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The map label for this gene is ligA

Identifier: 147675693

GI number: 147675693

Start: 525975

End: 527984

Strand: Direct

Name: ligA

Synonym: VC0395_A0492

Alternate gene names: 147675693

Gene position: 525975-527984 (Clockwise)

Preceding gene: 147673252

Following gene: 147674775

Centisome position: 17.39

GC content: 48.06

Gene sequence:

>2010_bases
ATGTCAGATGTCGCGCAACGATTAACAGAATTAAGAAAAACCTTGCATGAGCATGGTGTCCGCTATTACGTAGAAGATGC
CCCCACCATTCCGGATGCAGAATACGATCGCTTAATGCGCGAGCTGCTGGAATTGGAAGCGGCTCACCCGGAATTGATGA
GCTCGGACTCGCCCAGTTTACGAGTCGGAGGACGTCCGCTGGATGCTTTTGAATCTGTCGTGCATGAAATTCCGATGTTA
TCGCTCGATAACGCCTTTGATGATGGTGAATTAGAAAGCTTTTATCGCCGTATGACGGATCGGATCCAGGCGGTACAGCA
TAGTGCCTTTTGTTGTGAGCCGAAACTGGATGGCTTGGCGGTCAGTTTACTATACGAAAATGGTGTTCTGACCCGCGCGG
CGACTCGTGGTGATGGTATGACCGGCGAAAACATCACGGAAAATGTACGTACCATTAAAGCAATCCCTTTACGTCTGCAA
GGCGCAGATTTTCCAACTCGCCTTGAAGTGCGCGGTGAAGTGTTTATGCCTAAAGCGGGGTTTGAAGCACTTAACGCACG
AGCATTGAAAAAAGGTGAAAAGCAGTTTGTTAACCCTCGTAATGCGGCGGCGGGAAGCCTACGTCAACTCGACTCCAAAA
TTACCGCCCAACGTCCACTGGCTTTTTACGCTTATAGTGTTGGTGTTATTGAAGGCGGAGAGTTAGCGACCAGCCACTAT
CAACGATTTTTGCAACTAAAGGGCTGGGGGCTGCCGATTTGTCCTGAAACCAAACTGGTAACTTCTCTCGCCGAAGTCAA
AGCTTTTTATCAAGACATTTTGCAACGTCGTCAGTTTTTGGCTTATGAGATTGATGGCGTCGTCATTAAAGTTGACGATA
TCCAGTTGCAGGAGCGATTGGGGTTCGTTGCTAGAGCGCCGCGCTGGGCGATTGCCTATAAATTTCCTGCGCAGGAAGAG
CTTACTTTGCTCAATGATGTTGAATTTCAGGTAGGTCGTACTGGCGCGATTACCCCAGTTGCTAAGTTGGAGCCTGTTTT
TGTCGGTGGTGTTACGGTAAGTAATGCCACATTACATAATGCCGATGAGATTGAGCGTTTGGGAGTGATGGTCGGAGATA
CGGTGGTGATCCGCCGTGCTGGGGATGTCATCCCACAAATCGTCTCTGTGGTATTAGAGCGACGCCCTGAGAATGCAAAA
TCTATTGTATTTCCTACTCGTTGCCCAGTTTGTCAATCGGATGTTGAACGGGTTGAAGGAGAAGCGGTTGCTCGATGTTC
CGGTGGACTGATTTGCCAAGCTCAGCGCAAAGAGGCATTGAAGCATTTTGTTTCTCGCAAGGCCATGGATGTAGAAGGCT
TGGGCGATAAAGTGATTGAGCAACTGGTTGACCGAGAAATGGTGAGTACACCTGCTGATTTATTCCGGTTAAGAGCGGGA
GAGCTCACGATTTTGGAGCGAATGGGCCCCAAATCGGCGCAGAATGTGATTGATGCTCTCAATAAAGCGAAGCAAACCAC
ATTACCTAAGTTTTTATATGCACTGGGTATTCGTGAAGTCGGCGATGCTACCGCACTCAACTTGGCTCAGCACTTTTTAA
GTTTGGAAGCGATTCAACAAGCGAGTTTAGAACAATTTATCGAAGTTCCCGATGTCGGAGTGGTTGTTGCAAGCCATTTA
CTGGCCTTCTTTGCGCAAGACCGTAACCAACAAGTGATCAATGAATTGCTAGAACAAGGCATCACATGGCCGGCGCTAAC
GGCTGCGCCCGTGGCGGTAGACTCTGCTCTAGCGGGCAAAATTGTTGTGTTAACCGGCAGTTTTACTCAATTGTCTCGTA
ATGATGCGAAAGCCGCACTACAAGCTTTAGGTGCTAAGGTGACGGGTAGCGTATCAAAAAATACTGATATTGTTTTTGCA
GGAGAAGCAGCGGGCTCTAAGCTAGCCAAAGCGACCGAGTTGGGTATCCAAGTTTTCGATGAGCAAGCATTAATCGAATT
TTTGAAATAA

Upstream 100 bases:

>100_bases
GCGTGCTGCTTTACTTCGTCAGTAACAAGATAAAGATATCCCTAGTTATACTAAAAGGGCTCCGTAGCGGAGCCTTTTTT
CATCATCCGAGAGAATTACG

Downstream 100 bases:

>100_bases
GCCTATTCACTTAATATTTGAAGCGGCAGTGGTGTTGGCGATGTGTGTTCACTCCAACATCGCCTGCCTATGCTTATAGG
GATGAATTTACTTGCAGCCT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MSDVAQRLTELRKTLHEHGVRYYVEDAPTIPDAEYDRLMRELLELEAAHPELMSSDSPSLRVGGRPLDAFESVVHEIPML
SLDNAFDDGELESFYRRMTDRIQAVQHSAFCCEPKLDGLAVSLLYENGVLTRAATRGDGMTGENITENVRTIKAIPLRLQ
GADFPTRLEVRGEVFMPKAGFEALNARALKKGEKQFVNPRNAAAGSLRQLDSKITAQRPLAFYAYSVGVIEGGELATSHY
QRFLQLKGWGLPICPETKLVTSLAEVKAFYQDILQRRQFLAYEIDGVVIKVDDIQLQERLGFVARAPRWAIAYKFPAQEE
LTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIERLGVMVGDTVVIRRAGDVIPQIVSVVLERRPENAK
SIVFPTRCPVCQSDVERVEGEAVARCSGGLICQAQRKEALKHFVSRKAMDVEGLGDKVIEQLVDREMVSTPADLFRLRAG
ELTILERMGPKSAQNVIDALNKAKQTTLPKFLYALGIREVGDATALNLAQHFLSLEAIQQASLEQFIEVPDVGVVVASHL
LAFFAQDRNQQVINELLEQGITWPALTAAPVAVDSALAGKIVVLTGSFTQLSRNDAKAALQALGAKVTGSVSKNTDIVFA
GEAAGSKLAKATELGIQVFDEQALIEFLK

Sequences:

>Translated_669_residues
MSDVAQRLTELRKTLHEHGVRYYVEDAPTIPDAEYDRLMRELLELEAAHPELMSSDSPSLRVGGRPLDAFESVVHEIPML
SLDNAFDDGELESFYRRMTDRIQAVQHSAFCCEPKLDGLAVSLLYENGVLTRAATRGDGMTGENITENVRTIKAIPLRLQ
GADFPTRLEVRGEVFMPKAGFEALNARALKKGEKQFVNPRNAAAGSLRQLDSKITAQRPLAFYAYSVGVIEGGELATSHY
QRFLQLKGWGLPICPETKLVTSLAEVKAFYQDILQRRQFLAYEIDGVVIKVDDIQLQERLGFVARAPRWAIAYKFPAQEE
LTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIERLGVMVGDTVVIRRAGDVIPQIVSVVLERRPENAK
SIVFPTRCPVCQSDVERVEGEAVARCSGGLICQAQRKEALKHFVSRKAMDVEGLGDKVIEQLVDREMVSTPADLFRLRAG
ELTILERMGPKSAQNVIDALNKAKQTTLPKFLYALGIREVGDATALNLAQHFLSLEAIQQASLEQFIEVPDVGVVVASHL
LAFFAQDRNQQVINELLEQGITWPALTAAPVAVDSALAGKIVVLTGSFTQLSRNDAKAALQALGAKVTGSVSKNTDIVFA
GEAAGSKLAKATELGIQVFDEQALIEFLK
>Mature_668_residues
SDVAQRLTELRKTLHEHGVRYYVEDAPTIPDAEYDRLMRELLELEAAHPELMSSDSPSLRVGGRPLDAFESVVHEIPMLS
LDNAFDDGELESFYRRMTDRIQAVQHSAFCCEPKLDGLAVSLLYENGVLTRAATRGDGMTGENITENVRTIKAIPLRLQG
ADFPTRLEVRGEVFMPKAGFEALNARALKKGEKQFVNPRNAAAGSLRQLDSKITAQRPLAFYAYSVGVIEGGELATSHYQ
RFLQLKGWGLPICPETKLVTSLAEVKAFYQDILQRRQFLAYEIDGVVIKVDDIQLQERLGFVARAPRWAIAYKFPAQEEL
TLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIERLGVMVGDTVVIRRAGDVIPQIVSVVLERRPENAKS
IVFPTRCPVCQSDVERVEGEAVARCSGGLICQAQRKEALKHFVSRKAMDVEGLGDKVIEQLVDREMVSTPADLFRLRAGE
LTILERMGPKSAQNVIDALNKAKQTTLPKFLYALGIREVGDATALNLAQHFLSLEAIQQASLEQFIEVPDVGVVVASHLL
AFFAQDRNQQVINELLEQGITWPALTAAPVAVDSALAGKIVVLTGSFTQLSRNDAKAALQALGAKVTGSVSKNTDIVFAG
EAAGSKLAKATELGIQVFDEQALIEFLK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=65.6203288490284, Blast_Score=907, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=521, Percent_Identity=21.6890595009597, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_VIBC3 (A5F2W3)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F2W3
- STRING:   A5F2W3
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0492
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   VCHO345073:VC0395_A0492-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73372; Mature: 73241

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 173-173 BINDING 290-290 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVAQRLTELRKTLHEHGVRYYVEDAPTIPDAEYDRLMRELLELEAAHPELMSSDSPSL
CCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCE
RVGGRPLDAFESVVHEIPMLSLDNAFDDGELESFYRRMTDRIQAVQHSAFCCEPKLDGLA
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEE
VSLLYENGVLTRAATRGDGMTGENITENVRTIKAIPLRLQGADFPTRLEVRGEVFMPKAG
EEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCEEEECCCEECCCCC
FEALNARALKKGEKQFVNPRNAAAGSLRQLDSKITAQRPLAFYAYSVGVIEGGELATSHY
HHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHH
QRFLQLKGWGLPICPETKLVTSLAEVKAFYQDILQRRQFLAYEIDGVVIKVDDIQLQERL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCHHHHHHH
GFVARAPRWAIAYKFPAQEELTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHN
HHHHCCCCEEEEEECCCCCHHHHHHCCCCEECCCCCCCCHHHCCCEEECCEEECCCCCCC
ADEIERLGVMVGDTVVIRRAGDVIPQIVSVVLERRPENAKSIVFPTRCPVCQSDVERVEG
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHCC
EAVARCSGGLICQAQRKEALKHFVSRKAMDVEGLGDKVIEQLVDREMVSTPADLFRLRAG
HHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCC
ELTILERMGPKSAQNVIDALNKAKQTTLPKFLYALGIREVGDATALNLAQHFLSLEAIQQ
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ASLEQFIEVPDVGVVVASHLLAFFAQDRNQQVINELLEQGITWPALTAAPVAVDSALAGK
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHCCHHHHHHHHCCE
IVVLTGSFTQLSRNDAKAALQALGAKVTGSVSKNTDIVFAGEAAGSKLAKATELGIQVFD
EEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
EQALIEFLK
HHHHHHHHC
>Mature Secondary Structure 
SDVAQRLTELRKTLHEHGVRYYVEDAPTIPDAEYDRLMRELLELEAAHPELMSSDSPSL
CHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCE
RVGGRPLDAFESVVHEIPMLSLDNAFDDGELESFYRRMTDRIQAVQHSAFCCEPKLDGLA
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEE
VSLLYENGVLTRAATRGDGMTGENITENVRTIKAIPLRLQGADFPTRLEVRGEVFMPKAG
EEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCEEEECCCEECCCCC
FEALNARALKKGEKQFVNPRNAAAGSLRQLDSKITAQRPLAFYAYSVGVIEGGELATSHY
HHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHH
QRFLQLKGWGLPICPETKLVTSLAEVKAFYQDILQRRQFLAYEIDGVVIKVDDIQLQERL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCHHHHHHH
GFVARAPRWAIAYKFPAQEELTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHN
HHHHCCCCEEEEEECCCCCHHHHHHCCCCEECCCCCCCCHHHCCCEEECCEEECCCCCCC
ADEIERLGVMVGDTVVIRRAGDVIPQIVSVVLERRPENAKSIVFPTRCPVCQSDVERVEG
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHCC
EAVARCSGGLICQAQRKEALKHFVSRKAMDVEGLGDKVIEQLVDREMVSTPADLFRLRAG
HHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCC
ELTILERMGPKSAQNVIDALNKAKQTTLPKFLYALGIREVGDATALNLAQHFLSLEAIQQ
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ASLEQFIEVPDVGVVVASHLLAFFAQDRNQQVINELLEQGITWPALTAAPVAVDSALAGK
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHCCHHHHHHHHCCE
IVVLTGSFTQLSRNDAKAALQALGAKVTGSVSKNTDIVFAGEAAGSKLAKATELGIQVFD
EEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
EQALIEFLK
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA