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The map label for this gene is gshA

Identifier: 147675148

GI number: 147675148

Start: 80869

End: 82443

Strand: Direct

Name: gshA

Synonym: VC0395_A0090

Alternate gene names: 147675148

Gene position: 80869-82443 (Clockwise)

Preceding gene: 147673676

Following gene: 147674316

Centisome position: 2.67

GC content: 50.29

Gene sequence:

>1575_bases
TTGAGTAAATTTGCCGAGCGACTACAAACAGTTGCAAACAAACCAGAAGTATTTCAAAAGTTTAGCCGTGGATTGGAGCG
CGAATCGCTGCGTTACACGCCGGAAGGTGCTCTCACGCAAACGCCACACCCGAAAGCACTGGGAGCGGCATTAACTCACC
GTTGGATCACCACAGATTTTGCGGAGTCCTTGCTGGAGTTCATCACCCCTGTCTCGCATGAAGTCGATACTCTGCTGGCG
CAGATGTCGGATATTCACCATTTCGCGCAGACCAAGCTGGGCGATGAAAAACTTTGGCCTCTCTCCATGCCTTGCTACGT
TGGCAGTGAAGAGGGGATAGTGCTGGCACAATACGGTTCGTCGAACACTGGAAAAATGAAAACCTTGTACCGTGAAGGGC
TCAAGCGTCGTTATGGCAGCCTGATGCAGATTATTTCCGGAGTACATTTCAATTTTTCATTCCCAGAAAGTTTCTGGGAT
GCCTTGTTTGGTGAGCAAGAAGAGTCTGCTCGTCAAGCGAGTAAATCTGCGGCCTATTTTGGTGTCATTCGTAATTACTA
CCGTTTTGGTTGGCTGATCCCGTATCTGTTTGGCGCTTCCCCCGCACTCTGCTCATCTTTTTTACAAGGTCGCAAAACCG
ATCTGCCGTTCGAGTCCATTGGTAAAACCTTGTATCTGCCTTATGCCACCTCGCTGCGTCTAAGCGATTTGGGCTACACC
AACAGCGCGCAAAGTGTGCTGCAAATTGGTTTCAATAGCATAGAGCAGTATCTGCAAGGGTTGAATGAAGCGATCCGCAC
CCCCTCAGCCGAGTTTGCCAAACTGGGTGTGAAAGTAGAGGGAGAATATCGCCAGCTCAATAGCAACGTACTGCAAATTG
AAAATGAGCTGTATGCGCCGATTCGTCCGAAACGTGTGACACAAAGTGGCGAGCGGCCTTCGCAAGCCCTAGCGCGTGCA
GGGGTGGAATATATTGAAGTGCGCTCGCTCGATGTGAACCCATTTAGCCCGATTGGAATTAATGAAGATCAGGTTCGTTT
CCTCGACCTATTCTTAGCATGGACAGCGCTGTCAGATTCTGATCCTATGGATGGCTGTGAACTGGCTTGTTGGCGCGACA
ACTGGCGTAAAGTGGTACTGGAAGGTCGCAAGCCTGGCTTAGAACTGCAAATTGGCTGCCATGGTGAAGTGTTGACCTTG
CAAGCGTGGGCGAAGCGTGTGTTTGCCGATCTGCGTGTGATTGCTGAAACTATGGATGCGGCTCTCGGCGGCGATGCCTA
CCAAGCGGTGTGTGCCAAACTAGAGACTTGGATCGAAAATCCTGAGCTGACGCTCTCGGCACAAATCCTAGCAGAAATTA
AACAGCACGGCGGCTTAGGTAAAACAGGCTGCGCGTTAGGTAACGAGTACCGAGCGGTCAATTTACAGCACCAATACCAG
TTCTACTCACAAGATGAGATGGAGCAGGAAGTGCGCCGCTCAACTCAAGCCCAAGCCGACATTGAAGCGAAAGAGACGCT
CAATTTTGATGCTTACCTAACGCAATATTTTGCCTACCTACAGGCGGTGAGTTAA

Upstream 100 bases:

>100_bases
AATAGTGGGGCGGATTGCGCCCCAAACTCGCTATGCTGCTTAAGCACTACGCTTAACAGGCGGCGTGCGACTGGAACCAC
CTACATAAGCGAAGCGTATT

Downstream 100 bases:

>100_bases
TTTAGTTTTAGTGGTGCGCAGTAAGCGAGCCAGAAAGGGAGAAAGGAAATGCCATTATTAGACAGTTTTACCGTCGATCA
TACTCGTATGAATGCACCGG

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSKFAERLQTVANKPEVFQKFSRGLERESLRYTPEGALTQTPHPKALGAALTHRWITTDFAESLLEFITPVSHEVDTLLA
QMSDIHHFAQTKLGDEKLWPLSMPCYVGSEEGIVLAQYGSSNTGKMKTLYREGLKRRYGSLMQIISGVHFNFSFPESFWD
ALFGEQEESARQASKSAAYFGVIRNYYRFGWLIPYLFGASPALCSSFLQGRKTDLPFESIGKTLYLPYATSLRLSDLGYT
NSAQSVLQIGFNSIEQYLQGLNEAIRTPSAEFAKLGVKVEGEYRQLNSNVLQIENELYAPIRPKRVTQSGERPSQALARA
GVEYIEVRSLDVNPFSPIGINEDQVRFLDLFLAWTALSDSDPMDGCELACWRDNWRKVVLEGRKPGLELQIGCHGEVLTL
QAWAKRVFADLRVIAETMDAALGGDAYQAVCAKLETWIENPELTLSAQILAEIKQHGGLGKTGCALGNEYRAVNLQHQYQ
FYSQDEMEQEVRRSTQAQADIEAKETLNFDAYLTQYFAYLQAVS

Sequences:

>Translated_524_residues
MSKFAERLQTVANKPEVFQKFSRGLERESLRYTPEGALTQTPHPKALGAALTHRWITTDFAESLLEFITPVSHEVDTLLA
QMSDIHHFAQTKLGDEKLWPLSMPCYVGSEEGIVLAQYGSSNTGKMKTLYREGLKRRYGSLMQIISGVHFNFSFPESFWD
ALFGEQEESARQASKSAAYFGVIRNYYRFGWLIPYLFGASPALCSSFLQGRKTDLPFESIGKTLYLPYATSLRLSDLGYT
NSAQSVLQIGFNSIEQYLQGLNEAIRTPSAEFAKLGVKVEGEYRQLNSNVLQIENELYAPIRPKRVTQSGERPSQALARA
GVEYIEVRSLDVNPFSPIGINEDQVRFLDLFLAWTALSDSDPMDGCELACWRDNWRKVVLEGRKPGLELQIGCHGEVLTL
QAWAKRVFADLRVIAETMDAALGGDAYQAVCAKLETWIENPELTLSAQILAEIKQHGGLGKTGCALGNEYRAVNLQHQYQ
FYSQDEMEQEVRRSTQAQADIEAKETLNFDAYLTQYFAYLQAVS
>Mature_523_residues
SKFAERLQTVANKPEVFQKFSRGLERESLRYTPEGALTQTPHPKALGAALTHRWITTDFAESLLEFITPVSHEVDTLLAQ
MSDIHHFAQTKLGDEKLWPLSMPCYVGSEEGIVLAQYGSSNTGKMKTLYREGLKRRYGSLMQIISGVHFNFSFPESFWDA
LFGEQEESARQASKSAAYFGVIRNYYRFGWLIPYLFGASPALCSSFLQGRKTDLPFESIGKTLYLPYATSLRLSDLGYTN
SAQSVLQIGFNSIEQYLQGLNEAIRTPSAEFAKLGVKVEGEYRQLNSNVLQIENELYAPIRPKRVTQSGERPSQALARAG
VEYIEVRSLDVNPFSPIGINEDQVRFLDLFLAWTALSDSDPMDGCELACWRDNWRKVVLEGRKPGLELQIGCHGEVLTLQ
AWAKRVFADLRVIAETMDAALGGDAYQAVCAKLETWIENPELTLSAQILAEIKQHGGLGKTGCALGNEYRAVNLQHQYQF
YSQDEMEQEVRRSTQAQADIEAKETLNFDAYLTQYFAYLQAVS

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789044, Length=517, Percent_Identity=54.7388781431335, Blast_Score=587, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSH1_VIBCH (Q9KUG5)

Other databases:

- EMBL:   AE003852
- PIR:   E82309
- RefSeq:   NP_230207.1
- ProteinModelPortal:   Q9KUG5
- SMR:   Q9KUG5
- GeneID:   2615233
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0556
- TIGR:   VC_0556
- HOGENOM:   HBG289023
- OMA:   FGLIRNY
- ProtClustDB:   PRK02107
- BRENDA:   6.3.2.2
- HAMAP:   MF_00578
- InterPro:   IPR007370
- InterPro:   IPR006334
- TIGRFAMs:   TIGR01434

Pfam domain/function: PF04262 Glu_cys_ligase

EC number: =6.3.2.2

Molecular weight: Translated: 58961; Mature: 58830

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFAERLQTVANKPEVFQKFSRGLERESLRYTPEGALTQTPHPKALGAALTHRWITTDF
CCHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AESLLEFITPVSHEVDTLLAQMSDIHHFAQTKLGDEKLWPLSMPCYVGSEEGIVLAQYGS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEEECCC
SNTGKMKTLYREGLKRRYGSLMQIISGVHFNFSFPESFWDALFGEQEESARQASKSAAYF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
GVIRNYYRFGWLIPYLFGASPALCSSFLQGRKTDLPFESIGKTLYLPYATSLRLSDLGYT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCEEEECCCCCEEECCCCCC
NSAQSVLQIGFNSIEQYLQGLNEAIRTPSAEFAKLGVKVEGEYRQLNSNVLQIENELYAP
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEECCHHHHHCCCCEEECHHHCCC
IRPKRVTQSGERPSQALARAGVEYIEVRSLDVNPFSPIGINEDQVRFLDLFLAWTALSDS
CCCHHHCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
DPMDGCELACWRDNWRKVVLEGRKPGLELQIGCHGEVLTLQAWAKRVFADLRVIAETMDA
CCCCCCEEEEEHHCCHHEEECCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHH
ALGGDAYQAVCAKLETWIENPELTLSAQILAEIKQHGGLGKTGCALGNEYRAVNLQHQYQ
HHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEHHHHH
FYSQDEMEQEVRRSTQAQADIEAKETLNFDAYLTQYFAYLQAVS
HCCHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKFAERLQTVANKPEVFQKFSRGLERESLRYTPEGALTQTPHPKALGAALTHRWITTDF
CHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AESLLEFITPVSHEVDTLLAQMSDIHHFAQTKLGDEKLWPLSMPCYVGSEEGIVLAQYGS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEEECCC
SNTGKMKTLYREGLKRRYGSLMQIISGVHFNFSFPESFWDALFGEQEESARQASKSAAYF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
GVIRNYYRFGWLIPYLFGASPALCSSFLQGRKTDLPFESIGKTLYLPYATSLRLSDLGYT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCEEEECCCCCEEECCCCCC
NSAQSVLQIGFNSIEQYLQGLNEAIRTPSAEFAKLGVKVEGEYRQLNSNVLQIENELYAP
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEECCHHHHHCCCCEEECHHHCCC
IRPKRVTQSGERPSQALARAGVEYIEVRSLDVNPFSPIGINEDQVRFLDLFLAWTALSDS
CCCHHHCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
DPMDGCELACWRDNWRKVVLEGRKPGLELQIGCHGEVLTLQAWAKRVFADLRVIAETMDA
CCCCCCEEEEEHHCCHHEEECCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHH
ALGGDAYQAVCAKLETWIENPELTLSAQILAEIKQHGGLGKTGCALGNEYRAVNLQHQYQ
HHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEHHHHH
FYSQDEMEQEVRRSTQAQADIEAKETLNFDAYLTQYFAYLQAVS
HCCHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301