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The map label for this gene is yieO [C]

Identifier: 147674445

GI number: 147674445

Start: 1263369

End: 1264802

Strand: Reverse

Name: yieO [C]

Synonym: VC0395_A1199

Alternate gene names: 147674445

Gene position: 1264802-1263369 (Counterclockwise)

Preceding gene: 147674541

Following gene: 147674295

Centisome position: 41.82

GC content: 49.02

Gene sequence:

>1434_bases
ATGTCGCAGCCTGTAGATAGAAAAACCTTTTTGGGCATCTTTTTTACGGTTTTTTTGCCAATGTTTTTGGCGGCCGTTGA
TCAAACCTTGCTGGCTACCGCGACGCCTGCGATTGTGCGCGATCTCGGCAACTTGCAACTTTCTTCGTGGATTGCGATTG
GTTACATGCTGGCAAGTGCAGCCAGTGTTCCGGTTTACGGCTGGTTAGGGGATCAGTATGGACGTAAACCGATGTTAATG
ATTGCGCTGAGTATTTTTGCCTTAGGTTCCGTCATTTGTGCTTTTGCAATGAATATGCCGTGGTTGGTGGCTGGTCGGAT
TGTACAAGGACTGGGTAGCGGCGGCTTAATGAGCTTATCACAAGCCTTGATCGGGGAATTAGTACCGCCCCGCGAGCGTG
CCCGTTTTCAGGGCTATTTTTCCTCACTTTTTGCGGTGGCCAGTATTGGGGGGCCAGTGTTAGGTGGTGTATTGGTAACC
TACCTTTCTTGGCAATGGCTGTTTTGGTTTAACCTGCCGCTTGTGGGCATCGCTTTGTATCGACTGGCACGATTTCAATT
TGTGCAAAGTTCGCGTAAGTCTGAGGCTTCAATTGATGTGGCTGGGCTTATCCTGTTTCCCAGTATGATGACTCTCATCA
TCTATTGGCTTTCGGCTGGCGGGCACTATTTTCCGTGGTTCTCGGTCACCAGCATAAGCCTAGTGAGTGCATTTGTTTTA
ACGAGCTTGCTGTTTATCGCCCAGCAAAAGCGAGTCACGGCTTCCTTTTTACCACTCCGTTTATTGGCGCGTCGTGAAAT
ACATATTCCGTTGCTCTCCACGTTCATTTTCGCGGCTTGTTTGTTTGCGCTGATCTTCTTTTTACCGATTTTTTTACAGC
TCGGCTTACGAGTTAACGCAGCACAATCGGGACTACTGCTTATCCCCCTGTCATGCGGCATTATTGGTGGCGCCTATACG
ACCGGTAAAGTGATTGCTAAAACAGGAGTGCCCAAATTTTTGCCTGTGTTTGGTATGAGTTTAAGTAGCTGCGCCTTTGC
TGTGTTGGCGATGTTTTCACTCTCTGCGCCCGTGGTGAGTGCGATTGCTGCCTTATGTGGTTTAGGCTTAGGCACTGTGA
TGCCCTCAACCCAAGTTTTAGTTCAAACATTGGCAGGCAAAGCCAATTTAGGGCGGATCACCGCGATGGCTTCACTGAGC
CGCTCGTTAGGGGCATCGGTCGGCACCGCTTTCTTTGGTACATTGATTTACTCCTTACTTCCCGGTTTATCGCCAAACAG
TGGTTTGCAGGCGATTGCCGCATTGCCACAAAGTGAAATTTTGCATGCTTTTCAAATTGGCTTTGCGGTTGCGGCGCTGC
TTGCGTTGCTAGGGGCATTGAATGCGTTGCGCGCTCCGAAAATTCAGTTGGATGATTACGCCTTTGACTCTTAA

Upstream 100 bases:

>100_bases
TAAGGAGTAAGCTTGAGCGCGAGATGTCCACATCATGCTGATTTTATGAATATCTTAAGTGGATGTTGTCGTGTTCTAGT
GAGTTATCGATGAGGAAGGT

Downstream 100 bases:

>100_bases
ATATCACTATACCTAAACGATTTGGAGTTGCAGGTGCAGTAACCAGATTGACTCGCCCTGTACGCCTTGTGTAAACGTTT
AACTTTGATGCTTGAAGAGA

Product: putative multidrug transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSQPVDRKTFLGIFFTVFLPMFLAAVDQTLLATATPAIVRDLGNLQLSSWIAIGYMLASAASVPVYGWLGDQYGRKPMLM
IALSIFALGSVICAFAMNMPWLVAGRIVQGLGSGGLMSLSQALIGELVPPRERARFQGYFSSLFAVASIGGPVLGGVLVT
YLSWQWLFWFNLPLVGIALYRLARFQFVQSSRKSEASIDVAGLILFPSMMTLIIYWLSAGGHYFPWFSVTSISLVSAFVL
TSLLFIAQQKRVTASFLPLRLLARREIHIPLLSTFIFAACLFALIFFLPIFLQLGLRVNAAQSGLLLIPLSCGIIGGAYT
TGKVIAKTGVPKFLPVFGMSLSSCAFAVLAMFSLSAPVVSAIAALCGLGLGTVMPSTQVLVQTLAGKANLGRITAMASLS
RSLGASVGTAFFGTLIYSLLPGLSPNSGLQAIAALPQSEILHAFQIGFAVAALLALLGALNALRAPKIQLDDYAFDS

Sequences:

>Translated_477_residues
MSQPVDRKTFLGIFFTVFLPMFLAAVDQTLLATATPAIVRDLGNLQLSSWIAIGYMLASAASVPVYGWLGDQYGRKPMLM
IALSIFALGSVICAFAMNMPWLVAGRIVQGLGSGGLMSLSQALIGELVPPRERARFQGYFSSLFAVASIGGPVLGGVLVT
YLSWQWLFWFNLPLVGIALYRLARFQFVQSSRKSEASIDVAGLILFPSMMTLIIYWLSAGGHYFPWFSVTSISLVSAFVL
TSLLFIAQQKRVTASFLPLRLLARREIHIPLLSTFIFAACLFALIFFLPIFLQLGLRVNAAQSGLLLIPLSCGIIGGAYT
TGKVIAKTGVPKFLPVFGMSLSSCAFAVLAMFSLSAPVVSAIAALCGLGLGTVMPSTQVLVQTLAGKANLGRITAMASLS
RSLGASVGTAFFGTLIYSLLPGLSPNSGLQAIAALPQSEILHAFQIGFAVAALLALLGALNALRAPKIQLDDYAFDS
>Mature_476_residues
SQPVDRKTFLGIFFTVFLPMFLAAVDQTLLATATPAIVRDLGNLQLSSWIAIGYMLASAASVPVYGWLGDQYGRKPMLMI
ALSIFALGSVICAFAMNMPWLVAGRIVQGLGSGGLMSLSQALIGELVPPRERARFQGYFSSLFAVASIGGPVLGGVLVTY
LSWQWLFWFNLPLVGIALYRLARFQFVQSSRKSEASIDVAGLILFPSMMTLIIYWLSAGGHYFPWFSVTSISLVSAFVLT
SLLFIAQQKRVTASFLPLRLLARREIHIPLLSTFIFAACLFALIFFLPIFLQLGLRVNAAQSGLLLIPLSCGIIGGAYTT
GKVIAKTGVPKFLPVFGMSLSSCAFAVLAMFSLSAPVVSAIAALCGLGLGTVMPSTQVLVQTLAGKANLGRITAMASLSR
SLGASVGTAFFGTLIYSLLPGLSPNSGLQAIAALPQSEILHAFQIGFAVAALLALLGALNALRAPKIQLDDYAFDS

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=371, Percent_Identity=29.1105121293801, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=29.1566265060241, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789042, Length=318, Percent_Identity=27.3584905660377, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788392, Length=335, Percent_Identity=27.1641791044776, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788710, Length=296, Percent_Identity=28.0405405405405, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790146, Length=180, Percent_Identity=28.3333333333333, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI48994889, Length=109, Percent_Identity=35.7798165137615, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=354, Percent_Identity=26.271186440678, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322958, Length=444, Percent_Identity=25.6756756756757, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=33.5526315789474, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325455, Length=273, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6319770, Length=413, Percent_Identity=25.90799031477, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319783, Length=366, Percent_Identity=24.8633879781421, Blast_Score=83, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50804; Mature: 50673

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
>Mature Secondary Structure 
SQPVDRKTFLGIFFTVFLPMFLAAVDQTLLATATPAIVRDLGNLQLSSWIAIGYMLASA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHH
ASVPVYGWLGDQYGRKPMLMIALSIFALGSVICAFAMNMPWLVAGRIVQGLGSGGLMSLS
CCCCEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHH
QALIGELVPPRERARFQGYFSSLFAVASIGGPVLGGVLVTYLSWQWLFWFNLPLVGIALY
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLARFQFVQSSRKSEASIDVAGLILFPSMMTLIIYWLSAGGHYFPWFSVTSISLVSAFVL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
TSLLFIAQQKRVTASFLPLRLLARREIHIPLLSTFIFAACLFALIFFLPIFLQLGLRVNA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEC
AQSGLLLIPLSCGIIGGAYTTGKVIAKTGVPKFLPVFGMSLSSCAFAVLAMFSLSAPVVS
CCCCEEEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHCHHHHH
AIAALCGLGLGTVMPSTQVLVQTLAGKANLGRITAMASLSRSLGASVGTAFFGTLIYSLL
HHHHHHHCCHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGLSPNSGLQAIAALPQSEILHAFQIGFAVAALLALLGALNALRAPKIQLDDYAFDS
CCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]