Click here to switch to the map view.

The map label for this gene is rpoA

Identifier: 147674278

GI number: 147674278

Start: 2305405

End: 2306397

Strand: Reverse

Name: rpoA

Synonym: VC0395_A2149

Alternate gene names: 147674278

Gene position: 2306397-2305405 (Counterclockwise)

Preceding gene: 147675276

Following gene: 147674916

Centisome position: 76.27

GC content: 47.03

Gene sequence:

>993_bases
ATGCAGGGTTCTGTAACAGAATTTCTTAAGCCACGTCTTGTTGATATCGAACAAATCAGCACGACACACGCAAAAGTAAC
TCTTGAGCCGTTAGAGCGTGGTTTCGGCCATACTCTGGGTAATGCACTTCGCCGTATTCTTCTATCTTCAATGCCAGGTT
GTGCTGTGACTGAAGTAGAGATTGAAGGCGTTCTTCACGAGTACAGCACCAAAGAAGGTGTTCAGGAAGATATCCTTGAG
ATTCTCTTGAACCTGAAAGGTCTGGCTGTTCGCGTTGCCGAAGGCAAAGATGAAGTGTTCATTACACTGAACAAATCAGG
CTCGGGCCCTGTGGTTGCAGGTGACATCACCCATGACGGTGATGTAGAGATCGTAAACCCTGAACACGTTATTTGTCATT
TAACTTCTGACAATGCTGCGATCGCTATGCGTATCAAAGTAGAACGTGGTCGTGGTTATGTTCCAGCTTCTGCCCGTATC
CATACTGAAGAAGATGAGCGTCCAATTGGTCGTTTGCTTGTTGACGCGACTTTCAGCCCAGTAGACAAAATTGCCTACTC
TGTTGAAGCAGCTCGTGTTGAACAGCGTACTGACTTGGACAAGCTTGTTATCGATATGGAAACTAACGGTACTCTTGAGC
CTGAGGAAGCAATCCGTCGCGCAGCAACAATTCTTGCTGAGCAATTGGATGCGTTCGTAGATCTTCGTGATGTACGTGTA
CCTGAGGAGAAGGAAGAGAAGCCAGAATTCGATCCGATCCTACTGCGTCCTGTAGACGATCTTGAACTAACAGTTCGCTC
TGCTAACTGTCTGAAAGCAGAAGCGATTCACTACATCGGTGATCTGGTACAGCGCACTGAGGTTGAGCTTCTTAAAACGC
CAAACCTCGGTAAGAAGTCTCTTACAGAGATTAAAGACGTGCTTGCATCACGTGGTCTGTCTCTGGGCATGCGTCTAGAA
AACTGGCCACCAGCGTCAATCGCTGAAGATTAA

Upstream 100 bases:

>100_bases
CAAGCGTATGCCTGAGCGTTCCGATCTGTCTGCTGACATCAACGAACAATTGATCGTCGAGCTTTACTCTAAGTAAGGTT
TAAACTAAAGAGAGGACACA

Downstream 100 bases:

>100_bases
TCGATATTAGTTAGAAGGATTAGGTCATGCGCCATCGTAAGAGTGGTCGTCAACTCAACCGCAACAGCAGTCATCGCAAA
GCGATGTTCAGCAACATGGC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MQGSVTEFLKPRLVDIEQISTTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIEGVLHEYSTKEGVQEDILE
ILLNLKGLAVRVAEGKDEVFITLNKSGSGPVVAGDITHDGDVEIVNPEHVICHLTSDNAAIAMRIKVERGRGYVPASARI
HTEEDERPIGRLLVDATFSPVDKIAYSVEAARVEQRTDLDKLVIDMETNGTLEPEEAIRRAATILAEQLDAFVDLRDVRV
PEEKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLE
NWPPASIAED

Sequences:

>Translated_330_residues
MQGSVTEFLKPRLVDIEQISTTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIEGVLHEYSTKEGVQEDILE
ILLNLKGLAVRVAEGKDEVFITLNKSGSGPVVAGDITHDGDVEIVNPEHVICHLTSDNAAIAMRIKVERGRGYVPASARI
HTEEDERPIGRLLVDATFSPVDKIAYSVEAARVEQRTDLDKLVIDMETNGTLEPEEAIRRAATILAEQLDAFVDLRDVRV
PEEKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLE
NWPPASIAED
>Mature_330_residues
MQGSVTEFLKPRLVDIEQISTTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIEGVLHEYSTKEGVQEDILE
ILLNLKGLAVRVAEGKDEVFITLNKSGSGPVVAGDITHDGDVEIVNPEHVICHLTSDNAAIAMRIKVERGRGYVPASARI
HTEEDERPIGRLLVDATFSPVDKIAYSVEAARVEQRTDLDKLVIDMETNGTLEPEEAIRRAATILAEQLDAFVDLRDVRV
PEEKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLE
NWPPASIAED

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=330, Percent_Identity=90.3030303030303, Blast_Score=606, Evalue=1e-175,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_VIBC3 (A5F572)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F572
- STRING:   A5F572
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2149
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   VCHO345073:VC0395_A2149-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 36416; Mature: 36416

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGSVTEFLKPRLVDIEQISTTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
CCCCHHHHHCCCCCCHHHHCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEE
IEGVLHEYSTKEGVQEDILEILLNLKGLAVRVAEGKDEVFITLNKSGSGPVVAGDITHDG
HHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCEEECCCCCCC
DVEIVNPEHVICHLTSDNAAIAMRIKVERGRGYVPASARIHTEEDERPIGRLLVDATFSP
CEEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCEEECCCCCCCHHHEEEECCCCH
VDKIAYSVEAARVEQRTDLDKLVIDMETNGTLEPEEAIRRAATILAEQLDAFVDLRDVRV
HHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEEKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKS
CCCCCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LTEIKDVLASRGLSLGMRLENWPPASIAED
HHHHHHHHHHCCCEECCEECCCCCCCCCCC
>Mature Secondary Structure
MQGSVTEFLKPRLVDIEQISTTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
CCCCHHHHHCCCCCCHHHHCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEE
IEGVLHEYSTKEGVQEDILEILLNLKGLAVRVAEGKDEVFITLNKSGSGPVVAGDITHDG
HHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCEEECCCCCCC
DVEIVNPEHVICHLTSDNAAIAMRIKVERGRGYVPASARIHTEEDERPIGRLLVDATFSP
CEEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCEEECCCCCCCHHHEEEECCCCH
VDKIAYSVEAARVEQRTDLDKLVIDMETNGTLEPEEAIRRAATILAEQLDAFVDLRDVRV
HHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEEKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKS
CCCCCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LTEIKDVLASRGLSLGMRLENWPPASIAED
HHHHHHHHHHCCCEECCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA