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The map label for this gene is dsbE

Identifier: 147675010

GI number: 147675010

Start: 1761053

End: 1761607

Strand: Reverse

Name: dsbE

Synonym: VC0395_A1639

Alternate gene names: 147675010

Gene position: 1761607-1761053 (Counterclockwise)

Preceding gene: 147674378

Following gene: 147675051

Centisome position: 58.25

GC content: 45.05

Gene sequence:

>555_bases
GTGAATAAGAAGATTTTATTTATCCCGCTAGTGGCATTTTTGCTGTTGGCTGGTGTGTTTGCTACCCAGTTAATGAAAAA
CTCCGCAGGGGATGACCCAACCAAGTTGGAATCGGTTTTGGTGGGAAAAACCGTTCCTGAGTTTCGTCTCGAAGATCTGG
CAGAGCCCGGTAAGTTATACGATCAGTCGATTTTCAAAGGCGAGCCTTTACTGCTTAATGTATGGGCAACCTGGTGTCCA
ACTTGTTATGCCGAGCATCAATATCTCAATGAGTTGGCCAAACAAGGGGTAAAAATCATCGGCCTTAATTATAAAGATCA
GCGAGATAAAGCAACTCAATGGCTAAATGATTTAGGCAATCCTTACCTGATCAGCTTGTTTGATGGCAATGGTATGTTGG
GACTGGATCTTGGGGTGTACGGTGCTCCAGAAACCTTCTTAATCGATGCCAATGGCGTGATTCGCTATCGTCATGTTGGG
GATGTTAATTCGCGCAACTGGCAAGAAACACTCGCGCCACTGTATGAAAAAATGCTGGCGGAGGCGAAACAATGA

Upstream 100 bases:

>100_bases
CTGGATTTGGGCAGGCGGCTTGCTGATGGCCATCGGTGGTGCCTTAGCCATCAGTGACAAGCGTTACCGCTTTAGAAAAA
ACGCACATCAGGAGGCTTAA

Downstream 100 bases:

>100_bases
AAAAGTGGTTGTTTGCGGTCTGTGCCGCCATGCTGATTTCTCTCTCGGCGTTTGCAACGATTGAAGTGTATGACTTTGAT
ACTCCAGAGCAGGAGAAACA

Product: thiol:disulfide interchange protein DsbE

Products: NA

Alternate protein names: Cytochrome c biogenesis protein CcmG

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MNKKILFIPLVAFLLLAGVFATQLMKNSAGDDPTKLESVLVGKTVPEFRLEDLAEPGKLYDQSIFKGEPLLLNVWATWCP
TCYAEHQYLNELAKQGVKIIGLNYKDQRDKATQWLNDLGNPYLISLFDGNGMLGLDLGVYGAPETFLIDANGVIRYRHVG
DVNSRNWQETLAPLYEKMLAEAKQ

Sequences:

>Translated_184_residues
MNKKILFIPLVAFLLLAGVFATQLMKNSAGDDPTKLESVLVGKTVPEFRLEDLAEPGKLYDQSIFKGEPLLLNVWATWCP
TCYAEHQYLNELAKQGVKIIGLNYKDQRDKATQWLNDLGNPYLISLFDGNGMLGLDLGVYGAPETFLIDANGVIRYRHVG
DVNSRNWQETLAPLYEKMLAEAKQ
>Mature_184_residues
MNKKILFIPLVAFLLLAGVFATQLMKNSAGDDPTKLESVLVGKTVPEFRLEDLAEPGKLYDQSIFKGEPLLLNVWATWCP
TCYAEHQYLNELAKQGVKIIGLNYKDQRDKATQWLNDLGNPYLISLFDGNGMLGLDLGVYGAPETFLIDANGVIRYRHVG
DVNSRNWQETLAPLYEKMLAEAKQ

Specific function: Involved in disulfide bond formation. Catalyzes a late, reductive step in the assembly of periplasmic c-type cytochromes, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain

Homologues:

Organism=Escherichia coli, GI1788523, Length=185, Percent_Identity=62.7027027027027, Blast_Score=259, Evalue=8e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DSBE_VIBCH (Q9KQE9)

Other databases:

- EMBL:   AE003852
- PIR:   E82124
- RefSeq:   NP_231683.1
- ProteinModelPortal:   Q9KQE9
- SMR:   Q9KQE9
- GeneID:   2613431
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2051
- TIGR:   VC_2051
- HOGENOM:   HBG493509
- OMA:   KWLAEFH
- ProtClustDB:   CLSK874655
- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- TIGRFAMs:   TIGR00385

Pfam domain/function: PF08534 Redoxin; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 20651; Mature: 20651

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00194 THIOREDOXIN_1; PS51352 THIOREDOXIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1a6987e4)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKILFIPLVAFLLLAGVFATQLMKNSAGDDPTKLESVLVGKTVPEFRLEDLAEPGKLY
CCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHH
DQSIFKGEPLLLNVWATWCPTCYAEHQYLNELAKQGVKIIGLNYKDQRDKATQWLNDLGN
HHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCC
PYLISLFDGNGMLGLDLGVYGAPETFLIDANGVIRYRHVGDVNSRNWQETLAPLYEKMLA
CEEEEEECCCCEEEEECCCCCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHH
EAKQ
HHCH
>Mature Secondary Structure
MNKKILFIPLVAFLLLAGVFATQLMKNSAGDDPTKLESVLVGKTVPEFRLEDLAEPGKLY
CCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHH
DQSIFKGEPLLLNVWATWCPTCYAEHQYLNELAKQGVKIIGLNYKDQRDKATQWLNDLGN
HHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCC
PYLISLFDGNGMLGLDLGVYGAPETFLIDANGVIRYRHVGDVNSRNWQETLAPLYEKMLA
CEEEEEECCCCEEEEECCCCCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHH
EAKQ
HHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301