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The map label for this gene is lysC [H]

Identifier: 147674175

GI number: 147674175

Start: 70096

End: 71283

Strand: Direct

Name: lysC [H]

Synonym: VC0395_A0074

Alternate gene names: 147674175

Gene position: 70096-71283 (Clockwise)

Preceding gene: 147673460

Following gene: 147674619

Centisome position: 2.32

GC content: 47.64

Gene sequence:

>1188_bases
GTGAAAAAGCCCCTTATCGTGCAAAAGTTCGGCGGAACTTCGGTGGGTTCAATAGAAAGAATGCAAACCGTAGCCGAACA
CATCATTAAGGCGAAGAATGATGGCAATCAAGTTGTCGTAGTGGTCTCTGCCATGGCAGGGGAAACCAATCGACTGGTGG
GTTTAGCTCAACAAGTAGATAGCGTACCAAACGCTAGAGAACTTGATGTTTTGCTCTCTGCTGGTGAGCAGGTGTCAATG
GCCTTAGTGGCAATGACACTGCACAAAATGGGCTATGCCGCCCGCTCACTAACCGGAGCTCAAGCGAATATTGTGACGGA
CAATCAACACAATGACGCGACGATTAAACATATTGATACTCGAACGATTACCGAGTTGCTAGAACAAGATTTTATTGTGA
TAGTCGCGGGTTTTCAGGGCATTAATGAAAATGGGGATATCACTACGTTAGGCCGTGGTGGCTCAGACACCAGTGCTGTG
GCATTGGCTGGGGCACTGAATGCCGACGAATGCCAAATATTCACTGATGTCGATGGGATATACACTTGTGATCCACGCAT
TGTACCAAGTGCTCGTAAGCTGGATGTGATTGATTTTCCTTCCATGGAAGAGATGGCGCGCAAAGGTGCAAAAGTGTTGC
ACCTACCGTGCGTGCAGTATGCTTGGAAGCATGCAGTGCCACTCAGAGTGCTCTCTACCTTTGAAGTGAACCAAGGCAGT
TTAATTAAAGGCAGTGCCGCAACTCACGCAGTATGTGGTATTGCTCTTCAGCGCGATATGGCGCTGATAAGAGTGGAGAG
TGAATCTTTCCCAAGCATCATCAAGCAGTGCCAAATGCTCGGCATTGAAGTCTGCACAACCATTGAAGAGCCTACCAATA
GTGCATTTGTGATTAAACGCGATGCTTATGCCAAGCTACAATTAGTCGGCGCAGATAAAATTCGTGGTTCAGAGCCCGTG
AGCCTCATCACCAAGGTCGGTATACAAGCCGGGGCATTAGTTGCCCATGCACGTCAGACCTTAGCGCAACATGGCATCGA
TGTGCGTTATGTCTCGGCCTCGGAACAATCATCTATGCTAATGTTAGACCCTGCAAATGTGGATCGTGCGGCAAATATTT
TGCACGAGACCTATGTGACGTCGGAAATCCCTCAAGAGTTTGGTCTAAAACAAACCTTTTTAGGTTAA

Upstream 100 bases:

>100_bases
GCCGCTTGGTTTTATTTTGGTGACGATCAAACCTTGTTCGACATGTATGTTAAGCAAGTTTGATCCCGATGAGACTTTGG
TCTTGGGAAGGTGAATACTG

Downstream 100 bases:

>100_bases
ACGGGCACCATTGAGAGTTTACGATGTCATAACTTTTGATGGATAATAGTGTGGTTGCAAGAAAATACAGAGATTACTCA
AGGAGCTAAGAATGCTTATT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKKPLIVQKFGGTSVGSIERMQTVAEHIIKAKNDGNQVVVVVSAMAGETNRLVGLAQQVDSVPNARELDVLLSAGEQVSM
ALVAMTLHKMGYAARSLTGAQANIVTDNQHNDATIKHIDTRTITELLEQDFIVIVAGFQGINENGDITTLGRGGSDTSAV
ALAGALNADECQIFTDVDGIYTCDPRIVPSARKLDVIDFPSMEEMARKGAKVLHLPCVQYAWKHAVPLRVLSTFEVNQGS
LIKGSAATHAVCGIALQRDMALIRVESESFPSIIKQCQMLGIEVCTTIEEPTNSAFVIKRDAYAKLQLVGADKIRGSEPV
SLITKVGIQAGALVAHARQTLAQHGIDVRYVSASEQSSMLMLDPANVDRAANILHETYVTSEIPQEFGLKQTFLG

Sequences:

>Translated_395_residues
MKKPLIVQKFGGTSVGSIERMQTVAEHIIKAKNDGNQVVVVVSAMAGETNRLVGLAQQVDSVPNARELDVLLSAGEQVSM
ALVAMTLHKMGYAARSLTGAQANIVTDNQHNDATIKHIDTRTITELLEQDFIVIVAGFQGINENGDITTLGRGGSDTSAV
ALAGALNADECQIFTDVDGIYTCDPRIVPSARKLDVIDFPSMEEMARKGAKVLHLPCVQYAWKHAVPLRVLSTFEVNQGS
LIKGSAATHAVCGIALQRDMALIRVESESFPSIIKQCQMLGIEVCTTIEEPTNSAFVIKRDAYAKLQLVGADKIRGSEPV
SLITKVGIQAGALVAHARQTLAQHGIDVRYVSASEQSSMLMLDPANVDRAANILHETYVTSEIPQEFGLKQTFLG
>Mature_395_residues
MKKPLIVQKFGGTSVGSIERMQTVAEHIIKAKNDGNQVVVVVSAMAGETNRLVGLAQQVDSVPNARELDVLLSAGEQVSM
ALVAMTLHKMGYAARSLTGAQANIVTDNQHNDATIKHIDTRTITELLEQDFIVIVAGFQGINENGDITTLGRGGSDTSAV
ALAGALNADECQIFTDVDGIYTCDPRIVPSARKLDVIDFPSMEEMARKGAKVLHLPCVQYAWKHAVPLRVLSTFEVNQGS
LIKGSAATHAVCGIALQRDMALIRVESESFPSIIKQCQMLGIEVCTTIEEPTNSAFVIKRDAYAKLQLVGADKIRGSEPV
SLITKVGIQAGALVAHARQTLAQHGIDVRYVSASEQSSMLMLDPANVDRAANILHETYVTSEIPQEFGLKQTFLG

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=216, Percent_Identity=33.7962962962963, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790455, Length=138, Percent_Identity=40.5797101449275, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790376, Length=206, Percent_Identity=27.6699029126214, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320893, Length=296, Percent_Identity=26.3513513513513, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 42461; Mature: 42461

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPLIVQKFGGTSVGSIERMQTVAEHIIKAKNDGNQVVVVVSAMAGETNRLVGLAQQVD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
SVPNARELDVLLSAGEQVSMALVAMTLHKMGYAARSLTGAQANIVTDNQHNDATIKHIDT
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECH
RTITELLEQDFIVIVAGFQGINENGDITTLGRGGSDTSAVALAGALNADECQIFTDVDGI
HHHHHHHHCCEEEEEEECCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCE
YTCDPRIVPSARKLDVIDFPSMEEMARKGAKVLHLPCVQYAWKHAVPLRVLSTFEVNQGS
EECCCCCCCCCCCEEEECCCCHHHHHHCCCEEEEEHHHHHHHHCCCCEEEEEEEECCCCC
LIKGSAATHAVCGIALQRDMALIRVESESFPSIIKQCQMLGIEVCTTIEEPTNSAFVIKR
EEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCHHHEEECCCCCCCEEEEEE
DAYAKLQLVGADKIRGSEPVSLITKVGIQAGALVAHARQTLAQHGIDVRYVSASEQSSML
CCCEEEEEEECHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEE
MLDPANVDRAANILHETYVTSEIPQEFGLKQTFLG
EECCCCCHHHHHHHHHHHHHHHCHHHHCCHHHCCC
>Mature Secondary Structure
MKKPLIVQKFGGTSVGSIERMQTVAEHIIKAKNDGNQVVVVVSAMAGETNRLVGLAQQVD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
SVPNARELDVLLSAGEQVSMALVAMTLHKMGYAARSLTGAQANIVTDNQHNDATIKHIDT
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECH
RTITELLEQDFIVIVAGFQGINENGDITTLGRGGSDTSAVALAGALNADECQIFTDVDGI
HHHHHHHHCCEEEEEEECCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCE
YTCDPRIVPSARKLDVIDFPSMEEMARKGAKVLHLPCVQYAWKHAVPLRVLSTFEVNQGS
EECCCCCCCCCCCEEEECCCCHHHHHHCCCEEEEEHHHHHHHHCCCCEEEEEEEECCCCC
LIKGSAATHAVCGIALQRDMALIRVESESFPSIIKQCQMLGIEVCTTIEEPTNSAFVIKR
EEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCHHHEEECCCCCCCEEEEEE
DAYAKLQLVGADKIRGSEPVSLITKVGIQAGALVAHARQTLAQHGIDVRYVSASEQSSML
CCCEEEEEEECHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEE
MLDPANVDRAANILHETYVTSEIPQEFGLKQTFLG
EECCCCCHHHHHHHHHHHHHHHCHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]