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The map label for this gene is ndhK

Identifier: 148241313

GI number: 148241313

Start: 213170

End: 213898

Strand: Direct

Name: ndhK

Synonym: SynRCC307_0214

Alternate gene names: 148241313

Gene position: 213170-213898 (Clockwise)

Preceding gene: 148241312

Following gene: 148241314

Centisome position: 9.58

GC content: 57.75

Gene sequence:

>729_bases
ATGTCAGACACCTCACTTGATTCGATGCGCGATTTGCGCGCAGCCAGTTGCGGGCCTGTTGGCTCACCAGAGGTCACCAC
TGATCTCTCTGAGAACGTCATCCTCACCAGCCTGGATGACCTGCACAACTGGGCTCGCCTGAGCAGCCTTTGGCCCCTGC
TCTACGGCACGGCTTGCTGCTTCATTGAATTTGCTGCGCTGATTGGCTCGCGGTTTGATTTCGATCGTTTTGGCTTGGTC
CCCAGATCCAGCCCGCGGCAAGCCGATCTTCTGATCGTGGCTGGCACGGTCACCATGAAGATGGCTCCTGCTCTGGTCAG
GCTGTATGAGCAGATGCCTGAGCCGAAATACGTCATCGCCATGGGGGCCTGCACCATCACTGGTGGCATGTTCAGCGCCG
ATTCCACGACGGCCGTTCGTGGTGTCGACAAACTCATCCCAGTTGATCTGTATTTGCCTGGCTGCCCGCCGCGTCCAGAG
GCCATCTTTGATGCAGTGATCAAGTTGCGCAAAAAGGTTGGTGATGAGGCCATCGCCGAGCGGGGCAAACTTGAGCAAAC
CCACCGCTACAGCACGGTTGAACACCGCATGACGGCCGTTGATCCGATTGTGGATGGCCGTTATCTGCGCGCTGAAACCC
AACAAAAGGCACTGGCTGCAGCGGAAGCCGTCTCTCCTCTCAACTTGGCTCCAGCGGAGCAACCCGAAACAATCCCCGCC
AAATCATGA

Upstream 100 bases:

>100_bases
CCTGTTGGCGTTCATCGAAGCCTTGGTGTTTATCGCCATCTTGGTGGTGGCGCTTGCCTATGCCTGGCGCAAAGGCGCCC
TGGAGTGGAGCTGAACTGTT

Downstream 100 bases:

>100_bases
GCGAGACCCCCACCTCACCCAACCAGGATTTGCCGGAAGCTCCCCAGGCTGGTCCTCTCTCCCAATGGCTTACGAGCCAA
GGTTTTGATCACCAAATTCT

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSDTSLDSMRDLRAASCGPVGSPEVTTDLSENVILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGLV
PRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPE
AIFDAVIKLRKKVGDEAIAERGKLEQTHRYSTVEHRMTAVDPIVDGRYLRAETQQKALAAAEAVSPLNLAPAEQPETIPA
KS

Sequences:

>Translated_242_residues
MSDTSLDSMRDLRAASCGPVGSPEVTTDLSENVILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGLV
PRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPE
AIFDAVIKLRKKVGDEAIAERGKLEQTHRYSTVEHRMTAVDPIVDGRYLRAETQQKALAAAEAVSPLNLAPAEQPETIPA
KS
>Mature_241_residues
SDTSLDSMRDLRAASCGPVGSPEVTTDLSENVILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGLVP
RSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEA
IFDAVIKLRKKVGDEAIAERGKLEQTHRYSTVEHRMTAVDPIVDGRYLRAETQQKALAAAEAVSPLNLAPAEQPETIPAK
S

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=52.4475524475524, Blast_Score=176, Evalue=1e-44,
Organism=Escherichia coli, GI1788624, Length=164, Percent_Identity=48.780487804878, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1789074, Length=106, Percent_Identity=43.3962264150943, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788834, Length=106, Percent_Identity=42.4528301886792, Blast_Score=91, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=153, Percent_Identity=49.6732026143791, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=55.3956834532374, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=55.3956834532374, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24651058, Length=136, Percent_Identity=55.1470588235294, Blast_Score=170, Evalue=7e-43,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NDHK_SYNR3 (A5GQF8)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001226470.1
- ProteinModelPortal:   A5GQF8
- SMR:   A5GQF8
- STRING:   A5GQF8
- GeneID:   5156588
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_0214
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   ITMKMAP
- ProtClustDB:   PRK06411
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.6.5.3

Molecular weight: Translated: 26297; Mature: 26165

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTSLDSMRDLRAASCGPVGSPEVTTDLSENVILTSLDDLHNWARLSSLWPLLYGTACC
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
FIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIA
HHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEE
MGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGDEAIAE
ECCCEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHH
RGKLEQTHRYSTVEHRMTAVDPIVDGRYLRAETQQKALAAAEAVSPLNLAPAEQPETIPA
CCCHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
KS
CC
>Mature Secondary Structure 
SDTSLDSMRDLRAASCGPVGSPEVTTDLSENVILTSLDDLHNWARLSSLWPLLYGTACC
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
FIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIA
HHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEE
MGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGDEAIAE
ECCCEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHH
RGKLEQTHRYSTVEHRMTAVDPIVDGRYLRAETQQKALAAAEAVSPLNLAPAEQPETIPA
CCCHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA