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The map label for this gene is gmd [H]

Identifier: 148241316

GI number: 148241316

Start: 215923

End: 217029

Strand: Direct

Name: gmd [H]

Synonym: SynRCC307_0217

Alternate gene names: 148241316

Gene position: 215923-217029 (Clockwise)

Preceding gene: 148241314

Following gene: 148241317

Centisome position: 9.7

GC content: 55.92

Gene sequence:

>1107_bases
ATGAGTTCTGATCAGTCCCGCAAGACCGCCCTGATTACGGGGATCACTGGCCAAGACGGCAGCTATTTGGCGGAGCTGCT
GTTGGAGAAGGGCTATGAGGTGCATGGCATCAAGCGACGGGCTAGCAGCTTCAACACCGACCGCATTGATCACCTCTATC
AGGATCCCCATGAGAATGATCCGCGTTTGGTGCTGCATTACGGCGATCTCACCGACAGCACGAATCTGATTCGGATTGTC
CAGCAGGTGCAGCCCGATGAGATTTACAACCTGGGCGCTCAAAGTCACGTGGCCGTGAGCTTTGAAAGCCCCGAATACAC
CGCCAATAGCGATGCACTGGGAACCCTTCGGATCTTGGAAGCTGTGCGGATCCTGGGGCTAACAAACAAGACGCGCATCT
ATCAGGCCAGCACCAGTGAGTTGTATGGCTTGGTGCAGGAGATCCCGCAGAAAGAGAGCACACCATTCTATCCCCGCAGT
CCTTATGGCGTCGCCAAGCTCTACGCCTACTGGATCACGGTCAATTACCGCGAGAGCTATGGCATGTATGCCTGCAATGG
GGTGTTGTTCAACCATGAAAGCCCCAGGCGTGGTGAAACGTTCGTGACCCGCAAGATCACGCGTGGACTTGCCCGGATTG
ATGCGGGATTGGACGACTGCTTGTACATGGGCAATCTCGATTCGCTGCGCGATTGGGGCCATGCCCGCGACTATGTGGAG
ATGCAATGGCGCATGCTTCAGCAGGACAAGCCTGAGGACTTTGTGATTGCCACAGGCCGCCAGGAAAGTGTTCGCTGCTT
TATTGAGCTCACCGCTTTGGAGTTGGGCTGGGGAGCCATGCAGTGGGAAGGCAAAGGGATTGACGAGGTTGGTTGCCGCG
CGGATACCGGAGATGTGGTGGTGAAAATTGATCCGCGCTATTTCCGTCCTGCCGAAGTGGAAACCCTGCTCGGTGACCCC
ACCAAAGCCAAAGAGAAATTGGGGTGGACGCCAACCACCACCTTGGAAGAACTGGTGGCTGAGATGGTGGCAACAGACAA
AGAAGAAGCTCAGAAAGAGGCTTACCTCAAGCGCAAGGGCTTCAACGTCGTTGGAGCGCGCGAGTGA

Upstream 100 bases:

>100_bases
GCACTGTAAGGAGCCTGTCTGTGGCTGGCTTGGAGCCTCTGGCAAGCCGTGACAAACTCCTGGACTTACCAGCTCTTCAA
CCGAGCTCTCGCCGTAGCCC

Downstream 100 bases:

>100_bases
CGTTGATTCAACCCACAGATCGCTTTGCGATTTTTGGTGCCCGCGGAATGGCCGGTAGTGCCATCAGCAGAGCTTTAGGG
CGGCAGGGTTACCAAGACCA

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSSDQSRKTALITGITGQDGSYLAELLLEKGYEVHGIKRRASSFNTDRIDHLYQDPHENDPRLVLHYGDLTDSTNLIRIV
QQVQPDEIYNLGAQSHVAVSFESPEYTANSDALGTLRILEAVRILGLTNKTRIYQASTSELYGLVQEIPQKESTPFYPRS
PYGVAKLYAYWITVNYRESYGMYACNGVLFNHESPRRGETFVTRKITRGLARIDAGLDDCLYMGNLDSLRDWGHARDYVE
MQWRMLQQDKPEDFVIATGRQESVRCFIELTALELGWGAMQWEGKGIDEVGCRADTGDVVVKIDPRYFRPAEVETLLGDP
TKAKEKLGWTPTTTLEELVAEMVATDKEEAQKEAYLKRKGFNVVGARE

Sequences:

>Translated_368_residues
MSSDQSRKTALITGITGQDGSYLAELLLEKGYEVHGIKRRASSFNTDRIDHLYQDPHENDPRLVLHYGDLTDSTNLIRIV
QQVQPDEIYNLGAQSHVAVSFESPEYTANSDALGTLRILEAVRILGLTNKTRIYQASTSELYGLVQEIPQKESTPFYPRS
PYGVAKLYAYWITVNYRESYGMYACNGVLFNHESPRRGETFVTRKITRGLARIDAGLDDCLYMGNLDSLRDWGHARDYVE
MQWRMLQQDKPEDFVIATGRQESVRCFIELTALELGWGAMQWEGKGIDEVGCRADTGDVVVKIDPRYFRPAEVETLLGDP
TKAKEKLGWTPTTTLEELVAEMVATDKEEAQKEAYLKRKGFNVVGARE
>Mature_367_residues
SSDQSRKTALITGITGQDGSYLAELLLEKGYEVHGIKRRASSFNTDRIDHLYQDPHENDPRLVLHYGDLTDSTNLIRIVQ
QVQPDEIYNLGAQSHVAVSFESPEYTANSDALGTLRILEAVRILGLTNKTRIYQASTSELYGLVQEIPQKESTPFYPRSP
YGVAKLYAYWITVNYRESYGMYACNGVLFNHESPRRGETFVTRKITRGLARIDAGLDDCLYMGNLDSLRDWGHARDYVEM
QWRMLQQDKPEDFVIATGRQESVRCFIELTALELGWGAMQWEGKGIDEVGCRADTGDVVVKIDPRYFRPAEVETLLGDPT
KAKEKLGWTPTTTLEELVAEMVATDKEEAQKEAYLKRKGFNVVGARE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=345, Percent_Identity=63.4782608695652, Blast_Score=458, Evalue=1e-129,
Organism=Escherichia coli, GI1788366, Length=371, Percent_Identity=71.6981132075472, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI1788353, Length=298, Percent_Identity=24.496644295302, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48994969, Length=289, Percent_Identity=24.9134948096886, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=348, Percent_Identity=63.5057471264368, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17539422, Length=348, Percent_Identity=63.5057471264368, Blast_Score=473, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI133901788, Length=348, Percent_Identity=63.5057471264368, Blast_Score=473, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI133901786, Length=348, Percent_Identity=63.5057471264368, Blast_Score=473, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI133901790, Length=348, Percent_Identity=63.5057471264368, Blast_Score=473, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17507723, Length=348, Percent_Identity=64.0804597701149, Blast_Score=469, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24158427, Length=350, Percent_Identity=60.8571428571429, Blast_Score=444, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 41683; Mature: 41552

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDQSRKTALITGITGQDGSYLAELLLEKGYEVHGIKRRASSFNTDRIDHLYQDPHEND
CCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHCCCCCCC
PRLVLHYGDLTDSTNLIRIVQQVQPDEIYNLGAQSHVAVSFESPEYTANSDALGTLRILE
CEEEEEECCCCCCHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
AVRILGLTNKTRIYQASTSELYGLVQEIPQKESTPFYPRSPYGVAKLYAYWITVNYRESY
HHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEEEEEHHCC
GMYACNGVLFNHESPRRGETFVTRKITRGLARIDAGLDDCLYMGNLDSLRDWGHARDYVE
CEEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHEECCCCHHHHHHHHHHHHHH
MQWRMLQQDKPEDFVIATGRQESVRCFIELTALELGWGAMQWEGKGIDEVGCRADTGDVV
HHHHHHHCCCCCCEEEECCCCCCEEEEEEEEEEEECCCEEEECCCCCHHCCCCCCCCCEE
VKIDPRYFRPAEVETLLGDPTKAKEKLGWTPTTTLEELVAEMVATDKEEAQKEAYLKRKG
EEECCCCCCCCHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCC
FNVVGARE
CCCCCCCC
>Mature Secondary Structure 
SSDQSRKTALITGITGQDGSYLAELLLEKGYEVHGIKRRASSFNTDRIDHLYQDPHEND
CCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHCCCCCCC
PRLVLHYGDLTDSTNLIRIVQQVQPDEIYNLGAQSHVAVSFESPEYTANSDALGTLRILE
CEEEEEECCCCCCHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
AVRILGLTNKTRIYQASTSELYGLVQEIPQKESTPFYPRSPYGVAKLYAYWITVNYRESY
HHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEEEEEHHCC
GMYACNGVLFNHESPRRGETFVTRKITRGLARIDAGLDDCLYMGNLDSLRDWGHARDYVE
CEEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHEECCCCHHHHHHHHHHHHHH
MQWRMLQQDKPEDFVIATGRQESVRCFIELTALELGWGAMQWEGKGIDEVGCRADTGDVV
HHHHHHHCCCCCCEEEECCCCCCEEEEEEEEEEEECCCEEEECCCCCHHCCCCCCCCCEE
VKIDPRYFRPAEVETLLGDPTKAKEKLGWTPTTTLEELVAEMVATDKEEAQKEAYLKRKG
EEECCCCCCCCHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCC
FNVVGARE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]