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The map label for this gene is topB

Identifier: 147675446

GI number: 147675446

Start: 1753670

End: 1755613

Strand: Reverse

Name: topB

Synonym: VC0395_A1630

Alternate gene names: 147675446

Gene position: 1755613-1753670 (Counterclockwise)

Preceding gene: 147673099

Following gene: 147674598

Centisome position: 58.05

GC content: 49.13

Gene sequence:

>1944_bases
ATGACCCGGTTATTTATTGCAGAAAAACCTAGCCTTGCGCGAGCGATTGCCGATGCACTACCCAAGCCTCATAAAAAAGA
ACAAGGGTGTATTCGCTGCGCTAATGGCGATATCGTGACTTGGTGCATTGGTCACCTTCTCGAACAAGTCGAGCCAGACG
CTTATGATGAGCGATACAAAAAGTGGAATATGGCGGATTTGCCTATCATTCCAGAGCAGTGGCAACTGCGCCCAAGAAAA
TCCTCCTCTCAACAGCTCACCGTCGTTCGTAAGCTACTCAAAGAAGCAACGCAGATTATCCATGCCGGTGACCCCGATCG
CGAAGGGCAACTGTTGGTGGATGAGGTGATCGATTATTGCAAAGTGCCGAAGAGCAAAAAAGAAACCGTGCAGCGCCTTT
TGATCAGTGATTTAAACCTTTCTGCAGTCAAGCGTGCCTTACAAGGCTTGCGCAGTAATCGAGAGTTTATTCCACTCTCT
GTTTCGGCTTTAGCGCGTTCACGCGCTGACTGGCTGTATGGTATGAATATGTCTCGAGCTTATACCCTGCTTGGGAAAAA
AGCCGGTTATCAAGGCGTGCTCTCCGTAGGGCGAGTACAGACGCCAGTACTGGGTTTAGTGGTGCGACGGGATGAAGAGA
TCGAACATTTTATCCCGCATGATTATTTTACTCTTGATGCTTTAATTCCATACCAAAATGGCACCGAATACTTCGACATA
CGTGCCCGTTGGAAACCAAGTGAGGCCTGCTTACCTTGGCAAGATGAAGAAGGGCGGGTCACTAACCGTAAATTGGTGGA
CAATGTTGCCAGTCGCATTGCGAATCAGCCCGCGACTGTGACTGAATCAGAGCAAGATCAAACCAAGCAAGCGGCCCCTT
TGCCCTATTCACTTTCAGCGCTGCAAATTGATGCGGCAAAACGGTATAACTTCAGCGCACAACAAGTGTTGGATCTGTGC
CAGTCTCTTTATGAAAAGCATAAATTGATCACTTATCCGCGATCTGATTGCCGATACTTACCGAAAGAGCATTTAGCTCA
GGCTCCCGACGTGGTAGCGGCAATAGCCAACAATGCGCAAGAGATGGTCACCGCAGTGAATGATGCGGATTTATCACTAC
GCTCCAAAGCGTGGAATGACAGTAAAGTCGACGCGCACCACGCCATCATTCCAACGCCGAAGAAAGCGTCGGTTAATGCG
CTGTCAGGGCATGAAATGAAAGTCTATCAACTGATTGCGCGTCAGTACCTTATGCAATTTTATCCTGCGGCGATTTATGC
AGAAGCCAAACTGGTTTTTAACATCGCTGGCGGCATTTTTATTGCCAAAGGGCGTCAACTGCTCAGTGCTGGATGGAAAG
TGTTGACCGGTCAGCAAGATGAGCAAGAAGAGTGCGTGGATAAAGTCCCCCCTTTGCCTGTTGGCATTGTGCTGCAGTGT
CGAGAAGGGGAAATAAAACAGAGACAAACCGAGCCGCCACGCCATTTTACGGAAGCTACATTACTGCAGGCGATGACGGG
GATTGCCCGTTTCGTGGCCGATAAAGAGCTGAAAAAAATTCTGCGTGAGACGGATGGTTTAGGCACTGAAGCCACACGAG
CTGGGATCTTGGATACCTTGTTTAAGCGTGGATTACTGCAGCGTGATAACAAGCTCATTAAAAGCACGCCAGCTGGCCGA
GGTTTGATTCATGCGTTACCCAGTGAAGCCACCTATCCCGATATGACGGCACATTGGGAACATCAACTGCAAGCGATCGC
GGAAAAGGGGCAAGCTTATCAACCCTTTATGCAGACACTACAAGGCCGCCTCGAGCAGTTAATTGAGCAAGTTCAGGTCG
CACCGGTTCCTGTATCATTGCAAGCTTTGCCAGCGGTGAGTAAACCTGCCTTTAAGCGCACACGCCGTGCACCAAAGAGT
AAAGCCAGAACGTATAAAGCTTAA

Upstream 100 bases:

>100_bases
AGCAAGCCAAGAGTAATACTGGTTAAATTTACAGTTTTCCTTGATTTCATAAGGCCACATTGAGTAATGTGGCCTTAATT
TTATCTCGTCAGCGATTTCC

Downstream 100 bases:

>100_bases
TAAACCCAGAGCAGAGCAAAATGATCCCACTGATTTACCATCCTATTTATTCACAACTGGATTTGCCTGTGGGGCATCGT
TATCCGATCAATAAGTATCG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III

Number of amino acids: Translated: 647; Mature: 646

Protein sequence:

>647_residues
MTRLFIAEKPSLARAIADALPKPHKKEQGCIRCANGDIVTWCIGHLLEQVEPDAYDERYKKWNMADLPIIPEQWQLRPRK
SSSQQLTVVRKLLKEATQIIHAGDPDREGQLLVDEVIDYCKVPKSKKETVQRLLISDLNLSAVKRALQGLRSNREFIPLS
VSALARSRADWLYGMNMSRAYTLLGKKAGYQGVLSVGRVQTPVLGLVVRRDEEIEHFIPHDYFTLDALIPYQNGTEYFDI
RARWKPSEACLPWQDEEGRVTNRKLVDNVASRIANQPATVTESEQDQTKQAAPLPYSLSALQIDAAKRYNFSAQQVLDLC
QSLYEKHKLITYPRSDCRYLPKEHLAQAPDVVAAIANNAQEMVTAVNDADLSLRSKAWNDSKVDAHHAIIPTPKKASVNA
LSGHEMKVYQLIARQYLMQFYPAAIYAEAKLVFNIAGGIFIAKGRQLLSAGWKVLTGQQDEQEECVDKVPPLPVGIVLQC
REGEIKQRQTEPPRHFTEATLLQAMTGIARFVADKELKKILRETDGLGTEATRAGILDTLFKRGLLQRDNKLIKSTPAGR
GLIHALPSEATYPDMTAHWEHQLQAIAEKGQAYQPFMQTLQGRLEQLIEQVQVAPVPVSLQALPAVSKPAFKRTRRAPKS
KARTYKA

Sequences:

>Translated_647_residues
MTRLFIAEKPSLARAIADALPKPHKKEQGCIRCANGDIVTWCIGHLLEQVEPDAYDERYKKWNMADLPIIPEQWQLRPRK
SSSQQLTVVRKLLKEATQIIHAGDPDREGQLLVDEVIDYCKVPKSKKETVQRLLISDLNLSAVKRALQGLRSNREFIPLS
VSALARSRADWLYGMNMSRAYTLLGKKAGYQGVLSVGRVQTPVLGLVVRRDEEIEHFIPHDYFTLDALIPYQNGTEYFDI
RARWKPSEACLPWQDEEGRVTNRKLVDNVASRIANQPATVTESEQDQTKQAAPLPYSLSALQIDAAKRYNFSAQQVLDLC
QSLYEKHKLITYPRSDCRYLPKEHLAQAPDVVAAIANNAQEMVTAVNDADLSLRSKAWNDSKVDAHHAIIPTPKKASVNA
LSGHEMKVYQLIARQYLMQFYPAAIYAEAKLVFNIAGGIFIAKGRQLLSAGWKVLTGQQDEQEECVDKVPPLPVGIVLQC
REGEIKQRQTEPPRHFTEATLLQAMTGIARFVADKELKKILRETDGLGTEATRAGILDTLFKRGLLQRDNKLIKSTPAGR
GLIHALPSEATYPDMTAHWEHQLQAIAEKGQAYQPFMQTLQGRLEQLIEQVQVAPVPVSLQALPAVSKPAFKRTRRAPKS
KARTYKA
>Mature_646_residues
TRLFIAEKPSLARAIADALPKPHKKEQGCIRCANGDIVTWCIGHLLEQVEPDAYDERYKKWNMADLPIIPEQWQLRPRKS
SSQQLTVVRKLLKEATQIIHAGDPDREGQLLVDEVIDYCKVPKSKKETVQRLLISDLNLSAVKRALQGLRSNREFIPLSV
SALARSRADWLYGMNMSRAYTLLGKKAGYQGVLSVGRVQTPVLGLVVRRDEEIEHFIPHDYFTLDALIPYQNGTEYFDIR
ARWKPSEACLPWQDEEGRVTNRKLVDNVASRIANQPATVTESEQDQTKQAAPLPYSLSALQIDAAKRYNFSAQQVLDLCQ
SLYEKHKLITYPRSDCRYLPKEHLAQAPDVVAAIANNAQEMVTAVNDADLSLRSKAWNDSKVDAHHAIIPTPKKASVNAL
SGHEMKVYQLIARQYLMQFYPAAIYAEAKLVFNIAGGIFIAKGRQLLSAGWKVLTGQQDEQEECVDKVPPLPVGIVLQCR
EGEIKQRQTEPPRHFTEATLLQAMTGIARFVADKELKKILRETDGLGTEATRAGILDTLFKRGLLQRDNKLIKSTPAGRG
LIHALPSEATYPDMTAHWEHQLQAIAEKGQAYQPFMQTLQGRLEQLIEQVQVAPVPVSLQALPAVSKPAFKRTRRAPKSK
ARTYKA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI4507635, Length=664, Percent_Identity=23.3433734939759, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1788061, Length=618, Percent_Identity=61.0032362459547, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=558, Percent_Identity=24.5519713261649, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32563869, Length=622, Percent_Identity=23.1511254019293, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323263, Length=397, Percent_Identity=24.6851385390428, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24585251, Length=631, Percent_Identity=26.3074484944533, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640096, Length=624, Percent_Identity=22.1153846153846, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP3_VIBCH (Q9KQF5)

