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The map label for this gene is miaE [H]

Identifier: 148241556

GI number: 148241556

Start: 429860

End: 430531

Strand: Direct

Name: miaE [H]

Synonym: SynRCC307_0457

Alternate gene names: 148241556

Gene position: 429860-430531 (Clockwise)

Preceding gene: 148241555

Following gene: 148241557

Centisome position: 19.32

GC content: 64.29

Gene sequence:

>672_bases
GTGACGGCCACCCCCAGTAAGCCGGTGGCTTCGATTCGTTGGCTCGCGGCCCCCAGCAGCAGCCTTTGGCTGGAGCAGGC
GATCAACCGCCCGGACGAGGTGTTGATCGACCATGCCCATTGCGAGCGCAAAGCCGCTGGGGCGGCCCTGCAGCTGATGT
TTCGCTATCCCTCCAGCGAGACCTTGGCCGAGGCGCTCAGCCCCTTGGCCCAAGAGGAGTTGGAGCATTTCGATCAGGTC
TTGGCGCTACTGCAGCGCCGCGGCATTGCCTTGCGGCCCTTGCCGGCTCCGGCCTATGGCTCGCGGCTGGCCGGAGAGAT
TCGCCGCCAGGAGCCGCTGCGACAACTCGACAGCTTCCTGGTGGCGGGCTTGATTGAAGCCCGCAGCCATGAGCGCATGG
CACTGCTGGCCGAGCACAGCCCCGATCCTGAGTTGCGGGCCCTCTATGGCGATTTGCTCGCCAGCGAAGCGCGCCACTTC
GGCCTCTATTGGCTGCTGTGTGAGCAGCATTGGCCCCGTGAGGTGATCATTCCCCGTCTTGAGCAGCTGGCAGCGGTGGA
AGTTGAGGCCCTCAGTGGTGAATTGGCGCGCCCGGAGGATGTGCGCATGCACTCGGTGGGCGTTCAGCTCCCAAGGAAAA
ATCTTGACCCTGTGGCTTTTACGCCCACTTAA

Upstream 100 bases:

>100_bases
ATCTGGCTGACAAGGCAGTGGGTGTGATCTGCGGCTTTGGTCCGATCAGCTACTACCTCGCCCTTGAAGGGATGGTGGCC
CACTTGCGCAGTAGCCAGCT

Downstream 100 bases:

>100_bases
GGCCAAGCCCCTGGCGTTTTTTTGTTGTTAATCACCCTGGCCCAGGGTGAAGGGGCTTCTTGTTTCTCCCCATGAAGCGA
AGCATCTCTCTGGTCGCTGC

Product: tRNA-(MS[2]IO[6]A)-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MTATPSKPVASIRWLAAPSSSLWLEQAINRPDEVLIDHAHCERKAAGAALQLMFRYPSSETLAEALSPLAQEELEHFDQV
LALLQRRGIALRPLPAPAYGSRLAGEIRRQEPLRQLDSFLVAGLIEARSHERMALLAEHSPDPELRALYGDLLASEARHF
GLYWLLCEQHWPREVIIPRLEQLAAVEVEALSGELARPEDVRMHSVGVQLPRKNLDPVAFTPT

Sequences:

>Translated_223_residues
MTATPSKPVASIRWLAAPSSSLWLEQAINRPDEVLIDHAHCERKAAGAALQLMFRYPSSETLAEALSPLAQEELEHFDQV
LALLQRRGIALRPLPAPAYGSRLAGEIRRQEPLRQLDSFLVAGLIEARSHERMALLAEHSPDPELRALYGDLLASEARHF
GLYWLLCEQHWPREVIIPRLEQLAAVEVEALSGELARPEDVRMHSVGVQLPRKNLDPVAFTPT
>Mature_222_residues
TATPSKPVASIRWLAAPSSSLWLEQAINRPDEVLIDHAHCERKAAGAALQLMFRYPSSETLAEALSPLAQEELEHFDQVL
ALLQRRGIALRPLPAPAYGSRLAGEIRRQEPLRQLDSFLVAGLIEARSHERMALLAEHSPDPELRALYGDLLASEARHFG
LYWLLCEQHWPREVIIPRLEQLAAVEVEALSGELARPEDVRMHSVGVQLPRKNLDPVAFTPT

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 24921; Mature: 24790

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPSKPVASIRWLAAPSSSLWLEQAINRPDEVLIDHAHCERKAAGAALQLMFRYPSSE
CCCCCCCCHHHEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
TLAEALSPLAQEELEHFDQVLALLQRRGIALRPLPAPAYGSRLAGEIRRQEPLRQLDSFL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAGLIEARSHERMALLAEHSPDPELRALYGDLLASEARHFGLYWLLCEQHWPREVIIPRL
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHH
EQLAAVEVEALSGELARPEDVRMHSVGVQLPRKNLDPVAFTPT
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEECCCC
>Mature Secondary Structure 
TATPSKPVASIRWLAAPSSSLWLEQAINRPDEVLIDHAHCERKAAGAALQLMFRYPSSE
CCCCCCCHHHEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
TLAEALSPLAQEELEHFDQVLALLQRRGIALRPLPAPAYGSRLAGEIRRQEPLRQLDSFL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAGLIEARSHERMALLAEHSPDPELRALYGDLLASEARHFGLYWLLCEQHWPREVIIPRL
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHH
EQLAAVEVEALSGELARPEDVRMHSVGVQLPRKNLDPVAFTPT
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]