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The map label for this gene is imp [H]

Identifier: 147675099

GI number: 147675099

Start: 3016952

End: 3019315

Strand: Reverse

Name: imp [H]

Synonym: VC0395_A2864

Alternate gene names: 147675099

Gene position: 3019315-3016952 (Counterclockwise)

Preceding gene: 147673592

Following gene: 147674503

Centisome position: 99.84

GC content: 46.7

Gene sequence:

>2364_bases
ATGTACAACCCCATGTCATGCTTTTCCCGCACGTTTTTAGCCGCTTCGATTAGCGCTGCACTCTTTGCACCTCAGATACA
AGCAGAAGCAAGTGTGGATGACAATCGCGCTCAATTACCTAATGGTGAGCAGTGTTTAGTCAACCAGCCTGAGCCTACTA
ATCCCGGCCAACAGCCTATCAATGTCGAAGCGGATAAGCTAGAGGCAATCAATGGGCAGAAGGCGACCTATTCGGGGAAT
GTCGTTGTCGTGCAAGGTAAGAAACGCATTGCCGCGGATAACGTGACTTTGCATCAGCAAGAGAATGTGGTTGTCGCTGA
AGGTAATGTTCAGTTCAGTGATGGTGAGATAAAAACACACTCCACCAAAGCGACCAACCATCTCAATACCGATGAAATGA
CGCTGGAAAATACTCGCTACCAGTTTTTGTGTGAACCGGGCCGTGGTGAAGCGGTGTATGTTTCGAAAACGGGCAAAGCC
GTGTATGAAATTGAAGATGGCTCCATCACCTCATGTCCTGATGGTGATAATGCATGGCGGATGCGTGCTTCCAGCATTGA
TGTGGATCAGAATGAAGAGATTGCGACCTTCTACAATCCGCGTCTTGAAGTGCAAAACGTACCTGTGTTCTATCTCCCCT
ATTTGACTGTGCCAATCGGTGATACGCGTAAAACTGGTTTTCTATACCCGACGGCCTCTTACGGCTCACGAAATGGCTAT
TCGTTTGAAGTGCCAATTTATTGGAACTTGGCACCCCAATATGACTTAGAAACCACCTTTAACTATATGCAAAAGCGCGG
AACACAACTGAATAGTGTGTTCCGTTATCTGACCGATTTTGGTGCGGGTCAAATCAAATCTGAATATCTTGCCGACGATC
AGCTGCACACTGAATTAGGCGATCGCTGGGCATTCCAATATGAGCATAACGGCATTTTCCAACAAGCTTGGAAATTTGAA
ATCGACTACTCCAAAGTCAGCGATATCAACTATTTCTCTGACTTAGATTCGGGAGTCGGTAATCGTGAAGATGGACAACT
CATCCAAGAAGGGCGTGCCACTTACCGTAGCGACAACTGGGATTCTGCGCTGTTGGTGCGGGATTTCCAACTACTGACCA
AGGATACCACTAGCACGAACTTGCCTTATCGGTTAATGCCCCAGCTCAGCTACAACTACTATGCTCCAGAGACGATGAAG
TATCTGGATCTTGACCTTGTCAGCCATGTCTCGCGCTTTGAAACCGATGCAAGAGGCAAACCCTCTGCAACTCGAGTACA
TATTGAACCTGGGCTCAAAATTCCGTTCAGCAACACTTGGGGCAACTGGACAACCGAAGCACGTGTCTTGGGCACCTACT
ATCAACAAGATCTTGATAAGACCACAGATGCCAAACTGGAAGAGAGTGTGACACGCGTTATCCCTGAGATTCGCAGTGTA
GCGGGCATTGTGCTTGAACGAGATACGGTACTGCTTGATGACTACACTCAAACCCTTGAACCCAAAATTCAATATCTCTA
CGTGCCAGAAAAATATCAGGACAATATTGGTCTGTATGATTCCACTTTGCTGCAAACCGACTACTACGGGCTATTCCGCA
GTCGCAAATACAGCGGCGTAGACCGAATTGAGTCCGCCAACCAAGTCAGCTACGGTGCATCGACTCGTTTCTTTGATAGC
AACTATAAAGAGCGCCTCAATATCGCGTTTGGTCAGATTTTCTATCTGGACAGCAAGCTCAATCCGTCCAATAAAAATCC
AGACAGCACATCGGATAAAACCAGCTATTCCGCTTGGGCCGTTGAGATGGACTTTAACTTTGCTGACTATCTGTTCTATC
ACGGCGGCATTCAATACGACATCGATAGCCAAGCCGTTCAACTTGGCAACAGTACATTAGAATATCGAGTCGCCAGCGGT
TATATTCAAGCTAACTACCGCTATGTTGCTAAGGATTATATTCGCAATACCGTCGGTGACAGCATTACCAATATTGATGA
CATTACCCGAGACGGTATTTCACAAGCGGGCATTTTGGCGGGTTACCAGTTGAGTCGTAAATGGTCGGCTAGCGGTCAAT
ATTACTATGACCTGACCACAGACGAGGCGTTAGAGTGGCTGGCTAACCTGACTTACACTTCAGATTGTTGGTATGTGGGC
TTTACTTACAGTAACCAATTAAAAAGCTGGAATGGCAACTTTGTTACGGATCCTTACGCGACTCCGATTTATGAGAATAA
CTTCAGCTTTAACATCGGGATTATTGGTTTTGGCACCAGCATTGGCGCAGGCTCAAGCATGACAGGTGTCGATAGCGCAG
GTAACTCACTCGGTTATGGTCGCCCATTCTTCCTGAATAACTAA

Upstream 100 bases:

>100_bases
AGTGGCGCAAGCGTCAATTTCCTTTATGATAAGGACCGTTTATATTTCAGGCTCCTAAGTTCGGAATCCATCAAGTTCAA
CAGGCCACAAGTTCAACAGG

Downstream 100 bases:

>100_bases
TTCACCTAGCCTGAGGAAACTCAGGCTTAAAACATTTTAGATAGGACTGCACATGAAATTGTGGAAACCCACACTAATTA
GCGTTCTCAGTGCGCTGACT

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 787; Mature: 787

Protein sequence:

>787_residues
MYNPMSCFSRTFLAASISAALFAPQIQAEASVDDNRAQLPNGEQCLVNQPEPTNPGQQPINVEADKLEAINGQKATYSGN
VVVVQGKKRIAADNVTLHQQENVVVAEGNVQFSDGEIKTHSTKATNHLNTDEMTLENTRYQFLCEPGRGEAVYVSKTGKA
VYEIEDGSITSCPDGDNAWRMRASSIDVDQNEEIATFYNPRLEVQNVPVFYLPYLTVPIGDTRKTGFLYPTASYGSRNGY
SFEVPIYWNLAPQYDLETTFNYMQKRGTQLNSVFRYLTDFGAGQIKSEYLADDQLHTELGDRWAFQYEHNGIFQQAWKFE
IDYSKVSDINYFSDLDSGVGNREDGQLIQEGRATYRSDNWDSALLVRDFQLLTKDTTSTNLPYRLMPQLSYNYYAPETMK
YLDLDLVSHVSRFETDARGKPSATRVHIEPGLKIPFSNTWGNWTTEARVLGTYYQQDLDKTTDAKLEESVTRVIPEIRSV
AGIVLERDTVLLDDYTQTLEPKIQYLYVPEKYQDNIGLYDSTLLQTDYYGLFRSRKYSGVDRIESANQVSYGASTRFFDS
NYKERLNIAFGQIFYLDSKLNPSNKNPDSTSDKTSYSAWAVEMDFNFADYLFYHGGIQYDIDSQAVQLGNSTLEYRVASG
YIQANYRYVAKDYIRNTVGDSITNIDDITRDGISQAGILAGYQLSRKWSASGQYYYDLTTDEALEWLANLTYTSDCWYVG
FTYSNQLKSWNGNFVTDPYATPIYENNFSFNIGIIGFGTSIGAGSSMTGVDSAGNSLGYGRPFFLNN

Sequences:

>Translated_787_residues
MYNPMSCFSRTFLAASISAALFAPQIQAEASVDDNRAQLPNGEQCLVNQPEPTNPGQQPINVEADKLEAINGQKATYSGN
VVVVQGKKRIAADNVTLHQQENVVVAEGNVQFSDGEIKTHSTKATNHLNTDEMTLENTRYQFLCEPGRGEAVYVSKTGKA
VYEIEDGSITSCPDGDNAWRMRASSIDVDQNEEIATFYNPRLEVQNVPVFYLPYLTVPIGDTRKTGFLYPTASYGSRNGY
SFEVPIYWNLAPQYDLETTFNYMQKRGTQLNSVFRYLTDFGAGQIKSEYLADDQLHTELGDRWAFQYEHNGIFQQAWKFE
IDYSKVSDINYFSDLDSGVGNREDGQLIQEGRATYRSDNWDSALLVRDFQLLTKDTTSTNLPYRLMPQLSYNYYAPETMK
YLDLDLVSHVSRFETDARGKPSATRVHIEPGLKIPFSNTWGNWTTEARVLGTYYQQDLDKTTDAKLEESVTRVIPEIRSV
AGIVLERDTVLLDDYTQTLEPKIQYLYVPEKYQDNIGLYDSTLLQTDYYGLFRSRKYSGVDRIESANQVSYGASTRFFDS
NYKERLNIAFGQIFYLDSKLNPSNKNPDSTSDKTSYSAWAVEMDFNFADYLFYHGGIQYDIDSQAVQLGNSTLEYRVASG
YIQANYRYVAKDYIRNTVGDSITNIDDITRDGISQAGILAGYQLSRKWSASGQYYYDLTTDEALEWLANLTYTSDCWYVG
FTYSNQLKSWNGNFVTDPYATPIYENNFSFNIGIIGFGTSIGAGSSMTGVDSAGNSLGYGRPFFLNN
>Mature_787_residues
MYNPMSCFSRTFLAASISAALFAPQIQAEASVDDNRAQLPNGEQCLVNQPEPTNPGQQPINVEADKLEAINGQKATYSGN
VVVVQGKKRIAADNVTLHQQENVVVAEGNVQFSDGEIKTHSTKATNHLNTDEMTLENTRYQFLCEPGRGEAVYVSKTGKA
VYEIEDGSITSCPDGDNAWRMRASSIDVDQNEEIATFYNPRLEVQNVPVFYLPYLTVPIGDTRKTGFLYPTASYGSRNGY
SFEVPIYWNLAPQYDLETTFNYMQKRGTQLNSVFRYLTDFGAGQIKSEYLADDQLHTELGDRWAFQYEHNGIFQQAWKFE
IDYSKVSDINYFSDLDSGVGNREDGQLIQEGRATYRSDNWDSALLVRDFQLLTKDTTSTNLPYRLMPQLSYNYYAPETMK
YLDLDLVSHVSRFETDARGKPSATRVHIEPGLKIPFSNTWGNWTTEARVLGTYYQQDLDKTTDAKLEESVTRVIPEIRSV
AGIVLERDTVLLDDYTQTLEPKIQYLYVPEKYQDNIGLYDSTLLQTDYYGLFRSRKYSGVDRIESANQVSYGASTRFFDS
NYKERLNIAFGQIFYLDSKLNPSNKNPDSTSDKTSYSAWAVEMDFNFADYLFYHGGIQYDIDSQAVQLGNSTLEYRVASG
YIQANYRYVAKDYIRNTVGDSITNIDDITRDGISQAGILAGYQLSRKWSASGQYYYDLTTDEALEWLANLTYTSDCWYVG
FTYSNQLKSWNGNFVTDPYATPIYENNFSFNIGIIGFGTSIGAGSSMTGVDSAGNSLGYGRPFFLNN

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=783, Percent_Identity=35.8876117496807, Blast_Score=476, Evalue=1e-135,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 89018; Mature: 89018

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNPMSCFSRTFLAASISAALFAPQIQAEASVDDNRAQLPNGEQCLVNQPEPTNPGQQPI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
NVEADKLEAINGQKATYSGNVVVVQGKKRIAADNVTLHQQENVVVAEGNVQFSDGEIKTH
CCCHHHHHCCCCCCEEECCCEEEEECCCEEEECCEEEEECCCEEEECCCEEECCCCEEEC
STKATNHLNTDEMTLENTRYQFLCEPGRGEAVYVSKTGKAVYEIEDGSITSCPDGDNAWR
CCCCCCCCCCCCEEECCCCEEEEEECCCCCEEEEEECCCEEEEECCCCEECCCCCCCCEE
MRASSIDVDQNEEIATFYNPRLEVQNVPVFYLPYLTVPIGDTRKTGFLYPTASYGSRNGY
EEECCCCCCCCCCEEEEECCEEEEECCCEEEEEEEEEECCCCCCCEEEEECCCCCCCCCE
SFEVPIYWNLAPQYDLETTFNYMQKRGTQLNSVFRYLTDFGAGQIKSEYLADDQLHTELG
EEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHC
DRWAFQYEHNGIFQQAWKFEIDYSKVSDINYFSDLDSGVGNREDGQLIQEGRATYRSDNW
CEEEEEEECCCCCHHHEEEEECHHHCCCCHHHHHHHHCCCCCCCCHHHHHCCHHCCCCCC
DSALLVRDFQLLTKDTTSTNLPYRLMPQLSYNYYAPETMKYLDLDLVSHVSRFETDARGK
CCEEEEEHHHHHCCCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCC
PSATRVHIEPGLKIPFSNTWGNWTTEARVLGTYYQQDLDKTTDAKLEESVTRVIPEIRSV
CCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AGIVLERDTVLLDDYTQTLEPKIQYLYVPEKYQDNIGLYDSTLLQTDYYGLFRSRKYSGV
HHEEEECCEEEECCCHHCCCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCH
DRIESANQVSYGASTRFFDSNYKERLNIAFGQIFYLDSKLNPSNKNPDSTSDKTSYSAWA
HHHCCCCCCCCCCCEEEECCCCHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEE
VEMDFNFADYLFYHGGIQYDIDSQAVQLGNSTLEYRVASGYIQANYRYVAKDYIRNTVGD
EEECCCHHHHHEECCCEEECCCHHHHHCCCCEEEEEEECCEEEECEEEEHHHHHHHHHCC
SITNIDDITRDGISQAGILAGYQLSRKWSASGQYYYDLTTDEALEWLANLTYTSDCWYVG
CCCCHHHHHHHCCCCCCEEECEEECCEECCCCCEEEECCHHHHHHHHHCCEEECCEEEEE
FTYSNQLKSWNGNFVTDPYATPIYENNFSFNIGIIGFGTSIGAGSSMTGVDSAGNSLGYG
EEECCCCCCCCCCEEECCCCCCEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
RPFFLNN
CCEEECC
>Mature Secondary Structure
MYNPMSCFSRTFLAASISAALFAPQIQAEASVDDNRAQLPNGEQCLVNQPEPTNPGQQPI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
NVEADKLEAINGQKATYSGNVVVVQGKKRIAADNVTLHQQENVVVAEGNVQFSDGEIKTH
CCCHHHHHCCCCCCEEECCCEEEEECCCEEEECCEEEEECCCEEEECCCEEECCCCEEEC
STKATNHLNTDEMTLENTRYQFLCEPGRGEAVYVSKTGKAVYEIEDGSITSCPDGDNAWR
CCCCCCCCCCCCEEECCCCEEEEEECCCCCEEEEEECCCEEEEECCCCEECCCCCCCCEE
MRASSIDVDQNEEIATFYNPRLEVQNVPVFYLPYLTVPIGDTRKTGFLYPTASYGSRNGY
EEECCCCCCCCCCEEEEECCEEEEECCCEEEEEEEEEECCCCCCCEEEEECCCCCCCCCE
SFEVPIYWNLAPQYDLETTFNYMQKRGTQLNSVFRYLTDFGAGQIKSEYLADDQLHTELG
EEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHC
DRWAFQYEHNGIFQQAWKFEIDYSKVSDINYFSDLDSGVGNREDGQLIQEGRATYRSDNW
CEEEEEEECCCCCHHHEEEEECHHHCCCCHHHHHHHHCCCCCCCCHHHHHCCHHCCCCCC
DSALLVRDFQLLTKDTTSTNLPYRLMPQLSYNYYAPETMKYLDLDLVSHVSRFETDARGK
CCEEEEEHHHHHCCCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCC
PSATRVHIEPGLKIPFSNTWGNWTTEARVLGTYYQQDLDKTTDAKLEESVTRVIPEIRSV
CCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AGIVLERDTVLLDDYTQTLEPKIQYLYVPEKYQDNIGLYDSTLLQTDYYGLFRSRKYSGV
HHEEEECCEEEECCCHHCCCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCH
DRIESANQVSYGASTRFFDSNYKERLNIAFGQIFYLDSKLNPSNKNPDSTSDKTSYSAWA
HHHCCCCCCCCCCCEEEECCCCHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEE
VEMDFNFADYLFYHGGIQYDIDSQAVQLGNSTLEYRVASGYIQANYRYVAKDYIRNTVGD
EEECCCHHHHHEECCCEEECCCHHHHHCCCCEEEEEEECCEEEECEEEEHHHHHHHHHCC
SITNIDDITRDGISQAGILAGYQLSRKWSASGQYYYDLTTDEALEWLANLTYTSDCWYVG
CCCCHHHHHHHCCCCCCEEECEEECCEECCCCCEEEECCHHHHHHHHHCCEEECCEEEEE
FTYSNQLKSWNGNFVTDPYATPIYENNFSFNIGIIGFGTSIGAGSSMTGVDSAGNSLGYG
EEECCCCCCCCCCEEECCCCCCEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
RPFFLNN
CCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]