Click here to switch to the map view.

The map label for this gene is iciA

Identifier: 147674972

GI number: 147674972

Start: 30623

End: 31519

Strand: Direct

Name: iciA

Synonym: VC0395_A0034

Alternate gene names: 147674972

Gene position: 30623-31519 (Clockwise)

Preceding gene: 147673216

Following gene: 147674164

Centisome position: 1.01

GC content: 46.71

Gene sequence:

>897_bases
ATGCGTGGTCTGGATTATCGGTGGATTGAAGCTCTAGATAGTGTGGTGAGTAAAGGCAGTTTTGAACGAGCGGCTGAGCA
GCTCTTTATCTCCCAATCTGCTGTATCACAACGGATTAAACAGTTAGAAAAATACTTAGCTCAGCCAGTGTTGATCCGTG
AGCAGCCACCAAGGCCAACCTTGGTTGGCAAAAAGTTGCTTGGGCTCTATCGCCGAGTCTGTTTAATCGAACAAGAGTTG
GTTCCGGAGTTGACGAACCAAGAGCATGTTCGCCCGGTTTCTATGTCGATTGCGACCAATGCAGATAGTCTAGCCACTTG
GTTGTTACCGGCGCTGGATAAGGTAATGAAAAGCCGCCAAGTGGAATTGAATCTGGTGATTTATGGTGAGTCACGTACCC
TCGATAAGCTCAAAAATGGTGAAGTGGTCGGTGCAATCAGTTTGGAGCCACAACCGATCACTGGGTGTAGCGCTGAGTAT
TTAGGCCAGATGGAGTATCTGTGCGTCGCAAGCCCAGAGTTCTATCAAAAATACTTTGCTAAGGGGGTCACCCCGCGTTC
ATTAATCAAAGCGCCTGCGGTGTCTTACGATCAATATGACGAATTGCACAATAAATTCTTATGGGATTACTTTGCTGTAC
TACGTGATAAAGTCATCAACCACACGGTTGGCAGCTCAGAAGCTTTTGTCCGTTTAGCGCTTTCTGGCGCGGCTTACTGT
TTGATCCCACGTTTGCAGATCATTTCGGAATTGGAAAGCGGTGCTCTCATCAATATGACGCCTGATTTTATGCTGTCATA
TCCGATTTTTTGGCATCATTGGCAGCTTGAAACGGGCGTCTTACTTGAAATTAGCGACGCGATCACCGCTTATGCGAAAA
GCGTACTACCTCAATAA

Upstream 100 bases:

>100_bases
CGAAGGGATAGGCTGAGCAAGATAATAATTTGAGGTGGATAAGTATAATTAATGATTTTAATATCATATTAACAAGGCTA
ATGAATAGATAAGGAAAGCA

Downstream 100 bases:

>100_bases
TTGACCGAGTCAAAGTTGTGTAATTTTTATGCGTAGAGACTGAATCTATGCACTGCTTCGCTTATGATGTGGGCAGTCGA
TGACATTAAGGATCGAAACG

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MRGLDYRWIEALDSVVSKGSFERAAEQLFISQSAVSQRIKQLEKYLAQPVLIREQPPRPTLVGKKLLGLYRRVCLIEQEL
VPELTNQEHVRPVSMSIATNADSLATWLLPALDKVMKSRQVELNLVIYGESRTLDKLKNGEVVGAISLEPQPITGCSAEY
LGQMEYLCVASPEFYQKYFAKGVTPRSLIKAPAVSYDQYDELHNKFLWDYFAVLRDKVINHTVGSSEAFVRLALSGAAYC
LIPRLQIISELESGALINMTPDFMLSYPIFWHHWQLETGVLLEISDAITAYAKSVLPQ

Sequences:

>Translated_298_residues
MRGLDYRWIEALDSVVSKGSFERAAEQLFISQSAVSQRIKQLEKYLAQPVLIREQPPRPTLVGKKLLGLYRRVCLIEQEL
VPELTNQEHVRPVSMSIATNADSLATWLLPALDKVMKSRQVELNLVIYGESRTLDKLKNGEVVGAISLEPQPITGCSAEY
LGQMEYLCVASPEFYQKYFAKGVTPRSLIKAPAVSYDQYDELHNKFLWDYFAVLRDKVINHTVGSSEAFVRLALSGAAYC
LIPRLQIISELESGALINMTPDFMLSYPIFWHHWQLETGVLLEISDAITAYAKSVLPQ
>Mature_298_residues
MRGLDYRWIEALDSVVSKGSFERAAEQLFISQSAVSQRIKQLEKYLAQPVLIREQPPRPTLVGKKLLGLYRRVCLIEQEL
VPELTNQEHVRPVSMSIATNADSLATWLLPALDKVMKSRQVELNLVIYGESRTLDKLKNGEVVGAISLEPQPITGCSAEY
LGQMEYLCVASPEFYQKYFAKGVTPRSLIKAPAVSYDQYDELHNKFLWDYFAVLRDKVINHTVGSSEAFVRLALSGAAYC
LIPRLQIISELESGALINMTPDFMLSYPIFWHHWQLETGVLLEISDAITAYAKSVLPQ

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789283, Length=298, Percent_Identity=46.6442953020134, Blast_Score=270, Evalue=8e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ICIA_VIBC3 (A5F9F1)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F9F1
- STRING:   A5F9F1
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0034
- eggNOG:   COG0583
- HOGENOM:   HBG685425
- OMA:   YLFVASP
- ProtClustDB:   PRK03635
- BioCyc:   VCHO345073:VC0395_A0034-MONOMER
- HAMAP:   MF_00513
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039
- TIGRFAMs:   TIGR03298

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33655; Mature: 33655

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLDYRWIEALDSVVSKGSFERAAEQLFISQSAVSQRIKQLEKYLAQPVLIREQPPRPT
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCH
LVGKKLLGLYRRVCLIEQELVPELTNQEHVRPVSMSIATNADSLATWLLPALDKVMKSRQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCC
VELNLVIYGESRTLDKLKNGEVVGAISLEPQPITGCSAEYLGQMEYLCVASPEFYQKYFA
EEEEEEEEECCCCHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCEEEECCHHHHHHHHH
KGVTPRSLIKAPAVSYDQYDELHNKFLWDYFAVLRDKVINHTVGSSEAFVRLALSGAAYC
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHHHCCCHHH
LIPRLQIISELESGALINMTPDFMLSYPIFWHHWQLETGVLLEISDAITAYAKSVLPQ
HHHHHHHHHHHCCCCEEEECHHHHHCCCCEEEEEEECCCEEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MRGLDYRWIEALDSVVSKGSFERAAEQLFISQSAVSQRIKQLEKYLAQPVLIREQPPRPT
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCH
LVGKKLLGLYRRVCLIEQELVPELTNQEHVRPVSMSIATNADSLATWLLPALDKVMKSRQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCC
VELNLVIYGESRTLDKLKNGEVVGAISLEPQPITGCSAEYLGQMEYLCVASPEFYQKYFA
EEEEEEEEECCCCHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCEEEECCHHHHHHHHH
KGVTPRSLIKAPAVSYDQYDELHNKFLWDYFAVLRDKVINHTVGSSEAFVRLALSGAAYC
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHHHCCCHHH
LIPRLQIISELESGALINMTPDFMLSYPIFWHHWQLETGVLLEISDAITAYAKSVLPQ
HHHHHHHHHHHCCCCEEEECHHHHHCCCCEEEEEEECCCEEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA