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The map label for this gene is mscS [H]

Identifier: 147675699

GI number: 147675699

Start: 1438783

End: 1439661

Strand: Reverse

Name: mscS [H]

Synonym: VC0395_A1350

Alternate gene names: 147675699

Gene position: 1439661-1438783 (Counterclockwise)

Preceding gene: 147675032

Following gene: 147675365

Centisome position: 47.61

GC content: 45.39

Gene sequence:

>879_bases
GTGGTATTTTCAATTGTTCGTCACTGGTCAAATTCGCTGGTCAACTGGCTGGCAAGTAATGCGGTTCAACACCTATTCCA
ACTCTTTATCTTTTTATTGATTCTACTGGCGGCGAAGGGCGGAGCGAAAGTGACTCGCACTGTAGTACGTTCTGCTGTGA
ACTCGAAAAACATCCGTATCTCTCATTTGATGCAGGATTTCTTTGTTTCTATGTCCGGCAAAGTAGTGTGGGTTATCGGG
ATTTTGGTCGGCTTATCGCAGATTGGTCTCAATCTCGCGCCAGTATTAACCGGTTTTGGTATTGCAGGGGTGATCATTGG
TTTCGCGTTGCAAGATACTTTGTCTAACTTCGCAGCAGGCATGATGTTACTGATTTACCGACCATTTGATGTGGGGGATT
TTGTGCATGCGGGAGGTGTGGATGGCAAAGTGAGCCACATGAGTTTGGTCAATACCACGATTCGTACGTTCGATAACCAG
ATCATTATTGTGCCCAATAGCAAAATTTGGGGGGATGTGATCAAAAACGTTACCCATGAACGGATCCGACGAGTCGATAT
GGTGTTTGGCATTGGGTATAGCGATGATTTGCTTAAAGCAGAAGCAGTACTGACCGATATCGTAAAGTCGCATCCCGCAG
TGCTAAAAACACCTGAGCCGAATATCAAAGTGCATACGCTTAACACCTCATCGATTGACTTTATCGTGCGTCCTTGGGTG
AAAACCGACGATTACTGGGATGTGTATTGGGATGTCACTAAAGAAGTTAAGCTGCGCTTTGACCGGGAAGGTATTTCTAT
TCCTTTCCCACAACAAGATGTGCATTTGCATCTGGTGGATCAAAAAGCCTTTATCCCACCTACACAGCCCACTTCTTAG

Upstream 100 bases:

>100_bases
GGTGGGGATTGCCGATCAATTTGAGATAGAAACCGCCGACTATAAGCGACAGATTTTCCAAGTGACGGGCAGTATTACTC
AGGATTTGTTGAACACTCAG

Downstream 100 bases:

>100_bases
TGTTGGTAAGTGTGACTATTTTGTTTGGCAGAGCGATGTTGTCGCTCTGCCATTAGGTTCAACGGAGGGCGAGATCTTTA
AAGGATGAGCATTGGTATCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MVFSIVRHWSNSLVNWLASNAVQHLFQLFIFLLILLAAKGGAKVTRTVVRSAVNSKNIRISHLMQDFFVSMSGKVVWVIG
ILVGLSQIGLNLAPVLTGFGIAGVIIGFALQDTLSNFAAGMMLLIYRPFDVGDFVHAGGVDGKVSHMSLVNTTIRTFDNQ
IIIVPNSKIWGDVIKNVTHERIRRVDMVFGIGYSDDLLKAEAVLTDIVKSHPAVLKTPEPNIKVHTLNTSSIDFIVRPWV
KTDDYWDVYWDVTKEVKLRFDREGISIPFPQQDVHLHLVDQKAFIPPTQPTS

Sequences:

>Translated_292_residues
MVFSIVRHWSNSLVNWLASNAVQHLFQLFIFLLILLAAKGGAKVTRTVVRSAVNSKNIRISHLMQDFFVSMSGKVVWVIG
ILVGLSQIGLNLAPVLTGFGIAGVIIGFALQDTLSNFAAGMMLLIYRPFDVGDFVHAGGVDGKVSHMSLVNTTIRTFDNQ
IIIVPNSKIWGDVIKNVTHERIRRVDMVFGIGYSDDLLKAEAVLTDIVKSHPAVLKTPEPNIKVHTLNTSSIDFIVRPWV
KTDDYWDVYWDVTKEVKLRFDREGISIPFPQQDVHLHLVDQKAFIPPTQPTS
>Mature_292_residues
MVFSIVRHWSNSLVNWLASNAVQHLFQLFIFLLILLAAKGGAKVTRTVVRSAVNSKNIRISHLMQDFFVSMSGKVVWVIG
ILVGLSQIGLNLAPVLTGFGIAGVIIGFALQDTLSNFAAGMMLLIYRPFDVGDFVHAGGVDGKVSHMSLVNTTIRTFDNQ
IIIVPNSKIWGDVIKNVTHERIRRVDMVFGIGYSDDLLKAEAVLTDIVKSHPAVLKTPEPNIKVHTLNTSSIDFIVRPWV
KTDDYWDVYWDVTKEVKLRFDREGISIPFPQQDVHLHLVDQKAFIPPTQPTS

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=280, Percent_Identity=34.6428571428571, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1787591, Length=286, Percent_Identity=26.2237762237762, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786670, Length=176, Percent_Identity=30.1136363636364, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367355, Length=215, Percent_Identity=23.7209302325581, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 32585; Mature: 32585

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFSIVRHWSNSLVNWLASNAVQHLFQLFIFLLILLAAKGGAKVTRTVVRSAVNSKNIRI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHH
SHLMQDFFVSMSGKVVWVIGILVGLSQIGLNLAPVLTGFGIAGVIIGFALQDTLSNFAAG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
MMLLIYRPFDVGDFVHAGGVDGKVSHMSLVNTTIRTFDNQIIIVPNSKIWGDVIKNVTHE
HHEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHH
RIRRVDMVFGIGYSDDLLKAEAVLTDIVKSHPAVLKTPEPNIKVHTLNTSSIDFIVRPWV
HHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCEEEEECCC
KTDDYWDVYWDVTKEVKLRFDREGISIPFPQQDVHLHLVDQKAFIPPTQPTS
CCCCCEEEEEECCHHEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MVFSIVRHWSNSLVNWLASNAVQHLFQLFIFLLILLAAKGGAKVTRTVVRSAVNSKNIRI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHH
SHLMQDFFVSMSGKVVWVIGILVGLSQIGLNLAPVLTGFGIAGVIIGFALQDTLSNFAAG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
MMLLIYRPFDVGDFVHAGGVDGKVSHMSLVNTTIRTFDNQIIIVPNSKIWGDVIKNVTHE
HHEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHH
RIRRVDMVFGIGYSDDLLKAEAVLTDIVKSHPAVLKTPEPNIKVHTLNTSSIDFIVRPWV
HHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCEEEEECCC
KTDDYWDVYWDVTKEVKLRFDREGISIPFPQQDVHLHLVDQKAFIPPTQPTS
CCCCCEEEEEECCHHEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]