVASTAHVTLPYHRLLDQAMEQQR
CCCCEEEECCCCEECHHHHHHHHHHHHCCCCCCCCEEEECEEEEECCHHHHHHHHHHHHC
GNQRIGTTGRGIGPTYSDKAERSGLRMIDLLDEQLLRERLQGPIAAKNLQLQKLYGLEPL
CCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCC
DAEEVIAEYADYGRRLSSHVVDCTRAIHDAARARKNILFEGAQGTLLDLDHGTYPYVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCEEECC
NPVAGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELEGSLSDHLCDRGGEYGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
GRRRRCGWFDGVIGRYAVEVNGLDCLAITKLDVLDELDEIQVCVAYELDGQRVNHFPSSA
CCCCCCCHHHHHHHEEEEEECCCEEEEEHHHHHHHHHHHEEEEEEEECCCCHHHCCCCCH
SEFARCQPVFETLPGWQTSTADCRSLDDLPEKAMSYLRFLADLMEVPIAIVSLGASRDQT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IVVEDPIHGPKRALLSA
EEEECCCCCHHHHHCCC
>Mature Secondary Structure 
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDGQVLKLHLIPSGIL
CCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEECCEEEEEEECCCCCC
YPDTTCLIGSGTVVDPKVLLGELAMLASNDIDASGLKVASTAHVTLPYHRLLDQAMEQQR
CCCCEEEECCCCEECHHHHHHHHHHHHCCCCCCCCEEEECEEEEECCHHHHHHHHHHHHC
GNQRIGTTGRGIGPTYSDKAERSGLRMIDLLDEQLLRERLQGPIAAKNLQLQKLYGLEPL
CCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCC
DAEEVIAEYADYGRRLSSHVVDCTRAIHDAARARKNILFEGAQGTLLDLDHGTYPYVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCEEECC
NPVAGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELEGSLSDHLCDRGGEYGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
GRRRRCGWFDGVIGRYAVEVNGLDCLAITKLDVLDELDEIQVCVAYELDGQRVNHFPSSA
CCCCCCCHHHHHHHEEEEEECCCEEEEEHHHHHHHHHHHEEEEEEEECCCCHHHCCCCCH
SEFARCQPVFETLPGWQTSTADCRSLDDLPEKAMSYLRFLADLMEVPIAIVSLGASRDQT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IVVEDPIHGPKRALLSA
EEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA