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The map label for this gene is cysB [H]

Identifier: 147674603

GI number: 147674603

Start: 1611058

End: 1612032

Strand: Reverse

Name: cysB [H]

Synonym: VC0395_A1497

Alternate gene names: 147674603

Gene position: 1612032-1611058 (Counterclockwise)

Preceding gene: 147675397

Following gene: 147674493

Centisome position: 53.31

GC content: 46.46

Gene sequence:

>975_bases
ATGAAACTGCAGCAGTTAAAGTACATTGTGGAAGTGGTCAATCATAATTTGAATGTGTCTGCGACCGCGGAAAACCTATA
TACCTCTCAGCCGGGGATCAGTAAACAGGTCAGGCTGTTAGAAGATGAGCTTGGAATTCAGATTTTTGAGCGAAGCGGCA
AGCATTTAACGCAAGTAACGCCTGCGGGCGAGCACATAGTGCGTATTGCGACGGAAATTTTATCGCGCGTCGAAAGCATC
AAGGCGGTTGCTGCCGAGCATACGCATCCAGAAATGGGCACGCTGAATATTTCCACTACCCATACCCAAGCCCGCTATGC
TCTGCCAGAGGTGATTAAAGGTTTTACTAAGCGTTATCCAAGAGTTTCCTTGCACATGCATCAAGGCACGCCAACTCAAA
TGTCGGAAGCGATAGCCAAAGGCACTGCAAATTTTGCGATTGCGACAGAAGCTTTGCATTTGTACCAAGATGCAATCATG
TTGCCTTGTTATCACTGGAATCGTTCGATAGTGGTGCCTAAAGATCACCCGCTGACCAAGAAAGAGCAGGTGACCATAGA
AGATTTAGCGGCTTATCCATTAGTGACCTATGTTTTCGGCTTTACTGGGCGTTCAGAGCTTGATAGTGCATTCAATCGGG
TTGGCATGACACCTAAAGTGGTCTTTACCGCCACCGATGCAGATGTGATAAAAACGTATGTGCGCATGGGTATTGGGGTG
GGTGTGATTGCTAGTATGGCGGTAGACAAAAAGCAAGATACGGATCTGGTGGCGATTGATGCAAGCCATATCTTCAGTGC
GAGTACCACCAGTATTGGTTTTCGCCGTGGTACTTTCCTGCGCTCTTATATGTTTGATTTTATGGAGCGCTTTGCTCCAC
ATCTTACCCGTCCCGTAGTAGAGCAAGCCATCTCTTTGAAATCGAATATTGAAATAGCAGAAATGTTTAAGGACATTGAT
CTGCCTGTGCGTTAA

Upstream 100 bases:

>100_bases
CTCGATAAAGGCTAGGAATTGTCGCGCTGAAGCGTTATAACTAATAATTATAAAATATGTGTGTCTGTGGCAAAACACCG
CAAGGGATTATGGGAAAAAG

Downstream 100 bases:

>100_bases
CGCGCTTTTACTTTCACTTCATTGTGCTTCCTCCTAGAATGGTTCACCGATAGGAGGAAGCATGCAAGCTCTATTCATAC
AACTCGATTCATTCTTAACT

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLQQLKYIVEVVNHNLNVSATAENLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVTPAGEHIVRIATEILSRVESI
KAVAAEHTHPEMGTLNISTTHTQARYALPEVIKGFTKRYPRVSLHMHQGTPTQMSEAIAKGTANFAIATEALHLYQDAIM
LPCYHWNRSIVVPKDHPLTKKEQVTIEDLAAYPLVTYVFGFTGRSELDSAFNRVGMTPKVVFTATDADVIKTYVRMGIGV
GVIASMAVDKKQDTDLVAIDASHIFSASTTSIGFRRGTFLRSYMFDFMERFAPHLTRPVVEQAISLKSNIEIAEMFKDID
LPVR

Sequences:

>Translated_324_residues
MKLQQLKYIVEVVNHNLNVSATAENLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVTPAGEHIVRIATEILSRVESI
KAVAAEHTHPEMGTLNISTTHTQARYALPEVIKGFTKRYPRVSLHMHQGTPTQMSEAIAKGTANFAIATEALHLYQDAIM
LPCYHWNRSIVVPKDHPLTKKEQVTIEDLAAYPLVTYVFGFTGRSELDSAFNRVGMTPKVVFTATDADVIKTYVRMGIGV
GVIASMAVDKKQDTDLVAIDASHIFSASTTSIGFRRGTFLRSYMFDFMERFAPHLTRPVVEQAISLKSNIEIAEMFKDID
LPVR
>Mature_324_residues
MKLQQLKYIVEVVNHNLNVSATAENLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVTPAGEHIVRIATEILSRVESI
KAVAAEHTHPEMGTLNISTTHTQARYALPEVIKGFTKRYPRVSLHMHQGTPTQMSEAIAKGTANFAIATEALHLYQDAIM
LPCYHWNRSIVVPKDHPLTKKEQVTIEDLAAYPLVTYVFGFTGRSELDSAFNRVGMTPKVVFTATDADVIKTYVRMGIGV
GVIASMAVDKKQDTDLVAIDASHIFSASTTSIGFRRGTFLRSYMFDFMERFAPHLTRPVVEQAISLKSNIEIAEMFKDID
LPVR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=322, Percent_Identity=76.0869565217391, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1788296, Length=289, Percent_Identity=42.2145328719723, Blast_Score=260, Evalue=9e-71,
Organism=Escherichia coli, GI1790399, Length=286, Percent_Identity=25.8741258741259, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI157672245, Length=235, Percent_Identity=26.3829787234043, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=28.8617886178862, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787806, Length=285, Percent_Identity=24.2105263157895, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787879, Length=251, Percent_Identity=25.4980079681275, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790262, Length=249, Percent_Identity=24.8995983935743, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=186, Percent_Identity=25.2688172043011, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=28.1879194630872, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI2367136, Length=251, Percent_Identity=23.1075697211155, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788748, Length=258, Percent_Identity=21.7054263565891, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789204, Length=226, Percent_Identity=23.0088495575221, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788481, Length=253, Percent_Identity=24.1106719367589, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36173; Mature: 36173

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLKYIVEVVNHNLNVSATAENLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVT
CCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHCHHHHHCCCCEEEEEC
PAGEHIVRIATEILSRVESIKAVAAEHTHPEMGTLNISTTHTQARYALPEVIKGFTKRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCC
RVSLHMHQGTPTQMSEAIAKGTANFAIATEALHLYQDAIMLPCYHWNRSIVVPKDHPLTK
CEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCEEEECCCEEECCCCCCCCC
KEQVTIEDLAAYPLVTYVFGFTGRSELDSAFNRVGMTPKVVFTATDADVIKTYVRMGIGV
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCH
GVIASMAVDKKQDTDLVAIDASHIFSASTTSIGFRRGTFLRSYMFDFMERFAPHLTRPVV
HHHHHHHHCCCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQAISLKSNIEIAEMFKDIDLPVR
HHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLQQLKYIVEVVNHNLNVSATAENLYTSQPGISKQVRLLEDELGIQIFERSGKHLTQVT
CCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHCHHHHHCCCCEEEEEC
PAGEHIVRIATEILSRVESIKAVAAEHTHPEMGTLNISTTHTQARYALPEVIKGFTKRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCC
RVSLHMHQGTPTQMSEAIAKGTANFAIATEALHLYQDAIMLPCYHWNRSIVVPKDHPLTK
CEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCEEEECCCEEECCCCCCCCC
KEQVTIEDLAAYPLVTYVFGFTGRSELDSAFNRVGMTPKVVFTATDADVIKTYVRMGIGV
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCH
GVIASMAVDKKQDTDLVAIDASHIFSASTTSIGFRRGTFLRSYMFDFMERFAPHLTRPVV
HHHHHHHHCCCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQAISLKSNIEIAEMFKDIDLPVR
HHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]