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The map label for this gene is rpfG [H]

Identifier: 147674364

GI number: 147674364

Start: 993822

End: 995147

Strand: Reverse

Name: rpfG [H]

Synonym: VC0395_A0963

Alternate gene names: 147674364

Gene position: 995147-993822 (Counterclockwise)

Preceding gene: 147674520

Following gene: 147673074

Centisome position: 32.91

GC content: 45.85

Gene sequence:

>1326_bases
ATGGCAACCGCCAATATCGCCATCATGCAAAACAGTAGTACGAACATCGCTTTTATCGAGCGCATGCCATCTCCTTCTCT
ACACAGGATTTTGTATAACCTATTTTCAAAGTTTAGCTATAGTTTAAGCAACTCATGTCACTTTAATCTTAAATGCCTAA
ACTTTGTGATGAATAGGATGTTTCACATGTCAATGGAAGACCTTAGCCAATGCACGATACTGATCGTCGATGACTCGCCA
GACAATATCGCCTTTATGTCGCAAGGGCTGGCACAGTACTATCGAATCAAGGCTGCTCGCTCAGGAAAAGTGGCACTCGA
GATTTTGGCGCAATACCCTATCGATCTGGTTTTACTCGATATTGTCATGCCAGAAATGTCGGGCTATGAGGTAATTAATC
AAATCAAACATAACCCGCATACCGAGCATATTCCGGTGATTTTTCTTACAGGTAAAAGTAATCCCGAAGATGAGCAATTA
GGCTTTGAGCTTGGTGCCGTTGATTATGTTTTTAAACCGGTCAGCATTCCGCTGCTGAAATCGCGCGTTCACACTCACTT
ACAAAATAAGCGCTCTAAAGACATTTTATTGAATCAAAACGATTATCTGGAAACCGAAGTGCTGCGACGGTCCGGAGAAT
TGGATCGCATGCAAGATGCTGTGGTGTTTGCGCTGGCCAGTCTTGCAGAAACACGCGATCCGGAGACAGGTAATCACCTA
CTGCGTACCCAACATTACGTGAAAGTATTAGCCCAGCGTCTCGCCACCACGGACAAATACCGCGATGTACTGAGCCCGAC
CGTGATTGATACTTACTTTAAAGCGGCACCGTTGCACGACATAGGTAAAGTCGGCATTCCCGACAATATTCTGCTCAAAC
CGGGCAAATTGACCCCAGATGAGTTTACCACTATGCGCAATCACGCCTTGCTGGGTAAGCTTGCTCTCGAAAAAGCGGAG
AAACTTTCCGGAGCCTGTACCGCGCTCATCAATGTCGCAAAAGAAATTGCCATGGGTCATCATGAAAAGTGGGACGGTTC
AGGTTATCCACTAGGACTCAAAGGCGACGATATTCCTCTGAGTGCCCGCTTGATGGCTCTCGCCGATGTGTATGACGCTT
TGATTTGCCGCCGCGTTTATAAAGAACCGATGAGTCATGAAGAGGCCAAAGCGATCATTTTACAAGGCCGAGGTTCACAT
TTTGACCCTATGGTGATTGATGCCTTTTTAATAGAAGAGCAGAACTTCATCGACATTGCACAAAAGTTTGCCGATGAAGA
ATCTGCTATGGTACCGATTCAACTGGGTCAACAAGCGTCAGGCTGA

Upstream 100 bases:

>100_bases
TTGTTTTAACTCTTGAGATAGACGATAAAAGTGAAAAAGCTGCTCGCTGTATTGATGGCTCTCTAGCCTAAGATCTGCGA
CTTGCCGAGTCACTTTAAAC

Downstream 100 bases:

>100_bases
TTTTCTGGAGCAGCCTGCGCAAACGCAGGCAAAGCACGTAACCTTGCTGCGATCTGCTGTAAGGTGGGATAACGACTCAT
ATCGAGATCAAATCGCTCAG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MATANIAIMQNSSTNIAFIERMPSPSLHRILYNLFSKFSYSLSNSCHFNLKCLNFVMNRMFHMSMEDLSQCTILIVDDSP
DNIAFMSQGLAQYYRIKAARSGKVALEILAQYPIDLVLLDIVMPEMSGYEVINQIKHNPHTEHIPVIFLTGKSNPEDEQL
GFELGAVDYVFKPVSIPLLKSRVHTHLQNKRSKDILLNQNDYLETEVLRRSGELDRMQDAVVFALASLAETRDPETGNHL
LRTQHYVKVLAQRLATTDKYRDVLSPTVIDTYFKAAPLHDIGKVGIPDNILLKPGKLTPDEFTTMRNHALLGKLALEKAE
KLSGACTALINVAKEIAMGHHEKWDGSGYPLGLKGDDIPLSARLMALADVYDALICRRVYKEPMSHEEAKAIILQGRGSH
FDPMVIDAFLIEEQNFIDIAQKFADEESAMVPIQLGQQASG

Sequences:

>Translated_441_residues
MATANIAIMQNSSTNIAFIERMPSPSLHRILYNLFSKFSYSLSNSCHFNLKCLNFVMNRMFHMSMEDLSQCTILIVDDSP
DNIAFMSQGLAQYYRIKAARSGKVALEILAQYPIDLVLLDIVMPEMSGYEVINQIKHNPHTEHIPVIFLTGKSNPEDEQL
GFELGAVDYVFKPVSIPLLKSRVHTHLQNKRSKDILLNQNDYLETEVLRRSGELDRMQDAVVFALASLAETRDPETGNHL
LRTQHYVKVLAQRLATTDKYRDVLSPTVIDTYFKAAPLHDIGKVGIPDNILLKPGKLTPDEFTTMRNHALLGKLALEKAE
KLSGACTALINVAKEIAMGHHEKWDGSGYPLGLKGDDIPLSARLMALADVYDALICRRVYKEPMSHEEAKAIILQGRGSH
FDPMVIDAFLIEEQNFIDIAQKFADEESAMVPIQLGQQASG
>Mature_440_residues
ATANIAIMQNSSTNIAFIERMPSPSLHRILYNLFSKFSYSLSNSCHFNLKCLNFVMNRMFHMSMEDLSQCTILIVDDSPD
NIAFMSQGLAQYYRIKAARSGKVALEILAQYPIDLVLLDIVMPEMSGYEVINQIKHNPHTEHIPVIFLTGKSNPEDEQLG
FELGAVDYVFKPVSIPLLKSRVHTHLQNKRSKDILLNQNDYLETEVLRRSGELDRMQDAVVFALASLAETRDPETGNHLL
RTQHYVKVLAQRLATTDKYRDVLSPTVIDTYFKAAPLHDIGKVGIPDNILLKPGKLTPDEFTTMRNHALLGKLALEKAEK
LSGACTALINVAKEIAMGHHEKWDGSGYPLGLKGDDIPLSARLMALADVYDALICRRVYKEPMSHEEAKAIILQGRGSHF
DPMVIDAFLIEEQNFIDIAQKFADEESAMVPIQLGQQASG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=31.3559322033898, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=205, Percent_Identity=25.8536585365854, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788550, Length=184, Percent_Identity=31.5217391304348, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI145693157, Length=124, Percent_Identity=33.0645161290323, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790437, Length=121, Percent_Identity=35.5371900826446, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 49426; Mature: 49294

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATANIAIMQNSSTNIAFIERMPSPSLHRILYNLFSKFSYSLSNSCHFNLKCLNFVMNRM
CCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
FHMSMEDLSQCTILIVDDSPDNIAFMSQGLAQYYRIKAARSGKVALEILAQYPIDLVLLD
HHCCHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHH
IVMPEMSGYEVINQIKHNPHTEHIPVIFLTGKSNPEDEQLGFELGAVDYVFKPVSIPLLK
HHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHCCCCHHHHH
SRVHTHLQNKRSKDILLNQNDYLETEVLRRSGELDRMQDAVVFALASLAETRDPETGNHL
HHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
LRTQHYVKVLAQRLATTDKYRDVLSPTVIDTYFKAAPLHDIGKVGIPDNILLKPGKLTPD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHCCCCCCCCCEEECCCCCCHH
EFTTMRNHALLGKLALEKAEKLSGACTALINVAKEIAMGHHEKWDGSGYPLGLKGDDIPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
SARLMALADVYDALICRRVYKEPMSHEEAKAIILQGRGSHFDPMVIDAFLIEEQNFIDIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHH
QKFADEESAMVPIQLGQQASG
HHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
ATANIAIMQNSSTNIAFIERMPSPSLHRILYNLFSKFSYSLSNSCHFNLKCLNFVMNRM
CCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
FHMSMEDLSQCTILIVDDSPDNIAFMSQGLAQYYRIKAARSGKVALEILAQYPIDLVLLD
HHCCHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHH
IVMPEMSGYEVINQIKHNPHTEHIPVIFLTGKSNPEDEQLGFELGAVDYVFKPVSIPLLK
HHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHCCCCHHHHH
SRVHTHLQNKRSKDILLNQNDYLETEVLRRSGELDRMQDAVVFALASLAETRDPETGNHL
HHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
LRTQHYVKVLAQRLATTDKYRDVLSPTVIDTYFKAAPLHDIGKVGIPDNILLKPGKLTPD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHCCCCCCCCCEEECCCCCCHH
EFTTMRNHALLGKLALEKAEKLSGACTALINVAKEIAMGHHEKWDGSGYPLGLKGDDIPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
SARLMALADVYDALICRRVYKEPMSHEEAKAIILQGRGSHFDPMVIDAFLIEEQNFIDIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHH
QKFADEESAMVPIQLGQQASG
HHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]