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The map label for this gene is cheB-1 [H]

Identifier: 147675091

GI number: 147675091

Start: 1049678

End: 1050754

Strand: Reverse

Name: cheB-1 [H]

Synonym: VC0395_A1013

Alternate gene names: 147675091

Gene position: 1050754-1049678 (Counterclockwise)

Preceding gene: 147674323

Following gene: 147675588

Centisome position: 34.75

GC content: 48.38

Gene sequence:

>1077_bases
ATGAAGATTTTAGTTGTCGATGATTCTGCGTTAATGCGCACCACCATTAGCGACATATTGCAAAACATTCCGAATGCCCA
GATTAAAACAGCACGTGATGGTATGGATGCCATCGACAAAGTGATGAAATGGCAACCGGATGTGATGACGTTAGACATCA
ATATGCCCAACATGGATGGTCTAACTTGCCTTACGCAAATCATGGTTGAACGTCCCTTGCCAATTGTCATGCTCTCATCC
CTCACCCATGAAGGTGCCATTACAACGCTCGAAGCGCTTTATCTTGGTGCGGTAGACTTTGTCGCCAAACCCGGAGGCAC
TATCTGCGGTCGATTACAAGAAGTCGCACCATTGATCCGTGAAAAAGTTCGCGCGGCAGCGAAAATGAGAGTCAAAGTTC
ACACGCAGCTTTCGGTATCACCAGCAGAAGTAGAGCACAAACATAAGTCACCTGTGGTGGAAAATAATGAAGACCACCAG
CTTGCGATCATGGGGGTTTCGACAGGAGGGCCCGGCACTGTTGAAACGATACTGCGCCATCTGCCCGCCGATTTCTCATT
ACCTATTATTGTTAACCAACACATGCCAGAGAGCTTCACTGCCGCCTTTGCACAAAGGCTTAATCGCCATCTGCAACAAC
CCGTGAAAGAGATTAACCGTGCTTTAGTGCTTGAGGCTGGTACCGTTTATGTTTGTAAGGGTGACCGAGACTGCATTGTC
ACGCTACGTGATGGTAAATTGGCTGCCATGCCCACGCCAAATGATAGCCATTTTTCATGGCATCCCAGCGTCTCCAAACT
GGTTGCCAGCGCCATACGCACTTGTGGAGAAGATAACTTATTGTGCGTCATGCTAACGGGAATGGGCGACGACGGTGCCA
ATGAAATGGCTCAGGTTGCTCAAGGAAACGGTATTGTTTTTGCCCAAGAACCCACAACCTGCGTAGTCCCCAGTATGCCG
GAAGCATTACTTAAACGTGTACCCCATATTCCAACAGGAACTCCAGAGCACTTAGCTTATCTGATGACCGAAGTGGTGAG
ACAGTCCAGTAGGGAGCTTCACTATGGCAATCATTAA

Upstream 100 bases:

>100_bases
GAGTACTGTCCGCCGATCAATTGATCAGTAATACCGAGATGTACAAGTTACACATGGCTGCAAACGATATGGATAGCTAA
GTTTTAAGGCTGGAGGGAAC

Downstream 100 bases:

>100_bases
ACGCAGCGCTGCTACAGACAACCCAAATATTGAGATACCTTCTACTCCCGAGCAGATCGATGTGCTGAGCGCAGAAGCGC
TGATGGAAGCTCTACAAGGT

Product: protein-glutamate methylesterase CheB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKILVVDDSALMRTTISDILQNIPNAQIKTARDGMDAIDKVMKWQPDVMTLDINMPNMDGLTCLTQIMVERPLPIVMLSS
LTHEGAITTLEALYLGAVDFVAKPGGTICGRLQEVAPLIREKVRAAAKMRVKVHTQLSVSPAEVEHKHKSPVVENNEDHQ
LAIMGVSTGGPGTVETILRHLPADFSLPIIVNQHMPESFTAAFAQRLNRHLQQPVKEINRALVLEAGTVYVCKGDRDCIV
TLRDGKLAAMPTPNDSHFSWHPSVSKLVASAIRTCGEDNLLCVMLTGMGDDGANEMAQVAQGNGIVFAQEPTTCVVPSMP
EALLKRVPHIPTGTPEHLAYLMTEVVRQSSRELHYGNH

Sequences:

>Translated_358_residues
MKILVVDDSALMRTTISDILQNIPNAQIKTARDGMDAIDKVMKWQPDVMTLDINMPNMDGLTCLTQIMVERPLPIVMLSS
LTHEGAITTLEALYLGAVDFVAKPGGTICGRLQEVAPLIREKVRAAAKMRVKVHTQLSVSPAEVEHKHKSPVVENNEDHQ
LAIMGVSTGGPGTVETILRHLPADFSLPIIVNQHMPESFTAAFAQRLNRHLQQPVKEINRALVLEAGTVYVCKGDRDCIV
TLRDGKLAAMPTPNDSHFSWHPSVSKLVASAIRTCGEDNLLCVMLTGMGDDGANEMAQVAQGNGIVFAQEPTTCVVPSMP
EALLKRVPHIPTGTPEHLAYLMTEVVRQSSRELHYGNH
>Mature_358_residues
MKILVVDDSALMRTTISDILQNIPNAQIKTARDGMDAIDKVMKWQPDVMTLDINMPNMDGLTCLTQIMVERPLPIVMLSS
LTHEGAITTLEALYLGAVDFVAKPGGTICGRLQEVAPLIREKVRAAAKMRVKVHTQLSVSPAEVEHKHKSPVVENNEDHQ
LAIMGVSTGGPGTVETILRHLPADFSLPIIVNQHMPESFTAAFAQRLNRHLQQPVKEINRALVLEAGTVYVCKGDRDCIV
TLRDGKLAAMPTPNDSHFSWHPSVSKLVASAIRTCGEDNLLCVMLTGMGDDGANEMAQVAQGNGIVFAQEPTTCVVPSMP
EALLKRVPHIPTGTPEHLAYLMTEVVRQSSRELHYGNH

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=323, Percent_Identity=38.390092879257, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 39107; Mature: 39107

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00120 LIPASE_SER ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVVDDSALMRTTISDILQNIPNAQIKTARDGMDAIDKVMKWQPDVMTLDINMPNMDG
CEEEEECCHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHCCCCCEEEEEECCCCCCH
LTCLTQIMVERPLPIVMLSSLTHEGAITTLEALYLGAVDFVAKPGGTICGRLQEVAPLIR
HHHHHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EKVRAAAKMRVKVHTQLSVSPAEVEHKHKSPVVENNEDHQLAIMGVSTGGPGTVETILRH
HHHHHHHHEEEEEEEEECCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
LPADFSLPIIVNQHMPESFTAAFAQRLNRHLQQPVKEINRALVLEAGTVYVCKGDRDCIV
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEECCCCEEE
TLRDGKLAAMPTPNDSHFSWHPSVSKLVASAIRTCGEDNLLCVMLTGMGDDGANEMAQVA
EEECCEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
QGNGIVFAQEPTTCVVPSMPEALLKRVPHIPTGTPEHLAYLMTEVVRQSSRELHYGNH
CCCCEEEECCCCEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKILVVDDSALMRTTISDILQNIPNAQIKTARDGMDAIDKVMKWQPDVMTLDINMPNMDG
CEEEEECCHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHCCCCCEEEEEECCCCCCH
LTCLTQIMVERPLPIVMLSSLTHEGAITTLEALYLGAVDFVAKPGGTICGRLQEVAPLIR
HHHHHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EKVRAAAKMRVKVHTQLSVSPAEVEHKHKSPVVENNEDHQLAIMGVSTGGPGTVETILRH
HHHHHHHHEEEEEEEEECCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
LPADFSLPIIVNQHMPESFTAAFAQRLNRHLQQPVKEINRALVLEAGTVYVCKGDRDCIV
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEECCCCEEE
TLRDGKLAAMPTPNDSHFSWHPSVSKLVASAIRTCGEDNLLCVMLTGMGDDGANEMAQVA
EEECCEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
QGNGIVFAQEPTTCVVPSMPEALLKRVPHIPTGTPEHLAYLMTEVVRQSSRELHYGNH
CCCCEEEECCCCEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]