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The map label for this gene is cpsF [H]

Identifier: 147675424

GI number: 147675424

Start: 480907

End: 481659

Strand: Direct

Name: cpsF [H]

Synonym: VC0395_A0452

Alternate gene names: 147675424

Gene position: 480907-481659 (Clockwise)

Preceding gene: 147674949

Following gene: 147673097

Centisome position: 15.9

GC content: 46.61

Gene sequence:

>753_bases
ATGAGGAATAACCGATTGAAACGCATTAATTTTCTGGGTAGCCCGATGGATGTGGCTTCGATGAGTGAAACGGTTGCGCT
GATCCGCGATCGCATTTTGCGCCAAGAGTTTACCCAGCACGTGGTGGTCAATGTCGCTAAGTTGGTCAATATGCGTAAGG
ATCCTGAATTGGCTCAGTCCGTGACCGAGTGTGACATTATCAATATCGATGGTATGGGGGTGGTTTGGGGGGCACGCTTT
TTAGGTCACCAGGTTCCCGAACGAGTGGCGGGCGTTGACTTATTTCTTGAGCTCAATGCAATGGCCGCACAAGAACAAAT
GCCCGTATTCTACCTCGGTGCAAAGCCGGAGATTGTTGAAAAAACGGCAGCAGTGATGCAGGCAAAGCACCCACAATTAG
TGATCGCTGGTTATCACCACGGTTATTTTTGGGATGATGAGCAAGCGATAGTTGAAAAAATCCGTCAGTCAGGTGCAAAA
CTTCTGTTTGTGGCGATCACATCCCCGAAAAAAGAGAACTTTATCAACCGCTGGCAAGAGCAGTTAGGGGTGTTATTTGT
GATGGGGGTTGGCGGGACATTTGATGTTGTCGCTGGTCATGTTTCTCGGGCTCCCTTATGGATGCAAAAAGCCGGATTGG
AATGGCTATATCGTGTGTTACAAGAACCCGGAAGAATGTGGAAGCGTTATTTAATTACTAATAGCCGCTATGCTTGGTTA
TTGCTCAATGAAAAAATAAAACCAAAGAATTAA

Upstream 100 bases:

>100_bases
GTCTATCAACGTAACAAACGTTGGGTTAACCGAATTCACTACGCGCGCTGGACACAGGCGTGGATGTACCTTTCATTTCG
TTTTTATTTAAGAATTACCA

Downstream 100 bases:

>100_bases
CCTTTGGGTGAAAAAATAAACTTTGGTTTATTTTTGCTTTCTGGTTTTTATTCGTTGAATCAAGTTTTTTTAATATTATT
AATGCTTAGCCAATGCAATT

Product: UDP-N-acetyl-D-mannosamine transferase

Products: C55-PP-GlcNAc-ManNAcA; UDP [C]

Alternate protein names: UDP-ManNAcA transferase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRNNRLKRINFLGSPMDVASMSETVALIRDRILRQEFTQHVVVNVAKLVNMRKDPELAQSVTECDIINIDGMGVVWGARF
LGHQVPERVAGVDLFLELNAMAAQEQMPVFYLGAKPEIVEKTAAVMQAKHPQLVIAGYHHGYFWDDEQAIVEKIRQSGAK
LLFVAITSPKKENFINRWQEQLGVLFVMGVGGTFDVVAGHVSRAPLWMQKAGLEWLYRVLQEPGRMWKRYLITNSRYAWL
LLNEKIKPKN

Sequences:

>Translated_250_residues
MRNNRLKRINFLGSPMDVASMSETVALIRDRILRQEFTQHVVVNVAKLVNMRKDPELAQSVTECDIINIDGMGVVWGARF
LGHQVPERVAGVDLFLELNAMAAQEQMPVFYLGAKPEIVEKTAAVMQAKHPQLVIAGYHHGYFWDDEQAIVEKIRQSGAK
LLFVAITSPKKENFINRWQEQLGVLFVMGVGGTFDVVAGHVSRAPLWMQKAGLEWLYRVLQEPGRMWKRYLITNSRYAWL
LLNEKIKPKN
>Mature_250_residues
MRNNRLKRINFLGSPMDVASMSETVALIRDRILRQEFTQHVVVNVAKLVNMRKDPELAQSVTECDIINIDGMGVVWGARF
LGHQVPERVAGVDLFLELNAMAAQEQMPVFYLGAKPEIVEKTAAVMQAKHPQLVIAGYHHGYFWDDEQAIVEKIRQSGAK
LLFVAITSPKKENFINRWQEQLGVLFVMGVGGTFDVVAGHVSRAPLWMQKAGLEWLYRVLQEPGRMWKRYLITNSRYAWL
LLNEKIKPKN

Specific function: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II), the second lipid-linked intermediate involved in ECA synthesis [H]

COG id: COG1922

COG function: function code M; Teichoic acid biosynthesis proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 26 family [H]

Homologues:

Organism=Escherichia coli, GI2367289, Length=189, Percent_Identity=39.1534391534392, Blast_Score=121, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023085
- InterPro:   IPR004629 [H]

Pfam domain/function: PF03808 Glyco_tran_WecB [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 28544; Mature: 28544

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNNRLKRINFLGSPMDVASMSETVALIRDRILRQEFTQHVVVNVAKLVNMRKDPELAQS
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VTECDIINIDGMGVVWGARFLGHQVPERVAGVDLFLELNAMAAQEQMPVFYLGAKPEIVE
HHHCCEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHCCCEEEECCCCHHHH
KTAAVMQAKHPQLVIAGYHHGYFWDDEQAIVEKIRQSGAKLLFVAITSPKKENFINRWQE
HHHHHHHCCCCCEEEEEECCCEEECCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
QLGVLFVMGVGGTFDVVAGHVSRAPLWMQKAGLEWLYRVLQEPGRMWKRYLITNSRYAWL
HHCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LLNEKIKPKN
EECCCCCCCC
>Mature Secondary Structure
MRNNRLKRINFLGSPMDVASMSETVALIRDRILRQEFTQHVVVNVAKLVNMRKDPELAQS
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VTECDIINIDGMGVVWGARFLGHQVPERVAGVDLFLELNAMAAQEQMPVFYLGAKPEIVE
HHHCCEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHCCCEEEECCCCHHHH
KTAAVMQAKHPQLVIAGYHHGYFWDDEQAIVEKIRQSGAKLLFVAITSPKKENFINRWQE
HHHHHHHCCCCCEEEEEECCCEEECCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
QLGVLFVMGVGGTFDVVAGHVSRAPLWMQKAGLEWLYRVLQEPGRMWKRYLITNSRYAWL
HHCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LLNEKIKPKN
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosaminuronic-acid; undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate [C]

Specific reaction: UDP-N-acetyl-D-mannosaminuronic-acid + undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate = C55-PP-GlcNAc-ManNAcA + UDP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA