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The map label for this gene is citB [H]

Identifier: 147675694

GI number: 147675694

Start: 336304

End: 336996

Strand: Reverse

Name: citB [H]

Synonym: VC0395_A0317

Alternate gene names: 147675694

Gene position: 336996-336304 (Counterclockwise)

Preceding gene: 147673181

Following gene: 147674088

Centisome position: 11.14

GC content: 48.48

Gene sequence:

>693_bases
ATGATGGAATTAATCGACGTATTGATCGTAGAGGATGAAACGAGTATTGCTGACGTGCACAGCTTTTATCTCAAACAAAC
CGCCCGCTTTCGCCCAGTCGGCGTGGCTCAAAGTATCAACGAAGCGCGCAATATGGTGCGCATTCTCAAACCTAAACTGA
TTTTCTTGGATAACTACCTGCCTGATGGTCGTGGCATTGAGTTTTTGAAAGAGCTAACCCATCAACCACAAGCGCCGGAT
GTCATTTTTATCACCGCCGCTTCGGATATGGAGACCGTGCGCGAAGCCGTACGCTGCGGGGTTTTTGATTACCTGTTAAA
ACCGATCGCCTACGATCGAGTACAAAACTCGCTGGAACGGTATCTGAAGTACATCAGCTCGCTGCGCGCCATGCGCGCCA
ATGACAGTGTGAATCAAAGACACGTCGATGAGCTATTTAATTTTCAAGCCAAAACCGAGCAGCTTGATGGGTTGCCGAAA
GGGATTGATGAGCTGACCTTAATCAAAATCCAAGAGATTTATCACCATGAACCGCAAGCGGCCTACACCGCAGAATTGTT
AGGACAAGCGATTGGGATCAGCAAAACCACGGCCAGACGTTATTTGGAATATTGTGTGGCCAGTGGTTTTCTGGAAGCCT
GTATTCAGCATGGTCGGGTGGGCCGTCCCGAACGACTTTATCAAAAGCGCTAA

Upstream 100 bases:

>100_bases
CAAGGGACTATTTTTTCTATTTTTATTCCCAATAGACCCAAAACGCTTGCGGCGGAAAACCTTCCAGCGCTTGCCGAACG
GGCATAAGAAGAGTTTGATT

Downstream 100 bases:

>100_bases
CCCACTCAGCATGCCGAATGCATCAGGCATGAGTTACAAAGTGTAGTGAAAGGGGATCACTTCGGTGCTCTCCCCTATTT
CCAACATTCCTTCTAAGTTG

Product: transcriptional regulator CitB

Products: NA

Alternate protein names: Destabilizer of plasmid inheritance [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MMELIDVLIVEDETSIADVHSFYLKQTARFRPVGVAQSINEARNMVRILKPKLIFLDNYLPDGRGIEFLKELTHQPQAPD
VIFITAASDMETVREAVRCGVFDYLLKPIAYDRVQNSLERYLKYISSLRAMRANDSVNQRHVDELFNFQAKTEQLDGLPK
GIDELTLIKIQEIYHHEPQAAYTAELLGQAIGISKTTARRYLEYCVASGFLEACIQHGRVGRPERLYQKR

Sequences:

>Translated_230_residues
MMELIDVLIVEDETSIADVHSFYLKQTARFRPVGVAQSINEARNMVRILKPKLIFLDNYLPDGRGIEFLKELTHQPQAPD
VIFITAASDMETVREAVRCGVFDYLLKPIAYDRVQNSLERYLKYISSLRAMRANDSVNQRHVDELFNFQAKTEQLDGLPK
GIDELTLIKIQEIYHHEPQAAYTAELLGQAIGISKTTARRYLEYCVASGFLEACIQHGRVGRPERLYQKR
>Mature_230_residues
MMELIDVLIVEDETSIADVHSFYLKQTARFRPVGVAQSINEARNMVRILKPKLIFLDNYLPDGRGIEFLKELTHQPQAPD
VIFITAASDMETVREAVRCGVFDYLLKPIAYDRVQNSLERYLKYISSLRAMRANDSVNQRHVDELFNFQAKTEQLDGLPK
GIDELTLIKIQEIYHHEPQAAYTAELLGQAIGISKTTARRYLEYCVASGFLEACIQHGRVGRPERLYQKR

Specific function: Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox c

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786838, Length=229, Percent_Identity=49.3449781659389, Blast_Score=223, Evalue=8e-60,
Organism=Escherichia coli, GI1790566, Length=223, Percent_Identity=27.3542600896861, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790437, Length=128, Percent_Identity=34.375, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788550, Length=129, Percent_Identity=31.7829457364341, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786599, Length=124, Percent_Identity=32.258064516129, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 26406; Mature: 26406

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMELIDVLIVEDETSIADVHSFYLKQTARFRPVGVAQSINEARNMVRILKPKLIFLDNYL
CHHHHEEHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHEEEECCC
PDGRGIEFLKELTHQPQAPDVIFITAASDMETVREAVRCGVFDYLLKPIAYDRVQNSLER
CCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLKYISSLRAMRANDSVNQRHVDELFNFQAKTEQLDGLPKGIDELTLIKIQEIYHHEPQA
HHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCH
AYTAELLGQAIGISKTTARRYLEYCVASGFLEACIQHGRVGRPERLYQKR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MMELIDVLIVEDETSIADVHSFYLKQTARFRPVGVAQSINEARNMVRILKPKLIFLDNYL
CHHHHEEHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHEEEECCC
PDGRGIEFLKELTHQPQAPDVIFITAASDMETVREAVRCGVFDYLLKPIAYDRVQNSLER
CCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLKYISSLRAMRANDSVNQRHVDELFNFQAKTEQLDGLPKGIDELTLIKIQEIYHHEPQA
HHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCH
AYTAELLGQAIGISKTTARRYLEYCVASGFLEACIQHGRVGRPERLYQKR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]