Other databases:

- EMBL:   AE003852
- PIR:   E82126
- RefSeq:   NP_231677.1
- ProteinModelPortal:   Q9KQF5
- SMR:   Q9KQF5
- GeneID:   2613424
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2043
- TIGR:   VC_2043
- HOGENOM:   HBG585507
- OMA:   DTLFKRQ
- ProtClustDB:   PRK07726
- BRENDA:   5.99.1.2
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:2.70.20.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01056

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 72711; Mature: 72580

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 332-332

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLFIAEKPSLARAIADALPKPHKKEQGCIRCANGDIVTWCIGHLLEQVEPDAYDERYK
CCEEEECCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHH
KWNMADLPIIPEQWQLRPRKSSSQQLTVVRKLLKEATQIIHAGDPDREGQLLVDEVIDYC
CCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
KVPKSKKETVQRLLISDLNLSAVKRALQGLRSNREFIPLSVSALARSRADWLYGMNMSRA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
YTLLGKKAGYQGVLSVGRVQTPVLGLVVRRDEEIEHFIPHDYFTLDALIPYQNGTEYFDI
HHHHHHCCCCHHHHHHCCHHCCHHHHHCCCCHHHHHHCCCCCEEEEEEEECCCCCCEEEE
RARWKPSEACLPWQDEEGRVTNRKLVDNVASRIANQPATVTESEQDQTKQAAPLPYSLSA
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHH
LQIDAAKRYNFSAQQVLDLCQSLYEKHKLITYPRSDCRYLPKEHLAQAPDVVAAIANNAQ
EEECHHHHCCCCHHHHHHHHHHHHHCCCEEECCCHHHCCCCHHHHHCCHHHHHHHHCCHH
EMVTAVNDADLSLRSKAWNDSKVDAHHAIIPTPKKASVNALSGHEMKVYQLIARQYLMQF
HHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
YPAAIYAEAKLVFNIAGGIFIAKGRQLLSAGWKVLTGQQDEQEECVDKVPPLPVGIVLQC
HHHHHHHHHEEEEEECCCEEEECCHHHHHCCCCEECCCCCCHHHHHHCCCCCCCCEEEEE
REGEIKQRQTEPPRHFTEATLLQAMTGIARFVADKELKKILRETDGLGTEATRAGILDTL
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FKRGLLQRDNKLIKSTPAGRGLIHALPSEATYPDMTAHWEHQLQAIAEKGQAYQPFMQTL
HHHHHHHHCCHHHHCCCCCCCHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
QGRLEQLIEQVQVAPVPVSLQALPAVSKPAFKRTRRAPKSKARTYKA
HHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure 
TRLFIAEKPSLARAIADALPKPHKKEQGCIRCANGDIVTWCIGHLLEQVEPDAYDERYK
CEEEECCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHH
KWNMADLPIIPEQWQLRPRKSSSQQLTVVRKLLKEATQIIHAGDPDREGQLLVDEVIDYC
CCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
KVPKSKKETVQRLLISDLNLSAVKRALQGLRSNREFIPLSVSALARSRADWLYGMNMSRA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
YTLLGKKAGYQGVLSVGRVQTPVLGLVVRRDEEIEHFIPHDYFTLDALIPYQNGTEYFDI
HHHHHHCCCCHHHHHHCCHHCCHHHHHCCCCHHHHHHCCCCCEEEEEEEECCCCCCEEEE
RARWKPSEACLPWQDEEGRVTNRKLVDNVASRIANQPATVTESEQDQTKQAAPLPYSLSA
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHH
LQIDAAKRYNFSAQQVLDLCQSLYEKHKLITYPRSDCRYLPKEHLAQAPDVVAAIANNAQ
EEECHHHHCCCCHHHHHHHHHHHHHCCCEEECCCHHHCCCCHHHHHCCHHHHHHHHCCHH
EMVTAVNDADLSLRSKAWNDSKVDAHHAIIPTPKKASVNALSGHEMKVYQLIARQYLMQF
HHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
YPAAIYAEAKLVFNIAGGIFIAKGRQLLSAGWKVLTGQQDEQEECVDKVPPLPVGIVLQC
HHHHHHHHHEEEEEECCCEEEECCHHHHHCCCCEECCCCCCHHHHHHCCCCCCCCEEEEE
REGEIKQRQTEPPRHFTEATLLQAMTGIARFVADKELKKILRETDGLGTEATRAGILDTL
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FKRGLLQRDNKLIKSTPAGRGLIHALPSEATYPDMTAHWEHQLQAIAEKGQAYQPFMQTL
HHHHHHHHCCHHHHCCCCCCCHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
QGRLEQLIEQVQVAPVPVSLQALPAVSKPAFKRTRRAPKSKARTYKA
HHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301