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The map label for this gene is nhaA

Identifier: 147674219

GI number: 147674219

Start: 1302441

End: 1303589

Strand: Reverse

Name: nhaA

Synonym: VC0395_A1233

Alternate gene names: 147674219

Gene position: 1303589-1302441 (Counterclockwise)

Preceding gene: 147674640

Following gene: 147674813

Centisome position: 43.11

GC content: 47.43

Gene sequence:

>1149_bases
ATGTCTGACATGATTCGAGATTTCTTCAAGATGGAATCTGCGGGGGGCATTCTGCTGGTGATTGCGGCGGCGATTGCTAT
GGTCATTGCCAATTCAGCAATGGGTGAAGGGTACCAAGCATTTTTACATACCTATGTCTTTGGTATGTCGGTTTCACACT
GGATTAATGATGGCCTAATGGCGGTGTTTTTCTTATTGATCGGTTTAGAGGTCAAACGTGAACTGCTGGAAGGAGCGCTG
AAATCCCGTGAAACAGCGATTTTCCCTGCGATAGCGGCGGTAGGCGGTATGCTCGCTCCTGCATTAATCTATGTAGCCTT
CAATTTTAACGATCCGGCTGCCATTCAAGGTTGGGCGATTCCTGCGGCCACCGATATTGCGTTTGCGCTCGGGATCATGG
CGCTACTGGGTAAGCGTGTGCCTGTGAGTTTGAAAGTGTTTCTGCTGGCGCTGGCGATCATTGATGACCTTGGCGTAGTA
GTGATCATCGCTCTGTTTTACAGCAGTGATTTATCAACCATTGCACTCACTATCGGCTTTATCATGACGGGTGTGCTGTT
TATGCTTAATGCCAAGCACGTTACTAAATTGAGTATTTATTTGGTGGCAGGCCTCATCCTATGGATCGCCGTATTAAAGT
CGGGTGTCCACGCCACATTGGCTGGAGTAGTGATCGGATTTGCTATTCCGCTGAAAGGCAATAAGGGTGAGCATTCACCG
TTGAAACACCTTGAGCATGCACTGCATCCTTATGTTGCCTTTGCGATTTTACCTGTGTTTGCTTTTGCCAATGCTGGTAT
CTCACTGCAAGGCGTGTCATTAGCAGGGCTTACTTCTATGCTGCCACTGGGTGTAGCACTGGGTCTGTTCCTTGGTAAAC
CACTCGGCATCTTTAGCTTCAGTTGGGCTGCGGTTAAACTCGGGGTAGCAAAACTGCCAGAAGGGATCAACTTCAAGCAT
ATTTTTGCAGTATCGGTACTGTGTGGCATCGGTTTTACTATGTCGATCTTCATTTCATCACTGGCCTTTGGTCAAGCGAA
TGAAGCTTATGATACTTATGCGCGCTTAGGTATTTTAATGGGGTCAACCACCGCGGCATTACTTGGTTATTCTTTACTAC
GACTCTCTCTGCCGCTCAAAAAAGCGTAA

Upstream 100 bases:

>100_bases
AAATCCTCATTACAATCGATTTTTCCATTACACTAAGCCTAGCTACACTCTGGAACATTCTTTTGTTAATCAATTATTAA
CATAATTTAAGGACAAACCT

Downstream 100 bases:

>100_bases
TCATTTCATCAGGCTTTGAACAATTCTAGCCTCCGCAATGCGGGGGCTTTTTTGTAGTTTCACACTATGGCAGTGTGCTG
AGTTTGCAACTCAATTGAGA

Product: pH-dependent sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSDMIRDFFKMESAGGILLVIAAAIAMVIANSAMGEGYQAFLHTYVFGMSVSHWINDGLMAVFFLLIGLEVKRELLEGAL
KSRETAIFPAIAAVGGMLAPALIYVAFNFNDPAAIQGWAIPAATDIAFALGIMALLGKRVPVSLKVFLLALAIIDDLGVV
VIIALFYSSDLSTIALTIGFIMTGVLFMLNAKHVTKLSIYLVAGLILWIAVLKSGVHATLAGVVIGFAIPLKGNKGEHSP
LKHLEHALHPYVAFAILPVFAFANAGISLQGVSLAGLTSMLPLGVALGLFLGKPLGIFSFSWAAVKLGVAKLPEGINFKH
IFAVSVLCGIGFTMSIFISSLAFGQANEAYDTYARLGILMGSTTAALLGYSLLRLSLPLKKA

Sequences:

>Translated_382_residues
MSDMIRDFFKMESAGGILLVIAAAIAMVIANSAMGEGYQAFLHTYVFGMSVSHWINDGLMAVFFLLIGLEVKRELLEGAL
KSRETAIFPAIAAVGGMLAPALIYVAFNFNDPAAIQGWAIPAATDIAFALGIMALLGKRVPVSLKVFLLALAIIDDLGVV
VIIALFYSSDLSTIALTIGFIMTGVLFMLNAKHVTKLSIYLVAGLILWIAVLKSGVHATLAGVVIGFAIPLKGNKGEHSP
LKHLEHALHPYVAFAILPVFAFANAGISLQGVSLAGLTSMLPLGVALGLFLGKPLGIFSFSWAAVKLGVAKLPEGINFKH
IFAVSVLCGIGFTMSIFISSLAFGQANEAYDTYARLGILMGSTTAALLGYSLLRLSLPLKKA
>Mature_381_residues
SDMIRDFFKMESAGGILLVIAAAIAMVIANSAMGEGYQAFLHTYVFGMSVSHWINDGLMAVFFLLIGLEVKRELLEGALK
SRETAIFPAIAAVGGMLAPALIYVAFNFNDPAAIQGWAIPAATDIAFALGIMALLGKRVPVSLKVFLLALAIIDDLGVVV
IIALFYSSDLSTIALTIGFIMTGVLFMLNAKHVTKLSIYLVAGLILWIAVLKSGVHATLAGVVIGFAIPLKGNKGEHSPL
KHLEHALHPYVAFAILPVFAFANAGISLQGVSLAGLTSMLPLGVALGLFLGKPLGIFSFSWAAVKLGVAKLPEGINFKHI
FAVSVLCGIGFTMSIFISSLAFGQANEAYDTYARLGILMGSTTAALLGYSLLRLSLPLKKA

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=382, Percent_Identity=57.5916230366492, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA_VIBC3 (A5F7R1)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F7R1
- SMR:   A5F7R1
- STRING:   A5F7R1
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1233
- eggNOG:   COG3004
- HOGENOM:   HBG665619
- OMA:   DLWINDG
- ProtClustDB:   PRK09560
- BioCyc:   VCHO345073:VC0395_A1233-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 40317; Mature: 40186

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x19bd4114)-; HASH(0x19ff3158)-; HASH(0x19ff5468)-; HASH(0x19ff311c)-; HASH(0x16e68da0)-; HASH(0x18ecd814)-; HASH(0x19e4a2b0)-; HASH(0x19e4a238)-; HASH(0x16e688b4)-; HASH(0x1957f780)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMIRDFFKMESAGGILLVIAAAIAMVIANSAMGEGYQAFLHTYVFGMSVSHWINDGLM
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHH
AVFFLLIGLEVKRELLEGALKSRETAIFPAIAAVGGMLAPALIYVAFNFNDPAAIQGWAI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
PAATDIAFALGIMALLGKRVPVSLKVFLLALAIIDDLGVVVIIALFYSSDLSTIALTIGF
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IMTGVLFMLNAKHVTKLSIYLVAGLILWIAVLKSGVHATLAGVVIGFAIPLKGNKGEHSP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCCCCCH
LKHLEHALHPYVAFAILPVFAFANAGISLQGVSLAGLTSMLPLGVALGLFLGKPLGIFSF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
SWAAVKLGVAKLPEGINFKHIFAVSVLCGIGFTMSIFISSLAFGQANEAYDTYARLGILM
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GSTTAALLGYSLLRLSLPLKKA
CCHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure 
SDMIRDFFKMESAGGILLVIAAAIAMVIANSAMGEGYQAFLHTYVFGMSVSHWINDGLM
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHH
AVFFLLIGLEVKRELLEGALKSRETAIFPAIAAVGGMLAPALIYVAFNFNDPAAIQGWAI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
PAATDIAFALGIMALLGKRVPVSLKVFLLALAIIDDLGVVVIIALFYSSDLSTIALTIGF
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IMTGVLFMLNAKHVTKLSIYLVAGLILWIAVLKSGVHATLAGVVIGFAIPLKGNKGEHSP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCCCCCH
LKHLEHALHPYVAFAILPVFAFANAGISLQGVSLAGLTSMLPLGVALGLFLGKPLGIFSF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
SWAAVKLGVAKLPEGINFKHIFAVSVLCGIGFTMSIFISSLAFGQANEAYDTYARLGILM
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GSTTAALLGYSLLRLSLPLKKA
CCHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA