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The map label for this gene is proS

Identifier: 148241975

GI number: 148241975

Start: 794514

End: 796316

Strand: Direct

Name: proS

Synonym: SynRCC307_0876

Alternate gene names: 148241975

Gene position: 794514-796316 (Clockwise)

Preceding gene: 148241971

Following gene: 148241976

Centisome position: 35.71

GC content: 61.9

Gene sequence:

>1803_bases
ATGCGCGTCTCCGCCCTGTTGTTGGTGACGCTGCGCGATGACCCGGCAGAAGCGGAAATTCCTTCCCACAAACTGCTGTT
GCGTGGCGGCTATATCCGCCGTGTGGCCTCGGGGATTTACGCCTATCTGCCTTTGATGTGGCGCGTGCTGCGCAAGGTCT
CGGCGATCGTTCGCCAAGAAATGGATGCCGCCGGTGCCCTCGAAACCCTGCTGCCCCAGCTGCAACCCGCTGAGCTTTGG
CAGCGCAGTGGTCGTTGGTCGGGCTACACCGCTGGGGAAGGAATCATGTTTCACCTGCAGGACCGCCAGGACCGGGAGCT
GGGGCTTGGCCCCACCCACGAAGAGGTGATCACAGCGCTGGCTGCCGACCTGCTGCGCTCCTACCGCCAGCTCCCCGTCA
ACCTCTATCAGATCCAAACGAAATTCAGAGATGAAATCCGCCCGCGCTTCGGCTTGATGCGTGGCCGCGAATTCATCATG
AAAGATGCCTATTCCTTCCATGCCAGCGCTGAAGATCTCGGCCGCGGCTATGCCGCCATGGATCAGGCCTATCGCCGCAT
CTTTTCCCGCTGCGGTCTTCAGGTTGTGGCTGTTCAAGCCGACAGCGGTGCCATCGGTGGTTCCGCTAGCCAGGAGTTCA
TGGTCACCGCTGAAGCCGGTGAAGACCTCATTCTCAGCAGTGCGGATGGCTCCTATGCGGCCAACCAAGAGCGGGCCGAA
TCCCTGCCGGCTGAGCCGGTGCCCCTGCAGAGCGCGACAGCCGCTGAGCTGGACACCCCCGGGCAAACCAGCATTGATGC
CTTGGTGGAGGCCCAGGGCTGGCACGCCTCTCAGTTGGTCAAAGTGATCCTGTTGGTGGCGCGCTTTGAGCAAGGTCGCC
AGCAGCCCCTGCTGGTCTCTCTGCGCGGTGATCAACAGCTCAATGAAGTGACGCTGGCCAATTGGCTCAATCAGCAGCAC
GGCCAGGCTTGGGGTGCTCTCTTAGGCATTGAGCCGTTGGAGGCCAAGCATCTGGCAGCTGAAAAAGTGCCTGCCTTTGG
CTACCTCGGCCCTGATCTCAGTGATGCGGTTTTGCAGGGCAGCAAAAAATTGGAGAGCAGCTTTTTGCGTTTGGCCGATC
CAACGGCCCTGGATTTGCCCCTGTTCATTTGCGGAGCCAACCGCTTCAATGCCCATCGCCTCGCCGCTAATTGGAGCGAG
CCCGGCATGGCCAGCCCTCAGCGCGTCGAGCTGCGCGCCGCGCTGCCCGGTGATCGCTGTTGCCATGACCCCAGCCAACA
GCTGCAGGCCCGCCGCGGCATTGAAGTGGGCCACATCTTCCAGCTGGGGCTCAAGTACTCCGAGGCCCTCGGCGCCACCT
TTGCCAACGAGCAGGGGCAAGACGCCCCCCTTTGGATGGGTTGCTACGGCATTGGCGTGTCGCGCTTGGCCCAAGCCGCC
GTTGAGCAGCACCATGACTCCGCCGGCATGGTGTGGCCTGTGCCCATCGCACCGTTTGAAGTGGTGATCGTGATCGCCAG
CAGCAAAGAAGCGCAGCAGGTGGAGTTGGCCGAAGATCTTTATGGGCAGTTGCAGCAGGCTGGCGTCGATGTGCTGCTCG
ATGACCGCAACGAGCGGGCCGGCGTGAAATTCAAAGACGCCGAACTCATCGGCATTCCTTGGCGCCTGGTGGTGGGACGC
GGTGCTGTCAATGGCCAGGTGGAGCTGGTGGAGCGCTGCAGTGGCGAGAAGCAGGAGGGTCCCCACCAAGACTTGATGGC
TCAGTTGCTGCAGACCCTGGATCAACAACGTCAGGGGTTGTGA

Upstream 100 bases:

>100_bases
AGCCTGTGGAGGTTAGTGAATCGTCCCCATGAGACTCAATTTACTTTCTTCGTCTTCAATGCCTCGGTGATAGGTTCCGC
CACCTGTTCTGCTGCAAGCG

Downstream 100 bases:

>100_bases
TGAGACGCTCTTTGGCTGCTGCTGCTGCTGCCCTCGCGGCTGTGCTGGTGGGGTGCCGTGCCGCTCCCCCGGTGCCTCCG
ATGACCGCCGAGCGACGTTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MRVSALLLVTLRDDPAEAEIPSHKLLLRGGYIRRVASGIYAYLPLMWRVLRKVSAIVRQEMDAAGALETLLPQLQPAELW
QRSGRWSGYTAGEGIMFHLQDRQDRELGLGPTHEEVITALAADLLRSYRQLPVNLYQIQTKFRDEIRPRFGLMRGREFIM
KDAYSFHASAEDLGRGYAAMDQAYRRIFSRCGLQVVAVQADSGAIGGSASQEFMVTAEAGEDLILSSADGSYAANQERAE
SLPAEPVPLQSATAAELDTPGQTSIDALVEAQGWHASQLVKVILLVARFEQGRQQPLLVSLRGDQQLNEVTLANWLNQQH
GQAWGALLGIEPLEAKHLAAEKVPAFGYLGPDLSDAVLQGSKKLESSFLRLADPTALDLPLFICGANRFNAHRLAANWSE
PGMASPQRVELRAALPGDRCCHDPSQQLQARRGIEVGHIFQLGLKYSEALGATFANEQGQDAPLWMGCYGIGVSRLAQAA
VEQHHDSAGMVWPVPIAPFEVVIVIASSKEAQQVELAEDLYGQLQQAGVDVLLDDRNERAGVKFKDAELIGIPWRLVVGR
GAVNGQVELVERCSGEKQEGPHQDLMAQLLQTLDQQRQGL

Sequences:

>Translated_600_residues
MRVSALLLVTLRDDPAEAEIPSHKLLLRGGYIRRVASGIYAYLPLMWRVLRKVSAIVRQEMDAAGALETLLPQLQPAELW
QRSGRWSGYTAGEGIMFHLQDRQDRELGLGPTHEEVITALAADLLRSYRQLPVNLYQIQTKFRDEIRPRFGLMRGREFIM
KDAYSFHASAEDLGRGYAAMDQAYRRIFSRCGLQVVAVQADSGAIGGSASQEFMVTAEAGEDLILSSADGSYAANQERAE
SLPAEPVPLQSATAAELDTPGQTSIDALVEAQGWHASQLVKVILLVARFEQGRQQPLLVSLRGDQQLNEVTLANWLNQQH
GQAWGALLGIEPLEAKHLAAEKVPAFGYLGPDLSDAVLQGSKKLESSFLRLADPTALDLPLFICGANRFNAHRLAANWSE
PGMASPQRVELRAALPGDRCCHDPSQQLQARRGIEVGHIFQLGLKYSEALGATFANEQGQDAPLWMGCYGIGVSRLAQAA
VEQHHDSAGMVWPVPIAPFEVVIVIASSKEAQQVELAEDLYGQLQQAGVDVLLDDRNERAGVKFKDAELIGIPWRLVVGR
GAVNGQVELVERCSGEKQEGPHQDLMAQLLQTLDQQRQGL
>Mature_600_residues
MRVSALLLVTLRDDPAEAEIPSHKLLLRGGYIRRVASGIYAYLPLMWRVLRKVSAIVRQEMDAAGALETLLPQLQPAELW
QRSGRWSGYTAGEGIMFHLQDRQDRELGLGPTHEEVITALAADLLRSYRQLPVNLYQIQTKFRDEIRPRFGLMRGREFIM
KDAYSFHASAEDLGRGYAAMDQAYRRIFSRCGLQVVAVQADSGAIGGSASQEFMVTAEAGEDLILSSADGSYAANQERAE
SLPAEPVPLQSATAAELDTPGQTSIDALVEAQGWHASQLVKVILLVARFEQGRQQPLLVSLRGDQQLNEVTLANWLNQQH
GQAWGALLGIEPLEAKHLAAEKVPAFGYLGPDLSDAVLQGSKKLESSFLRLADPTALDLPLFICGANRFNAHRLAANWSE
PGMASPQRVELRAALPGDRCCHDPSQQLQARRGIEVGHIFQLGLKYSEALGATFANEQGQDAPLWMGCYGIGVSRLAQAA
VEQHHDSAGMVWPVPIAPFEVVIVIASSKEAQQVELAEDLYGQLQQAGVDVLLDDRNERAGVKFKDAELIGIPWRLVVGR
GAVNGQVELVERCSGEKQEGPHQDLMAQLLQTLDQQRQGL

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=255, Percent_Identity=41.5686274509804, Blast_Score=192, Evalue=8e-49,
Organism=Escherichia coli, GI1786392, Length=597, Percent_Identity=46.2311557788945, Blast_Score=465, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI115532348, Length=259, Percent_Identity=37.8378378378378, Blast_Score=158, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6320931, Length=560, Percent_Identity=30.8928571428571, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24656200, Length=237, Percent_Identity=37.1308016877637, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_SYNR3 (A5GSC0)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001227132.1
- ProteinModelPortal:   A5GSC0
- SMR:   A5GSC0
- STRING:   A5GSC0
- GeneID:   5158762
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_0876
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 65808; Mature: 65808

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSALLLVTLRDDPAEAEIPSHKLLLRGGYIRRVASGIYAYLPLMWRVLRKVSAIVRQE
CCCEEEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDAAGALETLLPQLQPAELWQRSGRWSGYTAGEGIMFHLQDRQDRELGLGPTHEEVITAL
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHHH
AADLLRSYRQLPVNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFHASAEDLGRGYAAM
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHH
DQAYRRIFSRCGLQVVAVQADSGAIGGSASQEFMVTAEAGEDLILSSADGSYAANQERAE
HHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHH
SLPAEPVPLQSATAAELDTPGQTSIDALVEAQGWHASQLVKVILLVARFEQGRQQPLLVS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
LRGDQQLNEVTLANWLNQQHGQAWGALLGIEPLEAKHLAAEKVPAFGYLGPDLSDAVLQG
ECCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHC
SKKLESSFLRLADPTALDLPLFICGANRFNAHRLAANWSEPGMASPQRVELRAALPGDRC
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHEECCCCCCCCCCCCEEEEEECCCCCCC
CHDPSQQLQARRGIEVGHIFQLGLKYSEALGATFANEQGQDAPLWMGCYGIGVSRLAQAA
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEHHHHHHHHHHHHHH
VEQHHDSAGMVWPVPIAPFEVVIVIASSKEAQQVELAEDLYGQLQQAGVDVLLDDRNERA
HHHCCCCCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
GVKFKDAELIGIPWRLVVGRGAVNGQVELVERCSGEKQEGPHQDLMAQLLQTLDQQRQGL
CCEEECCEEECCCEEEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVSALLLVTLRDDPAEAEIPSHKLLLRGGYIRRVASGIYAYLPLMWRVLRKVSAIVRQE
CCCEEEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDAAGALETLLPQLQPAELWQRSGRWSGYTAGEGIMFHLQDRQDRELGLGPTHEEVITAL
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHHH
AADLLRSYRQLPVNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFHASAEDLGRGYAAM
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHH
DQAYRRIFSRCGLQVVAVQADSGAIGGSASQEFMVTAEAGEDLILSSADGSYAANQERAE
HHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHH
SLPAEPVPLQSATAAELDTPGQTSIDALVEAQGWHASQLVKVILLVARFEQGRQQPLLVS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
LRGDQQLNEVTLANWLNQQHGQAWGALLGIEPLEAKHLAAEKVPAFGYLGPDLSDAVLQG
ECCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHC
SKKLESSFLRLADPTALDLPLFICGANRFNAHRLAANWSEPGMASPQRVELRAALPGDRC
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHEECCCCCCCCCCCCEEEEEECCCCCCC
CHDPSQQLQARRGIEVGHIFQLGLKYSEALGATFANEQGQDAPLWMGCYGIGVSRLAQAA
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEHHHHHHHHHHHHHH
VEQHHDSAGMVWPVPIAPFEVVIVIASSKEAQQVELAEDLYGQLQQAGVDVLLDDRNERA
HHHCCCCCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
GVKFKDAELIGIPWRLVVGRGAVNGQVELVERCSGEKQEGPHQDLMAQLLQTLDQQRQGL
CCEEECCEEECCCEEEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA