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The map label for this gene is fliF [H]

Identifier: 147674591

GI number: 147674591

Start: 1839655

End: 1841397

Strand: Reverse

Name: fliF [H]

Synonym: VC0395_A1717

Alternate gene names: 147674591

Gene position: 1841397-1839655 (Counterclockwise)

Preceding gene: 147675704

Following gene: 147674147

Centisome position: 60.89

GC content: 50.37

Gene sequence:

>1743_bases
GTGGCCGATGATAAACAAACAACCGATCTCGCGTTGACCAATAGTGGTGGTGAGCATGCCTTGATGGCAGGCTCTGATAT
GGACCACGACACGCAGAATCCGGACATCAACGAAAAAAGTAGCAAAATGGACTTTACCGTTGGTGATCTCGATTTATTGC
GCCAAGTGGTACTGGTACTGTCTATTTCAATCTGCGTTGCCCTTATCGTCATGCTGTTCTTCTGGGTACGTGAGCCGGAT
ATGCGTCCGCTGGGTGCTTACGAAACCGAAGAACTGATCCCAGTCTTGGATTACCTCGATCAACAGAAACAGCAATACAA
ACTTGATGGCAATACCATTTTGGTGCCCGTCAGTGATTACAACAGCCTCAAACTCTCCATGGTACGCGCGGGATTAAACC
AAAACCGTCAGGCTGGCGACGAGATTCTGATGCAGGATATGGGCTTTGGGGTATCACAGCGTCTTGAACAAGAACGCCTC
AAGCTGAGCCGTGAACGTCAACTCGCGAAAGCGATTGAAGAGATGCGCCAAGTTAACAAAGCTCGCGTATTGCTTGCATT
GCCAAAGCAGAGTGTGTTTGTTCGTCATAACCAAGAAGCGTCAGCGTCTGTGTTCTTGACGGTACGCACGGGAGCCAATC
TTAAACAAGAAGAGATTGATGCCGTGGTCGATATGGTGGCCAGTGCTGTGCCGGGCATGAAACCGAGCCGGGTTACGGTG
ACCGATCAGCATGGTCGTTTGCTCAGCTCAGGCTCTCAAGATCCCGTTTCTGCAGCTCGCCGTAAAGAGCAAGAGCTGGA
GAAACAACAAGAAGAAGCCCTGCGTGGAAAAATTGACTCTGTGCTGATCCCGATCCTTGGTTTAGGTAATTACACCGCGC
AGGTGGATATCGAACTCGATTTCAGTGCAGTAGAGCAGACACGTAAAGTGTTTGACCCCAACACGCCAGCCACACGCAGT
GAATACACACTGGAAGATTACAATAACGGTAACGTTGTCGCAGGGGTGCCGGGCGCTCTTAGCAACCAACCTCCCGCCGA
TGCCTCAATTCCGCAAGATGTAGCACAGATGAAAGATGGCTCGGTCATGGGGCAAGGGTCGGTGCATAAAGAAGCGACAC
GTAACTATGAACTTGATACCACCATCAGCCATGAGCGTAAGCAATCAGGGGTGATTAACCGTCAGACTGTAGCGGTGGCC
GTCAAGAGTCGTTCCAGCGTCAATCCTGATACTGGCGAAGTCACTTACACGCCACTCAGTGAAGCGGACCTCAACTCAAT
TCGTCAGGTTCTGATCGGCACCGTAGGCTATAGTGAAAATCGTGGAGATTTACTGAATGTCCTCAGCATGCCGTTTGCTG
AACCCGAAATGGAACAAATTGTTGATGTCCCAATTTGGGAACATCCAAACTTCAACGATTGGGTTCGTTGGTTCGCCAGT
GCCTTGGTTATCATCATCGTCATTTTGGTGCTAGTACGCCCAGCGATGAAGAAACTTATCAACCCAGCGGCGGATAACGA
TGACCAAATGTACGGCCCAGACGGTATGCCAATTGGCGCGGATGGTGAGACAAGCCTCATCGGCAGCGATATTGATGGCG
GTGAGTTGTTCGAATTTGGTTCAGGCATTGATCTGCCGAATCTTCACAAGGATGAGGACGTGCTGAAAGCAGTACGTGCC
TTGGTGGCAAACGAGCCAGAACTCGCGGCTCAAGTAGTGAAGAACTGGATGCAAAATGGCTAA

Upstream 100 bases:

>100_bases
ATCGAGTGTGGCGTTTGAAGCGACCATTCAAGTACGCAATAAGCTCGTCGAAGCGTACAAAGAACTGATGAATATGCCAG
TATAACTTGGGTAATAGACT

Downstream 100 bases:

>100_bases
AGACAATAAAGATGGTGGAGAAGTGGTCGAATCAACCATTGATATCAGTGAAATTCCCGGTGAAGAAAAAGCAGCCATTT
TGCTGCTCAGTTTGAATGAA

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MADDKQTTDLALTNSGGEHALMAGSDMDHDTQNPDINEKSSKMDFTVGDLDLLRQVVLVLSISICVALIVMLFFWVREPD
MRPLGAYETEELIPVLDYLDQQKQQYKLDGNTILVPVSDYNSLKLSMVRAGLNQNRQAGDEILMQDMGFGVSQRLEQERL
KLSRERQLAKAIEEMRQVNKARVLLALPKQSVFVRHNQEASASVFLTVRTGANLKQEEIDAVVDMVASAVPGMKPSRVTV
TDQHGRLLSSGSQDPVSAARRKEQELEKQQEEALRGKIDSVLIPILGLGNYTAQVDIELDFSAVEQTRKVFDPNTPATRS
EYTLEDYNNGNVVAGVPGALSNQPPADASIPQDVAQMKDGSVMGQGSVHKEATRNYELDTTISHERKQSGVINRQTVAVA
VKSRSSVNPDTGEVTYTPLSEADLNSIRQVLIGTVGYSENRGDLLNVLSMPFAEPEMEQIVDVPIWEHPNFNDWVRWFAS
ALVIIIVILVLVRPAMKKLINPAADNDDQMYGPDGMPIGADGETSLIGSDIDGGELFEFGSGIDLPNLHKDEDVLKAVRA
LVANEPELAAQVVKNWMQNG

Sequences:

>Translated_580_residues
MADDKQTTDLALTNSGGEHALMAGSDMDHDTQNPDINEKSSKMDFTVGDLDLLRQVVLVLSISICVALIVMLFFWVREPD
MRPLGAYETEELIPVLDYLDQQKQQYKLDGNTILVPVSDYNSLKLSMVRAGLNQNRQAGDEILMQDMGFGVSQRLEQERL
KLSRERQLAKAIEEMRQVNKARVLLALPKQSVFVRHNQEASASVFLTVRTGANLKQEEIDAVVDMVASAVPGMKPSRVTV
TDQHGRLLSSGSQDPVSAARRKEQELEKQQEEALRGKIDSVLIPILGLGNYTAQVDIELDFSAVEQTRKVFDPNTPATRS
EYTLEDYNNGNVVAGVPGALSNQPPADASIPQDVAQMKDGSVMGQGSVHKEATRNYELDTTISHERKQSGVINRQTVAVA
VKSRSSVNPDTGEVTYTPLSEADLNSIRQVLIGTVGYSENRGDLLNVLSMPFAEPEMEQIVDVPIWEHPNFNDWVRWFAS
ALVIIIVILVLVRPAMKKLINPAADNDDQMYGPDGMPIGADGETSLIGSDIDGGELFEFGSGIDLPNLHKDEDVLKAVRA
LVANEPELAAQVVKNWMQNG
>Mature_579_residues
ADDKQTTDLALTNSGGEHALMAGSDMDHDTQNPDINEKSSKMDFTVGDLDLLRQVVLVLSISICVALIVMLFFWVREPDM
RPLGAYETEELIPVLDYLDQQKQQYKLDGNTILVPVSDYNSLKLSMVRAGLNQNRQAGDEILMQDMGFGVSQRLEQERLK
LSRERQLAKAIEEMRQVNKARVLLALPKQSVFVRHNQEASASVFLTVRTGANLKQEEIDAVVDMVASAVPGMKPSRVTVT
DQHGRLLSSGSQDPVSAARRKEQELEKQQEEALRGKIDSVLIPILGLGNYTAQVDIELDFSAVEQTRKVFDPNTPATRSE
YTLEDYNNGNVVAGVPGALSNQPPADASIPQDVAQMKDGSVMGQGSVHKEATRNYELDTTISHERKQSGVINRQTVAVAV
KSRSSVNPDTGEVTYTPLSEADLNSIRQVLIGTVGYSENRGDLLNVLSMPFAEPEMEQIVDVPIWEHPNFNDWVRWFASA
LVIIIVILVLVRPAMKKLINPAADNDDQMYGPDGMPIGADGETSLIGSDIDGGELFEFGSGIDLPNLHKDEDVLKAVRAL
VANEPELAAQVVKNWMQNG

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=448, Percent_Identity=33.9285714285714, Blast_Score=196, Evalue=4e-51,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 63847; Mature: 63716

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDKQTTDLALTNSGGEHALMAGSDMDHDTQNPDINEKSSKMDFTVGDLDLLRQVVLVL
CCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHH
SISICVALIVMLFFWVREPDMRPLGAYETEELIPVLDYLDQQKQQYKLDGNTILVPVSDY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEECCC
NSLKLSMVRAGLNQNRQAGDEILMQDMGFGVSQRLEQERLKLSRERQLAKAIEEMRQVNK
CCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARVLLALPKQSVFVRHNQEASASVFLTVRTGANLKQEEIDAVVDMVASAVPGMKPSRVTV
HEEEEEECCCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
TDQHGRLLSSGSQDPVSAARRKEQELEKQQEEALRGKIDSVLIPILGLGNYTAQVDIELD
ECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
FSAVEQTRKVFDPNTPATRSEYTLEDYNNGNVVAGVPGALSNQPPADASIPQDVAQMKDG
HHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCCC
SVMGQGSVHKEATRNYELDTTISHERKQSGVINRQTVAVAVKSRSSVNPDTGEVTYTPLS
CEECCCCCCHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCCC
EADLNSIRQVLIGTVGYSENRGDLLNVLSMPFAEPEMEQIVDVPIWEHPNFNDWVRWFAS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHH
ALVIIIVILVLVRPAMKKLINPAADNDDQMYGPDGMPIGADGETSLIGSDIDGGELFEFG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHCC
SGIDLPNLHKDEDVLKAVRALVANEPELAAQVVKNWMQNG
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADDKQTTDLALTNSGGEHALMAGSDMDHDTQNPDINEKSSKMDFTVGDLDLLRQVVLVL
CCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHH
SISICVALIVMLFFWVREPDMRPLGAYETEELIPVLDYLDQQKQQYKLDGNTILVPVSDY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEECCC
NSLKLSMVRAGLNQNRQAGDEILMQDMGFGVSQRLEQERLKLSRERQLAKAIEEMRQVNK
CCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARVLLALPKQSVFVRHNQEASASVFLTVRTGANLKQEEIDAVVDMVASAVPGMKPSRVTV
HEEEEEECCCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
TDQHGRLLSSGSQDPVSAARRKEQELEKQQEEALRGKIDSVLIPILGLGNYTAQVDIELD
ECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
FSAVEQTRKVFDPNTPATRSEYTLEDYNNGNVVAGVPGALSNQPPADASIPQDVAQMKDG
HHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCCC
SVMGQGSVHKEATRNYELDTTISHERKQSGVINRQTVAVAVKSRSSVNPDTGEVTYTPLS
CEECCCCCCHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCCC
EADLNSIRQVLIGTVGYSENRGDLLNVLSMPFAEPEMEQIVDVPIWEHPNFNDWVRWFAS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHH
ALVIIIVILVLVRPAMKKLINPAADNDDQMYGPDGMPIGADGETSLIGSDIDGGELFEFG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHCC
SGIDLPNLHKDEDVLKAVRALVANEPELAAQVVKNWMQNG
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8675317; 10984043 [H]