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The map label for this gene is plsB

Identifier: 147675490

GI number: 147675490

Start: 2575890

End: 2578325

Strand: Reverse

Name: plsB

Synonym: VC0395_A2422

Alternate gene names: 147675490

Gene position: 2578325-2575890 (Counterclockwise)

Preceding gene: 147673083

Following gene: 147673890

Centisome position: 85.26

GC content: 51.6

Gene sequence:

>2436_bases
TTGAACTCTATGTCTTCAGGACACCTATTATCCCGTTCACTACTGAAGTTGCCGATGTCGGTGCTGGTGAAAGGGACGGC
GATTCCGTCTAATCCGATCCAAGATCTGGATATCGATACTCATAAACCGGTGATTTACGCCTTACCGTTTCGCTCCAACG
TCGACTTGCTGACGTTGCAAACACATGCCAAAGAAGCTGGGCTGCCTGACCCTTTAGAGCCTTTGATGCTGAATGGCAAA
GCTTTCCAGCGCTATGTGTTTATTGCTTCGCGCCCAACGCTGCTGAGTAGCGATCAACATGTGCCGAGTGACTCTATTGC
GCTGTTTTCTGAGCTGCTGACCGAACATAAGCTCGACAGTGAGTTGGATGTGCAGGTGATTCCAGCAACGGTTTTGTGGG
GACGTAAACCGGGCAAAGAAGGGCAAGAAAGACCCTATTTGCAAGCGTTGAATGGTCCGGAGAAAGCGCTGGCCGTTTTG
GCTTCTGGCCGTGACTGCTTGGTGCGTTTTAGCCCTGTGGTGTCGATGCGTTACATGGCGGATACCCACGGCACTGATGC
CTCGATCGCGCACAAATTGGCTCGTGTAGCACGCATTCACTTCTCACGGCAAAAACTGGCGGCGTCAGGCCCCAATTTGC
CGCAGCGAGCACAACTGTTTGCCCGCTTGATGAACTCTCCTGCGATTGAAAAAGCGATTGCGGACGAAGCGAAATCCAAA
CAGATCCCGCTTGAGAAAGCGCGCAAAGAAGCGCACGACATTCTGGATGAGATCGCGGCGGATTTTTCCTATTCGCTGGT
GAAAAAAGGCGATCGCATTTTAGGTTGGTTGTGGAACCGCATCTATCAAGGGTTGAACATCAATAACGCGGCCACCGTGC
GTCGCTTGGCGCAAGATGGCCATGAGATTGTCTACGTGCCTTGTCACCGTAGCCATATGGATTACTTACTGCTTTCTTAT
GTGCTTTATCATGAAGGCATGGTGCCGCCGCATATTGCTGCTGGGATCAACCTGAACTTTTTCCCTGCCGGGCCGATTTT
CCGCCGCGGCGGCGCGTTCTTCATTCGTCGCAGCTTTAAAGGTGCGCCTCTTTATTCAACGATTTTTCGTGAATATCTTG
CTGAGTTGTTTGCGAAAGGCTATTCGGTGGAATACTTCAGCGAAGGTGGCCGCTCGCGTACCGGTCGCTTGCTGCCAGCG
AAAACTGGCATGTTGGCGATGACCATTCAAGCTATGTTGCGTGGCCTGAATCGTCCAGTGACTTTGGTCCCAGTCTACAT
CGGCTATGAGCATGTGATGGAAGTAGGTACTTACGCCAAAGAGTTACGCGGCAAACGTAAAGAGAAAGAGAATGCGGGTT
TAGTGCTGCGCACGTTGCGTAAGCTGCGCAATTTTGGTCAAGGTTACGTGAACTTCGGTGAGCCGATCCCACTCAACCAG
TTTTTGAATGAAACCGTACCGCAATGGACGCAAGATATTGACCCGATGGGCGAGAGCAAGCCGCAATGGATGACGCCAAC
GGTGAATAAACTCGCGACCCGTATGATGACACACATCAACGATGCCGCGGCAGTGAATGCGATGACCTTGTGTGCGACGG
CGCTGCTGGCTTCCCGTCAGCGTGCCTTGGCGCGTGACAACCTGATTAAGCAGGTGGATTGTTACTTATCGCTGCTGCGC
AATGTGCCGTATTCAGCCACATCAACGCTGCCGAGTGAGAGTGCAGAAAAGCTGGTTGAACATGCGGAGTCGTTGGATAA
GTTTGTGGTGGAAACCGATACCATGGGCGACATTATTTCGCTGGATCGCAACCAATCGATCCTGATGACTTATTATCGCA
ACAACATTATCCACCTGTTGGCTCTGCCATCGCTGATCGCTCAGTTGTTGATTCGTCAGCAAAGCGTATCGCTGGAAAAG
GTGCAAGCGACCGTTGCACAAATCTATCCTTTCCTGAAACAAGAGCTGTTTTTGCGTTTTGAGGCAGAAGAACTCAACGA
TCTGGTACTGCGTTATGTGGCAGAGCTAGCACGCCAAGGTTTGGTCACGGTGGAAGGGAAAACCGTCACCCTGAATCAAG
CGCAAACTCAAGTGCTGATGCTGCTGGGGCGCATTATCTCCGAAACACTGCAGCGTTATGCGATTGCGCTGAACCTGCTG
GTGAGCTGCCCACATTTGGGTAAAGCAGAGCTGGAAGAGAAGAGTCAGGAAGTGGCGCAGCGTCTTGGTCGTTTGCATGG
CATTAATGCGCCTGAGTTCTTTGACAAAGGGGTATTTGCTTCGCTATTCGTGACGTTGCAAGAGCAGGGATATCTCGATG
ATCAGGGGCGTTGCGTATTAGAAACAGCGAAGCCGCTCTCTCGCCAGCTTTATGCGCTGATCTACCCAGAAGTGCGCATG
ACGATTCAAGAAAGCTTGTGTCAGGTTGACGCGTAA

Upstream 100 bases:

>100_bases
TATTCTTGGCGCGCAAAAAACGATACCGCACGATGGTGAGCTCTCGCGTTGGCCGTGAAAGCAGACGTACTGTCGCGCTT
ATTTCAAGAATCTTGAGGCT

Downstream 100 bases:

>100_bases
CGATTGTGTTCTAGCCAATCGAACCGAATGAAAAAGGCATCCTGCGGATGCCTTTTCTTTTGCCTTAAGCTGGGATTACC
AAACGCTGAGCAGAATGCCT

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT

Number of amino acids: Translated: 811; Mature: 811

Protein sequence:

>811_residues
MNSMSSGHLLSRSLLKLPMSVLVKGTAIPSNPIQDLDIDTHKPVIYALPFRSNVDLLTLQTHAKEAGLPDPLEPLMLNGK
AFQRYVFIASRPTLLSSDQHVPSDSIALFSELLTEHKLDSELDVQVIPATVLWGRKPGKEGQERPYLQALNGPEKALAVL
ASGRDCLVRFSPVVSMRYMADTHGTDASIAHKLARVARIHFSRQKLAASGPNLPQRAQLFARLMNSPAIEKAIADEAKSK
QIPLEKARKEAHDILDEIAADFSYSLVKKGDRILGWLWNRIYQGLNINNAATVRRLAQDGHEIVYVPCHRSHMDYLLLSY
VLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGAPLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPA
KTGMLAMTIQAMLRGLNRPVTLVPVYIGYEHVMEVGTYAKELRGKRKEKENAGLVLRTLRKLRNFGQGYVNFGEPIPLNQ
FLNETVPQWTQDIDPMGESKPQWMTPTVNKLATRMMTHINDAAAVNAMTLCATALLASRQRALARDNLIKQVDCYLSLLR
NVPYSATSTLPSESAEKLVEHAESLDKFVVETDTMGDIISLDRNQSILMTYYRNNIIHLLALPSLIAQLLIRQQSVSLEK
VQATVAQIYPFLKQELFLRFEAEELNDLVLRYVAELARQGLVTVEGKTVTLNQAQTQVLMLLGRIISETLQRYAIALNLL
VSCPHLGKAELEEKSQEVAQRLGRLHGINAPEFFDKGVFASLFVTLQEQGYLDDQGRCVLETAKPLSRQLYALIYPEVRM
TIQESLCQVDA

Sequences:

