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The map label for this gene is glnB-1 [H]

Identifier: 147675413

GI number: 147675413

Start: 127959

End: 128324

Strand: Direct

Name: glnB-1 [H]

Synonym: VC0395_A0135

Alternate gene names: 147675413

Gene position: 127959-128324 (Clockwise)

Preceding gene: 147673206

Following gene: 147673697

Centisome position: 4.23

GC content: 45.9

Gene sequence:

>366_bases
ATGGACGACTGCTTCAGAGAGGAAGAGATGAAACTGATCAACGCAATCATTAAACCATTCAAATTGGATGATGTTCGCGA
AGCGCTGGCGGATGTTGGGATTGAGGGGATGACGGTGTCTGAAGTGAAAGGCTTTGGACGTCAAAAAGGACACACTGAAC
TGTACCGAGGTGCTGAGTATCAGGTCGATTTTCTCCCTAAAGTCAAAATTGAAATTGCGACTCAAAGCGACAATGTCGAT
CGCGTAGTTGAAGCGATCATTAAGGCGGCACACACCGGAAAAATCGGTGATGGCAAAATTTTTGTGTATGACCTAAGCCA
AGCGGTACGTATCCGTACTGGCGAAATGGATTCAGAAGCCCTTTAA

Upstream 100 bases:

>100_bases
ATCGGTGCAGCCTTTTGTTTTTTTACTCAGCAAATGCCTATATATCCATAAAAATCATTAAGTTAAAAAGTTGGCACGCC
CCTTGGATAATGAAGAGTAA

Downstream 100 bases:

>100_bases
ATTCAAGGATGAGATTTGATTATGGAACTATCAATCACAGTTACTGAGCTGCGTTACGCACTCGATACATTTTTCTTCCT
GATCTCAGGAGCGCTCGTCA

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 121; Mature: 121

Protein sequence:

>121_residues
MDDCFREEEMKLINAIIKPFKLDDVREALADVGIEGMTVSEVKGFGRQKGHTELYRGAEYQVDFLPKVKIEIATQSDNVD
RVVEAIIKAAHTGKIGDGKIFVYDLSQAVRIRTGEMDSEAL

Sequences:

>Translated_121_residues
MDDCFREEEMKLINAIIKPFKLDDVREALADVGIEGMTVSEVKGFGRQKGHTELYRGAEYQVDFLPKVKIEIATQSDNVD
RVVEAIIKAAHTGKIGDGKIFVYDLSQAVRIRTGEMDSEAL
>Mature_121_residues
MDDCFREEEMKLINAIIKPFKLDDVREALADVGIEGMTVSEVKGFGRQKGHTELYRGAEYQVDFLPKVKIEIATQSDNVD
RVVEAIIKAAHTGKIGDGKIFVYDLSQAVRIRTGEMDSEAL

Specific function: P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=75, Blast_Score=171, Evalue=2e-44,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=67.8571428571429, Blast_Score=163, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 13534; Mature: 13534

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDCFREEEMKLINAIIKPFKLDDVREALADVGIEGMTVSEVKGFGRQKGHTELYRGAEY
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHCCCCC
QVDFLPKVKIEIATQSDNVDRVVEAIIKAAHTGKIGDGKIFVYDLSQAVRIRTGEMDSEA
EEECCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHEEECCCCCCCC
L
C
>Mature Secondary Structure
MDDCFREEEMKLINAIIKPFKLDDVREALADVGIEGMTVSEVKGFGRQKGHTELYRGAEY
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHCCCCC
QVDFLPKVKIEIATQSDNVDRVVEAIIKAAHTGKIGDGKIFVYDLSQAVRIRTGEMDSEA
EEECCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHEEECCCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]