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The map label for this gene is dam

Identifier: 147675035

GI number: 147675035

Start: 2350789

End: 2351622

Strand: Reverse

Name: dam

Synonym: VC0395_A2203

Alternate gene names: 147675035

Gene position: 2351622-2350789 (Counterclockwise)

Preceding gene: 147675481

Following gene: 147674230

Centisome position: 77.76

GC content: 48.32

Gene sequence:

>834_bases
ATGAAAAAGCAGCGCGCCTTTCTGAAATGGGCTGGGGGTAAATATAGCCTAGTCGAGGATATTCAACGCCATCTACCAGA
AGCTCGTGAGCTGGTTGAGCCTTTCGTTGGTGCAGGCTCGGTCTTCCTGAATACGGATTTTGAACGCTATCTGCTGGCGG
ACATCAATCCGGATCTGATCAATTTCTACAATCTGCTCAAAACCGAACCTCAAGCTTACATTCACGAAGCTAAGCGTTGG
TTTGTGCCCGAAAATAACCGCAAAGAAGTCTACCTCGATATTCGTAAGCAGTTTAACCAGAGTGACGATGCGATGTTTCG
CTCGTTGGCGTTTTTGTATATGAACCGCTTTGGGTTTAACGGCCTATGTCGTTATAACAAAAAAGGGGGCTTTAATGTGC
CGTTTGGCTCGTACAAAAAGCCTTATTTCCCTGAGCAAGAGTTAGAGTTTTTTGCTGAAAAAGCGCAGCGTGCCACCTTT
ATTTGCGCGTCGTATGGCGAAACCTTCGCTCGCGCGCAAAGCGATAGCGTGATTTATTGCGATCCACCTTACGCACCGCT
GTCGACTACGGCCAATTTTACCTCTTATGCGGGCAATGGTTTTACCTTAGACGATCAAGCTGCGTTAGCCGATATCGCCG
AGAAAACCGCCAAAGAGCGTGGAATTTCGGTGCTGATTTCCAATCACGACACCACCCATACTCGCCGTTTGTATCGCGGT
GCGCAGCTCAATGTGGTCAAAGCTAACCGTACCATCAGTCGTAATGGTGCCGGACGCAATAAAGTGGATGAGCTTTTAGC
ACTCTTCACGCCTCATCTTTCATCGCAAGCGTGA

Upstream 100 bases:

>100_bases
AGTTCAGCGAGAAATCGAGCGTAAGAAATAAACCTGAGCTTCGCTGAAAAATATGTTACATTCCGCAGCCTTATTTTGTG
GCCAACGATTAGAGCAGTAG

Downstream 100 bases:

>100_bases
TCTTGTGGGCTGAATGGTGGATTTCCTCTCCCTGCCTCAAGTCACGCGCTAGGATCTCGTCACCAGCTTAGGTAGAATGG
CGGCCACATTGTGTTCAGCT

Product: DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.VchADam

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKKQRAFLKWAGGKYSLVEDIQRHLPEARELVEPFVGAGSVFLNTDFERYLLADINPDLINFYNLLKTEPQAYIHEAKRW
FVPENNRKEVYLDIRKQFNQSDDAMFRSLAFLYMNRFGFNGLCRYNKKGGFNVPFGSYKKPYFPEQELEFFAEKAQRATF
ICASYGETFARAQSDSVIYCDPPYAPLSTTANFTSYAGNGFTLDDQAALADIAEKTAKERGISVLISNHDTTHTRRLYRG
AQLNVVKANRTISRNGAGRNKVDELLALFTPHLSSQA

Sequences:

>Translated_277_residues
MKKQRAFLKWAGGKYSLVEDIQRHLPEARELVEPFVGAGSVFLNTDFERYLLADINPDLINFYNLLKTEPQAYIHEAKRW
FVPENNRKEVYLDIRKQFNQSDDAMFRSLAFLYMNRFGFNGLCRYNKKGGFNVPFGSYKKPYFPEQELEFFAEKAQRATF
ICASYGETFARAQSDSVIYCDPPYAPLSTTANFTSYAGNGFTLDDQAALADIAEKTAKERGISVLISNHDTTHTRRLYRG
AQLNVVKANRTISRNGAGRNKVDELLALFTPHLSSQA
>Mature_277_residues
MKKQRAFLKWAGGKYSLVEDIQRHLPEARELVEPFVGAGSVFLNTDFERYLLADINPDLINFYNLLKTEPQAYIHEAKRW
FVPENNRKEVYLDIRKQFNQSDDAMFRSLAFLYMNRFGFNGLCRYNKKGGFNVPFGSYKKPYFPEQELEFFAEKAQRATF
ICASYGETFARAQSDSVIYCDPPYAPLSTTANFTSYAGNGFTLDDQAALADIAEKTAKERGISVLISNHDTTHTRRLYRG
AQLNVVKANRTISRNGAGRNKVDELLALFTPHLSSQA

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789789, Length=277, Percent_Identity=63.5379061371841, Blast_Score=361, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DMA_VIBC3 (A5F520)

Other databases:

- EMBL:   AF274317
- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F520
- SMR:   A5F520
- STRING:   A5F520
- REBASE:   15105
- REBASE:   20804
- REBASE:   5786
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2203
- eggNOG:   COG0338
- HOGENOM:   HBG586381
- OMA:   AFEQTRS
- ProtClustDB:   CLSK874863
- BioCyc:   VCHO345073:VC0395_A2203-MONOMER
- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327
- Gene3D:   G3DSA:1.10.1020.10
- PRINTS:   PR00505
- TIGRFAMs:   TIGR00571

Pfam domain/function: PF02086 MethyltransfD12

EC number: =2.1.1.72

Molecular weight: Translated: 31624; Mature: 31624

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 10-10 BINDING 14-14 BINDING 54-54 BINDING 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQRAFLKWAGGKYSLVEDIQRHLPEARELVEPFVGAGSVFLNTDFERYLLADINPDLI
CCCCCCCEEECCCCCHHHHHHHHHCHHHHHHHHHHHCCCEEEEECCHHHEEEECCCHHHH
NFYNLLKTEPQAYIHEAKRWFVPENNRKEVYLDIRKQFNQSDDAMFRSLAFLYMNRFGFN
HHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCC
GLCRYNKKGGFNVPFGSYKKPYFPEQELEFFAEKAQRATFICASYGETFARAQSDSVIYC
CEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCCEEEE
DPPYAPLSTTANFTSYAGNGFTLDDQAALADIAEKTAKERGISVLISNHDTTHTRRLYRG
CCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCC
AQLNVVKANRTISRNGAGRNKVDELLALFTPHLSSQA
CEEEEEECCCEECCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKQRAFLKWAGGKYSLVEDIQRHLPEARELVEPFVGAGSVFLNTDFERYLLADINPDLI
CCCCCCCEEECCCCCHHHHHHHHHCHHHHHHHHHHHCCCEEEEECCHHHEEEECCCHHHH
NFYNLLKTEPQAYIHEAKRWFVPENNRKEVYLDIRKQFNQSDDAMFRSLAFLYMNRFGFN
HHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCC
GLCRYNKKGGFNVPFGSYKKPYFPEQELEFFAEKAQRATFICASYGETFARAQSDSVIYC
CEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCCEEEE
DPPYAPLSTTANFTSYAGNGFTLDDQAALADIAEKTAKERGISVLISNHDTTHTRRLYRG
CCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCC
AQLNVVKANRTISRNGAGRNKVDELLALFTPHLSSQA
CEEEEEECCCEECCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11705940