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The map label for this gene is hemN [H]

Identifier: 147675179

GI number: 147675179

Start: 2557785

End: 2559227

Strand: Reverse

Name: hemN [H]

Synonym: VC0395_A2402

Alternate gene names: 147675179

Gene position: 2559227-2557785 (Counterclockwise)

Preceding gene: 147674399

Following gene: 147673490

Centisome position: 84.63

GC content: 48.02

Gene sequence:

>1443_bases
ATGTCGTGTGGCAGAATAGCCCGTCAAGAATTGAGCAAAACGGAAAGATTCATTATGTCGTCGTATGTCGTACCCAGCCA
ACAGATCGTATGGGATCAGGCCATTCTGGATAAGTACAACTACTCCGGTCCGCGTTACACTTCATACCCGACTGCGGTTG
AGTTCCATGAAGCCTTTACCATCGCTGACTTTGATATGGCCTGTACTCAGTACCCAGAGCGACCATTATCGCTGTACATT
CATATCCCGTTTTGCCATAAGCTTTGTTACTACTGTGGTTGTAACAAGGTGATCACTCGTCATTCGCACAAAGCGGATGA
GTACCTTGATGTGCTTGAGCATGAAATTCGTCAACGCGCCTCACTATTAATCGGAAGAAACGTCACTCAACTCCACTTCG
GTGGTGGTACGCCGACGTTCTTGACCAACGCTCAAATCAGTCGTTTGATGACGCTGCTGCGTAGCGAGTTTCACTTCGCC
GATGAGGCGGAAATCAGTATTGAAATCGATCCGCGTGAAATCCAGTTAGAGGTGCTTGATCACCTGCGCCAAGAAGGCTT
TAACCGAGTCAGCATCGGGGTGCAGGATTTCAATAAAGAAGTGCAGAAGCTGGTTAACCGTGAGCAAGATGAGCAGTTCA
TTTTTGATCTGGTTGAACGCGCTAAACAGCTGGGTTTTCGTTCGACCAACCTTGACCTCATTTACGGCTTACCTAAGCAA
ACCGCAGTAACCTTTGCCCAGACGTTGCAACAAGTGCTGACCATGCAACCGGGGCGTTTGTCGGTATTTAACTACGCGCA
CATGCCGCAACTGTTCGCAGCGCAGCGCAAAATTAAAGAAGCCGATTTACCTGCCGCCGAAGAAAAACTGGCGATTTTAC
AGCAGACAATCACCACGCTAACAGGCGCGGGGTATCAGTTCATCGGTATGGATCACTTTGCCTTGCCGGATGATGAGCTA
GCGATTGCTCAGCGCAGTGGTATCTTGCACCGTAACTTCCAAGGCTATACCACCCAAGGTGAATGTGACTTAGTCGGCTT
TGGGGTTTCGGCGATTTCCATGGTGGGCGATGCCTATGCACAAAACCAGAAAGAGCTGAAAAAATACTATGCGCAAGTCA
ATGAGATGCGTCATGCCTTGTGGAAAGGTGTGACACTAGATAAGGATGATTTACTGCGCCGTGAAGTGATTAAGCAGCTG
ATTTGTAATTTCAAGCTTGATAAAACAGCGATTGAGCGTGAGTTTAATCTGCGTTTCAATCACTACTTCCGCCAAGATTT
AGCTCTGCTACAAACCTTTATCGACGATGGGTTGGTGACGGTGGATGAACGCATGATTGAAGTGACGCTGCGTGGGCGTT
TGTTAATTCGCAACATCTGTATGTGTTTTGATAAATACTTGCGTGAGCGGGCGCGTCAGCAGCAGTTCTCGCGAGTCATT
TAA

Upstream 100 bases:

>100_bases
GATTCAGATGGTGTGAAACTAACCCGAAAGGGGGAGTGATCGCGCTAGCAGAATCGAATATTTTGTTGCCAGATCCTGTT
CTGCCGCCCGACGTTCGTCA

Downstream 100 bases:

>100_bases
GACCGCATTCCACAACAAGGCCACCGCATGCGGTGGCCTTGTTGTTTGTGCTCTGATTTTAATTCAGGATGACTGAGCGG
AAGGTGACTCCAGCAAAGAG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSCGRIARQELSKTERFIMSSYVVPSQQIVWDQAILDKYNYSGPRYTSYPTAVEFHEAFTIADFDMACTQYPERPLSLYI
HIPFCHKLCYYCGCNKVITRHSHKADEYLDVLEHEIRQRASLLIGRNVTQLHFGGGTPTFLTNAQISRLMTLLRSEFHFA
DEAEISIEIDPREIQLEVLDHLRQEGFNRVSIGVQDFNKEVQKLVNREQDEQFIFDLVERAKQLGFRSTNLDLIYGLPKQ
TAVTFAQTLQQVLTMQPGRLSVFNYAHMPQLFAAQRKIKEADLPAAEEKLAILQQTITTLTGAGYQFIGMDHFALPDDEL
AIAQRSGILHRNFQGYTTQGECDLVGFGVSAISMVGDAYAQNQKELKKYYAQVNEMRHALWKGVTLDKDDLLRREVIKQL
ICNFKLDKTAIEREFNLRFNHYFRQDLALLQTFIDDGLVTVDERMIEVTLRGRLLIRNICMCFDKYLRERARQQQFSRVI

Sequences:

>Translated_480_residues
MSCGRIARQELSKTERFIMSSYVVPSQQIVWDQAILDKYNYSGPRYTSYPTAVEFHEAFTIADFDMACTQYPERPLSLYI
HIPFCHKLCYYCGCNKVITRHSHKADEYLDVLEHEIRQRASLLIGRNVTQLHFGGGTPTFLTNAQISRLMTLLRSEFHFA
DEAEISIEIDPREIQLEVLDHLRQEGFNRVSIGVQDFNKEVQKLVNREQDEQFIFDLVERAKQLGFRSTNLDLIYGLPKQ
TAVTFAQTLQQVLTMQPGRLSVFNYAHMPQLFAAQRKIKEADLPAAEEKLAILQQTITTLTGAGYQFIGMDHFALPDDEL
AIAQRSGILHRNFQGYTTQGECDLVGFGVSAISMVGDAYAQNQKELKKYYAQVNEMRHALWKGVTLDKDDLLRREVIKQL
ICNFKLDKTAIEREFNLRFNHYFRQDLALLQTFIDDGLVTVDERMIEVTLRGRLLIRNICMCFDKYLRERARQQQFSRVI
>Mature_479_residues
SCGRIARQELSKTERFIMSSYVVPSQQIVWDQAILDKYNYSGPRYTSYPTAVEFHEAFTIADFDMACTQYPERPLSLYIH
IPFCHKLCYYCGCNKVITRHSHKADEYLDVLEHEIRQRASLLIGRNVTQLHFGGGTPTFLTNAQISRLMTLLRSEFHFAD
EAEISIEIDPREIQLEVLDHLRQEGFNRVSIGVQDFNKEVQKLVNREQDEQFIFDLVERAKQLGFRSTNLDLIYGLPKQT
AVTFAQTLQQVLTMQPGRLSVFNYAHMPQLFAAQRKIKEADLPAAEEKLAILQQTITTLTGAGYQFIGMDHFALPDDELA
IAQRSGILHRNFQGYTTQGECDLVGFGVSAISMVGDAYAQNQKELKKYYAQVNEMRHALWKGVTLDKDDLLRREVIKQLI
CNFKLDKTAIEREFNLRFNHYFRQDLALLQTFIDDGLVTVDERMIEVTLRGRLLIRNICMCFDKYLRERARQQQFSRVI

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=246, Percent_Identity=27.2357723577236, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI87082341, Length=454, Percent_Identity=69.1629955947137, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1789325, Length=243, Percent_Identity=32.9218106995885, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 55618; Mature: 55486

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCGRIARQELSKTERFIMSSYVVPSQQIVWDQAILDKYNYSGPRYTSYPTAVEFHEAFT
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEE
IADFDMACTQYPERPLSLYIHIPFCHKLCYYCGCNKVITRHSHKADEYLDVLEHEIRQRA
EECHHHHHHHCCCCCEEEEEECHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHH
SLLIGRNVTQLHFGGGTPTFLTNAQISRLMTLLRSEFHFADEAEISIEIDPREIQLEVLD
HHHHCCCCEEEEECCCCCCEECHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHH
HLRQEGFNRVSIGVQDFNKEVQKLVNREQDEQFIFDLVERAKQLGFRSTNLDLIYGLPKQ
HHHHCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHH
TAVTFAQTLQQVLTMQPGRLSVFNYAHMPQLFAAQRKIKEADLPAAEEKLAILQQTITTL
HHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TGAGYQFIGMDHFALPDDELAIAQRSGILHRNFQGYTTQGECDLVGFGVSAISMVGDAYA
HCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
QNQKELKKYYAQVNEMRHALWKGVTLDKDDLLRREVIKQLICNFKLDKTAIEREFNLRFN
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
HYFRQDLALLQTFIDDGLVTVDERMIEVTLRGRLLIRNICMCFDKYLRERARQQQFSRVI
HHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SCGRIARQELSKTERFIMSSYVVPSQQIVWDQAILDKYNYSGPRYTSYPTAVEFHEAFT
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEE
IADFDMACTQYPERPLSLYIHIPFCHKLCYYCGCNKVITRHSHKADEYLDVLEHEIRQRA
EECHHHHHHHCCCCCEEEEEECHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHH
SLLIGRNVTQLHFGGGTPTFLTNAQISRLMTLLRSEFHFADEAEISIEIDPREIQLEVLD
HHHHCCCCEEEEECCCCCCEECHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHH
HLRQEGFNRVSIGVQDFNKEVQKLVNREQDEQFIFDLVERAKQLGFRSTNLDLIYGLPKQ
HHHHCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHH
TAVTFAQTLQQVLTMQPGRLSVFNYAHMPQLFAAQRKIKEADLPAAEEKLAILQQTITTL
HHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TGAGYQFIGMDHFALPDDELAIAQRSGILHRNFQGYTTQGECDLVGFGVSAISMVGDAYA
HCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
QNQKELKKYYAQVNEMRHALWKGVTLDKDDLLRREVIKQLICNFKLDKTAIEREFNLRFN
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
HYFRQDLALLQTFIDDGLVTVDERMIEVTLRGRLLIRNICMCFDKYLRERARQQQFSRVI
HHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7768836; 8346018; 9278503 [H]