Click here to switch to the map view.

The map label for this gene is comEC [H]

Identifier: 148241685

GI number: 148241685

Start: 520246

End: 522099

Strand: Direct

Name: comEC [H]

Synonym: SynRCC307_0586

Alternate gene names: 148241685

Gene position: 520246-522099 (Clockwise)

Preceding gene: 148241684

Following gene: 148241686

Centisome position: 23.38

GC content: 66.67

Gene sequence:

>1854_bases
GTGGCCCGAGCTTGGAGGGCTGGCTTGCTTCTGGTGCCGCTGGCCTTGGGCTGGTGCTGGTGGCGCTGGCCCAGGCCAAC
ACCGGCTGATCCGGTTCATCTGGCGCCTCAATCGGTTGTGTTGGAAGGGCGCATCCAGCGGCCACCCCGCCGTGTGGCTT
CTGGTGCCTGCAGCGCGGTGTTGCAGCTCGAGGGGGCCCATCAAGGGCGCAGCCAGCTGTGGTTTCGCCAGTGCCCCAAG
CAGCTTGATCCCGGTTGGCGTTTGCGCGTGGCTGGCACGCTGCGCCAGCCCAAGCCGCCGCTGCATCCCGTGCTGGGTGA
TCGAGCGGCACAGCTGGCCAGGCAAGGCATTTTCAGCACGCTGGCCGTGCAGCGCTACGAGCTGCTGGAGCGCCAAACGC
AGCCGTTATGGCAGTGGCGGCAGCAACTTGCCGGGCGCTTGCAGGCCTCTGCCGGCCCTCGCCATGGAGCGCTGTTGTCG
GCGCTGGTGATGGGCCGGGCGATGGCGCCTTTGCCTGATGCGATCACCAACTCGTTTCGCGCCGCTGGCCTCAGCCATGC
CTTGGCCGCCAGCGGGTTTCATCTCTCGGTGCTCTTGGGCGTTGTGCTGGCCCTAACGCGGCGAGCCCCGCGCCTGCTTC
GAGTGATCAGCGGGGCTGCGGCATTGGGGCTGTTTGTGCTGTTGGCAGGGCCGATGCCATCGGTGCTCAGGGCGGTGTTG
ATGGGGGCCATAGCGCTATTGATTCGTGAATCCGGTGAACGGGCACGGCCTTTGGGGCTGCTGCTGGTGAGCTTGCTGGC
TCTGCTGTTGTGGCAGCCCGCTTGGCTGTTGGATCTCGGCTTTCAGTTCAGTGCCGTGGCCACCGCTGGCTTGGTGCTCA
CCGCCCCTCGGCTGCAGCAGCAGCTGCCGGCTGCCCTGGCGGTTCCGATCGCCGCATGCCTGTGGACATTGCCGCTGCAG
CTCCTGCATTTCGGCGTGCTGCCGCTCTACGCCGTGCCGGCCAATCTGCTGGCGGCACCACTGCTCACCCTGCTCACCAC
CGGCGCCATGGCGGCGGCTCTGGCGGCGCAACTCGTGCCCCCGCTGCTGCCAGTGATCACTGCTTTGTTGCAGTGGCCTG
CCGAACTGTTGCTGCTGCTGGTGCAGGGCGCGGCGGCTCTGCCTTTGGCCCAGCTGGCCTTGGGCCAACCGCCGCTGGTG
CTGGTCCTGCTGTTGAGCTTGGGGTTAATGCCTTGGCTTTTGGGATGCAGTGGGCCCTGGCGACGCTGGGGCGCAGGGCT
GGTGCTGATCAGTTGCCTCTGGCAAGGGCAGCGTTTGATAGCTGATGAGCTTCTTGAGTTGCCGGTTGGGAAGGGCAACC
TGGTGCTCTTGCGCCATGGCGGCCGCGGCGCCTTGGTGAGCCGCGATGCAGGGCCCCGCAGTTGCAGCCAAGCCCAACGT
TTGCGCCAGGCCCTGGGCTTACCGCGGCTGGATTGGGTGGTCCTCTTGGATCCAGTTCCAGCTGATGATTTCGGCTGCTG
GCAGAAGTTGAGCTCGCAGCTGCAGGTGCTGCCGTGTGGTGAGCTCAGCAGCCCTGGCTTGGTGTTTCGCAGTCAGGTCC
AGGTCCCTGGTGAGGCGCTGCTGCAGCTGGGGCGCCGCTGCTATTGCCTCCGCCAACAGGGCGTGAGCGCCAACATGGGC
GCCCTTGCGGCCTGCGGTCAGTGCCCCACCACGAAGGGAGGCCCACCAAAACCTGCGTCCAGTGCGGCCGCCCGTTTCAG
TGGCGCAAAAAATGGAAGGCCGTGTGGGACGAGGTGCGTTACTGCTCTGAGCGCTGCCGCCGCGAGAGCAAGCGTCGCAG
CCCCTAGGCCCTAA

Upstream 100 bases:

>100_bases
AGTCGTGGCTGGCCGAACGAGAAGCCCTTGGCTTCCCGCTGCTCAAGGGTTAATTCCTGCGGGCCAAGGGGGAACGCTGA
GCTGGCCATGAACGTCGCTG

Downstream 100 bases:

>100_bases
CCTGCGGTTTCAGGGTTTTGCCCTGGTTGGAGAGGTGGCTGAGTGGTCGAAAGCGAACGACTCGAAATCGTTTGAAGGGC
AACCTTCCGTGGGTTCGAAT

Product: membrane metal-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MARAWRAGLLLVPLALGWCWWRWPRPTPADPVHLAPQSVVLEGRIQRPPRRVASGACSAVLQLEGAHQGRSQLWFRQCPK
QLDPGWRLRVAGTLRQPKPPLHPVLGDRAAQLARQGIFSTLAVQRYELLERQTQPLWQWRQQLAGRLQASAGPRHGALLS
ALVMGRAMAPLPDAITNSFRAAGLSHALAASGFHLSVLLGVVLALTRRAPRLLRVISGAAALGLFVLLAGPMPSVLRAVL
MGAIALLIRESGERARPLGLLLVSLLALLLWQPAWLLDLGFQFSAVATAGLVLTAPRLQQQLPAALAVPIAACLWTLPLQ
LLHFGVLPLYAVPANLLAAPLLTLLTTGAMAAALAAQLVPPLLPVITALLQWPAELLLLLVQGAAALPLAQLALGQPPLV
LVLLLSLGLMPWLLGCSGPWRRWGAGLVLISCLWQGQRLIADELLELPVGKGNLVLLRHGGRGALVSRDAGPRSCSQAQR
LRQALGLPRLDWVVLLDPVPADDFGCWQKLSSQLQVLPCGELSSPGLVFRSQVQVPGEALLQLGRRCYCLRQQGVSANMG
ALAACGQCPTTKGGPPKPASSAAARFSGAKNGRPCGTRCVTALSAAAARASVAAPRP

Sequences:

>Translated_617_residues
MARAWRAGLLLVPLALGWCWWRWPRPTPADPVHLAPQSVVLEGRIQRPPRRVASGACSAVLQLEGAHQGRSQLWFRQCPK
QLDPGWRLRVAGTLRQPKPPLHPVLGDRAAQLARQGIFSTLAVQRYELLERQTQPLWQWRQQLAGRLQASAGPRHGALLS
ALVMGRAMAPLPDAITNSFRAAGLSHALAASGFHLSVLLGVVLALTRRAPRLLRVISGAAALGLFVLLAGPMPSVLRAVL
MGAIALLIRESGERARPLGLLLVSLLALLLWQPAWLLDLGFQFSAVATAGLVLTAPRLQQQLPAALAVPIAACLWTLPLQ
LLHFGVLPLYAVPANLLAAPLLTLLTTGAMAAALAAQLVPPLLPVITALLQWPAELLLLLVQGAAALPLAQLALGQPPLV
LVLLLSLGLMPWLLGCSGPWRRWGAGLVLISCLWQGQRLIADELLELPVGKGNLVLLRHGGRGALVSRDAGPRSCSQAQR
LRQALGLPRLDWVVLLDPVPADDFGCWQKLSSQLQVLPCGELSSPGLVFRSQVQVPGEALLQLGRRCYCLRQQGVSANMG
ALAACGQCPTTKGGPPKPASSAAARFSGAKNGRPCGTRCVTALSAAAARASVAAPRP
>Mature_616_residues
ARAWRAGLLLVPLALGWCWWRWPRPTPADPVHLAPQSVVLEGRIQRPPRRVASGACSAVLQLEGAHQGRSQLWFRQCPKQ
LDPGWRLRVAGTLRQPKPPLHPVLGDRAAQLARQGIFSTLAVQRYELLERQTQPLWQWRQQLAGRLQASAGPRHGALLSA
LVMGRAMAPLPDAITNSFRAAGLSHALAASGFHLSVLLGVVLALTRRAPRLLRVISGAAALGLFVLLAGPMPSVLRAVLM
GAIALLIRESGERARPLGLLLVSLLALLLWQPAWLLDLGFQFSAVATAGLVLTAPRLQQQLPAALAVPIAACLWTLPLQL
LHFGVLPLYAVPANLLAAPLLTLLTTGAMAAALAAQLVPPLLPVITALLQWPAELLLLLVQGAAALPLAQLALGQPPLVL
VLLLSLGLMPWLLGCSGPWRRWGAGLVLISCLWQGQRLIADELLELPVGKGNLVLLRHGGRGALVSRDAGPRSCSQAQRL
RQALGLPRLDWVVLLDPVPADDFGCWQKLSSQLQVLPCGELSSPGLVFRSQVQVPGEALLQLGRRCYCLRQQGVSANMGA
LAACGQCPTTKGGPPKPASSAAARFSGAKNGRPCGTRCVTALSAAAARASVAAPRP

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG0658

COG function: function code R; Predicted membrane metal-binding protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=442, Percent_Identity=26.4705882352941, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 65587; Mature: 65456

Theoretical pI: Translated: 11.88; Mature: 11.88

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAWRAGLLLVPLALGWCWWRWPRPTPADPVHLAPQSVVLEGRIQRPPRRVASGACSAV
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHHHHHH
LQLEGAHQGRSQLWFRQCPKQLDPGWRLRVAGTLRQPKPPLHPVLGDRAAQLARQGIFST
HHHCCCCCCHHHHHHHHCHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAVQRYELLERQTQPLWQWRQQLAGRLQASAGPRHGALLSALVMGRAMAPLPDAITNSFR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
AAGLSHALAASGFHLSVLLGVVLALTRRAPRLLRVISGAAALGLFVLLAGPMPSVLRAVL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
MGAIALLIRESGERARPLGLLLVSLLALLLWQPAWLLDLGFQFSAVATAGLVLTAPRLQQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHEECHHHHH
QLPAALAVPIAACLWTLPLQLLHFGVLPLYAVPANLLAAPLLTLLTTGAMAAALAAQLVP
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLLPVITALLQWPAELLLLLVQGAAALPLAQLALGQPPLVLVLLLSLGLMPWLLGCSGPW
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCH
RRWGAGLVLISCLWQGQRLIADELLELPVGKGNLVLLRHGGRGALVSRDAGPRSCSQAQR
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCHHHHHH
LRQALGLPRLDWVVLLDPVPADDFGCWQKLSSQLQVLPCGELSSPGLVFRSQVQVPGEAL
HHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEECCCCCCHHHH
LQLGRRCYCLRQQGVSANMGALAACGQCPTTKGGPPKPASSAAARFSGAKNGRPCGTRCV
HHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHH
TALSAAAARASVAAPRP
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARAWRAGLLLVPLALGWCWWRWPRPTPADPVHLAPQSVVLEGRIQRPPRRVASGACSAV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHHHHHH
LQLEGAHQGRSQLWFRQCPKQLDPGWRLRVAGTLRQPKPPLHPVLGDRAAQLARQGIFST
HHHCCCCCCHHHHHHHHCHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAVQRYELLERQTQPLWQWRQQLAGRLQASAGPRHGALLSALVMGRAMAPLPDAITNSFR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
AAGLSHALAASGFHLSVLLGVVLALTRRAPRLLRVISGAAALGLFVLLAGPMPSVLRAVL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
MGAIALLIRESGERARPLGLLLVSLLALLLWQPAWLLDLGFQFSAVATAGLVLTAPRLQQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHEECHHHHH
QLPAALAVPIAACLWTLPLQLLHFGVLPLYAVPANLLAAPLLTLLTTGAMAAALAAQLVP
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLLPVITALLQWPAELLLLLVQGAAALPLAQLALGQPPLVLVLLLSLGLMPWLLGCSGPW
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCH
RRWGAGLVLISCLWQGQRLIADELLELPVGKGNLVLLRHGGRGALVSRDAGPRSCSQAQR
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCHHHHHH
LRQALGLPRLDWVVLLDPVPADDFGCWQKLSSQLQVLPCGELSSPGLVFRSQVQVPGEAL
HHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEECCCCCCHHHH
LQLGRRCYCLRQQGVSANMGALAACGQCPTTKGGPPKPASSAAARFSGAKNGRPCGTRCV
HHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHH
TALSAAAARASVAAPRP
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]