>Translated_811_residues
MNSMSSGHLLSRSLLKLPMSVLVKGTAIPSNPIQDLDIDTHKPVIYALPFRSNVDLLTLQTHAKEAGLPDPLEPLMLNGK
AFQRYVFIASRPTLLSSDQHVPSDSIALFSELLTEHKLDSELDVQVIPATVLWGRKPGKEGQERPYLQALNGPEKALAVL
ASGRDCLVRFSPVVSMRYMADTHGTDASIAHKLARVARIHFSRQKLAASGPNLPQRAQLFARLMNSPAIEKAIADEAKSK
QIPLEKARKEAHDILDEIAADFSYSLVKKGDRILGWLWNRIYQGLNINNAATVRRLAQDGHEIVYVPCHRSHMDYLLLSY
VLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGAPLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPA
KTGMLAMTIQAMLRGLNRPVTLVPVYIGYEHVMEVGTYAKELRGKRKEKENAGLVLRTLRKLRNFGQGYVNFGEPIPLNQ
FLNETVPQWTQDIDPMGESKPQWMTPTVNKLATRMMTHINDAAAVNAMTLCATALLASRQRALARDNLIKQVDCYLSLLR
NVPYSATSTLPSESAEKLVEHAESLDKFVVETDTMGDIISLDRNQSILMTYYRNNIIHLLALPSLIAQLLIRQQSVSLEK
VQATVAQIYPFLKQELFLRFEAEELNDLVLRYVAELARQGLVTVEGKTVTLNQAQTQVLMLLGRIISETLQRYAIALNLL
VSCPHLGKAELEEKSQEVAQRLGRLHGINAPEFFDKGVFASLFVTLQEQGYLDDQGRCVLETAKPLSRQLYALIYPEVRM
TIQESLCQVDA
>Mature_811_residues
MNSMSSGHLLSRSLLKLPMSVLVKGTAIPSNPIQDLDIDTHKPVIYALPFRSNVDLLTLQTHAKEAGLPDPLEPLMLNGK
AFQRYVFIASRPTLLSSDQHVPSDSIALFSELLTEHKLDSELDVQVIPATVLWGRKPGKEGQERPYLQALNGPEKALAVL
ASGRDCLVRFSPVVSMRYMADTHGTDASIAHKLARVARIHFSRQKLAASGPNLPQRAQLFARLMNSPAIEKAIADEAKSK
QIPLEKARKEAHDILDEIAADFSYSLVKKGDRILGWLWNRIYQGLNINNAATVRRLAQDGHEIVYVPCHRSHMDYLLLSY
VLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGAPLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPA
KTGMLAMTIQAMLRGLNRPVTLVPVYIGYEHVMEVGTYAKELRGKRKEKENAGLVLRTLRKLRNFGQGYVNFGEPIPLNQ
FLNETVPQWTQDIDPMGESKPQWMTPTVNKLATRMMTHINDAAAVNAMTLCATALLASRQRALARDNLIKQVDCYLSLLR
NVPYSATSTLPSESAEKLVEHAESLDKFVVETDTMGDIISLDRNQSILMTYYRNNIIHLLALPSLIAQLLIRQQSVSLEK
VQATVAQIYPFLKQELFLRFEAEELNDLVLRYVAELARQGLVTVEGKTVTLNQAQTQVLMLLGRIISETLQRYAIALNLL
VSCPHLGKAELEEKSQEVAQRLGRLHGINAPEFFDKGVFASLFVTLQEQGYLDDQGRCVLETAKPLSRQLYALIYPEVRM
TIQESLCQVDA

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family

Homologues:

Organism=Homo sapiens, GI7657134, Length=526, Percent_Identity=25.2851711026616, Blast_Score=153, Evalue=7e-37,
Organism=Homo sapiens, GI190358539, Length=282, Percent_Identity=29.4326241134752, Blast_Score=124, Evalue=4e-28,
Organism=Escherichia coli, GI87082362, Length=804, Percent_Identity=56.9651741293532, Blast_Score=927, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=350, Percent_Identity=28.8571428571429, Blast_Score=151, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71988723, Length=474, Percent_Identity=25.7383966244726, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71988728, Length=414, Percent_Identity=26.8115942028986, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17864692, Length=293, Percent_Identity=33.7883959044369, Blast_Score=161, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21357731, Length=319, Percent_Identity=28.2131661442006, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24650754, Length=319, Percent_Identity=28.2131661442006, Blast_Score=124, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24650752, Length=319, Percent_Identity=28.2131661442006, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSB_VIBC3 (A5F4F5)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4F5
- STRING:   A5F4F5
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2422
- eggNOG:   COG2937
- HOGENOM:   HBG296590
- OMA:   VIYVPCH
- ProtClustDB:   PRK04974
- BioCyc:   VCHO345073:VC0395_A2422-MONOMER
- HAMAP:   MF_00393
- InterPro:   IPR002123
- InterPro:   IPR022284
- SMART:   SM00563
- TIGRFAMs:   TIGR03703

Pfam domain/function: PF01553 Acyltransferase

EC number: =2.3.1.15

Molecular weight: Translated: 90813; Mature: 90813

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSMSSGHLLSRSLLKLPMSVLVKGTAIPSNPIQDLDIDTHKPVIYALPFRSNVDLLTLQ
CCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEE
THAKEAGLPDPLEPLMLNGKAFQRYVFIASRPTLLSSDQHVPSDSIALFSELLTEHKLDS
HHHHHCCCCCCCHHHHCCCHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
ELDVQVIPATVLWGRKPGKEGQERPYLQALNGPEKALAVLASGRDCLVRFSPVVSMRYMA
CCCEEEECHHEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHEEEECHHHHHHHHH
DTHGTDASIAHKLARVARIHFSRQKLAASGPNLPQRAQLFARLMNSPAIEKAIADEAKSK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCC
QIPLEKARKEAHDILDEIAADFSYSLVKKGDRILGWLWNRIYQGLNINNAATVRRLAQDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
HEIVYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFK
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCCEEEEECCC
GAPLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTGMLAMTIQAMLRGLNRPV
CCCHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
TLVPVYIGYEHVMEVGTYAKELRGKRKEKENAGLVLRTLRKLRNFGQGYVNFGEPIPLNQ
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHH
FLNETVPQWTQDIDPMGESKPQWMTPTVNKLATRMMTHINDAAAVNAMTLCATALLASRQ
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RALARDNLIKQVDCYLSLLRNVPYSATSTLPSESAEKLVEHAESLDKFVVETDTMGDIIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHC
LDRNQSILMTYYRNNIIHLLALPSLIAQLLIRQQSVSLEKVQATVAQIYPFLKQELFLRF
CCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
EAEELNDLVLRYVAELARQGLVTVEGKTVTLNQAQTQVLMLLGRIISETLQRYAIALNLL
CHHHHHHHHHHHHHHHHHCCCEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSCPHLGKAELEEKSQEVAQRLGRLHGINAPEFFDKGVFASLFVTLQEQGYLDDQGRCVL
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHCCCCCCCCCEEE
ETAKPLSRQLYALIYPEVRMTIQESLCQVDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSMSSGHLLSRSLLKLPMSVLVKGTAIPSNPIQDLDIDTHKPVIYALPFRSNVDLLTLQ
CCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEE
THAKEAGLPDPLEPLMLNGKAFQRYVFIASRPTLLSSDQHVPSDSIALFSELLTEHKLDS
HHHHHCCCCCCCHHHHCCCHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
ELDVQVIPATVLWGRKPGKEGQERPYLQALNGPEKALAVLASGRDCLVRFSPVVSMRYMA
CCCEEEECHHEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHEEEECHHHHHHHHH
DTHGTDASIAHKLARVARIHFSRQKLAASGPNLPQRAQLFARLMNSPAIEKAIADEAKSK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCC
QIPLEKARKEAHDILDEIAADFSYSLVKKGDRILGWLWNRIYQGLNINNAATVRRLAQDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
HEIVYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFK
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCCEEEEECCC
GAPLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTGMLAMTIQAMLRGLNRPV
CCCHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
TLVPVYIGYEHVMEVGTYAKELRGKRKEKENAGLVLRTLRKLRNFGQGYVNFGEPIPLNQ
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHH
FLNETVPQWTQDIDPMGESKPQWMTPTVNKLATRMMTHINDAAAVNAMTLCATALLASRQ
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RALARDNLIKQVDCYLSLLRNVPYSATSTLPSESAEKLVEHAESLDKFVVETDTMGDIIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHC
LDRNQSILMTYYRNNIIHLLALPSLIAQLLIRQQSVSLEKVQATVAQIYPFLKQELFLRF
CCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
EAEELNDLVLRYVAELARQGLVTVEGKTVTLNQAQTQVLMLLGRIISETLQRYAIALNLL
CHHHHHHHHHHHHHHHHHCCCEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSCPHLGKAELEEKSQEVAQRLGRLHGINAPEFFDKGVFASLFVTLQEQGYLDDQGRCVL
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHCCCCCCCCCEEE
ETAKPLSRQLYALIYPEVRMTIQESLCQVDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA