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The map label for this gene is rpoD [H]

Identifier: 148241530

GI number: 148241530

Start: 409390

End: 410232

Strand: Direct

Name: rpoD [H]

Synonym: SynRCC307_0431

Alternate gene names: 148241530

Gene position: 409390-410232 (Clockwise)

Preceding gene: 148241529

Following gene: 148241531

Centisome position: 18.4

GC content: 63.35

Gene sequence:

>843_bases
ATGGCGGAACCGGTTCGTGCCTATCTCGAGCAGATTGGTCGTTATGCCCTGCTGCGGCCTGAGCAGGAGGTGGAGCTGGC
ACGCTTGGTGCAGCGGGCGCAGCAGCTTGAGCAGCAACTCCAACTAAGCCCCACAGAGCAACGGGAACTGCGGCGCGGCC
GCCGGGCTCGAGAACGCATGATCCAAGCCAACCTGCGCTTGGTGGTGACCGTTGCCAAGACCTACCAAGGGCGAGGTTTG
GATTTGATGGACTTGGTGCAAGAAGGCTCGCTCGGATTGATCCGAGCGGTGGAGAAGTTCGACCCCACCCTGGGTTACCG
CTTCAGTACGTACGCCTGGAATTGGATCCGGCAGGCCATCAGCCGGGGCTTGGAAACCAAAGGCCGCACGCTGCGTTTGC
CCATCCATGTCACCCAGCAGCTCAGGGCATTGCGTCGGGCCCATGAACGCCTCTGGCAACAGGGGCTCAGTACCGATCTC
GTTGCGGTGGCAGCGGAAGCTGGCGTTGATCTCAAGGCCGCCAGCCTGGCGTTGCGGATGGCGCCGGGAACCCTGTCGCT
CGACACGCCGGCGGCCGGTAGTGAGAGCGATCTGCTGCAGCTGTTGCCATCAGCGGAGCCTTCGCCTTTGGATGAGGCTG
AGCGCCACGACGGTTGGGAGCGTATGGGCAGGCAACTTCGCCATTTGCCCGAACGTGATCAACAGGTGTTGCAGTGGCGC
TACCTCACGCCCGAGCGCCAGAAGCTGAGTGTTGTCGGTGAGCAGTTGGGCTGCAGCCGCGAGCGGGCTAGGCAGATTGA
ACAGCGCGCCTTGCTGCGGCTGCGGCTGCGCATGGCGAGTTGA

Upstream 100 bases:

>100_bases
CAAAGCCATCACAAAGGGGAAGACTCCCTGAACCCTTGGCCTAGAGCCCTTGCTGCTCTGCGCTGCCGCCGGTATCGATG
AGGCGCGCAGACCTGCTGCT

Downstream 100 bases:

>100_bases
TCGTGGTGAACATCACATTCCAAGAAACTTTGATACGACAGAGCTAGGTGAATTAAGTCTTTGTTGGTTCGCACAACTCA
CGGCAGCATCAGTGTGGTTG

Product: RNA polymerase sigma factor, sigma-70 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MAEPVRAYLEQIGRYALLRPEQEVELARLVQRAQQLEQQLQLSPTEQRELRRGRRARERMIQANLRLVVTVAKTYQGRGL
DLMDLVQEGSLGLIRAVEKFDPTLGYRFSTYAWNWIRQAISRGLETKGRTLRLPIHVTQQLRALRRAHERLWQQGLSTDL
VAVAAEAGVDLKAASLALRMAPGTLSLDTPAAGSESDLLQLLPSAEPSPLDEAERHDGWERMGRQLRHLPERDQQVLQWR
YLTPERQKLSVVGEQLGCSRERARQIEQRALLRLRLRMAS

Sequences:

>Translated_280_residues
MAEPVRAYLEQIGRYALLRPEQEVELARLVQRAQQLEQQLQLSPTEQRELRRGRRARERMIQANLRLVVTVAKTYQGRGL
DLMDLVQEGSLGLIRAVEKFDPTLGYRFSTYAWNWIRQAISRGLETKGRTLRLPIHVTQQLRALRRAHERLWQQGLSTDL
VAVAAEAGVDLKAASLALRMAPGTLSLDTPAAGSESDLLQLLPSAEPSPLDEAERHDGWERMGRQLRHLPERDQQVLQWR
YLTPERQKLSVVGEQLGCSRERARQIEQRALLRLRLRMAS
>Mature_279_residues
AEPVRAYLEQIGRYALLRPEQEVELARLVQRAQQLEQQLQLSPTEQRELRRGRRARERMIQANLRLVVTVAKTYQGRGLD
LMDLVQEGSLGLIRAVEKFDPTLGYRFSTYAWNWIRQAISRGLETKGRTLRLPIHVTQQLRALRRAHERLWQQGLSTDLV
AVAAEAGVDLKAASLALRMAPGTLSLDTPAAGSESDLLQLLPSAEPSPLDEAERHDGWERMGRQLRHLPERDQQVLQWRY
LTPERQKLSVVGEQLGCSRERARQIEQRALLRLRLRMAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=273, Percent_Identity=40.2930402930403, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1789448, Length=264, Percent_Identity=34.0909090909091, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1789871, Length=235, Percent_Identity=29.3617021276596, Blast_Score=88, Evalue=5e-19,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32133; Mature: 32001

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPVRAYLEQIGRYALLRPEQEVELARLVQRAQQLEQQLQLSPTEQRELRRGRRARERM
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
IQANLRLVVTVAKTYQGRGLDLMDLVQEGSLGLIRAVEKFDPTLGYRFSTYAWNWIRQAI
HHHCHHHEEEEEHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SRGLETKGRTLRLPIHVTQQLRALRRAHERLWQQGLSTDLVAVAAEAGVDLKAASLALRM
HHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHEE
APGTLSLDTPAAGSESDLLQLLPSAEPSPLDEAERHDGWERMGRQLRHLPERDQQVLQWR
CCCCEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHH
YLTPERQKLSVVGEQLGCSRERARQIEQRALLRLRLRMAS
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEPVRAYLEQIGRYALLRPEQEVELARLVQRAQQLEQQLQLSPTEQRELRRGRRARERM
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
IQANLRLVVTVAKTYQGRGLDLMDLVQEGSLGLIRAVEKFDPTLGYRFSTYAWNWIRQAI
HHHCHHHEEEEEHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SRGLETKGRTLRLPIHVTQQLRALRRAHERLWQQGLSTDLVAVAAEAGVDLKAASLALRM
HHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHEE
APGTLSLDTPAAGSESDLLQLLPSAEPSPLDEAERHDGWERMGRQLRHLPERDQQVLQWR
CCCCEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHH
YLTPERQKLSVVGEQLGCSRERARQIEQRALLRLRLRMAS
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA