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The map label for this gene is rhtC [H]

Identifier: 147675763

GI number: 147675763

Start: 2723532

End: 2724200

Strand: Reverse

Name: rhtC [H]

Synonym: VC0395_A2572

Alternate gene names: 147675763

Gene position: 2724200-2723532 (Counterclockwise)

Preceding gene: 147673771

Following gene: 147674499

Centisome position: 90.08

GC content: 51.42

Gene sequence:

>669_bases
ATGAACGAAATGTCCATTTTAGCCACCTTAGCCGGTGTGCATTTTATTGCCTTATTAAGCCCGGGGCCTGATGTCGCCTT
AGTGGTGCAAAACGCTACACAGCACGGGCGTAAAACTGGCGTGATGATCGCGTTAGGTTTGTCGTGCGGCATTTTAGTCC
ATTTGATTTTGAGCCTGTCCGGCATTAGCTACTTAGTCAAACAGCAGCCAATGCTGTTTAACCTGTTACAACTGGCCGGC
GGCAGCTACTTGCTTTATTTAGGGGCCGGCGCTTTGCAATCAGTGATGGCGCAAAAAAACGCTAGCACACCGACGCACAG
CCCTGCGCCCTCCATTCTCGGCAACCGCCGTCAAGCCTTCACAAAAGGCATGATGACCAATCTCTTGAATCCCAAAGCTC
TCGTGTTTTTTGTCAGCCTGTTATCTAGCCTGATCCCAGCCAGCATGTCTGTGTCTGGCAAGGTGAGTGCCGCTGCGATT
TTGGTTGGCTTATCCCTAACTTGGTTCTCCTGTTTAGCTTGGCTGCTTACCACGTCTGCCATGCAACAACGCATGCAGCG
CATTACGCGATCCGTCGATAGCATATGCGCTGCGGTGTTTATCTTGGCCGGTGGCGTGATTTTATGGCAAGCAAGCCGCG
CTATAGCACAAACCTTCGGATGGCTATGA

Upstream 100 bases:

>100_bases
CTTCTTTGATCACTCGCTTTTCTTAACCACGCTGTCAAAAATTTACAAGCTCCTCGCGCTCGCCGCGCGGCATACTGGGT
GAATAATGTGAGGAGAAAGC

Downstream 100 bases:

>100_bases
TCCGACTTTGGCCGATCATGCTGTGCGAGATCTCTTACAAAGCGGTAGGACAAATTCGCTTTCTGCGATAAAAAACACTG
ACAAAAACAACATAAGCAAT

Product: hypothetical protein

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNEMSILATLAGVHFIALLSPGPDVALVVQNATQHGRKTGVMIALGLSCGILVHLILSLSGISYLVKQQPMLFNLLQLAG
GSYLLYLGAGALQSVMAQKNASTPTHSPAPSILGNRRQAFTKGMMTNLLNPKALVFFVSLLSSLIPASMSVSGKVSAAAI
LVGLSLTWFSCLAWLLTTSAMQQRMQRITRSVDSICAAVFILAGGVILWQASRAIAQTFGWL

Sequences:

>Translated_222_residues
MNEMSILATLAGVHFIALLSPGPDVALVVQNATQHGRKTGVMIALGLSCGILVHLILSLSGISYLVKQQPMLFNLLQLAG
GSYLLYLGAGALQSVMAQKNASTPTHSPAPSILGNRRQAFTKGMMTNLLNPKALVFFVSLLSSLIPASMSVSGKVSAAAI
LVGLSLTWFSCLAWLLTTSAMQQRMQRITRSVDSICAAVFILAGGVILWQASRAIAQTFGWL
>Mature_222_residues
MNEMSILATLAGVHFIALLSPGPDVALVVQNATQHGRKTGVMIALGLSCGILVHLILSLSGISYLVKQQPMLFNLLQLAG
GSYLLYLGAGALQSVMAQKNASTPTHSPAPSILGNRRQAFTKGMMTNLLNPKALVFFVSLLSSLIPASMSVSGKVSAAAI
LVGLSLTWFSCLAWLLTTSAMQQRMQRITRSVDSICAAVFILAGGVILWQASRAIAQTFGWL

Specific function: Conducts the efflux of threonine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994978, Length=193, Percent_Identity=32.1243523316062, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788099, Length=186, Percent_Identity=29.5698924731183, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1786522, Length=207, Percent_Identity=27.536231884058, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23485; Mature: 23485

Theoretical pI: Translated: 10.88; Mature: 10.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEMSILATLAGVHFIALLSPGPDVALVVQNATQHGRKTGVMIALGLSCGILVHLILSLS
CCHHHHHHHHHHHHHHHEECCCCCEEEEEECCHHCCCCCCCEEEEHHHHHHHHHHHHHHH
GISYLVKQQPMLFNLLQLAGGSYLLYLGAGALQSVMAQKNASTPTHSPAPSILGNRRQAF
HHHHHHHCCCHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHH
TKGMMTNLLNPKALVFFVSLLSSLIPASMSVSGKVSAAAILVGLSLTWFSCLAWLLTTSA
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MQQRMQRITRSVDSICAAVFILAGGVILWQASRAIAQTFGWL
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEMSILATLAGVHFIALLSPGPDVALVVQNATQHGRKTGVMIALGLSCGILVHLILSLS
CCHHHHHHHHHHHHHHHEECCCCCEEEEEECCHHCCCCCCCEEEEHHHHHHHHHHHHHHH
GISYLVKQQPMLFNLLQLAGGSYLLYLGAGALQSVMAQKNASTPTHSPAPSILGNRRQAF
HHHHHHHCCCHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHH
TKGMMTNLLNPKALVFFVSLLSSLIPASMSVSGKVSAAAILVGLSLTWFSCLAWLLTTSA
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MQQRMQRITRSVDSICAAVFILAGGVILWQASRAIAQTFGWL
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]