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The map label for this gene is leuA

Identifier: 148241845

GI number: 148241845

Start: 674155

End: 675768

Strand: Direct

Name: leuA

Synonym: SynRCC307_0746

Alternate gene names: 148241845

Gene position: 674155-675768 (Clockwise)

Preceding gene: 148241842

Following gene: 148241846

Centisome position: 30.3

GC content: 60.9

Gene sequence:

>1614_bases
ATGGCGCGCGACCCGGGCCGGGTGTTGATTTTCGACACCACCCTCAGGGATGGGGAGCAGTCGCCTGGGGCCAGCCTGAA
TCTGGATGAAAAGCTGGCCATCGCTCAGCAACTGGCCCGCCTGCGCGTCGACATCATCGAGGCTGGGTTTCCCTTTGCCA
GTCCCGGTGACTTTGATGCGGTGCAAACCATTGCTCGCCAGGTTGGTCGCCCCGATGGACCGGTGATTTGTGGCTTAGCT
CGCGCCACGCGCGGCGACATCAAAGCCTGCGCTGATGCGGTGGCACCGGCTGCCAACCAGCGCATCCATACCTTTCTCGC
CACCAGCGACATTCACCTCGAGCACAAGTTGCGCAAGAGTCGCGCTGAGGTGCTGCAGATCGTTCCGGAGATGGTGGCCT
ATGCCCGCTCCTTGGTGGACGACGTGGAGTTTTCCTGTGAGGACGCCGGGCGCAGCGATCCCGAGTTCATGTATCAGGTG
ATCGAAGCGGCCATCGAGGCTGGTGCCACCACCATCAACATTCCCGACACGGTCGGCTACTCCACGCCCGCTGAATTTGG
TGCCTTGATTGCTGGCATCGATGCCCACGTGCCCAACATCGGCCAAGCGGTGATCTCCGTGCATGGCCATAACGACTTGG
GTCTGGCCGTGGCCAACTTCCTCGAGGCCGTCAAAAATGGCGCCCGGCAGCTGGAGTGCACGATCAATGGCATCGGTGAG
CGCGCTGGTAATGCATCGCTTGAAGAGCTGGTGATGGCGCTGCATGTGCGCCGTAGCTACTTCAACGGCTACCTCGGCCG
AGCTGAAGACAGCAGCGAGCCTTTAACAGGCATCCAAACCGAGGAGATTTACAAAACCTCTCGCCTGGTCTCCAACCTCA
CCGGCATGGCGGTGCAGCCCAACAAAGCCATCGTGGGTGCCAATGCCTTTGCCCATGAATCCGGCATCCACCAAGACGGT
GTGCTGAAGAACCGCCTCACCTACGAAATCATTGATGCCCGAACCATCGGCTTGACCGATAACCGCATCAGCCTGGGCAA
GCTCAGTGGTCGCAGTGCCGTGCGCGCACGCCTTGAGGAGCTGGGGTATCAGCTCGATGGCGATGACCTCAACGATGCGT
TCGCTCGCTTTAAAGAACTCGCTGATCGCAAGCGAGAGATCACCGACCGCGATCTAGAGGCCATCGTTCGGCAGAACGCC
CAGCAGATCGAGGCCTACTACCAGCTGGCCGGAGTGCAGGTGAGTTGTGGGCGAGATCTGCGCGCAACAGCCACGGTCAC
CTTGCGCACCAGTGATGGTGAGGAGTGCTCCCAAGCTGCCATTGGCACCGGGCCTGTTGATGCCGTTTGCCAGGCCCTCA
ATGGCTTGGTTCAAGTCCCCAATGAACTGGTGGAATTCAGCGTGAAATCCGTCACCGAAGGCATCGATGCCATGGGTGAG
GTGACGATTCGCCTGCGCCAGGACGGCCGTTTGTATTCCGGTCATGCCGCTGACACCGATGTGGTGGTGGCCGCTGCCCA
GGCGTTTGTGAATGCCCTCAACCGCTTGGTGAGCGGCCAGAAACACAGCCCGCTGCATCCCCAACGCGCACCCCTGCCGG
CCCCAGCGCTATGA

Upstream 100 bases:

>100_bases
CATGAAACGCTGTCAACTGCTGCACTGTACGGCGATTTTCTTGAACAAAGGCCGTGAGGCTTAGAATGGAGCTAACCCGA
AATCAATCCGCATAAGACGT

Downstream 100 bases:

>100_bases
CCGCTCGCCGCGCGCCTCTGTGGTCGCTGCTGCTGCAGCTGACGGTGCTGTTGCTGGTGGCCTTGGTGGTTTTGATCCCC
TTGCTCTGGCTGCTGAGCAC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MARDPGRVLIFDTTLRDGEQSPGASLNLDEKLAIAQQLARLRVDIIEAGFPFASPGDFDAVQTIARQVGRPDGPVICGLA
RATRGDIKACADAVAPAANQRIHTFLATSDIHLEHKLRKSRAEVLQIVPEMVAYARSLVDDVEFSCEDAGRSDPEFMYQV
IEAAIEAGATTINIPDTVGYSTPAEFGALIAGIDAHVPNIGQAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNASLEELVMALHVRRSYFNGYLGRAEDSSEPLTGIQTEEIYKTSRLVSNLTGMAVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDARTIGLTDNRISLGKLSGRSAVRARLEELGYQLDGDDLNDAFARFKELADRKREITDRDLEAIVRQNA
QQIEAYYQLAGVQVSCGRDLRATATVTLRTSDGEECSQAAIGTGPVDAVCQALNGLVQVPNELVEFSVKSVTEGIDAMGE
VTIRLRQDGRLYSGHAADTDVVVAAAQAFVNALNRLVSGQKHSPLHPQRAPLPAPAL

Sequences:

>Translated_537_residues
MARDPGRVLIFDTTLRDGEQSPGASLNLDEKLAIAQQLARLRVDIIEAGFPFASPGDFDAVQTIARQVGRPDGPVICGLA
RATRGDIKACADAVAPAANQRIHTFLATSDIHLEHKLRKSRAEVLQIVPEMVAYARSLVDDVEFSCEDAGRSDPEFMYQV
IEAAIEAGATTINIPDTVGYSTPAEFGALIAGIDAHVPNIGQAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNASLEELVMALHVRRSYFNGYLGRAEDSSEPLTGIQTEEIYKTSRLVSNLTGMAVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDARTIGLTDNRISLGKLSGRSAVRARLEELGYQLDGDDLNDAFARFKELADRKREITDRDLEAIVRQNA
QQIEAYYQLAGVQVSCGRDLRATATVTLRTSDGEECSQAAIGTGPVDAVCQALNGLVQVPNELVEFSVKSVTEGIDAMGE
VTIRLRQDGRLYSGHAADTDVVVAAAQAFVNALNRLVSGQKHSPLHPQRAPLPAPAL
>Mature_536_residues
ARDPGRVLIFDTTLRDGEQSPGASLNLDEKLAIAQQLARLRVDIIEAGFPFASPGDFDAVQTIARQVGRPDGPVICGLAR
ATRGDIKACADAVAPAANQRIHTFLATSDIHLEHKLRKSRAEVLQIVPEMVAYARSLVDDVEFSCEDAGRSDPEFMYQVI
EAAIEAGATTINIPDTVGYSTPAEFGALIAGIDAHVPNIGQAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGER
AGNASLEELVMALHVRRSYFNGYLGRAEDSSEPLTGIQTEEIYKTSRLVSNLTGMAVQPNKAIVGANAFAHESGIHQDGV
LKNRLTYEIIDARTIGLTDNRISLGKLSGRSAVRARLEELGYQLDGDDLNDAFARFKELADRKREITDRDLEAIVRQNAQ
QIEAYYQLAGVQVSCGRDLRATATVTLRTSDGEECSQAAIGTGPVDAVCQALNGLVQVPNELVEFSVKSVTEGIDAMGEV
TIRLRQDGRLYSGHAADTDVVVAAAQAFVNALNRLVSGQKHSPLHPQRAPLPAPAL

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=512, Percent_Identity=49.21875, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320019, Length=400, Percent_Identity=27, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=399, Percent_Identity=27.0676691729323, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6324682, Length=505, Percent_Identity=25.3465346534653, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324225, Length=261, Percent_Identity=30.2681992337165, Blast_Score=100, Evalue=6e-22,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_SYNR3 (A5GRZ0)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001227002.1
- ProteinModelPortal:   A5GRZ0
- SMR:   A5GRZ0
- STRING:   A5GRZ0
- GeneID:   5157699
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_0746
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 57644; Mature: 57512

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDPGRVLIFDTTLRDGEQSPGASLNLDEKLAIAQQLARLRVDIIEAGFPFASPGDFDA
CCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VQTIARQVGRPDGPVICGLARATRGDIKACADAVAPAANQRIHTFLATSDIHLEHKLRKS
HHHHHHHCCCCCCCEEEEHHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHH
RAEVLQIVPEMVAYARSLVDDVEFSCEDAGRSDPEFMYQVIEAAIEAGATTINIPDTVGY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
STPAEFGALIAGIDAHVPNIGQAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECHHC
RAGNASLEELVMALHVRRSYFNGYLGRAEDSSEPLTGIQTEEIYKTSRLVSNLTGMAVQP
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDARTIGLTDNRISLGKLSGRSAVRARLEE
CCEEEECCHHHHHCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
LGYQLDGDDLNDAFARFKELADRKREITDRDLEAIVRQNAQQIEAYYQLAGVQVSCGRDL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCEEEECCCCC
RATATVTLRTSDGEECSQAAIGTGPVDAVCQALNGLVQVPNELVEFSVKSVTEGIDAMGE
EEEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
VTIRLRQDGRLYSGHAADTDVVVAAAQAFVNALNRLVSGQKHSPLHPQRAPLPAPAL
EEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARDPGRVLIFDTTLRDGEQSPGASLNLDEKLAIAQQLARLRVDIIEAGFPFASPGDFDA
CCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VQTIARQVGRPDGPVICGLARATRGDIKACADAVAPAANQRIHTFLATSDIHLEHKLRKS
HHHHHHHCCCCCCCEEEEHHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHH
RAEVLQIVPEMVAYARSLVDDVEFSCEDAGRSDPEFMYQVIEAAIEAGATTINIPDTVGY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
STPAEFGALIAGIDAHVPNIGQAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECHHC
RAGNASLEELVMALHVRRSYFNGYLGRAEDSSEPLTGIQTEEIYKTSRLVSNLTGMAVQP
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDARTIGLTDNRISLGKLSGRSAVRARLEE
CCEEEECCHHHHHCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
LGYQLDGDDLNDAFARFKELADRKREITDRDLEAIVRQNAQQIEAYYQLAGVQVSCGRDL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCEEEECCCCC
RATATVTLRTSDGEECSQAAIGTGPVDAVCQALNGLVQVPNELVEFSVKSVTEGIDAMGE
EEEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
VTIRLRQDGRLYSGHAADTDVVVAAAQAFVNALNRLVSGQKHSPLHPQRAPLPAPAL
EEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA