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The map label for this gene is galE [C]

Identifier: 148241303

GI number: 148241303

Start: 207383

End: 208579

Strand: Direct

Name: galE [C]

Synonym: SynRCC307_0204

Alternate gene names: 148241303

Gene position: 207383-208579 (Clockwise)

Preceding gene: 148241302

Following gene: 148241304

Centisome position: 9.32

GC content: 60.23

Gene sequence:

>1197_bases
ATGAAGGTCCTGGTCCTTGGTGGTGACGGCTTTTGCGGCTGGCCATGCGCCGTGAATTTGGCCGATCAGGGCCACGACGT
TCTGATCGTTGACAACCTCAGCCGCCGCAAGATCGACATCGATCTGGAGGTGGAATCCCTAACCCCAATCACCAACATTG
GTGAGCGCCTCAAGGCCTGGAGTGAGATCGGCGGCAAACCGATGCGCTTTGTCCACCTGGACATCGCCCATGAGTACCAG
CGGCTCCTGGATCTGCTTCTCGAGGAGAAGCCCGACTCGGTGGTGCATTTCGCCGAGCAACGCGCTGCCCCCTATTCGAT
GAAGAGCAGCGCCACCAAGCGCTACACCGTCGATAACAACGTCAACGGCACCCACAACCTGCTGGCCGCCATCGTTGAAA
GTGGCCTCGATATTCACGTGGTGCACCTCGGCACCATGGGGGTCTACGGCTATGGCTCCCACCGCGGCGCCACCATCCCC
GAGGGCTACCTGAAGGTGGAAGTGCCACAGCCCGATGGCAGCCGTTTTGAGGAGGAGATCCTCCACCCCGCCAGCCCGGG
CAGCGTCTATCACATGACCAAGACGCTGGATCAGCTGCTCTTCCTCTACTACAACAAAAACGATCAGGTCCGCATCACCG
ATCTGCACCAGGGCATCGTTTGGGGCACCAACACTGAAGCCACCGACCGCGATCCACGCTTGACCAATCGCTTTGATTAC
GACGGCGACTACGGCACGGTCTTGAACCGCTTCTTGATGCAGGCGGCCATTGGCTACCCCCTGACCGTGCATGGCACCGG
CGGCCAGACCCGCGCCTTCATCCACATCCGCGACTCGGTGCGCTGCGTGCAGCTGGCCTTGGACAACCCTCCGGTCAAAG
GCGAGCGGGTCAAGATCTTCAACCAGATGACCGAAAGCCATCAGGTGGGAGAACTGGCCAAGAAGGTGGCCGCACTTACC
GGCGCGCAGGTCAACAACCTGCCCAATCCCCGCAATGAAGCGGTGGAAAACGATCTGATCGTGGACAACCGCTGCTTCAT
TGAGCTGGGCCTCAATCCCACAACCCTCGATGATGGGTTGCTGAAGGAAGTGGTGGAGATCGCCACCCGTTATGCCGACC
GCTGCGACCGCAACCGGATCCTCTGCACGTCTGCCTGGACCAAAACCCAAGAACAGGCCATTCGTGCAGGCTCATGA

Upstream 100 bases:

>100_bases
CAACCTACTGATTTGTCTTTAAAGTCCCTTGACCTGTTAAGGCTTTTTTACCGTTCTGATTACGAACTTATGGATATAAT
CGGTTTTTAGCCAAATTTTG

Downstream 100 bases:

>100_bases
CCTTGACTTCTTAAATCTCTGATAATTCCCCCTTCGTTTTGTGACCAGTTCTAATCAACCTCTGGTAAGCATAATTATTC
CCTGCTTTAATTGTGCTTCC

Product: sulfolipid biosynthesis protein (UDP-sulfoquinovose synthase)

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Nucleoside-Diphosphate-Sugar Epimerase; Epimerase; Nucleoside-Diphosphate-Sugar Epimerases; UDP Sulfoquinovose Synthase; DTDP-Glucose 4-6-Dehydratase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKVLVLGGDGFCGWPCAVNLADQGHDVLIVDNLSRRKIDIDLEVESLTPITNIGERLKAWSEIGGKPMRFVHLDIAHEYQ
RLLDLLLEEKPDSVVHFAEQRAAPYSMKSSATKRYTVDNNVNGTHNLLAAIVESGLDIHVVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQVRITDLHQGIVWGTNTEATDRDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHIRDSVRCVQLALDNPPVKGERVKIFNQMTESHQVGELAKKVAALT
GAQVNNLPNPRNEAVENDLIVDNRCFIELGLNPTTLDDGLLKEVVEIATRYADRCDRNRILCTSAWTKTQEQAIRAGS

Sequences:

>Translated_398_residues
MKVLVLGGDGFCGWPCAVNLADQGHDVLIVDNLSRRKIDIDLEVESLTPITNIGERLKAWSEIGGKPMRFVHLDIAHEYQ
RLLDLLLEEKPDSVVHFAEQRAAPYSMKSSATKRYTVDNNVNGTHNLLAAIVESGLDIHVVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQVRITDLHQGIVWGTNTEATDRDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHIRDSVRCVQLALDNPPVKGERVKIFNQMTESHQVGELAKKVAALT
GAQVNNLPNPRNEAVENDLIVDNRCFIELGLNPTTLDDGLLKEVVEIATRYADRCDRNRILCTSAWTKTQEQAIRAGS
>Mature_398_residues
MKVLVLGGDGFCGWPCAVNLADQGHDVLIVDNLSRRKIDIDLEVESLTPITNIGERLKAWSEIGGKPMRFVHLDIAHEYQ
RLLDLLLEEKPDSVVHFAEQRAAPYSMKSSATKRYTVDNNVNGTHNLLAAIVESGLDIHVVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQVRITDLHQGIVWGTNTEATDRDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHIRDSVRCVQLALDNPPVKGERVKIFNQMTESHQVGELAKKVAALT
GAQVNNLPNPRNEAVENDLIVDNRCFIELGLNPTTLDDGLLKEVVEIATRYADRCDRNRILCTSAWTKTQEQAIRAGS

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786974, Length=131, Percent_Identity=37.4045801526718, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 44341; Mature: 44341

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVLGGDGFCGWPCAVNLADQGHDVLIVDNLSRRKIDIDLEVESLTPITNIGERLKAW
CEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
SEIGGKPMRFVHLDIAHEYQRLLDLLLEEKPDSVVHFAEQRAAPYSMKSSATKRYTVDNN
HHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHCCCCEEEEECCC
VNGTHNLLAAIVESGLDIHVVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCHHHHHHHHHHHCCCEEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDQVRITDLHQGIVWGTNTEATDRDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHIRDSVRCVQLALDNPPVKGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHH
NQMTESHQVGELAKKVAALTGAQVNNLPNPRNEAVENDLIVDNRCFIELGLNPTTLDDGL
HHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHCCCEEECCEEEEEECCCCCCCCHHH
LKEVVEIATRYADRCDRNRILCTSAWTKTQEQAIRAGS
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCC
>Mature Secondary Structure
MKVLVLGGDGFCGWPCAVNLADQGHDVLIVDNLSRRKIDIDLEVESLTPITNIGERLKAW
CEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
SEIGGKPMRFVHLDIAHEYQRLLDLLLEEKPDSVVHFAEQRAAPYSMKSSATKRYTVDNN
HHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHCCCCEEEEECCC
VNGTHNLLAAIVESGLDIHVVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCHHHHHHHHHHHCCCEEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDQVRITDLHQGIVWGTNTEATDRDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHIRDSVRCVQLALDNPPVKGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHH
NQMTESHQVGELAKKVAALTGAQVNNLPNPRNEAVENDLIVDNRCFIELGLNPTTLDDGL
HHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHCCCEEECCEEEEEECCCCCCCCHHH
LKEVVEIATRYADRCDRNRILCTSAWTKTQEQAIRAGS
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA