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The map label for this gene is yafC [C]

Identifier: 147675040

GI number: 147675040

Start: 1650277

End: 1651206

Strand: Reverse

Name: yafC [C]

Synonym: VC0395_A1536

Alternate gene names: 147675040

Gene position: 1651206-1650277 (Counterclockwise)

Preceding gene: 147673939

Following gene: 147674426

Centisome position: 54.6

GC content: 43.12

Gene sequence:

>930_bases
ATGAAAATAGAACCCAAAGTTACTAATAAAGGAACAGTCATGCTAAATGAGTTAAAACGCATAGTGATATTCAATAAAGT
GGTTGAGTGTGGTTCTTTTACTTGTGCAGCAGAAGCATTAGGTATGACCAAATCTAAGGTCAGTGAACAGATCACCGCTT
TAGAAAAAACACTGAATGTGCGCTTGCTAAACCGAACTACCCGTAAAATCAGTTTAACCACAGAGGGGGACACATTTTAT
CAACGCAGCCACGGATTATTGAATATTGCGGAGGAGGCGCTTTGCTCCGTTAACCACTTAGCGTCAGAAGTTTCCGGAAC
GATCCGCATTGGTACCACTATTGATGTCGGCACTTTTTTGCTCAACCCGCTACTGGCTGAGTTTTATAAGCAGTACCCTA
AGGTGAATTTCGATATTCAGCTCGATGATGGACTGCAAGATTTGGTGGATTCTAATCTGGATATGGTGATCCGAATTGGT
GAGCTGACGTCATCCTCTTTAGTTGGCCGTGTTATAGCGCCTTTTGAGTTAGGTATTTATGCCAGTCAAACCTATTTAGA
GAACGCGCCGCCGATCAATACGATTCAGGATTTAGAGCAGCACGATTGGGTCTATCTCACACGCTTAAATCTGCCTAACC
AAACCCTAACTTTAACCAATCAAGAGGGCGTACAACATCAGGTCAAATTCCATAGCAAACACAGCAGTAATTCTCCGCTT
GGCGTCATGACCATGGTGCAAAATGGTTTGGGTGTGGGATCGATTGCCAGTTTTTTGATTGATAAGTTGCCTGATGCATC
GCTAGTGCGATTGTTTCCGGACTTCTATCAGATCAGTACTACCATGAATATTCTCTACCCCAGTCGGAAAAATTTGCCGC
CAAGGGTGCGTTTATTTATCGATTATCTGGTGGGAACATTGTGTGGTTAA

Upstream 100 bases:

>100_bases
AAATCGTTTGGTTATATGAAATCTGCTTGAAACCAATAAAAATTACTCATCTCTAACAGGCGGAATTATTATTTCTGCTA
TTCCTTGTACTGACTAGATA

Downstream 100 bases:

>100_bases
TGGAGAGTGTTTAAACAGAACGCGCAATGAGCAGCATGGGTGTCGATGGCTTCTAGTGCCGGGGAAAGTCACCAAGCCCG
TTTAGGGCTTGATGAAGATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKIEPKVTNKGTVMLNELKRIVIFNKVVECGSFTCAAEALGMTKSKVSEQITALEKTLNVRLLNRTTRKISLTTEGDTFY
QRSHGLLNIAEEALCSVNHLASEVSGTIRIGTTIDVGTFLLNPLLAEFYKQYPKVNFDIQLDDGLQDLVDSNLDMVIRIG
ELTSSSLVGRVIAPFELGIYASQTYLENAPPINTIQDLEQHDWVYLTRLNLPNQTLTLTNQEGVQHQVKFHSKHSSNSPL
GVMTMVQNGLGVGSIASFLIDKLPDASLVRLFPDFYQISTTMNILYPSRKNLPPRVRLFIDYLVGTLCG

Sequences:

>Translated_309_residues
MKIEPKVTNKGTVMLNELKRIVIFNKVVECGSFTCAAEALGMTKSKVSEQITALEKTLNVRLLNRTTRKISLTTEGDTFY
QRSHGLLNIAEEALCSVNHLASEVSGTIRIGTTIDVGTFLLNPLLAEFYKQYPKVNFDIQLDDGLQDLVDSNLDMVIRIG
ELTSSSLVGRVIAPFELGIYASQTYLENAPPINTIQDLEQHDWVYLTRLNLPNQTLTLTNQEGVQHQVKFHSKHSSNSPL
GVMTMVQNGLGVGSIASFLIDKLPDASLVRLFPDFYQISTTMNILYPSRKNLPPRVRLFIDYLVGTLCG
>Mature_309_residues
MKIEPKVTNKGTVMLNELKRIVIFNKVVECGSFTCAAEALGMTKSKVSEQITALEKTLNVRLLNRTTRKISLTTEGDTFY
QRSHGLLNIAEEALCSVNHLASEVSGTIRIGTTIDVGTFLLNPLLAEFYKQYPKVNFDIQLDDGLQDLVDSNLDMVIRIG
ELTSSSLVGRVIAPFELGIYASQTYLENAPPINTIQDLEQHDWVYLTRLNLPNQTLTLTNQEGVQHQVKFHSKHSSNSPL
GVMTMVQNGLGVGSIASFLIDKLPDASLVRLFPDFYQISTTMNILYPSRKNLPPRVRLFIDYLVGTLCG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=33.1010452961672, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=30.0675675675676, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=29.8305084745763, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=27.4914089347079, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789440, Length=302, Percent_Identity=27.1523178807947, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=25.3424657534247, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787128, Length=300, Percent_Identity=25, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=24.8275862068966, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI157672245, Length=207, Percent_Identity=28.5024154589372, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788297, Length=144, Percent_Identity=29.1666666666667, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788706, Length=306, Percent_Identity=23.5294117647059, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788748, Length=120, Percent_Identity=31.6666666666667, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789173, Length=204, Percent_Identity=28.4313725490196, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=267, Percent_Identity=22.8464419475655, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=144, Percent_Identity=30.5555555555556, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=269, Percent_Identity=24.1635687732342, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789506, Length=219, Percent_Identity=23.2876712328767, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34395; Mature: 34395

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEPKVTNKGTVMLNELKRIVIFNKVVECGSFTCAAEALGMTKSKVSEQITALEKTLNV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHE
RLLNRTTRKISLTTEGDTFYQRSHGLLNIAEEALCSVNHLASEVSGTIRIGTTIDVGTFL
EEEECCCEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCEECHHHHH
LNPLLAEFYKQYPKVNFDIQLDDGLQDLVDSNLDMVIRIGELTSSSLVGRVIAPFELGIY
HHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCHHCEE
ASQTYLENAPPINTIQDLEQHDWVYLTRLNLPNQTLTLTNQEGVQHQVKFHSKHSSNSPL
ECHHHHCCCCCCCHHHHHHCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHCCCCCCCCC
GVMTMVQNGLGVGSIASFLIDKLPDASLVRLFPDFYQISTTMNILYPSRKNLPPRVRLFI
CHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHH
DYLVGTLCG
HHHHHHHCC
>Mature Secondary Structure
MKIEPKVTNKGTVMLNELKRIVIFNKVVECGSFTCAAEALGMTKSKVSEQITALEKTLNV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHE
RLLNRTTRKISLTTEGDTFYQRSHGLLNIAEEALCSVNHLASEVSGTIRIGTTIDVGTFL
EEEECCCEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCEECHHHHH
LNPLLAEFYKQYPKVNFDIQLDDGLQDLVDSNLDMVIRIGELTSSSLVGRVIAPFELGIY
HHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCHHCEE
ASQTYLENAPPINTIQDLEQHDWVYLTRLNLPNQTLTLTNQEGVQHQVKFHSKHSSNSPL
ECHHHHCCCCCCCHHHHHHCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHCCCCCCCCC
GVMTMVQNGLGVGSIASFLIDKLPDASLVRLFPDFYQISTTMNILYPSRKNLPPRVRLFI
CHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHH
DYLVGTLCG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]