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The map label for this gene is fliM [H]

Identifier: 147675314

GI number: 147675314

Start: 1832159

End: 1833208

Strand: Reverse

Name: fliM [H]

Synonym: VC0395_A1709

Alternate gene names: 147675314

Gene position: 1833208-1832159 (Counterclockwise)

Preceding gene: 147673204

Following gene: 147674715

Centisome position: 60.62

GC content: 49.05

Gene sequence:

>1050_bases
GTGACTGATTTATTAAGCCAAGACGAAATCGATGCGCTGCTTCATGGCGTTGACAGCGTCGATGAGGAAGAAGAGGAATT
ACCCAAATCTGGCCCCAGTGCCACGAATTTTGATTTTTCTTCCCAAGATCGTATCGTCCGGGGTCGAATGCCGACTCTGG
AGCTTATCAATGAACGTTTTGCTCGCCATCTGCGCATCAGTCTGTTCAACATGCTGCGTAAAACCGCAGAAGTGTCGATC
AACGGCGTGCAGATGATGAAGTTTGGTGAATACCAAAATACCTTATATGTTCCTACCAGTTTGAACATGGTGCGTTTTCG
TCCACTTAAGGGGACGGCCTTGGTGACCATGGAAGCGCGTTTGGTGTTCATTCTGGTGGAAAACTTCTTCGGCGGCGATG
GCCGTTATCATGCCCGAATTGAGGGACGTGAATTTACCCCAACTGAACGGCGGGTTATTCAACTGCTGCTCAAAATCGTG
TTTGCCGACTACAAAGAAGCGTGGTCGCCCGTCATGGGGGTCGAGTTTGAATACCTCGATTCCGAAGTGAACCCGAGCAT
GGCGAACATCGTCAGCCCAACCGAAGTGATTGTCGTCAGCTCCTTCCACGTTGAAGTGGATGGTGGCGGTGGCGATTTCC
ACATGGTTATGCCGTATTCGATGGTAGAACCGATCCGTGAACTGCTGGATGCGGGTGTACAGTCGGACAAAATGGAAACC
GACGTGCGCTGGAGTACGGCTCTGCGCGAAGAGATCATGGATGTACCAGTCAATTTCCGAGTGAACTTACTGGAAATGGA
TATTGCGCTGCGGGATTTGATGGAGTTGCAAGTGGGGGATGTCATCCCGATAAAAATGCCAGAACATGCGGTAATGTTTG
TCGAAGAGCTGCCAACGTATCGTGTGAAAATGGGACGCTCCGGTGAAAAACTCGCAGTACAAGTTTCTGAGAAAATCAAA
CGCCCAGATGTAGTGAAAACGGATCTCGCGTTCCTTGGTAAAGACATTATTGCTGAGTTTGAAAACGCAGAAGAAGATGA
AATCGAATAA

Upstream 100 bases:

>100_bases
AAAGCCACCGATGACATCAAAGCCGCGCTCAACCAAGCGGTAGGCAAACCCGTTATTGAAAAAGTGCTGTTTACCGACTT
TGTAATGCAATAGGTGAAAT

Downstream 100 bases:

>100_bases
TCCGTTTTACGACGTCGGGATGATCGCTCGATAGCGGCGGATAATGAAAAGAACCAATAGGTAGTTGAAGATGGCAAAAA
GCGAAGATGAAAGACTGGCC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTDLLSQDEIDALLHGVDSVDEEEEELPKSGPSATNFDFSSQDRIVRGRMPTLELINERFARHLRISLFNMLRKTAEVSI
NGVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALVTMEARLVFILVENFFGGDGRYHARIEGREFTPTERRVIQLLLKIV
FADYKEAWSPVMGVEFEYLDSEVNPSMANIVSPTEVIVVSSFHVEVDGGGGDFHMVMPYSMVEPIRELLDAGVQSDKMET
DVRWSTALREEIMDVPVNFRVNLLEMDIALRDLMELQVGDVIPIKMPEHAVMFVEELPTYRVKMGRSGEKLAVQVSEKIK
RPDVVKTDLAFLGKDIIAEFENAEEDEIE

Sequences:

>Translated_349_residues
MTDLLSQDEIDALLHGVDSVDEEEEELPKSGPSATNFDFSSQDRIVRGRMPTLELINERFARHLRISLFNMLRKTAEVSI
NGVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALVTMEARLVFILVENFFGGDGRYHARIEGREFTPTERRVIQLLLKIV
FADYKEAWSPVMGVEFEYLDSEVNPSMANIVSPTEVIVVSSFHVEVDGGGGDFHMVMPYSMVEPIRELLDAGVQSDKMET
DVRWSTALREEIMDVPVNFRVNLLEMDIALRDLMELQVGDVIPIKMPEHAVMFVEELPTYRVKMGRSGEKLAVQVSEKIK
RPDVVKTDLAFLGKDIIAEFENAEEDEIE
>Mature_348_residues
TDLLSQDEIDALLHGVDSVDEEEEELPKSGPSATNFDFSSQDRIVRGRMPTLELINERFARHLRISLFNMLRKTAEVSIN
GVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALVTMEARLVFILVENFFGGDGRYHARIEGREFTPTERRVIQLLLKIVF
ADYKEAWSPVMGVEFEYLDSEVNPSMANIVSPTEVIVVSSFHVEVDGGGGDFHMVMPYSMVEPIRELLDAGVQSDKMETD
VRWSTALREEIMDVPVNFRVNLLEMDIALRDLMELQVGDVIPIKMPEHAVMFVEELPTYRVKMGRSGEKLAVQVSEKIKR
PDVVKTDLAFLGKDIIAEFENAEEDEIE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=319, Percent_Identity=37.9310344827586, Blast_Score=240, Evalue=1e-64,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 39746; Mature: 39615

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLLSQDEIDALLHGVDSVDEEEEELPKSGPSATNFDFSSQDRIVRGRMPTLELINERF
CCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHH
ARHLRISLFNMLRKTAEVSINGVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALVTMEAR
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCEEEEECCCCCEEEEEEHE
LVFILVENFFGGDGRYHARIEGREFTPTERRVIQLLLKIVFADYKEAWSPVMGVEFEYLD
EEEEEEEHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCC
SEVNPSMANIVSPTEVIVVSSFHVEVDGGGGDFHMVMPYSMVEPIRELLDAGVQSDKMET
CCCCCCHHHHCCCCEEEEEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCC
DVRWSTALREEIMDVPVNFRVNLLEMDIALRDLMELQVGDVIPIKMPEHAVMFVEELPTY
CHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCE
RVKMGRSGEKLAVQVSEKIKRPDVVKTDLAFLGKDIIAEFENAEEDEIE
EEECCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TDLLSQDEIDALLHGVDSVDEEEEELPKSGPSATNFDFSSQDRIVRGRMPTLELINERF
CCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHH
ARHLRISLFNMLRKTAEVSINGVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALVTMEAR
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCEEEEECCCCCEEEEEEHE
LVFILVENFFGGDGRYHARIEGREFTPTERRVIQLLLKIVFADYKEAWSPVMGVEFEYLD
EEEEEEEHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCC
SEVNPSMANIVSPTEVIVVSSFHVEVDGGGGDFHMVMPYSMVEPIRELLDAGVQSDKMET
CCCCCCHHHHCCCCEEEEEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCC
DVRWSTALREEIMDVPVNFRVNLLEMDIALRDLMELQVGDVIPIKMPEHAVMFVEELPTY
CHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCE
RVKMGRSGEKLAVQVSEKIKRPDVVKTDLAFLGKDIIAEFENAEEDEIE
EEECCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043; 7622217 [H]