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The map label for this gene is truA

Identifier: 147674772

GI number: 147674772

Start: 560701

End: 561495

Strand: Direct

Name: truA

Synonym: VC0395_A0520

Alternate gene names: 147674772

Gene position: 560701-561495 (Clockwise)

Preceding gene: 147673505

Following gene: 147674043

Centisome position: 18.54

GC content: 50.82

Gene sequence:

>795_bases
ATGAGAATCGCGTTAGGTATTGAGTATGACGGGACTCACTACTACGGTTGGCAGCGCCAGAGAGAAGTCAAAAGTGTCCA
AGAAGCGTTAGAAAAGGCGCTGAGTAAGATTGCCAATCATCCAGTGGAAGTGCAGTGTGCTGGGCGAACTGATGCGGGGG
TACATGGTACTGGGCAAGTGGTGCACTTTGATACCACCGCCGAACGGCAGATGGTCGCTTGGACCATGGGCGCCAACGCG
AATTTGCCGAAGGATATTGCAGTACGCTGGGCGATGGCGGTTCCTGATGAATTTCATGCACGATTCAGTGCCACTGCACG
CCGTTATCGTTATGTCATTTACAACCATGTTTATCGCCCGGGGATTTTGAATTCTGGTGTCAGCCATTACCACGGCGAGC
TGGATGTTGAAAAAATGCAGCAAGCAGGGCAGTACTTGCTGGGGGAAAATGATTTCACCTCATTTCGTGCGGTGCATTGT
CAGTCCCGTAGTCCTTGGCGTAACGTCATGCATCTGAATGTGACGCGTCACGGTCGTTATGTGGTGATTGATATTAAAGC
CAATGCGTTCGTGCATCATATGGTACGCAATATCACGGGTAGCTTGATAGCCGTCGGGCGTGGTGAGCAGAAGCCAGAGT
GGATTCAATGGTTATTGGCACAAAAAGATCGCACCTTAGCGGCGGCGACGGCCAAAGCGGAGGGGCTCTACTTGGTTTCT
GTTGATTACCCTGCGCATTTTGGGTTGCCCGAAATGCCAATTGGGCCACTTTTCTTGCCTGATAATTTGAACTAA

Upstream 100 bases:

>100_bases
CTTTAAAAGGCAGCTTAGCTGCCTTTTACTTTTGTAGTGATTAGAAATTTACTGGCAAACTGTTTATACTTCCCGACCCA
TTTTTATTGAGACAAACAGC

Downstream 100 bases:

>100_bases
CAGCTAGTAATTGACTCAAAAATAAACAATATTGTGCTTTGGCTAAAGCGCAGAAATAGGTACAACCAGTTTTTTGTCTA
CAGTTGGCATTTTCTGTGTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRIALGIEYDGTHYYGWQRQREVKSVQEALEKALSKIANHPVEVQCAGRTDAGVHGTGQVVHFDTTAERQMVAWTMGANA
NLPKDIAVRWAMAVPDEFHARFSATARRYRYVIYNHVYRPGILNSGVSHYHGELDVEKMQQAGQYLLGENDFTSFRAVHC
QSRSPWRNVMHLNVTRHGRYVVIDIKANAFVHHMVRNITGSLIAVGRGEQKPEWIQWLLAQKDRTLAAATAKAEGLYLVS
VDYPAHFGLPEMPIGPLFLPDNLN

Sequences:

>Translated_264_residues
MRIALGIEYDGTHYYGWQRQREVKSVQEALEKALSKIANHPVEVQCAGRTDAGVHGTGQVVHFDTTAERQMVAWTMGANA
NLPKDIAVRWAMAVPDEFHARFSATARRYRYVIYNHVYRPGILNSGVSHYHGELDVEKMQQAGQYLLGENDFTSFRAVHC
QSRSPWRNVMHLNVTRHGRYVVIDIKANAFVHHMVRNITGSLIAVGRGEQKPEWIQWLLAQKDRTLAAATAKAEGLYLVS
VDYPAHFGLPEMPIGPLFLPDNLN
>Mature_264_residues
MRIALGIEYDGTHYYGWQRQREVKSVQEALEKALSKIANHPVEVQCAGRTDAGVHGTGQVVHFDTTAERQMVAWTMGANA
NLPKDIAVRWAMAVPDEFHARFSATARRYRYVIYNHVYRPGILNSGVSHYHGELDVEKMQQAGQYLLGENDFTSFRAVHC
QSRSPWRNVMHLNVTRHGRYVVIDIKANAFVHHMVRNITGSLIAVGRGEQKPEWIQWLLAQKDRTLAAATAKAEGLYLVS
VDYPAHFGLPEMPIGPLFLPDNLN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=268, Percent_Identity=29.4776119402985, Blast_Score=95, Evalue=5e-20,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=73.8461538461539, Blast_Score=409, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321112, Length=243, Percent_Identity=26.7489711934156, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648455, Length=263, Percent_Identity=24.3346007604563, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281362146, Length=263, Percent_Identity=24.3346007604563, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_VIBC3 (A5F2T4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F2T4
- SMR:   A5F2T4
- STRING:   A5F2T4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0520
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- ProtClustDB:   PRK00021
- BioCyc:   VCHO345073:VC0395_A0520-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29847; Mature: 29847

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALGIEYDGTHYYGWQRQREVKSVQEALEKALSKIANHPVEVQCAGRTDAGVHGTGQV
CEEEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCE
VHFDTTAERQMVAWTMGANANLPKDIAVRWAMAVPDEFHARFSATARRYRYVIYNHVYRP
EEEECCCCCEEEEEEECCCCCCCHHHHEEEEECCCHHHHHHHHHHHHHHEEEEECCCCCC
GILNSGVSHYHGELDVEKMQQAGQYLLGENDFTSFRAVHCQSRSPWRNVMHLNVTRHGRY
CCCCCCHHHHCCCCCHHHHHHCCCEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCE
VVIDIKANAFVHHMVRNITGSLIAVGRGEQKPEWIQWLLAQKDRTLAAATAKAEGLYLVS
EEEEEECCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEEEE
VDYPAHFGLPEMPIGPLFLPDNLN
ECCCCCCCCCCCCCCCEECCCCCC
>Mature Secondary Structure
MRIALGIEYDGTHYYGWQRQREVKSVQEALEKALSKIANHPVEVQCAGRTDAGVHGTGQV
CEEEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCE
VHFDTTAERQMVAWTMGANANLPKDIAVRWAMAVPDEFHARFSATARRYRYVIYNHVYRP
EEEECCCCCEEEEEEECCCCCCCHHHHEEEEECCCHHHHHHHHHHHHHHEEEEECCCCCC
GILNSGVSHYHGELDVEKMQQAGQYLLGENDFTSFRAVHCQSRSPWRNVMHLNVTRHGRY
CCCCCCHHHHCCCCCHHHHHHCCCEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCE
VVIDIKANAFVHHMVRNITGSLIAVGRGEQKPEWIQWLLAQKDRTLAAATAKAEGLYLVS
EEEEEECCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEEEE
VDYPAHFGLPEMPIGPLFLPDNLN
ECCCCCCCCCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA